1.MEDIUM OPTIMIZATION AND INOCULANT TYPE COMPARISON OF RHIZOBIUM
Microbiology 1992;0(02):-
room temperature.
2.Effects of neural stem cell transplantation on hippocampus synaptophysin expression and learning memory abilities of Alzheimer disease rats
Chun YANG ; Hui ZHOU ; Linlin BAI ; Shuchun WANG ; Qian ZHANG
Chinese Journal of Tissue Engineering Research 2010;14(10):1803-1807
BACKGROUND:Previous studies have demonstrated that transplanted neural stem cells can survive and proliferate in the brain of Alzheimer disease(AD)rats,however,it is poorly understood whether it can rebuild the nerve tracts by substituting the injured or dead neurons and improve learning and memory abilities.Synaptophysin is one of the important markers of synaptic rebuilding.OBJECTIVE:To observe the effects of neural stem cell transplantation on synaptophysin expression in hippocampus and learning and memory abilities of AD rats.METHODS:Sprague Dawley rats were randomly divided into the normal control,AD model,2-week-transplantation and 4-week-transplantation groups.All rats were established AD models except that in the normal control group.Neural stem cells were isolated from the dentate gyrus of hippocampus of newborn rats,labeled with Hoechst33258,and then transplanted into CA1 region of hippocampus of rats in the 2-week-transplantation and 4-week-transplantation groups.The behavioral testing in the rats was performed using Y-maze trial.Nissl staining and synaptophysin immunohistochemistry were detected after the rats were sacrificed.The same volume of stroke-physiological saline solution was injected into rats in the AD models group using the identical methods.There was no treatment in the normal control group.RESULTS AND CONCLUSION:①The cells number in the hippocampal CA1 region of the 2-week-transplantation and 4-week-transplantation groups were increased than that of AD model group,but were still less than that of the normal control group(P < 0.05).There was no significantly difference between the absorbance values of 2-or 4-week-transplantation group and control group(P > 0.05).②The absorbance values of the 2-week-transplantation and 4-week-transplantation were significantly greater than that of the control and AD model groups(P < 0.05).③The learning and memory abilities in 2-and 4-week-transplantation group enhanced obviously and their correct reaction rates improved evidently,which was found statistically significant difference from AD model group(P < 0.05),while no statistically significant difference from control group(P > 0.05).The transplanted neural stem cells may promote the synaptic rebuilding and improve learning and memory abilities in AD rats.
3.Effects of Niupo Zhibao Pellet on high-mobility group box-1 protein expression in lung tissues of endotoxin shock rats.
Hui LI ; Shaohui DU ; Saixia ZHANG ; Rudong DENG ; Chun LI ; Yiwei LI ; Dongfeng CHEN ; Jianhong ZHOU
Journal of Integrative Medicine 2009;7(5):441-6
To observe the effects of Niupo Zhibao Pellet, a compound traditional Chinese herbal medicine, on high-mobility group box-1 protein (HMGB1) expression in lung tissues of rats with endotoxin shock.
4.The structural study of prefabrication stress stent and the hemodynamics in percutaneous transhepatic portacaval shunt
Jian-Guo CHU ; Xiao-Li SUN ; Yi-Jun ZHOU ; He HUANG ; Hua ZHOU ; Chun-Yan LV ; Shu-Hui YANG ;
Journal of Interventional Radiology 2006;0(11):-
Objective To present a preliminary latest procedure for portal hypertension and evaluate the technical feasibility and efficacy of portacaval shunt creation through the percutaneous transhepatic approach in order to make a hemodynamic comparison with that of the classic TIPS.Methods Thirty-eight patients with portal hypertension(36 men;mean age 57 years,range 32~73)were referred for PTPS procedure because of bleeding varices(n=36),intractable ascites(n=1),and hepatopulmonary syndrome(n=1).The severity of liver disease was classified as Child-Pugh B in 27 and C in 11.The PTPS was created by a percutaneous transhepatic puncture into right portal vein and then through left portal vein to the hepatic segment of IVC followed by a prefabrication stress stent-graft placement at the very site.Results Technical and functional success of 100% was achieved in all patients,without related complications.The postprocedural portal vein-IVC gradients mean 13 cmH_2O was achieved with the follow-up period mean 493 days.No recurrence of variceal bleeding and controlled refractory ascites were achieved,and still more with primary patency rate of the involved vascular structure up to 94.8% at 365 days,much better than classic TIPS. Conclusions Portacaval shunt creation using the prefabrication stress stent via percutaneous transhepatic technique is safe and feasible.The compact coincidence was obtained between the stent and the involved vessel with restoration of intrahepatic portal venous bemodynamics together with partial lowering of portal venous pressure and guaranteeing intrahepatic perfusion through right portal vein.It is also obviously to have postoperative prevention of shunt restenoses and lowering postoperative incidence of hepato-encephalopathy.
