Objective To explore the effects of citicoline injection into Zusanli point (ST36) on learning and memory function of rats after traumatic brain injury (TBI). Methods The model was induced with the improved Feeney method. The rats were randomly divided into 5 groups: sham-operation group, control group, acupuncture point saline injection group, intraperitoneal drug injection group and acupuncture point drug injection group with 8 rats in each group. The rats in the acupuncture point drug or saline injection group were treated with acupuncture injection of citicoline or saline daily. The rats in the intraperitoneal drug injection group and control group were treated with intraperitoneal injection of citicoline or saline daily. The treatment continued for 14 d. The learning and memory function was evaluated with the Morris water maze test and passive avoidance test. Results Acupuncture point injection of citicoline can significantly shorten the escape latent period of TBI rats in Morris water tests and extend the latent period of learning and memory of TBI rats (P<0.05). Conclusion Acupuncture point injection is effective on the recovery of learning and memory function of rats after TBI.

Adult ; Female ; Humans ; Paraquat ; poisoning

Objective To observe the shape and the hemodynamic changes of the left heart after transcatheter closure of atrial septal defects(ASD) using Amplatzer occluder.Methods Sixty-five subjects with secondum ASD underwent transthoracic echocardiography(TTE) at pre-operation,48～72 h post-operation and follow-up performed more than 3 months after operation in 45 patients.The dimension of ASD,the parameters of the pulse-wave Doppler spectrums of the blood flow at the aotic valves and inlet of the left ventricle were recorded.At each position,systolic,early diastolic velocities and intervals were measured,the mitral annular movement spectrum was acquired at the lateral site of mitral annulus in the apical four-chamber view,Tei index and Tei_(TDI) index were derived from these parameters.Results All the patients had been implanted one Amplatzer occluder under the guide of echocardiography successfully.The preload of the left ventricular volume in the end of the diastolic period was increased and the low hemodynamic situation of the left heart and systemic circulation were corrected after the operation immediately.Accordingly,at 48～72 h after the operation,the end diastolic diameter,the stroke volume and the ejection fraction of the left ventricle were significantly improved continuously.However,the movement of the posterior wall decreased significantly at 48～72 h post-operation.The peak velocity of systolic period and the early diastolic velocities of the left ventricle were increased,but the ejection time had no changes follow-up.The systolic function of the left ventricle highlighted,the whole diastolic time prolonged after the 3 months of the operation.Tei index,Tei_(TDI) index and the heart rate had no difference between pre-and post-operation.Conclusions The anatomic deformation was corrected by transcatheter closure of ASD with Amplatzer occluder,the pre-load of left ventricular increased and had significant effect on the morphology and hemodynamic situation quickly,the left ventricular systolic and the diastolic function improved.The pre-load change of left heart had no effect on heart rate.Tei index and Tei_(TDI) index were independent of the pre-load and present the heart-self function.

AIM: To observe the effects of valproic acid(VPA) and HA14-1 on Bcl-2 overexpressed leukemia cells in vitro and in vivo.METHODS:(1) Cells were divided into control group,HA14-1 group,VPA group and HA14-1+VPA group.The apoptotic rate,the mean fluorescent index(MFI) of Bcl-2,the levels of caspase 3,8 and 9 were detected with FCM.(2) 24 h after transplantation with BALL-1,the NOD/SCID mice were divided into 4 groups as(1),then the survival time was compared.The expression of CD19 in the peripheral blood cells,bone marrows,livers,spleens and lungs of each group was detected.RESULTS:(1) The apoptotic rate in HA14-1+VPA group was(76.5?6.9)%,this was significantly elevated compared to the data in other groups(P

Objective To observe the influence of sodium citrate concentrations in TISAB on detection of water fluoride,and to explore the feasibility of 0.10 mol/L sodium citrate buffer system in detection of water fluoride.Methods Under pH 5.0 to 5.5,a series of fluoride standards of 0.2,0.5,1.0,2.0 and 5.0 mg/L were measured when sodium citrate concentration was 0.01,0.10,and 1.00 mol/L in the TISAB system.The results of water fluoride measurement were compared,recovery calculated and regression equation of the standard curve was set up.The feasibility of 0.10 mol/L sodium citrate in the TISAB system to detect water fluoride was tested,including interference test,accuracy,confidence limits,as well as precision test.Water fluoride was determined by fluoride ion selective electrode according to the Standard Test Methods for Drinking Water (GB/T 5750.5-2006).Results When the sodium citrate concentrations in the TISAB system were 0.01,0.10 and 1.00 mol/L,and the concentrations of fluoride in the sample were 0.2,0.5,1.0,2.0 and 5.0 mg/L,recoveries of water fluoride were 96.0％,103.0％,179.5％; 80.6％,97.8％,132.2％; 73.3％,97.0％,103.0％; 70.0％,100.0％,87.5％; and 66.4％,102.0％,65.4％.The equation of linear regression was y =lg-1(226.4-E/47.4),y =lg-1(226.4-E/53.4) and y =lg-1(208.1-E/36.9) ; the correlation coefficient(r) were 0.9993,0.9999 and 0.9993.The minimum detectable concentration and limit of quantitation was 0.023 and 0.072 mg/L when sodium citrate was 0.10 mol/L in the TISAB system.Aluminum(Al3+,100 μg),ferrum(Fe3+,800 μg),calcium(Ca2+,1200 μg),Al3+(50 μg) + Fe3+ (800 μg),Al3+(50 μg) + Ca2+(1200 μg) can be masked when sodium citrate was 0.10 mol/L in the TISAB system.The total average recoveries confidence limit R/d was 0.99.The total standard deviation of standard solution,water sample and spiked water sample was less than 5％ of their respective mean concentration.Conclusions High concentration of sodium citrate buffer system has a significant influence on the detection sensitivity and limit of quantification of fluoride ion in water.The capacity of anti-interference of 0.10 mol/L sodium citrate in the TISAB is strong.The confidence limits of precision and accuracy meet the requirements for fluoride determination in a variety of source water and drinking water.

