1.Application value of combined detection of troponin I, creatine kinase isoenzyme mass, interleukin-6 and fibrinogen degradation products in the diagnosis and treatment monitoring of acute myocardial infarction
Chun XIAO ; Shuguo QIN ; Henggui HU
Chinese Journal of Postgraduates of Medicine 2021;44(1):21-28
Objective:To explore the application value of combined detection of troponin I (cTnI) and creatine kinase isoenzyme mass (CKMBmass), interleukin-6 (IL-6) and fibrinogen degradation products (FDP) in the diagnosis and treatment of acute myocardial infarction (AMI).Methods:A total of 102 AMI patients in Wanbei Coal Power Group General Hospital from July 2019 to June 2020 were selected as the AMI group. In addition, 60 patients diagnosed with chest pain (CP) and chest distress (CD) during the same period were selected as the CPCD group, and 60 healthy patients were selected as the healthy control group. The levels of cTnI, CKMBmass, IL-6 and FDP in the peripheral blood of the three groups were compared, and the receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of cTnI, CKMBmass, IL-6, and FDP. The AMI group was given thrombolytic therapy. The clinical data of patients with different curative effects, the trend of changes in peripheral blood cTnI, CKMBmass, IL-6 and FDP levels before and after treatment were compared, and the relationship between the above indicators and clinical indicators and curative effects were analyzed.Results:The levels of peripheral blood cTnI, CKMBmass, IL-6 and FDP in the AMI group were higher than those in the CPCD group and the healthy control group, and the levels of peripheral blood cTnI, CKMBmass, IL-6 and FDP in CPCD group were higher than those in the healthy control group ( P<0.05). The area under the curve (AUC) values of cTnI, CKMBmass, IL-6 and FDP in the combined diagnosis of CPCD and AMI were 0.898 and 0.926, respectively, which were higher than those of single diagnosis. The time from onset to thrombolysis, infarct location, diabetes, diastolic blood pressure (DBP), systolic blood pressure (SBP), left ventricular end systolic volume (LVESV), left ventricular end systolic diameter (LVESD), left ventricular end diastolic volume (LVEDV), left ventricular end diastolic diameter (LVEDD), left ventricular ejection fraction (LVEF) on admission of effective patients and ineffective patients were statistically significant ( P<0.05). The levels of peripheral blood cTnI, CKMBmass, IL-6, and FDP of effective patients were lower than those of ineffective patients when they were admitted to the hospital and 6, 12, 24, 48, and 72 h after treatment ( P<0.05). The peripheral blood cTnI, CKMBmass, IL-6, and FDP on admission were positively correlated with DBP, SBP, LVESV, LVESD, LVEDD, LVEDV, and was negatively correlated with LVEF ( P<0.05). Multiple linear stepwise regression analysis showed that after the onset to thrombolysis time, infarct location, smoking, diabetes, SBP, DBP, LVESV, LVESD, LVEDD, LVEDV, LVEF and other factors at admission were controlled, the levels of peripheral blood cTnI, CKMBmass, IL-6, FDP were still significantly related to the efficacy ( P<0.05). Conclusions:The peripheral blood cTnI, CKMBmass, IL-6, and FDP are all abnormally expressed in AMI patients. Combined detection has clinical significance for the diagnosis and treatment of AMI.