5.Mutation frequency analysis of mitochondrial ND1 gene associated with Leber hereditary optic neuropathy in Chinese population
Fu-xin, ZHAO ; Xiang-tian, ZHOU ; Juan-juan, ZHANG ; Jia, QU ; Yan-chun, JI ; Yu, ZHANG ; Hui-hui, ZHOU ; Xian-ning, DAI ; Min-xin, GUAN
Chinese Journal of Experimental Ophthalmology 2012;30(8):753-756
Background Leber hereditary optic neuropathy (LHON)is a common inherited eye disease,which generally affects young adults with bilateral loss of central vision.Mutation frequency of Leber hereditary has not been fully clarified. Objective This study was to investigate the mutation frequency of mitochondrial NDI gene associated with LHON in Chinese population. Methods The proposal of the study was approved by Ethic Committee of Wenzhou Medical College.Written informed consent was obtained from each subject initial of this trial.Eight hundred and ninety-four LHON patients and 134 normal subjects were collected.Genomic DNA was extracted from peripheral blood leukocytes of the all participants.Polymerase chain reaction (PCR) was used to amplify and sequence analysis of the mitochondrial ND1 gene was performed and aligned with revised Cambridge Reference Sequence(rCRS) of mitochondrial DNA.Then mutated gene frequency was screened and analyzed. Results Mutational analysis of mitochondrial ND1 gene in 894 LHON patients revealed the presence of G3316A,T3394C,G3460A,C3497T,G3635A,G3733A,and T4216C.11.19% LHON patients (100/894 ) were found to be associated with the gene mutations mentioned above,and 3.24% patients (29/894) showed the co-occurrence of three primary mutations.Mutation frequencies in LHON patients were 2.57%,2.23%,1.45%,3.80%,0.67%,0.11%,0.34%,respectively,and G3316A,T3394C,C3497T and T4216C also were detected in 134 normal controls with the mutation frequencies of 4.48%,2.99%,4.48% and 1.49%,respectively.Mutation frequency analysis showed an insignificant difference in the mutations of G3316A,T3394C,C3497T and T4216C between LHON patients and normal controls (x2 =0.926,P=0.336;x2 =0.052,P=0.820; x2 =0.142,P=0.707;P=0.129).G3376A,G3496T,G3700A,A4136G,T4160C and C4171A were absent in Chinese LHON patients. Conclusions Mitoehondrial ND1 gene in LHON is a mutational hotspot in Chinese population,11.19% (100/894)associated with LHON was caused by ND1 gene mutation.G3635A,G3733A may be rare pathological mutation in Chinese population.However,G3316A,T3394C,C3497T and T4216C are insufficient to produce the clinical phenotype,but they may play a synergic role for penetrance and phenotypic manifestation in LHON.
6.Effect of the compound of poly lactic-co-glycolic acid and bone marrow stromal cells modified by osteoprotegerin gene on the periodontal regeneration in Beagle dog periodontal defects.
Wei ZHOU ; Chun-hui ZHAO ; Ling-xuan MEI
West China Journal of Stomatology 2010;28(3):324-329
OBJECTIVETo evaluate the effect of the osteoprotegerin (OPG) gene-modified autologous bone marrow stromal cells (BMSCs) on regeneration of periodontal defects, and to provide new experimental evidence to explore the gene therapy for periodontal disease.
METHODSpSecTag2/B-opg was transduced into BMSCs by lipofectamine 2000. The expression of OPG protein in the BMSCs was detected by immunocytochemistry and Western blot. Inverted phase contrast microscope and scanning electron microscopy (SEM) were used to observe the morphology and proliferation of the BMSCs(OPG) on on the surface of the poly lactic-co-glycolic (PLGA). Horizontal alveolar bone defect (4 mmx4 mmx 3 mm) were surgically created in the buccal aspect of the mandibular premolar, and were randomly assigned to receive BMSCs(OPG)-PLGA (cells/material/OPG), BMSCs-PLGA (cells/material), PLGA (material), or root planning only (blank control). The animals were euthanized at 6 weeks post surgery for histological analysis. The height of new alveolar bone and cementum and the formation of new connective tissue were analyzed and compared. All data were statistically analyzed using the q test.
