2.Randomized-controlled trial of anti-scarring effectiveness on filtrating surgery combined with amniotic membrane
Yen-Ling, CHO ; Ping, HUANG ; Chun, ZHANG
Chinese Journal of Experimental Ophthalmology 2013;(3):265-269
Background The scar formation of filtering bleb is a cause of filtering operation failure.The topical application of mitomycin C (MMC) can inhibit the growth of collagenous fiber and improve function of bleb.However,some adverse effects of MMC are being closely concerned.Many studies have conformed the efficacy of amniotic membrane in filtering operation,but the comparison study between MMC and amniotic membrane is still lack.Objective Purpose of this study was to evaluate the anti-scarring effectiveness of amniotic membrane after its use in the filtrating surgery.Methods A randomized,controlled trial was designed.This study was approved by Ethic Committee of Peking University Third Hospital Eye Center.Written informed consent was obtained from each patient initial of this study.Fifty-two eyes of 47 patients with primary glaucoma were included in Peking University Third Hospital Eye Center from October 2009 to October 2011.The eyes were divided into two groups according to single blindness design and randomized digital table.Trabeculectomy combined with amniotic implantation was performed in the patients of the amniotic group,and trabeculectomy combined with 0.02% MMC in the patients of the MMC group.The patients were followed-up for 3 months.Intraocular pressure (IOP),the anterior chamber depth,filtering bleb shape,incidence of complication and operative efficacy were measured and evaluated.Results No significant difference was found in the demography between two groups (P>0.05).Seventeen patients in the MMC group and twenty-two patients in the amniotic group finished the trial.The IOP in the amniotic membrane group was (16.60-±5.04) mmHg on the third month after operation and that before operation was(23.52±-6.52) mmHg.In the MMC group,the IOP was(24.09± 12.79) mmHg on the third months after operation,and that before operation was (17.04±3.69) mmHg.Significant difference was found in the IOP between preoperation and postoperation in both groups(P<0.05).IOP differential value on the third month was 6.98 mmHg in the amniotic membrane group and 6.94 mmHg in the MMC group.No significant differences were seen in the IOP between the amniotic membrane group and the MMC group in 1 week,2 weeks,1 month and 3 months(t=0.972,P=0.336;t=-0.512,P=0.611;t =-0.372,P =0.712 ; t =0.427,P =0.672).There were no significant differences in the number of eyes in the height grade,vascularization and perimeter classification of the filtering blebs 1 week,2 weeks and 1 month after surgery between these two groups (x2 =0.991,P =0.320 ; x2 =0.474,P =0.491 ; x2 =2.008,P =0.156).The total effective rate of surgery was 90.49% in the amniotic membrane group and 100% in MMC group,with a significant difference between them(x2 =26.180,P=0.000).There were not significantly different in the incidences of bleb leakage in 1 week,2 weeks and 1 month after operation between the MMC group and the amniotic group(x2 =3.556,P =0.059;x2=1.129,P=0.800; x2=0.434,P =0.510).Conclusions Combination of trabeculectomy with amniotic implantation has a similar lowing-IOP effect to trabeculectomy combined with MMC.The incidence of bleb leakage in the trabeculectomy combined with amniotic implantation dose not increase when compare with trabeculectomy combined MMC.