Acupuncture Therapy ; Aged ; Aged, 80 and over ; Bulbar Palsy, Progressive ; etiology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Stroke ; complications

ObjectiveTo analyze the characteristics of top of the basilar syndrome (TOBS) in clinic and imaging.MethodsData of 31 TOBS cases were analyzed retrospectively.ResultsThe clinical features of TOBS patients were sudden unconsciousness or vertigo and dyskinesia of the limbs, the dismovement of the eyeballs, abnormality of the pupils, partial blindness or cortical blindness, hypesthesia, disturbance of memory and counting. CT and MRI showed multi-infarction included thalami, occipital lobe, cerebellum, midbrain, temporal lobe.ConclusionTOBS can be diagnosed accurately according to clinical features and imaging signs.

Objective: To establish the quality control method for Juhuang Oral Liquid (JOL) based on the qualitative and quantitative analysis methods. Methods: TLC was used to identify Lycii Fructus and Polygonati Rhizoma in JOL. Phenol-sulfuric acid method was used for the detemination of total polysaccharide, HPLC was used to simultaneously determine the contents of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, coffeic acid, galuteolin, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid. The Odyssil C18 (250 mm × 4.6 mm, 5 μm) column was used. The mobile phase was acetonitrile-0.05% phosphoric acid solution in gradient elution, at the flow rate of 1.0 mL/min, the detection wavelength was set at 325 nm. Results: The identified characteristics of Lycii Fructus and Polygonati Rhizoma by TLC were distinct and the spots were clear, without interference and easy to recognize. When phenol-sulfuric acid method was used to determinate the total polysaccharide content, the regression equation was A=0.057 8 C + 0.032 5 (r=0.999 1, n=8). The linear with its absorbance in the range of 26.15-196.13 mg/mL, the average recoveries was 99.82%. The average content of the total polysaccharide in JOL from six batches is 157.74-166.49 mg/mL. When RP-HPLC was used to determine the eight chemical components, the compounds showed a good linearity(r ≥ 0.999 8) in the determination range. The average recoveries were between 99.18%-101.06% with RSDs between 0.79%-1.91%. The average content from the six batches showed neochlorogenic acid 102.9-142.6 μg/mL, chlorogenic acid 488.6-563.8 μg/mL, cryptochlorogenic acid 58.9-71.6 μg/mL, coffeic acid 240.7-326.1 μg/mL, galuteolin 228.6-302.7 μg/mL, 3,4-dicaffeoylquinic acid 398.0-485.4 μg/mL, 3,5-dicaffeoylquinic acid 184.1-203.1 μg/mL, and 4,5-dicaffeoylquinic acid 476.2-561.8 μg/mL. Conclusion: TLC identification method is simple. The determination method of total polysaccharide and chemical components is simple, accurate, and with a good repeatability. It can be used for the quality control of JOL.

Objective To prohc into the distinguished feature of postoperation of early inflammatory intesti- nal and the principle of diagnosis and treatment.Methods 23 cases of early intestinal postoperatively treated during the year of 2003 to 2005 were analysed.Results In the 23 cases,20 of them were treated conservatively while 3 of them were treated operatively,both of which were recovered and dischanged from hospital.Conclusion The early inflammatory intestinal postoperatively is a kind of special one in which both mechanical factor and dynamic factor could be found.Most of the patients could be cured by conservative way and they should surely be in closed observa- tion of the condition in order to be handled properly.

Adolescent ; Adult ; Dermatitis, Occupational ; etiology ; therapy ; Drug Eruptions ; etiology ; therapy ; Female ; Humans ; Male ; Methylprednisolone ; therapeutic use ; Trichloroethylene ; adverse effects