1.Analysis on medication principles for cough based on experience of Xu Di-hua, descendant of Meng He Medical School.
Xiao-jing XU ; Li-min XU ; Chun-feng SHEN ; Cai-hua WANG ; Chun-ti SHEN
China Journal of Chinese Materia Medica 2015;40(21):4301-4305
Based on the software of traditional Chinese medicine inheritance support system (TCMISS), this article aims to analyze the experience and composition rules for cough from the descendant of Meng He Medical School, Xu Di-hua. The cough cases treated by Xu Di-hua were collected, and recorded into TCMISS (V2.0). Data mining methods such as Apriori algorithm and complex system entropy cluster were used to analyze the medication principles of Xu Di-hua for cough from pathogenesis and therapeutie aspects, and dig out the frequency of the herbs in prescription, core medicine and new combinations. The experience of curing cough from Professor Xu Di-hua were well found in the research. He is good at choosing prescriptions accurately, and pays attention to simultaneous use of cold and moisture drugs with combination of tonification and purgation. He is skilled in adding or reducing materia medica flexibly, as well as regulating lung to relieve cough and eliminating phlegm by clearing heat.
Algorithms
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Cough
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drug therapy
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Data Mining
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Drug Therapy, Combination
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Drugs, Chinese Herbal
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chemistry
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therapeutic use
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Female
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Humans
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Male
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Materia Medica
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Medicine, Chinese Traditional
2.Changes of Serum Zinc Level of Rotaviral Enteritis in Children and Therapeutic Effect of Zinc
chun-hua, KANG ; xiao-shan, QIU ; li, HUA ; huan-kui, HUANG
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To explore the change of serum zinc level of children with rotavirus(RV) enteritis and efficacy of zinc supplementation on children with rotaviral enteritis.Methods One thousand one hundred and eighty-three healthy children were enrolled in this study,who were divided into different groups,and the zinc levels of children in every different age group were detected.These levels were used as normal levels of children in Dongguan district.One hundred and forty-three diarrhea patients in this 2 years were enrolled.Confirmed dia-gnosis was depended on the RV test in patients′ stool.The children with diarrhea were randomly divided into zinc treatment group (n=72) and conventional therapy group (n=71).The serum zinc levels in RV enteritis was analyzed.The patients in conventional therapy group were received diarrhea conventional therapy inchuded smectite and fluid infusion and so on;in addition to diarrhea conventional therapy,zinc gluconate 20 mg/d (10 mg/d below 6 months of age) for successive 14 days were given the patientsin zinc treatment group.The different efficacy of the 2 groups and the changes of serum zinc in treatment group were analyzed.Results Compared with normal children,the ones suffering from RV enteritis had lower serum zinc level(P0.05).Conclusions The ones suffering from RV enteritis have lower serum zinc level.In addition to diarrhea conventional therapy,giving zinc treatment has better effect on enhancing curative effect of RV enteritis.On the other hand,the suggestion of zinc supplement made by WHO is also suitable for Chinese children.
3.Qualitative and quantitative detection of Poria cocos by near infrared reflectance spectroscopy.
Xiao-huan FU ; Jun-hua HU ; Jia-chun LI ; Yin-hua DING ; Zhen-zhong WANG ; Wei XIAO ; Zhen-qiu ZHANG
China Journal of Chinese Materia Medica 2015;40(2):280-286
OBJECTIVEThe present study is concerning qualitative and quantitative detection of Poria cocos quality based on FT-near infrared (FT-NIR) spectroscopy combined with chemometrics.
METHODThe Poria cocos polysaccharides contents were determined by UV. Transmission mode was used in the collection of NIR spectral samples. The pretreatment method was first derivation and vector normalization. Then principal component analysis (PCA) was used to build classification model and partial least square (PLS) to build the calibration model.
RESULTThe results showed that conventional criteria such as the R, root mean square error of calibration (RMSEC), and the root mean square error of prediction (RMSEP) are 0.944 0, 0.072 1 and 0.076 2, respectively. The misclassified sample is 0 using the qualitative model built by PCA.
CONCLUSIONThe prediction models based on NIR have a better performance with high precision, good stability and adaptability and can be used to predict the polysaccharose content of Poria cocos rapidly, which can provide a fast approach to discriminate the different parts of Poria cocos.
Fungal Polysaccharides ; analysis ; Least-Squares Analysis ; Poria ; chemistry ; Principal Component Analysis ; Spectroscopy, Near-Infrared ; methods
4.Optimization of electroporation parameters in HL-60 cells for STIM1 siRNA interference during its differentiation.
Hai-Yang CHEN ; Wen-Ying ZOU ; Cui-Hua XIE ; Xiao-Jing MENG ; Chun-Qing CAI
Chinese Journal of Applied Physiology 2011;27(4):497-499
Cell Transformation, Neoplastic
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drug effects
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genetics
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Dimethyl Sulfoxide
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pharmacology
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Electroporation
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methods
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HL-60 Cells
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Humans
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Membrane Proteins
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genetics
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Neoplasm Proteins
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genetics
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RNA Interference
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RNA, Small Interfering
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genetics
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Stromal Interaction Molecule 1
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Transfection
5.Research on pericellular matrix properties for chondrcytes.
