OBJECTIVETo identify the binding proteins to PDZ domain of ERBIN.

METHODSUsing PDZ domain of ERBIN as the bait, yeast two-hybrid technology was employed to screen the human lymphocyte leukemia cells MATCHMAKER cDNA library. The protein interaction was identified by immunoprecipitation.

RESULTSA PDZ-binding protein, TAX1, was identified.

CONCLUSIONTAX1 is a novel binding protein to PDZ domain of ERBIN.

Adaptor Proteins, Signal Transducing ; metabolism ; Cell Line, Tumor ; Humans ; Immunoprecipitation ; Intracellular Signaling Peptides and Proteins ; metabolism ; Neoplasm Proteins ; metabolism ; PDZ Domains ; Protein Binding ; Two-Hybrid System Techniques

Objective To investigate the pregnant women's mental status of the earthquake disaster area,and find the nursing measures to intervene the pregnant women and reduce the influence. Methods 106 pregnant women in the earthquake and none earthquake area were investigated respectively using SAS and SDS. Results The pregnant women's mental status was notably influenced by the earthquake. The incidence of anxiety, depression were higher than that in the non-earthquake area (P < 0.01). The incidence of anxiety, depression among pregnant women whose direct relatives was the earthquake victims were higher(P <0.05). The level of the pregnant women's anxiety and depression status decreased after nursing intervenes (P < 0.01). Conclusions The earthquake hurt the pregnant women's mental health, nurse intervention can improve their anxiety and depression status, make them be in the best mental health and somatic health, help their childbirth and make the mother and newborns heath.

Objective To discuss the improving effect of tenuigenin on learning and memory ability and its influence on cerebral monoamine neurotransmitters in senescence-accelerated mice prone 8(SAMP8).Methods The senescence-accelerated mice(SAMP8)were chosen as subjects,and then randomly divided into the model group,tenuigenin group and Donepezil group.SAMR1 were chosen into the the control group.After intragastrical treatment for 3 months,the learning and memory ability and the levels of 5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA),dopamine(DA)and norepinephrine(NE)were detected respectively by applying Morris water maze and high performance liquid chromatography(HPLC).Results In the tenuigenin group the hidden platform test showed that the escape latency was,respectively,(43.56±10.87)s,(41.37±9.71)s,(37.62±10.05)s and (41.60±13.61)s from the 2nd to 5th day,and the reversed test showed that the escape latency was (37.10±14.04)s on the 9th day,which were all shortened compared with those in the model group,and the difference was statistically significant(P＜0.05).In the tenuigenin group the level of 5-HT,5-HIAA,DA or NE was,respectively,(5.95±0.93)mmol/L,(4.43±1.08)mmoL/L,(2.61±0.87)mmol/L and(9.44±3.76)mmol/L,which all increased compared with those in the model group,and the difference was statistically significant(P＜0.05).Conclusion Tenuigenin can improve the learning and memory ability of SAMP8,and the effect may related to that it can regulate the level of cerebral monoamine neurotransmitters.

Myelodysplastic Syndromes(MDS) comprise a heterogenous group of hematopoietic stem cell malignancies characterized by peripheral cytopenias and have a substantial risk of progression to acute myeloid leukemia(AML). MDS, without effective cure methods, is one of the common hematologic malignant tumors with great threaten to people's health. The phenomenon of iron overloading is common in MDS, which has a poor effect on overall survival and leukemic progression to MDS but get good prognosis by iron chelation therapy. Therefore, increasing researchers are interested in iron overloading of MDS. So far, many researchers have reported that blood transfusion, ineffective hematopoiesis, genetic changes, mitochondrial apoptosis and ROS were found to be important in the incidence of iron overloading. There is greatly valuable to guide iron chelation therapy to study the relationship between those elements with iron overloading. In this paper, we reviewed the great important and specific influence of blood transfusion, ineffective hematopoiesis, genetic changes, mitochondrial apoptosis and ROS in the mechanism of iron overloading, which there is a great significance on iron overloading- associated MDS.

BACKGROUNDThe neurogenic bladder dysfunction caused by spinal cord injury is difficult to treat clinically. The aim of this research was to establish an artificial bladder reflex arc in rats through abdominal reflex pathway above the level of spinal cord injury, reinnervate the neurogenic bladder and restore bladder micturition.

