The distribution and morphology of supraependymal cells residing within thethird ventricle of the adult rabbit and adult rat have been investigated by means ofscanning electron microscopy.Supraependymal cells can be classified into twofundamental types,those with neuronal characteristics were designated Type Ⅰ incontrast to those that appear to be macrophagic in character,which were termedType Ⅱ.The neuron-like supraependymal cells have oval cell bodies and long slen-der processes.They occurred not only singly along the ventricular surface,but alsotended to occur in clusters which may be named as neuronal complex.Usually theneuronlike structures possessed varicose fibers of various calibers,runing among thecilia,microvilli,and bulbous protrusions of the ependymal surface.The morphologyof macrophage-like ependymal cells are variable.In general,they had one or severalbroad pseudopodia.According to the number of processes,the macrophage-likesupraependymal cells may be divided into three main types.The morphology of supraependymal cells within the third ventricle of the rabbitand rat is quite similar.The role of supraependymal cells were discussed.

Arm Injuries ; etiology ; Autonomic Pathways ; pathology ; Central Nervous System ; pathology ; China ; Humans ; Occupational Diseases ; etiology ; Temperature ; Vibration ; adverse effects

Objective To investigate the expression of estrogen receptor (ER), progesterone recptor (PR) and c-erbB-2 in breast cancer tissues and its relationship with prognostic factors. Methods The clinical data of 1 008 cases with breast cancer were retrospectively analysed. The relationship between the expression of ER, PR and c-erbB-2 in breast cancer tissues and the prognostic factors such as menopausal status, axillary lymph nodes metastasis and TNM stage was investigated. Results The mean presentation age of the 1 008 cases was (53.53?12.60) years. Invasive ductal cancer was the most common pathological type (80.5%). Postmenopausal ER-positive cases were significantly more than premenopausal ER-positive ones, and the clinical stage was lower (P

Antibodies, Monoclonal ; therapeutic use ; Antibodies, Monoclonal, Murine-Derived ; Antigens, CD20 ; immunology ; Antineoplastic Agents ; therapeutic use ; Child, Preschool ; Female ; Humans ; Lymphoma, B-Cell ; drug therapy ; Rituximab ; Treatment Outcome

Objective To explore the feasibility and to improve the efficiency of testing circulating tumor cells(CTC)in patients with renal cell carcinoma by Cell-search System(CSS).Methods Eight patients with renal cell carcinoma hospitalized in the PUMC urology department for further clinical evaluation in Jan.to Jun.2012 were enrolled in this study.There were 5 males and 3 females with mean age of 64 (57-70)years.There were 2 cases in clinical stage T3N0M0 and 6 cases in T4N0M1;3 cases were treated with radical nephrectomy,5 cases were treated with targeted therapy drugs.7.5 ml peripheral blood samples from these patients were collected and saved in test tube at the temperature of 15 ℃-30 ℃.The tests had to be done within 96 hrs after the blood sample drawn.We selected fluorescent anti-CKS/18/19 antibodies as CTC makers for renal cell carcinoma.Then the number of CTC was quantitative detected by CSS.The feasibility and detection rate of testing CTC in patients with RCC by CSS was evaluated,and the improvement of method was discussed.Results Quantitative test results of CTC in 8 patients with renal cell carcinoma were negative.25 CK+CD45 + double positive cells were found in one T4N0M1 patient's peripheral blood.The detection rate of the CTC in TNM stage T3N0M0 and T4N0M1 renal cell carcinoma patients using CSS with epithelial tumor cell maker anti-CK8/18/19 was zero.Conclusions Anti-CK8/18/19 antibodies can not be used in testing circulating tumor cells in patients with renal cell carcinoma by Cell-search System.

As a member of tyrosine kinases(TK) family,Epidermal growth factor receptor(EGFR) has an activity of intrinsic protein tyrosine kinase,which plays an essential role in the regulation of signal transduction in the cells.Due to the abnormal expression of EGFR-TK,the certain type cancers may developed and progressed.Based on that,the inhibitors of EGFR-TK could be effective medicines for the treatment of cancer.The EGFR-TK domain was amplified by RT-PCR with RNA of HUVCEs cells as the template and expressed in E.coliBL21(DE3) using plasmid pET30a as vector.The recombinant protein was purified with the affinity chromatography(Ni-NTA),which was identified to have kinase activity catalyzing the substrate phosphorylated with ATP in the enzymatic reaction.Using the recombinant EGFR-TK as target,the screening model for enzymatic inhibitors was constructed.

Objective To investigate the expression of miR-200c in endometrial carcinoma and its correlation with ZEB2 protein. Methods The expression levels of miR-200c and ZEB2 gene and protein in tissues were detected. The expression of miR-200c in endometrial carcinoma RL95-2 cell line was inhibited by using antisense oligonucleotides and its effect on cell invasiveness was tested. The expressions of ZEB2 gene and protein in cells were detected. Results The expression levels of miR-200c and ZEB2 mRNA and protein in endometrial tissues were significantly higher than those in adjacent tissues and control group (P < 0.05). The average penetrating number in antisense miR-200c transfected group was significantly less than negative control group and liposome group (P < 0.05). The expression levels of ZEB2 gene and protein in antisense miR-200c transfected group were lower than the negative control group and liposome group (P < 0.05). Conclusion The expression of mRNA-200c in endometrial carcinoma was high and it might be promoting tumor cell invasion and metastasis by regulating ZEB2.

Objective To study the effect of quercetin on proliferation and expression of P38MAPK and HMGB1 protein in neonatal rat cardiac fibroblasts induced by TNF-α.Methods Purified cardiac fibroblasts were obtained by trypsin digestion and differential adherence method.The proliferation of cardiac fibroblasts was detected by MTT assay.The expression of P38MAPK and HMGB1 protein was detected by Western blot.Results Quercetin had no effect on the proliferation of cardiac fibroblasts in the basal state but inhibited the proliferation of cardiac fibroblasts induced by TNF-α,and the A value of each group was increased with the increase of quereetin concentration(P＜0.05).The exspression of P38MAPK、HMGB1 were decresed with the icrease of quercetin.Conclusion Quercetin may inhibit the proliferation of cardiac fibroblasts induced by TNF-α.The mechanism may be inhibit the expression of HMGB1 through P38MAPK signaling pathway.