RESULTSThe BMSCs transfected by human OPG gene can highly express OPG protein. SEM observations demonstrated that BMSCs(OPG) were able to proliferate and massively colonize on the scaffolds structure. After 6 weeks, the height of new alveolar bone and cementum and the formation of new connective tissue were significantly greater in the experimental group than in the control groups (P < 0.05).
CONCLUSIONBMSCs(OPG)-PLGA can significantly promote the regeneration of dog's periodontal bone defects. Gene therapy utilizing OPG may offer the potential for periodontal tissue engineering applications.
Animals ; Bone Regeneration ; Dental Cementum ; Dogs ; Glycolates ; Lactic Acid ; Mesenchymal Stromal Cells ; Osteoprotegerin ; Polyesters ; Polymers ; Regeneration ; Tissue Engineering
7.Active ingredients of Plastrum Testudinis inhibit epidermal stem cell apoptosis in serum-deprived culture.
Lan CHEN ; Hui LI ; Chun LI ; Jiahui CAO ; Yiling WU ; Yang LIU ; Jianhong ZHOU ; Dongfeng CHEN ; Yiwei LI
Journal of Integrative Medicine 2011;9(8):888-93
To investigate the effects of active ingredients of Plastrum Testudinis (PT) on serum deprivation-induced apoptosis of epidermal stem cells (ESCs).
8.Effect and mechanism of Guhong injection against cerebral ischemia reperfusion injury.
Ming-chun SHU ; Hai-tong WAN ; Hui-fen ZHOU ; Jie-hong YANG ; Tao ZHAO ; Wei FU ; Yu HE
China Journal of Chinese Materia Medica 2014;39(24):4829-4833
To explore the effect and mechanism of Guhong injection against cerebral ischemia reperfusion injury, SD rats were randomly divided into the sham-operated group, the model group, the nimodipine group, and high, medium and low-dose Guhong injection groups, with 10 rats in each group. The middle cerebral artery embolization (MCAO) model was established to observe neurological deficit symptoms, infarct volume, SOD activity, MDA content, GSH-Px and CAT activity in rats, as well as the contents of t-PA, PAI, TXB2 and 6-keto-PGF1α in serum. The results showed that Guhong injection could obviously promote the recovery of neurological deficit symptoms, narrow the brain infarct volume in rats after surgery, significantlyincrease the activities of SOD, GSH-Px and CAT and decrease the content of MDA. Meanwhile, it also could obviously increase the contents of t-PA and 6-keto-PGF1α and decrease the contents of PAI and TXB2 in serum, indicating that Guhong injection have better antioxidant and antithrombus effects, as well as a significant protective effect against cerebral ischemia reperfusion injury in rats.
Animals
;
Antioxidants
;
pharmacology
;
Brain Ischemia
;
drug therapy
;
Catalase
;
metabolism
;
Drugs, Chinese Herbal
;
pharmacology
;
Injections
;
Male
;
Malondialdehyde
;
metabolism
;
Random Allocation
;
Rats
;
Rats, Sprague-Dawley
;
Reperfusion Injury
;
drug therapy
;
Superoxide Dismutase
;
metabolism
9.Finite element analysis of the effects of implant thread locations on stress distribution.
Jing-hui ZHAO ; Yan-min ZHOU ; Chun-yan LI
West China Journal of Stomatology 2008;26(6):584-587
OBJECTIVETo investigate the effects of different implant thread locations on stress distribution in implant and bone under concentrated load, and discuss the best design of location of implant thread.
METHODSThe stress in implant-bone interface of implants with different thread locations (model A, B, C, D), which were loaded in axial and oblique 450 to axle, were analyzed by Solidworks 2005 plus and Cosmos/works 7.0 software.
RESULTSThe stresses in cervical cortical bone of implant with model C were lower than others. But under oblique load, the stresses of implant and cancellous bone in model C were higher than model A. The stresses distribution of model B was obviously concentrated, and under axial load, all kinds of stresses in this model were higher than others. The stress distribution in model A and D were more even, the stress concentrated more on implant neck, upper part of cortical bone and the lowest thread on implant. The stresses in interface under oblique load were much higher than those under axial load.
CONCLUSIONThe location of thread has effect on stress distribution in implant-bone interface. More attention should be paid to it when we design implants, and we should try to avoid oblique load in implant prosthodontics.
Dental Implants ; Dental Prosthesis Design ; Dental Stress Analysis ; Finite Element Analysis ; Humans ; Stress, Mechanical