Age Factors ; Aging ; immunology ; physiology ; Animals ; Lymphocyte Activation ; immunology ; Male ; Physical Conditioning, Animal ; physiology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Swimming ; physiology ; Thymus Gland ; immunology

Objective To analyze the changes of energy metabolism-related proteins(enzymes) and transformation of energy metabolism in transplanted heart.Methods The hearts transplanted from Lewis to Wister rats as allografts and from Lewis to Lewis rats as isografts were used to estab- lish Ono model.The changes in proteins were observed by using proteomics technique and compared between allografts and isografts in the left ventricular myocardial tissues 2 and 8 weeks after heart transplantation.Peptide mass fingerprint map was obtained by using matrix-assisted laser desorption/ ionization time of flight mass spectrometry(MALDI-TOF-MS)and the matched proteins achieved by using Matrix Science software.Results Eighty proteins(enzymes)of total 65 differential proteins that regulated energy metabolism were identified in allografted hearts,which correlated to the Krebs cycle and electron transport system involving glycometabolism,fatty acid and branched-chain amino acids oxidation.Conclusion The energy metabolism in allografts heart was transformed:mitochondria function was decreased in Krebs cycle but enhanced in electron transport system and outer mitoehon- drial membrane permeability to ATP,accelerated in glycolysis and fatty acid oxidation,however, branched-chain amino acids oxidation were suppressed.

Air Pollutants, Occupational ; Humans ; Occupational Exposure ; Styrene

Animals ; Cold-Shock Response ; Interleukin-2 ; blood ; Male ; Motor Activity ; Rats ; Rats, Sprague-Dawley ; beta-Endorphin ; blood

Objective To observe the changes of neural stem cells(NSCs) in subventricular in developing human fetal brain at(diffe)-rent ages.Methods Thirty cases of embryoes at gestational age 24-28 weeks induced by labor with water bag were collected to determin distribution,shapes and growth modes in subventricular with hybridization in situ under light microscope.Results NSCs expressing Nestin mRNA existed in subventricular from human fetal brain at different ages,NSCs existed in subventricular of different fetal age included astro-NSCs,each had enations from 3 to 6,and all projections crowded together into a reticular plexiform,in which NSCs districuted.Nucli were round in shape,each had nucleoli from 1 to 4.NSCs had rarefaction chromatin,most NSCs existed in a single growth mode,symmetral cleavage growth mode and clony with 3 NSCs would be seen.There were no differences between positive Nestin mRNA NSCs in distribution and cell shapes,but had differences in growth mode.Conclusion NSCs exist in subventricular from different gestational ages and their growth mode are changing with difference of gestational age.

Objective To explore a precise and dependable method for determining neural stem cells(NSCs)in hippocampus from human fetal brain.Methods Ten cases of embryo at gestational age 32 weeks and induction of labor with water bag were collected,affused and sliced including frost slice and paraffin slice for determining alteration of tissue and NSCs in hippocampus from human fetal brain with HE stain and immunohistochemical staining under light microscope.Results Nestin proteins located in NSCs and the number of NSCs were less in paraffin slice of tissue than those in frost slice.Integrality of structure of tissues and cells in paraffin slice excelled in frost slice.Conclusions There is a precise and dependable method including induction of labor with water bag and allusion and frost slice,which is necessary for wide useness in study of NSCs in human fetal brain.

Cerebral Hemorrhage ; epidemiology ; etiology ; prevention & control ; Cerebral Ventricles ; Humans ; Incidence ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; epidemiology ; etiology ; prevention & control

Nurses are the highest numbered healthcare professionals who work in a knowledgedriven environment, where accurate and updated information is needed when delivering care to clients. Information literacy has therefore become one of the criteria in determining nurses’ readiness for evidencebased practice in recent years. In the actual day-to-day care practice, are nurses ready for this? Objective: To determine the information literacy competency in readiness for evidence-based practice among clinical practicing registered nurses in a private hospital in Penang, Malaysia
Evidence-Based Practice ; Nurses ; Education, Nursing

A bacterium that contains a chitin deacetylase has been isolated from ocean soil. It has been identified Baciluus .The best suitable condition of producing this enzyme is that pH is 4.0,35℃,and the incubate time is 80 hours in the present of Ca 2+ as well as having no chitosan. The best temperature of this enzyme when it acts is 40℃～50℃ and the best pH range is 4.5～5.0.