4.Clinical regularities in acupuncture-moxibustion treatment of cancer pain in recent 30 years
Qin-Feng HUANG ; Chen XIE ; Chun-Ling TANG ; Zhu JIN
Journal of Acupuncture and Tuina Science 2019;17(2):89-98
Objective:To explore the clinical regularities in acupuncture-moxibustion treatment of cancer pain by reviewing the relevant studies published between 1985 and 2017.Methods:Based on the Chinese Medicine Acupuncture-moxibustion Information Database,quantitative analysis,correlation analysis and Chi-square test were applied to analyze the commonly used acupoints,meridian affiliations and body region distributions,commonly used methods and acupoint correlations,treatment method correlations,the efficacy of acupuncture-moxibustion plus the three-step analgesic ladder for cancer pain,and indicators.Results:Zusanli (ST 36),Ashi point and Sanyinjiao (SP 6) ranked the top on the list of frequency;points from Bladder Meridian of Foot Taiyang and Stomach Meridian of Foot Yangming were often used;points from the lower limbs and back had high frequencies.The most commonly used treatment method was acupuncture-moxibustion plus medication.Acupuncture-moxibustion plus the three-step analgesic ladder showed certain advantage compared with the two methods used separately,and among the integrated methods,acupoint application plus the three-step analgesic ladder produced the most significant efficacy.It took (44.77±55.54) min for the analgesic effect to act and the effect lasted for (12.81±14.59) h.Numerical rating scale (NRS),visual analog scale (VAS) and Karnofsky performance status (KPS) scores all showed significant changes after interventions (all P<0.01);there was no significant change in the score of quality of life (QOL) after interventions (P>0.05).Conclusion:Zusanli (ST 36),Ashi point and Sanyinjiao (SP 6) are commonly selected in acupuncture-moxibustion treatment of cancer pain;acupuncture and acupoint application are often used;acupuncture-moxibustion plus the three-step analgesic ladder can boost the treatment efficacy.
5.Preventive Effect on Nosocomial Infection with Herbal Medicine Atractylodes chinensis Disinfectant for Air Sterilization in Cardiothoracic Surgery
Hong-Yan HE ; Li SHENG ; Xue-Qin HUANG ; Fu-Ling LV ; Yuan-Chun HUANG ;
Chinese Journal of Nosocomiology 1994;0(04):-
OBJECTIVE To observe the preventive effect on nosocomial infection by the herbal medicine Atractylodes chinensis disinfectant for air sterilization in cardiothoracic surgery.METHODS A.chinensis disinfectant and ultraviolet irradiation were used to disinfect the patients′ rooms of our cardiothoracic surgery department.The total number of germ and fungus in the indoor air before and after using the two methods were collected.The nosocomial infection rates of the same period were also studied.RESULTS A.chinensis disinfectant had strong effect on sterilization of indoor germs and fungi,and the sterilization rate was 92.7%.The comparison between A.chinensis disinfectant and ultraviolet irradiation showed significant difference in their disinfection effects,while no significant difference in their nosocomial infection rates.CONCLUSIONS The herbal medicine A.chinensis disinfectant can achieve good sterilization effect and prevent nosocomial infection.
6.Quality analysis of Guizhi Fuling capsule before and after application of in-process quality control in pharmaceutical production.
Jia-chun LI ; Jin-ling WANG ; Jing-ling WU ; Wen-zhe HUANG ; Zhen-zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(6):1017-1022
The effects of application of in-process quality control in Guizhi Fuling capsule production were evaluated by 192 batches data analysis. Using a statistical analysis method, each batch of data were to be counted to research for the difference between 96 samples adopting the technologies of in-process control or not. According to quality standards of Guizhi Fuling capsule, all measurements of the 192 batches of the drugs before and after the application of process control technology were analyzed, and they were within the rules. There was a significant difference between adopting the technologies of process control or not. Application of in-process control technology can improve the uniformity of lot-to-lot for Guizhi Fuling capsule.
Capsules
;
chemistry
;
Chemistry, Pharmaceutical
;
Chromatography, High Pressure Liquid
;
Drugs, Chinese Herbal
;
chemistry
;
Quality Control
7.Effects of jingjin acupuncture on fine activity of hemiplegic hand in recovery period of stroke.
Yan-Ling ZHAO ; Wen-Chun LI ; Juan HUANG ; Zai-Li FU ; Ling-Qiong TAN ; Zhao-An TANG ; Jun-Feng HE
Chinese Acupuncture & Moxibustion 2014;34(2):120-124
OBJECTIVETo observe improved effects of Jingjin acupuncture on fine activity of hemiplegic hand in recovery period of stroke.
METHODSFifty cases were randomly divided into an observation group and a control group, 25 cases in each one. Regular western medicine treatment, rehabilitation training and regular acupuncture (in which Shuigou (GV 26), Baihui (GV 20), Neiguan (PC 6), etc. were selected) were applied in both groups. Additionally, muscles in palm side of affected hand, dorsal metacarpophalangeal joints and proximal interphalangeal joints were treated with acupuncture in the observation group, once every other day and electroacupuncture was applied when arrival of qi was acquired. Baxie (EX-UE 9) in the affected hand were needled in the control group, and electroacupuncture was added when arrival of qi was acquired. Ten days of treatment was considered a treatment course, and after two courses Lindmark score, Brunnstrom movement function grade, joint range of hand and Barthel index (BI) were observed in two groups.