2.Analysis of β-thalassemia mutations in Guangdong province
Yu-xin, CHU ; Xiao-chun, WANG ; Zhao-hui, HU
Chinese Journal of Endemiology 2010;29(2):162-166
Objective To investigate the prevalence and spectrum of β-thalassemia mutations in C, uangdong province, and provide a reference for prenatal diagnosis and genetic counseling in this population. Methods Three thousand two hundred and forty-seven blood samples were randomly selected from Guangzhou and 2984 blood samples from Shenzhen from January in 2005 to January in 2009. PCR and reverse dot blot hybridization (RDB) were adopted for detection of β-thalassemia mutations in Guangzhou and Shenzhen city. Results Seven hundred and fifty-one individuals in Guangzhou were found to have β-hemoglobin gene mutations, the detection rate was 23.13%(751/3247); 10 different mutations were identified, namely CD41-42(-TCTT), IVS-Ⅱ-654(C→T), -28(A→G), CDI7(A→T), CD71-72(+A), 13E, IVS-I-1(G→T), CD43(G→T), -29(A→G), CDI4-15(+G), which accounted for 42.53% (336/790) ,25.19% (199/790), 12.66% (100/790), 10.89% (86/790) ,3.29% (26/790), 2.15%(17/790), 1.27%( 10/790), 1.14%(9/790) ,0.51%(4/790) ,0.38%(3/790), respectively; the most common mutation was CD41-42(-TCTT), which accounted for 42.53%(336/790). In Shenzhen, 179 individuals were found to have β-thalassemia mutations, the detection rate was 6.00% (179/2984); 8 different mutations were identified excluding CD43 (G→T) and CD14-15 (+G); the most common mutation, however, was IVS-lI--654(C→T), which accounted for 40.44% (74/183). Conclusions The β-thalassemia mutations in Guangdong province are not only frequent, but also obviously heterogeneous, and the mutations differ from region to region. CD41-42 (-TCTT),ⅣS-Ⅱ-654(C→T), -28(A→G), CD17(A→T) were the 4 predominant mutations.
4.Effect of down-regulation of nucleolin on adriamycin-induced apoptosis and inhibition of proliferation in breast cancer cells.
Xiao-cai TIAN ; Xian-ling LIU ; Chun-hong HU
Chinese Journal of Oncology 2010;32(4):271-272
Antibiotics, Antineoplastic
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pharmacology
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Apoptosis
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drug effects
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Breast Neoplasms
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metabolism
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pathology
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Down-Regulation
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Doxorubicin
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pharmacology
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Female
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Humans
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Oligonucleotides, Antisense
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pharmacology
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Phosphoproteins
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metabolism
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RNA-Binding Proteins
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metabolism
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Random Allocation
5.Expression of PED/PEA-15 and XIAP in prostate cancer cells and their effects on prostate cancer cell (PC-3) apoptosis
Xiao-Yong HU ; Xiao-Chun CHEN ; Zhao-Hui ZHU ; Fu-Qing ZENG ; Gong-Cheng LU ;
Chinese Journal of Geriatrics 2001;0(03):-
Objective To study the effect of antiapoptosis factors PED/PEA-15 and XIAP on prostate cancer cells(PC-3)apoptosis.Methods The expressions of XIAP and PED/PEA-15 in prostate cancer cells(PC-3)were respectively assayed using the RT-PCR technique.XIAP and PED/ PEA-15 specific siRNA vectors were designed and constructed and then were transiently cotransfected into PC-3 cells under induction of liposome.The effects of siRNA vectors on PED/PEA-15 and XIAP transcription were assayed by RT-PCR technique,and the effect of XIAP and PED/PEA-15 on cancer cell apoptosis were determined by flow cytometry and microscope observation.Results PED/PEA- 15 and XIAP were both highly expressed in PC-3 cells.Enzyme digestion analysis and DNA sequencing confirmed that the PED/PEA-15 and XIAP-specific siRNA expression vectors were constructed successfully.The designed siRNA sequences of PED/PEA-15 and XIAP could specifically inhibit their transcription.The PC-3 cells which were cotransfected with PED/PEA-15 and XIAP- specific siRNA vectors were more sensitive to doxorubicin.The apoptosis rate of cotransfected cells was significantly increased.Conclusions PED/PEA-15 and XIAP might be involved in the development of prostate cancer.
6.Correction of secondary lip whistle deformities and nasal base depression after bilateral cleft lip repair with lip subdermal soft tissue flap.
Xiao-Chen TIAN ; Xiao-Jie LÜ ; Xiao-Chun HU ; Jun-Nan CHEN ; Li-Long CUI ; Ying LIU ; Hui-Na LIU
Chinese Journal of Plastic Surgery 2013;29(3):164-167
OBJECTIVETo explore a new method to correct secondary lip whistle deformities and nasal base depression after bilateral complete cleft lip (BCCL) repair with lip subdermal soft tissue flap.
METHODSBilateral subdermal soft tissue "C" flaps and "lambda" flap were designed to repair secondary deformities of nasal base and reconstruct vermilion tubercle in patients after BCCL repair.
