1.Regulation of c-fos gene expression in hepatic stellate cells by transforming growth factor beta.
Ai-Min WANG ; Chun-Gai REN ; Zhi-Jie MA ; Ying XIANG ; Zhi-Wei ZHANG ; Zhi-Jian ZHU ; Bao-En WANG
Journal of Southern Medical University 2009;29(7):1408-1409
OBJECTIVETo investigate the effects of transforming growth factor beta (TGF ) on c-fos gene expression in hepatic stellate cells.
METHODSHepatic stellate cells (HSC-T6) were cultured in the medium containing different concentrations of TGF (0.2, 1, and 5 ng/ml), and cells were collected at different time points of incubation (8, 24, 48, and 72 h). The total RNA of the HSCs was isolated and c-fos gene expression level were measured by reverse transcription polymerase chain reaction.
RESULTSc-fos gene expression levels of HSCs cultured in the presence of low (0.2 ng/ml), moderate (1 ng/ml) and high (5 ng/ml) concentrations of TGF for 8, 24, 48 and 72 h were significantly greater than those of control group. The c-fos gene expression levels of HSCs increased gradually with the increment of TGF concentration, and significant differences in c-fos gene expression were found between the 3TGF groups.
CONCLUSIONTGF strongly up-regulates c-fos gene expression in hepatic stellate cells.
Animals ; Cells, Cultured ; Gene Expression ; drug effects ; Genes, fos ; Hepatic Stellate Cells ; drug effects ; Proto-Oncogene Proteins c-fos ; genetics ; metabolism ; Rats ; Transforming Growth Factor beta ; pharmacology
2.Effects of anticoagulation protein defect in maternal plasma on spontaneous abortion.
Chun-mei BAI ; Shui-qing MA ; Ming-ying GAI ; Lian-kai FAN ; Feng-yan REN ; Guang-sheng FAN
Chinese Medical Sciences Journal 2004;19(4):290-292
OBJECTIVETo investigate the mechanism of anticoagulation protein defect in the pathogenesis of unexplained recurrent miscarriage.
METHODSFifty-seven patients with a history of unexplained abortion were enrolled as the investigation group for tests of protein C, protein S, antithrombin III (AT-III), as well as activated protein C resistance (APC-R). The control group consisted of fifty healthy women with a history of normal pregnancy and delivery. Blood samples were obtained for, measuring serum activity of protein C, protein S, AT-III, and APC-R. Patients with positive APC-R were tested for factor V (FV) Leiden gene mutation by PCR-RFLP method.
RESULTSOf the 57 patients, 12 (21.1%), 1 (1.8%), and 5 (8.8%) cases were found with protein S, protein C, and AT-III deficiency respectively, and 13 (22.8%) cases with positive results of APC-R. Of the control group, no protein C or AT-III deficiency was ever found, whereas 2 (4.0%) volunteers were presented with protein S deficiency and 3 (6.0%) with positive results of APC-R. No FV Leiden gene mutation was identified in all the patients with positive APC-R results. Late spontaneous abortion cases had higher incidence of anticoagulation protein defect than the early cases.
CONCLUSIONAnticoagulation protein defect may play a role in the pathogenesis of fetal loss, especially for those occurring in late stage of pregnancy.
Abortion, Habitual ; blood ; etiology ; Activated Protein C Resistance ; blood ; complications ; genetics ; Adult ; Antithrombin III ; metabolism ; Antithrombin III Deficiency ; blood ; complications ; Factor V ; genetics ; Female ; Humans ; Point Mutation ; Protein C ; metabolism ; Protein C Deficiency ; blood ; complications ; Protein S ; metabolism ; Protein S Deficiency ; blood ; complications
3.Evaluation of triple anti-platelet therapy by modified thrombelastography in patients with acute coronary syndrome.
Yi-hong REN ; Ting-shu YANG ; Yu WANG ; Lu-yue GAI ; Hong-bin LIU ; Lian CHEN ; Hong-ye WANG ; Chun-ya WANG ; Xiu-li XU ; Jing JIN ; You-hong XIN ; Rong-bin LI ; Hai-yan LI ; Lin LIN ; Chun-xue LIU
Chinese Medical Journal 2008;121(9):850-852
Acute Coronary Syndrome
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drug therapy
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Angioplasty, Balloon, Coronary
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Aspirin
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administration & dosage
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Humans
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Platelet Aggregation Inhibitors
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administration & dosage
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Thrombelastography
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Ticlopidine
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administration & dosage
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analogs & derivatives
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Tyrosine
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administration & dosage
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analogs & derivatives
4.The usage of comprehensive geriatric assessment in elderly patients with acute myeloid leukemia: a multicenter, prospective study.
Qi WU ; Rong FU ; Ming Feng ZHAO ; Yi Gai MA ; Hao JIANG ; Liang ding HU ; Yu JING ; Hui LIU ; Li Ru WANG ; Li SU ; Yong Qing ZHANG ; Chun Lin ZHOU ; Yan ZHANG ; Han Yun REN ; Bin JIANG ; He Bing ZHOU ; Lin KANG ; Lu ZHANG ; Dao Bin ZHOU ; Jian LI
Chinese Journal of Hematology 2019;40(1):35-39
Objective: To evaluate the feasibility and potential value of comprehensive geriatric assessment (CGA) in elderly (≥60 years) patients with newly diagnosed acute myeloid leukemia (AML) in China. Methods: The CGA results of 83 newly diagnosed AML (non-APL) patients from 16 hospitals in Beijing and Tianjin between March 2016 and December 2017 were prospectively collected and analyzed. The clinical data, treatment and follow-up information were also collected. Results: Of 83 newly diagnosed elderly AML patients, 81 patients (97.6%) completed all designated CGA assessment. The median number of impaired scales of the CGA assessment in the studied population was 2(0-6). Sixteen patients (19.3%) showed no impairments according to the geriatric assessment scales implem ented by this study. The distributions of impaired scales were as follows: impairment in ADL, 55.4%; IADL impairment, 42.2%; MNA-SF impairment, 48.2%; cognitive impairment, 15.7%; GDS impairment, 31.7%; HCT-CI impairment, 19.5%, respectively. In patients with "good" ECOG (n=46), the proportion of impairment for each CGA scale ranged from 6.5% to 37.0% and 32 patients (68.9%) had at least one impaired CGA scale. Survival analysis showed that the number of impaired scales of the CGA was significantly correlated with median overall survival (P=0.050). Conclusions: CGA was a tool with feasibility for the comprehensive evaluation in elderly AML patients in China. Combined with age and ECOG, CGA may be more comprehensive in assessing patients' physical condition.
Activities of Daily Living
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Aged
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China
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Geriatric Assessment
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Humans
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Leukemia, Myeloid, Acute
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Prospective Studies