METHODSThe outcome was achieved by intradural microanastomosis of the right T13 ventral root to S2 ventral root with autogenous nerve grafting, leaving the right T13 dorsal root intact. Long-term function of the reflex arc was assessed from nerve electrophysiological data and intravesical pressure tests during 8 months postoperation. Horseradish peroxidase (HRP) tracing was performed to observe the effectiveness of the artificial reflex.

RESULTSSingle stimulus (3 mA, 0.3 ms pulses, 20 Hz, 5-second duration) on the right T13 dorsal root resulted in evoked action potentials, raised intravesical pressures and bladder smooth muscle, compound action potential recorded from the right vesical plexus before and after the spinal cord transaction injury between L5 and S4 segmental in 12 Sprague-Dawley rats. There were HRP labelled cells in T13 ventral horn on the experimental side and in the intermediolateral nucleus on both sides of the L6-S4 segments after HRP injection. There was no HRP labelled cell in T13 ventral horn on the control side.

CONCLUSIONUsing the surviving somatic reflex above the level of spinal cord injury to reconstruct the bladder autonomous reflex arc by intradural microanastomosis of ventral root with a segment of autologous nerve grafting is practical in rats and may have clinical applications for humans.

Anastomosis, Surgical ; Animals ; Atropine ; pharmacology ; Male ; Models, Theoretical ; Rats ; Rats, Sprague-Dawley ; Reflex, Abdominal ; drug effects ; physiology ; Trimethaphan ; pharmacology ; Urinary Bladder, Neurogenic ; physiopathology

OBJECTIVETo investigate the relationship of FGA gene 128C/G polymorphism and cerebral infarction (CI) and evaluate the effect of FGA-128C/G polymorphism on plasma fibrinogen in Hunan Hans.

METHODSFGA-128C/G polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing in 194 CI patients and 114 healthy controls.

RESULTSThere were CG and CC genotypes in the FGA-128C/G locus. No GG genotype was observed in Hunan Hans. There was no significant difference in genotype and allele frequencies between the controls and CI group (P> 0.05), and statistically significant difference was not found in fibrinogen (Fg) level between the CG and CC genotypes (P>0.05). After analyzing blood plasma Fg using the influencing factor multiple regression analysis, it was shown that the Fg level had no relationship with the FGA-128C/G genotype, but it increased with age. And the Fg level in males was higher than that in females.

CONCLUSIONThere was FGA gene 128C/G polymorphism in the Hunan Han population. There was no association of this polymorphism with the increased Fg level of CI patient in the population. FGA-128C/G might not be the predisposing gene of CI in Hunan Han population. The age and sex were the major factors affecting the plasma Fg level in this population.

Aged ; Asian Continental Ancestry Group ; genetics ; Cerebral Infarction ; genetics ; Female ; Fibrinogen ; genetics ; Gene Frequency ; genetics ; Genetic Predisposition to Disease ; genetics ; Genotype ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Genetic ; genetics ; Polymorphism, Restriction Fragment Length

OBJECTIVETo investigate the clinical therapeutic results of allograft tendon for anatomical reconstruction of medial patellofemoral ligament (MPFL) for the treatment of patellar dislocations.

METHODSFrom September 2008 to June 2013, 16 patients with patellar dislocation underwent MPFL reconstructions. There were 2 males and 14 females, aged 11 to 27 years old (16 years old on average). Patellar dislocations occurred in 11 left and 5 right knees. The disease course ranged from 3 to 10 years. The frequency of dislocation ranged from 9 to 33 times (19 times on average). Affected knee joints showed patellar instability; the range of action for patella obviously increased. The X-ray films showed patellar dislocation. The preoperative Q angle was (36 ± 9)°, and the congruence angle was (63 ± 18)°. Reconstruction was performed via allograft tendon. Allograft tendon was fixed through the superomedial pole of the patella, and the other end was fixed at the natural MPFL insertion site near the medial femoral condyle with an interference screw in a bone tunnel. All the patients were evaluated postoperatively; Kujala patellofemoral scores, objective knee function, complications, and reoperations were assessed.