RESULTSGood results were achieved in all the patients with primary healing. No flap necrosis happened. The result was satisfactory.
CONCLUSIONSWith bilateral subdermal soft tissue "C" flaps and " lambda" flap, nasal base depression deformities and lip whistle deformities can be corrected. It is an ideal method for correction of deformities after BCCL repair.
Cleft Lip ; surgery ; Humans ; Lip ; surgery ; Nose ; Nose Deformities, Acquired ; surgery ; Reconstructive Surgical Procedures ; Surgical Flaps ; Treatment Outcome ; Wound Healing
7.Study on HPLC fingerprint of jinzhen oral solution.
Jun-Hua HU ; Qian-Qian SU ; Ting-Ting LI ; Jia-Chun LI ; Wei XIAO
China Journal of Chinese Materia Medica 2014;39(19):3768-3771
The HPLC fingerprint determination method of Jinzhen oral solution was established to provide a new method for quality control of Jinzhen oral solution. RP-HPLC was used for phenomenex Luna C18 (4.6 mm x 250 mm, 5 μm) chromatographic column, with 0.1% H3 PO4 water solution and acetonitrile as the mobile phase for gradient elution. The detection wavelength was 280 nm. HPLC fingerprint of Jinzhen oral solution was established to identify 17 common peaks in Jinzhen oral solution. The similarity of fingerprints of 10 batches of finished products was more than 0. 90. The established HPLC fingerprint has a better precision, reproducibility and stability, and can be applied in quality control of Jinzhen oral solution.
Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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chemistry
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Quality Control
8.Effect of electro-acupuncture stimulation of Ximen (PC4) and Neiguan (PC6) on remifentanil-induced breakthrough pain following thoracal esophagectomy.
Yan-Hu, XIE ; Xiao-Qing, CHAI ; Yue-Lan, WANG ; Yan-Chun, GAO ; Jun, MA
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(4):569-74
The clinical analgesic effect of electro-acupuncture (EA) stimulation (EAS) on breakthrough pain induced by remifentanil in patients undergoing radical thoracic esophagectomy, and the mechanisms were assessed. Sixty patients (ASAIII) scheduled for elective radical esophagectomy were randomized into three groups: group A (control) receiving a general anesthesia only; group B (sham) given EA needles at PC4 (Ximen) and PC6 (Neiguan) but no stimulation; and group C (EAS) electrically given EAS of the ipsilateral PC4 and PC6 throughout the surgery. The EAS consisting of a disperse-dense wave with a low frequency of 2 Hz and a high frequency of 20 Hz, was performed 30 min prior to induction of general anesthesia and continued through the surgery. At the emergence, sufentanil infusion was given for postoperative analgesia with loading dose of 7.5 μg, followed by a continuous infusion of 2.25 μg/h. The patient self-administration of sufentanil was 0.75 μg with a lockout of 15 min as needed. Additional breakthrough pain was treated with dezocine (5 mg) intravenously at the patient's request. Blood samples were collected before (T1), 2 h (T2), 24 h (T3), and 48 h (T4) after operation to measure the plasma β-EP, PGE2, and 5-HT. The operative time, the total dose of sufentanil and the dose of self-administration, and the rescue doses of dezocine were recorded. Visual Analogue Scale (VAS) scores at 2, 12, 24 and 48 h postoperatively and the incidence of apnea and severe hypotension were recorded. The results showed that the gender, age, weight, operative time and remifentanil consumption were comparable among 3 groups. Patients in EAS group had the lowest VAS scores postoperatively among the three groups (P<0.05). The total dose of sufentanil was 115±6.0 μg in EAS group, significantly lower than that in control (134.3±5.9 μg) and sham (133.5±7.0 μg) groups. Similarly, the rescue dose of dezocine was the least in EAS group (P<0.05) among the three groups. Plasma β-EP levels in EAS group at T3 (176.90±45.73) and T4 (162.96±35.00 pg/mL) were significantly higher than those in control (132.33±36.75 and 128.79±41.24 pg/mL) and sham (136.56±45.80 and 129.85±36.14 pg/mL) groups, P<0.05 for all. EAS could decrease the release of PGE2. Plasma PGE2 levels in EAS group at T2 and T3 (41±5 and 40±5 pg/mL respectively) were significantly lower than those in control (64±5 