RESULTSCompared before the treatment, the Lindmark score in two groups were both improved after the treatment (both P < 0.01). Compared with the control group, the motor coordination ability, sensory function and total score of Lindmark in observation group were obviously improved (differences before and after treatment: 8.24 +/- 3.07 vs 6.84 +/- 2.43, 3.52 +/- 2.33 vs 2.16 +/- 2.12, 11.76 +/- 3.55 vs 9.00 +/- 3.62, all P < 0.05). The Brunnstrom movement function grade was significantly improved in both groups after treatment (both P < 0.01), which was more obvious in the observation group (P < 0.05). The joint range of hemiplegic hand was improved in both groups after treatment (both P < 0.01), which was more obvious in the observation group [differences before and after treatment: (25.35 +/- 10.91) degrees vs (18.65 +/- 7.86) degrees, p < 0.05]. The score of BI was also significantly improved after treatment in two groups (both P < 0.01).
CONCLUSIONThe Jingjin acupuncture could effectively improve fine activity of hemiplegic hand in recovery period of stroke prove daily life ability.
Acupuncture Therapy ; Aged ; Female ; Hand ; physiopathology ; Hemiplegia ; etiology ; physiopathology ; therapy ; Humans ; Male ; Middle Aged ; Movement ; Recovery of Function ; Stroke ; complications ; physiopathology
9.Cloning, Expression and Property Analysis of Arabinosidase in Pichia pastoris
Dong-Sheng YAO ; Hui-Mei TAN ; Hui HUANG ; Da-Ling LIU ; Chun-Fang XIE ;
China Biotechnology 2006;0(04):-
SMART-RACE was performed after isolating the total RNA of Armillariella tabescens to amplify the full-length cDNA of arabinosidase (GenBank Accession No. AJ620046). Bioinformatics analysis was used to analyze the code frame of arabinosidase, to predict its structure and function. Recombinant plasmid pPIC9-AF was constructed and then electroporated into methylotrophic yeast Pichia pastoris GS115. The secreted 6 ? His fusion protein was purified to analyze its enzymology property. This arabinosidase had high activity at 30-35℃ under acid condition, and was stable within wide range of pH and temperature. It maintained about 80% activity at the range of pH4. 0-8.0 and 20-40℃,wider than many other cloned arabinosidase. So it was worthy to go step further to study this enzyme, and recombinant expression provided a chance of highly expressing arabinosidase.
10.Inducement, Purification and Characterization of?-mannanase from Armillariella tabescens EJLY2098
Dong-Sheng YAO ; Xiao-Kui HUANG ; Da-Ling LIU ; Chun-Fang XIE ; Rong HU ;
China Biotechnology 2006;0(07):-
Armillariella tabescens EJLY2098 was capable of secreting p-mannanase by konjac inducement. A 34 orthogonal design was applied to determine the optimum medium of inducing mannanase by Armillariella tabescens EJLY2098. The results suggested that Armillariella tabescens EJLY2098 secreted the high-activity enzyme in the optimum medium, which was composed of 2% konjac, 1% peptone, 25% potato juice,0.3% KH2PO4,15% MgSO4?7H2O, 0.01% VitB1. Purified by DEAE-anion exchange chromatography, two eluting peaks (P1 and P2) with the p-mannanase activity were obtained, and one of them (named?-mannanase P2) was a single band by the SDS-PAGE, and the molecular weight of?-mannanase P2 was 78. 9kDa. The isoelectric point of?-mannanase P2 was estimated to be 4.0-4. 1. The optimum activity for the enzyme was found at 60℃and pH4. 0 - 6. 0, and the enzyme was stable between pH4. 5 - 6. 0. The activity of?-mannanase P2 were enhanced by Na+ and Ba2+ . This?-mannanase can be used in feed industy. a new fungi secreting?-mannanase was obtained, providing an important base for cloning mannanase gene and constructing recombin microbe expressing?-mannanase .