RESULTSPrimary healing was achieved in all cases. No infection or necrosis and absorption of grafts was observed. All the patients were followed up for an average of 16.4 months (ranged, 10 to 24 months) postoperatively. At the latest follow-up, all the patients had no pain, swelling and patellar instability; neither patella redislocation nor fracture occurred. The X-ray films showed good position of tunnel 6 months after operation, and the congruence angle was (5 ± 9)°, showing statistically significant difference when compared with preoperation (P < 0.05). The postoperative Q angle was (17 ± 8)°, the Kujala knee function score improved significantly from 45.20 ± 9.20 to 89.30 ± 6.40 at the latest follow-up, showing statistically significant difference (P < 0.05).

CONCLUSIONMPFL reconstruction improves clinical symptoms. Anatomical MPFL reconstruction is effective for patellar dislocation, and it offers good recovery of the premorbid patella mechanics. The interference screw provides firm fixation. Allograft can avoid the graft harvest site morbidity, but it increases the cost of the surgery.

Adolescent ; Adult ; Allografts ; Child ; Female ; Humans ; Ligaments, Articular ; surgery ; Male ; Patellar Dislocation ; surgery ; Patellofemoral Joint ; surgery ; Reconstructive Surgical Procedures ; methods ; Tendons ; transplantation

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Intracranial Hemorrhages ; diagnosis ; therapy ; Male

OBJECTIVETo investigate the difference in the CD4+T lymphocytes activation between long term non progressors (LTNP) and typical progressors (TP) of HIV-1 infected patients.

METHODSTwenty-four HIV-1 infected patients and 15 heathy control adults were tested and flow cytometry was used to detect the activation marker CD38 and CD4 count in blood samples taken from the patients and control. bDNA method was used to test the virus load in the plasma of patients.

RESULTSThe activation of CD4+T cells was positively correlated with virus load and negatively correlated with CD4 counts. Compared with normal controls, the activation of CD4+T cells was obviously increased in TP patients but not obviously changed in LTNP patients.

CONCLUSIONCompared with healthy controls, the activation of CD4+T cells in LTNP did not obviously increase. This maybe partially accounts for LTNP patients keeping a good state for a long time.

ADP-ribosyl Cyclase 1 ; analysis ; Adult ; CD4 Lymphocyte Count ; CD4-Positive T-Lymphocytes ; cytology ; immunology ; Female ; Flow Cytometry ; HIV Infections ; blood ; immunology ; virology ; HIV-1 ; genetics ; physiology ; Host-Pathogen Interactions ; Humans ; Lymphocyte Activation ; immunology ; Male ; Middle Aged ; RNA, Viral ; blood ; Time Factors ; Viral Load ; Young Adult

Objective To explore the risk factors for measles among children under 7 years old in Wenzhou, and to provideevidence for establishing scientific strategies on measles elimination. Methods A case-control study was carried out usingmeasles cases(age <7) reported between 2013 to 2015 from the Wenzhou Measles Surveillance System (WZMSS) . Asample of 198 cases were generated from the WZMSS confirmed cases of measles, and 371 controls were generated from theWZMSS excluded cases of measles. General characteristics and potential risk factors were collected, such as sex, age,original place of residence, length of stay in Wenzhou, history of hospital exposure and measles immunization history(i.e.receiving measles-containing vaccine) and so on. An univariate and multivariate logistic regression models were used toassess the association between different factors and the incidence of measles , and to investigate the risk factors that influencethe incidence of measles. Results A total of 198 measles cases among children under age 7 were reported between 2013 to2015 in Wenzhou, taking up 67.58% of the total reported measles cases of WZMSS, and suggesting an average of annualincidence rate of 8.85/10 million. The incidence ratio of male to female was 1.57:1.00. Children of 6-8 months old had thehighest incidence rate of 151.66/10 million. The incidence rate among migrant children was 15.01/10 million and wassignificantly higher thanlocal children(P<0.05) . Univariate logistic regression showed that the incidence of measles weresignificantly associated with age, original place of residence, length of stay in Wenzhou, history of hospital exposure andmeasles immunization history(P<0.05) . Multivariate logistic regression showed that migrant children(OR =2.28, 95%CI:1.56-3.33), no measles immunization history(OR=3.83, 95%CI: 2.48-5.92) and having hospital exposure(OR =2.35, 95%CI: 1.58-3.47) were risk factors for the incidence of measles. Conclusion Children of 6-8 months old had thehighest incidence rate of measles. Migrant children, nomeasles immunization history and having hospital exposurecould increase the incidence rate of measles among children younger than 7.