Air Pollution ; Health Status ; Humans

Mitochondrial DNA(mtDNA),a double-stranded circular molecular,encodes several genes essential for mitochondrial and cellular functions despite its small amount of genetic information.Damage of mtDNA can lead to changes of cellular structures and functions and this fact is drawing more and more attention.This review summarized the recent research about mtDNA damage and its influence on cells.

Objective To understand the mastery situation of the knowledge about common infectious diseases among the prima-ry and middle school students,in order to provide the evidence for the prevention and treatment of infectious diseases.Methods The stratified random cluster sampling method was adopted.The survey was performed by the questionnaire form.The data were analyzed by the Chi square test,logistic regression model in the SPSS17.00 statistical software.Results Among the primary and middle school students in Chongqing,the students mastering 75% of the basic knowledge about infectious diseases accounted for 24.6% of the total respondents (P<0.01).From the view of the distribution of disease entities,the top six diseases with the awareness rate of less than 60% were hepatitis B,bacterial dysentery,cholera,measles,pinworm and rabies respectively(P<0.01), whose awareness rates are under 60%(P<0.01).As for the level of mastery among different grades,the senior middle school students had a better knowledge of tuberculosis,flu,SARS,AIDS,hepatitis B,poliomyelitis,etc(P<0.01).Comparatively,the pri-mary school students grasped the knowledge of hand-foot-and-mouth disease and pinworm better(P<0.01).Conclusion The pri-mary and middle school students have a low awareness rate of infectious diseases.Pertinently strengthening the popularization of the knowledge about infectious diseases is the powerful measure for reducing the occurrence of infectious diseases and promote the healthy development of students.

Mitochondrial DNA(mtDNA), a double-stranded circular molecular, encodes several genes essential for mitochondrial and cellular functions despite its small amount of genetic information. Damage of mtDNA can lead to changes of cellular structures and functions and this fact is drawing more and more attention. This review summarized the recent research about mtDNA damage and its influence on cells.

DNA Damage ; drug effects ; Formaldehyde ; toxicity ; Mutagenicity Tests

In the study, the inhibitory effect of epigallocatechin gallate (EGCG) on Calcium oxalate nephrolithiasis and its possible mechanism were investigated. The rat Calcium oxalate nephrolithiasis model was induced through the combined oral administration of ethylene glycol and ammonium chloride, which was intervened with EGCG. Rat blood samples were collected to detect blood creatinine (Cr), blood urea nitrogen (BUN) and blood calcium. Rat urine samples were collected to observe and compare 24-hour urine volume, oxalic acid (Ox) and calcium in urine. Renal samples were collected to prepare tissue slices and observe the pathological changes in Calcium oxalate nephrolithiasis. The expression of osteopontin (OPN) in renal tissues was evaluated by Real-time PCR and Western blot. According to the results, compared with normal rats, rats in the nephrolithiasis model showed significant increases in Cr, BUN, urine Calcium, urine Ox and renal OPN expression (P < 0.05), but obvious decrease in 24-hour urine volume (P < 0.05); Compared with rats with nephrolithiasis, those processed with EGCG revealed remarkable declines in Cr, BUN, urine Calcium and urine Ox (P < 0.05), with significant rise in 24-hour urine volume (P < 0.05) in a concentration-dependent manner. Additionally, compared with the control group, nephrolithiasis rats showed significant pathological changes in Calcium oxalate calculus. After ECCG treatment, the renal pathological changes and OPN expression attenuated significantly in a concentration-dependent manner. The results showed that EGCG inhibits the formation of calcium oxalate nephrolithiasis in rats and shows a notable protective effect on renal functions.
Animals ; Blood Urea Nitrogen ; Calcium ; blood ; Calcium Oxalate ; metabolism ; Catechin ; administration & dosage ; analogs & derivatives ; Creatinine ; blood ; Disease Models, Animal ; Humans ; Kidney ; drug effects ; metabolism ; Male ; Nephrolithiasis ; blood ; drug therapy ; genetics ; Osteopontin ; genetics ; metabolism ; Rats ; Rats, Wistar

Objective To study the subchronic toxicity of sodium fluoride on mRNA expression of estrogen receptor(ER) in female mice.Methods Forty female mice were randomly divided into 4 groups according to body mass,10 in each group,and exposed to sodium fluoride solution(0,1,5,25 mg/L) through drinking water in control,low-dose,medium-dose and high-dose groups for 12 weeks.The expression levels of ERα,ER3 mRNA in ovarian tissues were determined by RT-PCR method.Results The relative expression levels of ERα,ERβ mRNA in control,low-,medium-and high-dose groups were 0.7028 + 0.0474,0.7195 ± 0.0552,0.6479 ± 0.0590,0.5684 ± 0.0513 and 0.8418 ± 0.0131,0.7729 ± 0.0974,0.7610 ± 0.0984,0.8026 ± 0.0234,respectively.The difference between high-dose and control groups of the expression level of ERα was statistically significant (P ＜ 0.05).Conclusions The subchronic toxicity of fluoride can decrease the expression of estrogen receptor in ovarian tissue,which may have a certain effect on reproductive development of female mice.

Humans ; Occupational Health ; Polymorphism, Single Nucleotide

Objective To investigate the effect of phosphatidylinositol 3-kinase(PI3K) inhibitor wortmannin on migration and invasion of human thyroid follicular carcinoma cell line CGTHW-1 and its mechanism.Methods MTT colorimetric assay was used to select the optimal concentration and time of giving wortmannin.CGTHW-1 cells used in present study were divided into control group and experimental group(cultured in the medium with or without blood serum).Cells of experimental group were treated with wortmannin,and that of control group were given no treatment.The migration and invasion ability of CGTHW-1 cells was detected by scratch test and Transwell chamber assay respectively.Microfilament fluorescence staining with fluorescein isothiocyanate(FITC)-labeled phalloidin was performed to observe the morphologic changes in CGTHW-1 cells.The expressions of Rac1 protein and mRNA in CGTHW-1 cells were examined by immunocytochemistry staining and semiquantitative RT-PCR.Results When CGTHW-1 cells were cultured in serum medium,the migrating speed of the cells slowed down,and the number of cells passing through the Transwell chamber reduced significantly after being treated with wortmannin in experimental group(13.8?3.03) than in control group(41.6?6.95,P0.05).Conclusions Wortmannin can effectively depress the migration and invasion of human thyroid follicular carcinoma cells,and the depression effect may be due to cytoskeleton rearrangement induced by inhibiting the expression of Rac1(the downstream molecule of PI3K).PI3K and Rac1 are probably potential targets for human thyroid cancer treatment,which may provide a theoretical evidence for the drug treatment of thyroid cancer.

Heat Stress Disorders ; etiology ; Humans ; Mining ; Occupational Diseases