Abstract?AIM: To discuss clinical efficacy and safety of 10g/L atropine with short covering for children with amblyopia.?METHODS: Eighty -eight children ( 88 eyes ) with amblyopia, staying in hospital from February 2011 to February 2014 for treatment, were divided into control group ( n =44 ) and observation group ( n =44 ) . The control group only given short covering therapy was observed.Observation group was given 10g/L atropine treatment besides covering.Clinical efficacy, treatment compliance, visual acuity, corrected spherical degree of amblyopia eye and adverse events were observed and compared.?RESULTS:1) After treatment, total effective rate of the observation group was 95% ( 42/44 ) , significantly higher than that of control group ( 80%, 35/44, P<0.05 ); 2 ) excellent compliance rate of the observation group was 95% ( 42/44 ) , significantly higher than that of control group (82%, 36/44, P<0.05);3) visual acuity of the two groups when the disease was first diagnosed was not significantly different (P>0.05), but increased number of lines of vision and corrected spherical degree of amblyopia eye in the observation group were significantly higher (P<0.05);4) in the observation group total rate of adverse events was 9% ( 4/44 ) , significantly lower than that in the control group (23%, 10/44, P<0.05).?CONCLUSION: The combined therapy, 1% atropine with short covering, is effective and safe for amblyopia in children.

Objective To investigate the inhibitory effect of paclitaxel on rat graft arteriosclero- sis and the mechanism.Methods The rat abdominal aortic allograft model was used.All rats were divided into three groups:isograft control group (Wistar to Wistar),allograft group (Wistar to SD) and allograft paclitaxel-treated group (Wistar to SD).Rats in allograft paclitaxel-treated group re- ceived paclitaxel (2 mg?kg~(-1)?d~(-1)) from the operation day to post-operative day 14 and others received same dosage of vehicle (0.9% normal saline).Animals were sacrificed and the grafts were harvested at 30th day after operation.Intimal proliferation was studied by light microscopy.The apoptosis of vascular smooth muscle cells (VSMCs) was detected by transmission electronic microscopy and termi- nal deoxynucleotidyl transferase biotin nick end-labeling (TUNEL) method.Results Morphological analysis showed that grafts had no change after operation in isograft control group,but in allograft group intimal proliferation,inflammatory cells infiltration in neointima and adventitia and stenosis of allografts were obvious.After treatment with paclitaxel,there was a significant decrease in intimal proliferation,inflammatory cells infiltration and stenosis.Apoptosis index of VSMCs was higher in the allograft paclitaxel-treated group than other groups.Conclusion Paclitaxel can inhibit intimal pro- liferation in aortic allografts and prevent the graft from arteriosclerosis possibly by inducing the apoptosis of VSMCs.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Fracture Healing ; drug effects ; Humans ; Male ; Middle Aged ; Phytotherapy ; Tibial Fractures ; drug therapy

Objective To investigate the influence of thiamine deficiency(TD)at early pre- pathological lesion stage on cognitive function and the correlation between cognitive dysfunction and hippocampal neurogenesis.Methods TD mouse model was prepared by feeding a thiamine-depleted diet. Learning and memory functions of TD mice were tested with Y-maze.Hippoeampal neurogenesis was studied with bromodeoxyuridine(BrdU),proliferative cell nuclear antigen(PCNA),and Doublecortin(Dcx) immunohistochemical staining on the 7th(TD7),9th,14th and TD25th day.Results TD9 mice without pathological impairment and cholinergic nerve degeneration needed more times of training(22.3?2.2)in the learning test of Y maze compared with the controls(13.5?3.5).Correspondingly,the numbers of BrdU-positive ceils and the immunoreactivity of Dcx decreased significantly in the TD9 mice(19.8?0.4, 1537.2?50.2 vs 23.9?0.3,2688.9?127.9 pixels/mm~2).Thiamine re-administration reversed the declined hippocampal neurogenesis:the number of BrdU-positive cells was 23.6?1.9 and Dcx immunoreactivity was 2052.3?269.6 pixels/mm~2:the impaired learning ability was simultaneously restored,with the number of total training trial being 16.8?0.5.Conclusion The decreased hippocampal neurogenesis contributes to retarded learning ability at early pre-pathological lesion stage of TD.