Jun-liang HAN ; Wang-ping DUAN ; Guang-hua SHI ; Wei YUAN ; Xiao-chun WEI
China Journal of Orthopaedics and Traumatology 2015;28(6):576-579
Pericellular matrix (PCM) is a narrow tissue region surrounding chondrocytes, which "chondron" with its enclosed cells. A number of studies suggested that PCM is rich in proteoglycans, collagen and fibronectin, and plays an important role in regulating microenvironment of chondrocytes. Direct measures of PCM properties through micropipette aspiration technique showed that PCM was different from mechanical property of chondrocytes and nature extracellular matrix. However, the function of PCM is not clear, and need further study.
Animals
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Biomechanical Phenomena
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Chondrocytes
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chemistry
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cytology
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metabolism
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Extracellular Matrix
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chemistry
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metabolism
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Humans
6.Study on HPLC fingerprint of jinzhen oral solution.
Jun-Hua HU ; Qian-Qian SU ; Ting-Ting LI ; Jia-Chun LI ; Wei XIAO
China Journal of Chinese Materia Medica 2014;39(19):3768-3771
The HPLC fingerprint determination method of Jinzhen oral solution was established to provide a new method for quality control of Jinzhen oral solution. RP-HPLC was used for phenomenex Luna C18 (4.6 mm x 250 mm, 5 μm) chromatographic column, with 0.1% H3 PO4 water solution and acetonitrile as the mobile phase for gradient elution. The detection wavelength was 280 nm. HPLC fingerprint of Jinzhen oral solution was established to identify 17 common peaks in Jinzhen oral solution. The similarity of fingerprints of 10 batches of finished products was more than 0. 90. The established HPLC fingerprint has a better precision, reproducibility and stability, and can be applied in quality control of Jinzhen oral solution.
Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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chemistry
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Quality Control
7.Effect comparison of Wright-Giemsa stain under different conditions and its apply in cell micronucleus test.
Chun-hua LIAO ; Huan-feng BIAN ; Fang XIAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(2):139-141
OBJECTIVETo apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times.
METHODSUse Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (3:1-1:3) and different staining times (1, 3, 5, 10, 15 min).
RESULTSAfter stained for 3-5 min with the proportion ratio of 3:1 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa.
CONCLUSIONSWright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.
Azure Stains ; Coloring Agents ; Hep G2 Cells ; Humans ; Micronucleus Tests ; Staining and Labeling ; methods
8.Tongxinluo enhancing proliferation of peripheral blood-derived human endothelial progenitor cells
Xiao-Wei LIANG ; Cheng-Bo SUN ; Hua WANG ; Chun LIANG ; Zong-Gui WU ;
Academic Journal of Second Military Medical University 1985;0(06):-
Objective:To study the effects of Tongxinluo,a traditional Chinese medicine,on the proliferation of peripheral blood-derived endothelial progenitor cells(EPCs)in vitro.Methods:The Tongxinluo solution was prepared through ultrasonication according to the pervious literature.The mononuclear cells(MNCs)were isolated from the peripheral blood with Ficoll by density gradient centrifugation.MNCs were suspended in Medium 199 containing 20% fetal blood serum(FBS)and vascular endothelial growth factor(VEGF).After cultured for 7 d,the attached cells were characterized by Di-LDL uptaking and FITC-lectin binding by laser confocal microscope,and further identified through detection of CD34 and CD133 expression by flow cytometry.Then the cultured EPCs were incubated with Tongxinluo at a series of concentrations(0,50,100,200,500, 750,1000?g/ml)for different durations(0,6,12,24 and 36 h).The cell morphology was observed and cell proliferation was determined by MTT assay.Results:Incubation with different concentrations of Tongxinluo increased the proliferative ability of EPCs.Tongxinluo at 500?g/ml had the most prominent effect on proliferation and the effect increased as time went by and reached peak at 36 h(growth rate 54.18%,P
9.A clinical study on coadministration of Cyclosporin A and berberin hydrochloride in renal transplanted recipients
Ai-Rong YU ; Hua-Wen XIN ; Xiao-Chun WU ; Qing LI ;
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(12):-
AIM:To study the effect of berberin hydrochloride(Ber)on the elevation of blood concentra- tion of Cyclosporin A(CsA)and liver and renal functions in renal transplanted recipients.METHODS:138 cases with renal transplantation in experimental group were treated with CsA and Ber and 150 cases with renal trans- plantation in control group received CsA only.Blood lev- els of CsA,biochemistry indexes for liver and renal func- tion were determined.RESULTS:After 1,3,6 months combined with Ber,blood concentrations of CsA in pa- tients went up by 49.8%,81.4%,36.9% and the radios of blood concentration of CsA to CsA dosage increased 88.8%,102.0%,72.5% compared with those before taking Ber(P
10.The Microorganism Constitutes Analysis of Soy Sauce Liquor from High-Salt-Level Watery State Fermentation
Xiao-Bao XIE ; You-Sheng OUYANG ; Hai-Yan ZENG ; Chun-Hua WANG ; Yi-Ben CHEN ;
Microbiology 1992;0(03):-
A system analysis about the microbial flora of normal and abnormal soybean sauce liquor from the high-salt-level watery state fermentation was made and the dominant bacteria and yeasts were identified.On the other hand,a discussion of effect of temperature on microbial flora was made.The results indicated that there were no obvious differences about the count of aerobe,spore-producing bacteria,enterobacteriaceae,lactic acid bacteria and anaerobe between the normal and abnormal soybean sauce liquor and there were marked differences about the count of yeasts and salt-tolerant bacteria.The predominant yeasts in normal soybean sauce were Torulopsis and Saccharomyces,accounting for 55.9% and 35.3% of the total yeasts separately,and in abnormal soybean sauce were Pichia,candida and Saccharomyces,accounting for 62.8%,17.9% and 9.0% respectively.