Objective:To summarize the experience of complete repair of tetralogy of Fallot in infants and young children.Method:A retrospective review of one-stage repair of tetralogy of Fallot(TOF)performed in 87 consecutive patients no more than 3 years old was made.Follow-ups were obtained from clinic appointments.The patients were divided into three groups according to age at surgery:group A(30mmHg)in 3 cases,residual VSD in 3 cases,low cardiac output syndrome in 6 patients.There were no late death and reoperation during follow-up period of 1 to 78 months (36?27 months).74(90.2%)patients were in NewYork Heart Association(NYHA)functional class I,and the other 9(10.8%)were in class II.Group A had significantly longer ventilation,ICU stay and inotropic support time than group B and group C.A slight increase in the incidence of PR,RVOTO in group C.Conclusion:Early one-stage repair of TOF in patients in infants and young children has a low operative mortality and can provide excellent asymptomatic mid-term survival,and the optimal age for elective repair of TOF is 3 to 12 months of age.

Objective:To explore relation of Cerebral electric impedance coefficient,S100,neuron specific enolase(NSE)in serum and brain damage by the cardopulmonary bypass(CPB)in infants.Methods:40 children in 1～2-year-old who were diagnosed to be non-cyanotic congenital heart disease were selected chosen as experimental A and B group.The BOEN-BE No-injury Brain-edema-monitor was used to test Cerebral electric impedance coefficient.The selum concentrations of S100 and NSE in the different time of cardiopul-monary bypass(CPB)were measured with enzyme linked immunosorbent assa.Results:Cerebral electric impedance coefficient,S100 and NSE were significantly increased from the beginning to the end in CPB and significantly decreased gradually after operation in the dif-ferent time in A and B group(P

Objective To investigate the activation of matrix metalloproteinase (MMP)9 by acute arterial injury and the involved signaling mechanism in murine femoral artery. Methods In the C57BL/6 mice femoral artery denudation injury were performed. Total protein and membrane protein extracts were prepared from targeted arteries. The MMP 9 activity was measured by zymography assay, the expressions of MMP 9 antigen and protein kinase C (PKC) isoforms were measured by Western blot. Seventy-two hours after mice fed with PKCβ inhibitor (ruboxistaurin), the denudation injury triggered MMP 9 activation was reassessed. Results Within 4-24 hours after denudation injury, MMP 9 activity in femoral arteries was significantly increased, with a peak induction of (99.3±9.5) times the sham control (F=51.49,P＜0.01) at 8 h. MMP 9 antigen increased in parallel with MMP 9 activity. Within 15-120 minutes after denudation injury, there was a significant induction of PKCβⅡ in membrane fraction of femoral arteries, with a maximum induction of (7.50±0.60) times the sham control (F=207.06,P＜0.01)at 30 min. Injury-induced MMP 9 activation was significantly inhibited by ruboxistaurin. Conclusions MMP 9 activation is, at least in part, mediated by PKCβ in acute arterial denudation injury, it highlights the new target for therapeutic intervention to suppress the over-activation of MMP 9, which plays a critical role in restenosis.

Air Pollution ; Health Status ; Humans

Objective:To study the effects of somatostatin on portal pressure and operative complications in patients of portal hypertension treated with porta-azygous disconnection. Methods:Twenty patients with portal hypertension were treated with azygoportal disconnection. The severity of liver disease according to the Child-Pugh's grade was B in all patients. The patients were divided into two groups. The patients in group A were treated with intravenous infusion of somatostatin (6 mg/24 h) one day before operation and five days after operation. The patients in group B were treated with azygoportal disconnection without the use of somatostatin. The portal pressure, the volume of bleeding during operation, and the ascites and complications after operation were evaluated. The serum concentration of GLU, NO and ET-1 were determined. Results:The portal pressure and GLU concentration in patients of group A were lower than those in patients of group B. There were no differences of NO, ET-1 in patients between group A and group B. The volume of bleeding, ascites and complications in patients of group B were higher than in patients of group A. Conclusion:The use of somatostatin before and after porta-azygous disconnection can decrease portal pressure. It is beneficial in reducing the volume of bleeding in operation, ascites and complications after operation.

DNA Damage ; drug effects ; Formaldehyde ; toxicity ; Mutagenicity Tests

Brain Death ; diagnosis ; Breath Tests ; Cerebrovascular Circulation ; Child ; Child, Preschool ; Coma ; diagnosis ; Electroencephalography ; Humans ; Infant ; Infant, Newborn ; Neurologic Examination ; Practice Guidelines as Topic

Animals ; Curcumin ; pharmacology ; therapeutic use ; Humans ; Liver Cirrhosis ; drug therapy

Antihypertensive Agents ; therapeutic use ; Humans ; Hypertension ; therapy ; Practice Guidelines as Topic

Adult ; Anemia, Hemolytic, Autoimmune ; etiology ; therapy ; Antibodies, Monoclonal, Murine-Derived ; administration & dosage ; therapeutic use ; Cord Blood Stem Cell Transplantation ; adverse effects ; Female ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; surgery ; Rituximab