and 62±7 pg/mL) and sham (66±6 and 62±6 pg/mL) groups. Plasma 5-HT levels in EAS group at T2 (133.66±40.85) and T3 (154.66±52.49 ng/mL) were significantly lower than those in control (168.33±56.94 and 225.28±82.03) and sham (164.54±47.53 and 217.74±76.45 ng/mL) groups. For intra-group comparison, plasma 5-HT and PGE2 levels in control and sham groups at T2 and T3, and β-EP in EAS group at T3 and T4 were significantly higher than those at T1 (P<0.05); PGE2 and 5-HT levels in EAS group showed no significant difference among the different time points (P>0.05). No apnea or severe hypotension was observed in any group. It was concluded that intraoperative ipsilateral EAS at PC4 and PC6 provides effective postoperative analgesia for patients undergoing radical esophagectomy with remifentanil anesthesia and significantly decrease requirement for parental narcotics. The underlying mechanism may be related to stimulation of the release of endogenous β-EP and inhibition of inflammatory mediators (5-HT and PGE2).
9.Application of an HPLC correction factor method in pharmaceutical analysis
Ting XIAO ; Chen WANG ; Shang-chen YAO ; Yan-chun FENG ; Chang-qin HU
Acta Pharmaceutica Sinica 2020;55(12):2854-2861
With the in-depth study of related substances and the development of consistency evaluation of generic drugs, relative correction factors are gaining increasing attention. By analyzing the domestic and foreign literature on correction factors in recent years, this paper describes the correction factor component, the current measurement method and its application. The rules and key points of use of an impurity correction factor and its determination and application are described, and some problems in its determination and application are discussed, providing a reference and basis for the standardization of research on impurity correction factors in the future.
10.Direct medical costs of tuberculosis patients and its influencing factors in Hainan Province
WANG Fan ; HU Xiao-jie ; SU Zhu-na ; LIU Chun-ping
China Tropical Medicine 2023;23(5):506-
Abstract: Objective To investigate and analyze the factors influencing the direct medical costs of tuberculosis patients in Hainan Province, so as to provide scientific reference for reducing the medical burden of patients and adjusting the medical insurance reimbursement policies in the local area. Methods Using the total health expenditure accounting data of Hainan Province in 2020, including the outpatient and inpatient data of 14 provincial medical institutions, 235 city and county level medical institutions, and other relevant data from the 2020 Hainan Statistical Yearbook and Health Financial Yearbook, the direct medical costs of tuberculosis patients in the province in that year were calculated, and the influencing factors were explored using single factor analysis and multivariate generalized linear model. Results The final number of cases included in this study was 11 979, including 7 526 males (62.83%) and 4 453 females (37.17%). The total direct medical costs of patients were 43.207 3 million yuan, of which the total outpatient costs were 2.733 9 million yuan (6.32%) and the total inpatient costs were 40.473 4 million yuan (93.67%). In the cost composition analysis, the drug cost was 17.971 million yuan (41.44%), the examination cost was 8.854 7 million yuan (20.49%), other costs were 16.445 5 million yuan (38.06%), and the median (quartile) M(P25,P75) direct medical cost of each patient was 177.50 (66.73,764.89) yuan. The multivariate generalized linear model analysis showed that hospitalization, new rural cooperative medical insurance (NRCMI) and urban employee medical insurance were the influencing factors of the increase in direct medical costs of tuberculosis patients the median (quartile) M(P25,P75) of direct medical costs are 10 425.04 (6 560.87,17 374.9), 10 246.5 (5 871.28,17 220.33), 3 177.2 (293.09,7 730.23) yuan respectively; the OR(95%CI) values were -3.505 (-3.499- -3.517), 1.559 (1.551-1.569) and 2.191 (2.188-2.207) respectively. Conclusions The direct medical costs of tuberculosis patients in Hainan Province are high. Hospitalization, the new rural cooperative medical insurance and the medical insurance for urban workers are the influencing factors of the increase in costs.