Objective To investigate the palatal bone thickness in adult with normal occlusion Methods The cone beam computerized tomography records of 32 adults with normal occlusion ( 16 males and 16 females), mean age (30. 1 ±6. 5) years, were used to measure the bone thickness at midpalatal area and the right and left palatal sides. Coronal slices at 3 mm intervals were generated. Slice 1 was the coronal slice through the posterior border to the incisive foramen, while Slice 7 was the coronal slice 18 mm away from the incisive foramen. At each coronal slice, the midpalatal sites were Site M and the sites on the exterior margin of the hard palatal were Site D. Four equally divided parts on the line linking Site M to Site D were named Site A, B, C from the interior to the exterior respectively. Palatal bone thickness were measured at these sites. Results Significant differences were noted from Slice 1 to Slice 7, the bone thickness of palate tended to decrease from the front to the back. The thickest site at hard palatal was 12. 6 mm, locating at Site D of Slice 1, while the thinnest site was 2. 7 mm, locating at Site B of Slice 7.The palatal bone thickness ranged from 10. 5 mm(maximum) to 5. 8 mm(minimum) at Slice 2 and Slice 3. No statistical significance was found between the left and right sides ( P ＞ 0. 05 ). Conclusions The favorable sites for miniscrew placement were the anterior region of the hard palate in adult. The length of miniscrew ranged from 5 mm to 10 mm can be placed from 6 mm posterior to the incisive foramen.

This study aimed to analyze the etiology of the encephalitis outbreak in Longyan, Fujian Province, China in 2010, in order to provide valuable information for this prevention and control of this disease. Pathogens were confirmed from cerebrospinal fluid samples with fluorescent RT-PCR, virus isolation (RD cells), and neutralization tests. Then, the VP1 fragments or whole genome nucleotide sequences were determined for four virus strains using PCR. Homology was assessed using the MegAlign software, and a phylogenetic evolutionary tree was drawn using Mega 4.0 software. The results confirmed that the etiology of the outbreak was the ECHO6 intestinal virus, and the nucleotide sequence of the VP1 segment indicated that the C2 subtype was responsible. The genome sequence consisted of 7407 nucleotides, and resembled the genome of other ECHO and CoxB viruses with homology levels of 78.5%-87.3%. The encephalitis outbreak in Longyan in 2010 was caused by the ECHO6 C2 subtype intestinal virus, and its complete genome sequence length is similar to the standard strain (U16283) with a sequence homology of 80.4%.
Child, Preschool ; China ; epidemiology ; Disease Outbreaks ; Echovirus 6, Human ; classification ; genetics ; isolation & purification ; Echovirus Infections ; epidemiology ; virology ; Encephalitis ; epidemiology ; virology ; Female ; Humans ; Infant ; Male ; Molecular Sequence Data ; Phylogeny

