Objective To study the implication of phosphoinositide-specific phospholipase C?2(PLC-?2) in gastric cancer MGC80-3 cells treated by TPA.Methods The effect of 12-O-tetradecanoylphorbol-1,3-acetate(TPA) on gastric cancer MGC80-3 cells was detected and analyzed by DAPI staining under fluorescence microscope.The effect of TPA on the expression level of PLC-?2 protein was detected by Western blotting,when nuclear and cytoplasmic protein fractions were prepared through lysis of cell and centrifugation.Localization and translocation of PLC-?2 were observed under laser scanning confocal microscope with immune-fluorescence technique.After MGC80-3 cells were pretreated by U73122(PLC-?2 inhibitor),the effect of TPA on PLC-?2 was detected by Western blotting and laser scanning confocal microscope with immunefluorescence technique,and the effect of U73122 on MGC80-3 cells followed by TPA was observed and analyzed by DAPI staining under fluorescence microscope. Results TPA induced the apoptosis of gastric cancer MGC80-3 cells.Meanwhile,TPA increased the expression level of PLC-?2 protein and induced its translocation from cytoplasm to nucleus.U73122 inhibited the effect of TPA on the expression level of PLC-?2 protein,but it did not affect on the cell apoptosis induced by TPA.However,the translocation of PLC-?2 protein induced by TPA was not inhibited by its inhibitor(U73122).Conclusion Although TPA could increase the expression level of PLC-?2 protein,it is not directly related to the cell apoptosis induced by TPA.However,its translocation might be related with the apoptosis of gastric cancer.

The co-metabolic carbon sources,dynamics and influence factors of atrazine biotransformation by genetically engineered microor- ganism(GEM)were investigated in the paper.The results showed that glucose was superior to acetate as co-metabolic carbon sources.The concentration of carbon sources affected little on atrazine transformation but much on GEM growth.The specific transformation rate(STR)of atrazine was influenced not by cell density of GEM but by atrazine concentration.Monod model was used to simulate atrazine transformation dynamics and the parameters were v_(max)=0.168/h and Ks=30.49 mg/L.When temperature reduced,STR decreased markedly.The trans- formation was efficient in weak alkaline condition and was inhibited in acidic condition.To some extent,salinity did not affect transformation activity of GEM.Co~(2+),Fe~(2+),Fe~(3+),and Zn~(2+) improved atrazine transformation,but Mn~(2+),Ni~(2+) and Cu~(2+) inhibited atrazine transforma- tion.The results also showed that there existed a direct correlation between adsorption and transformation of atrazine by GEM.

Brain Death ; diagnosis ; Breath Tests ; Cerebrovascular Circulation ; Child ; Child, Preschool ; Coma ; diagnosis ; Electroencephalography ; Humans ; Infant ; Infant, Newborn ; Neurologic Examination ; Practice Guidelines as Topic

Hepatic ischemia reperfusion injury (IRI) is a critical problem of liver surgery, especially when comes to liver transplantation. Presently, there are no effective measures for diagnosis, prevention and therapy of IRI, as the mechanisms of IRI still remain unclear. This review summarizes several new hepatic ischemia-reperfusion markers related to cell signal transduction pathway, including transcription factor STAT, HIF-1 and PPARs, transmission factor MAPK, membrane receptor TLR4 and PARs, and iNOS. Animal studies have indicated that IRI was ameliorated by activating or blockading these markers, which might serve as targets for diagnosis, prevention and therapy of IRI.

Objective: To observe the expression of local angiotensin-converting enzyme 2 (ACE-2) in rat liver and its relationship with preservation injury (PI) after transplantation, so as to determine the possible role of local renin-angiotensin system (RAS) in PI caused by the cold-preservation in liver transplantation. Methods: The donor liver grafts were divided into cold preservation (CP) and non-cold preservation (NCP) groups; we also included a sham operation group without transplantation. The mRNA and protein levels of ACE-2 in the transplanted livers were detected by quantitative real-time PCR and Western blotting assay, respectively. The location of ACE-2 protein and pimonidazole in liver was detected by immunohistochemistry. Furthermore, histological evaluation was applied to verify the extent of liver damage. Results: Histological injuries of different degrees existed after liver transplantation; the injury was more obvious in the CP group. Compared with the sham operation group, the mRNA and protein expression of ACE-2 was significantly increased after liver transplantation (P<0.05 or P<0.01), and the expression of the CP group was significantly higher than that of the NCP group (P<0.05). Pimonidazole staining was positive around the hepatic veins in the transplantation groups, and the intensity of staining was positively correlated with the expression of ACE-2 mRNA (r=0. 78, P<0.001). Conclusion: It is indicated that ACE-2 expression is closely associated with tissue hypoxia during cold preservation-induced PI, and local RAS may play an important role in PI during liver transplantation.

Humans ; Osteogenesis Imperfecta/diagnosis*

Antigens, Nuclear ; metabolism ; Cordotomy ; methods ; Diagnosis, Differential ; Ependymoma ; metabolism ; pathology ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Nerve Tissue Proteins ; metabolism ; Neurocytoma ; metabolism ; pathology ; surgery ; Oligodendroglioma ; S100 Proteins ; metabolism ; Spinal Cord Neoplasms ; metabolism ; pathology ; surgery ; Synaptophysin ; metabolism

Objective:To determine the di-allelic polymorphisms of TNF gene and their association with Helicobacter Pylori(H.pylori) infection and gastroduodenal diseases in Chinese population with Han nationality.Methods:Two hundreds and ten patients with gastroduodenal diseases(73 chronic gastritis,78 duodenal ulcer and 59 noncardia gastric cancer) and 264 healthy controls were genotyped by the PCR-RFLP method for TNF-? 308,lymphotoxin-?(LT-?) Nco Ⅰ and AspH Ⅰ gene polymorphisms.H.pylori infection status was determined by a validated serological test.Results:H.pylori infection was detected in 90.5% of 210 patients and 62.1% of 264 healthy controls(P

Adult ; Female ; Humans ; Middle Aged ; Posture ; Pregnancy ; Pregnancy Outcome ; Retrospective Studies ; Sedentary Lifestyle ; Surveys and Questionnaires ; Work ; Young Adult

This study was aimed to investigate active ingredients from A mp e lop s is me galop hylla of protein expression by using two-dimensional gel electrophoresis (2-DE). Methods: isn this study, proteinsfrom the tender leaves of A mpelopsis megalophylla were precipitated by Tris-phenol extraction and ammonium acetate methanol precipitation. Then using 2-DE technology to isolate protein.Finally, 2-DE was analyzed by the scanner, and got protein characteristic maps. Result:According to the differences in ratio of gray value, the seven differential protein points were chosen to identify the mass spectrum. At present, two proteins were identified, namely the hypothetical protein and the unnamed protein product. Conclusion: the application of 2-DE technology for A mpelopsis megalophyllathe active ingredient of protein expression at the level of molecular research laid a good foundation.