Background Wearing of orthokeratology contact lens can slow down the development of myopia,especially in childhood.However,if wearing orthokeratology contact lens for long-term affect the corneal health is worthy of consideration.Objective This study was to evaluate the security of wearing orthokeratology contact lens in children.Methods A retrospective case-observational study was adopted.One hundred and fifty eyes of 76 myopic children aged 8-15 years were enrolled in Shanghai Tenth People's Hospital during December,2009 to December,2011.Orthokeratology contact lens were worn at night for 8-10 hours daily.Area of corneal endothelial cells,density of corneal endothelial cells,coefficient of variation of corneal endothelial cells and percentage of hexagonal cells were measured before wearing and 3,6,12,24 months after wearing.Repeated measurement of single factor analysis of variance was used to evaluate the changes of corneal endothelial cells after wearing of orthokeratology contact lens.Results No significant differences were found in the area of corneal endothelial cells and density of corneal endothelial cells among the different time points (F =11.34,P＞0.05 ; F =7.16,P＞0.05).A significant difference was seen in the coefficient of variation of corneal endothelial cells at various time points in the children(F=12.70,P=0.02),and the coefficient of variation of corneal endothelial cells was 0.36±0.02 24 months after wearing lens,which was higher than 0.28 ±0.05 before wearing lens (P＜0.05).In addition,the percentage of hexagonal cells in different time points was significantly different (F=13.40,P =0.04),and the percentages of hexagonal cells at 12 hours and 24 months after wearing lens were (62±12)％ and (60±14)％ respectively,which was significantly lower than (69±12)％ before wearing lens (P＜0.05).Conclusions Wearing of orthokeratology contact lens for over one year leads to the decrease of percentage of hexagonal cells and increase of coefficient of variation of corneal endothelial cells.

This paper introduces the designing concept of the ECG treadmill system and discusses the methods of its realization.
Amplifiers, Electronic ; Computer Simulation ; Computer Systems ; Coronary Disease ; diagnosis ; Electrocardiography ; instrumentation ; methods ; Equipment Design ; Exercise Test ; instrumentation ; methods ; Humans ; Microcomputers ; Signal Processing, Computer-Assisted ; Software

2', 3', 5'-Tri-O-acetyl-N6-(3-hydroxylaniline)adenosine (WS070117) is a derivative compound of natural product cordycepin. It has significant lipids regulating activity and low toxicity which has been proved by in vitro and in vivo experiments. In this study, 1H NMR-based metabolomics was used to investigate the dose-related effects of WS070117 on hyperlipidemia of high-fat-fed hamsters. The hyperlipidemic hamsters were administrated with six different doses of WS070117, including 3, 12, 50, 100, 200 and 400 mg x kg(-1) x d(-1). 1H NMR spectra of hamster serum were visually and statistically analyzed using two multivariate analyses: principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). As a result, WS070117-treated groups showed dose-related regulation of metabolites associated with lipid metabolism, choline metabolism and glucose metabolism. The dose of 3 mg x kg(-1) x d(-1) of WS070117 only exhibited a little lipids regulating activity. However, the doses of 12 and 50 mg x kg(-1) x d(-1) of WS070117 both regulated the contents of metabolites to reverse significantly toward normal levels. When the dose of WS070117 reached 100 mg x kg(-1) x d(-1), it was more effective than positive control drugs. The work suggested that NMR-based metabolomics might be a valuable approach to evaluate dose-related effects of lipids regulating compounds.
Adenosine ; analogs & derivatives ; pharmacology ; Animals ; Cricetinae ; Hyperlipidemias ; metabolism ; Least-Squares Analysis ; Lipid Metabolism ; drug effects ; Magnetic Resonance Spectroscopy ; Metabolomics ; Multivariate Analysis ; Principal Component Analysis

It was long believed that platelets are prematurely destroyed in the reticuloendothelial system by platelet autoantibodies in idiopathic thrombocytopenic purpura. However, humoral mechanisms cannot account for all observations made in this disorder, and it is increasingly evident that cellular mechanisms contribute to platelet destruction. In this review the tolerance of T cell, abnormality of T cell apoptosis, abnormal activation of T cells, T cell subtype and its function changes, and T cell-mediated cytotoxicity were summarized.
Apoptosis ; immunology ; Autoantibodies ; immunology ; Humans ; Immune Tolerance ; Immunity, Cellular ; immunology ; Purpura, Thrombocytopenic, Idiopathic ; immunology ; T-Lymphocyte Subsets ; immunology ; T-Lymphocytes ; immunology ; pathology ; T-Lymphocytes, Cytotoxic ; immunology ; T-Lymphocytes, Helper-Inducer ; immunology