Objective: To investigate the application of microemulsion TLC in separation and identification of various effective compositions in traditional Chinese patent medicines. Methods: The various compositions in Kangganmao Granules and Fangfeng Tongsheng Pills were separated and identified simultaneously on polyamide film with six kinds of SDS/n C 4H 9OH/n C 7H 16 /H 2O microemulsions as the mobile phases. Affections of kinds and acidity on separationg results of microemulsions were investigated. Results: The baicalin, chlorogenic acid, Saposhnikovia and glycyrrhiza in Kangganmao Gronules and the emodin, chrysophanol, baicalin, Saposhnikovia and Glycyrrhizae in Fangfeng Tongsheng Pills may be separated and identified simultaneously with microemulsion (containing 75% water) formic acid (9∶1) as mobile phase. Conclusion: Microemulsion TLC is an efficient, simple new method for the separation and identification of various compositions in traditional Chinese patent medicines.

A total of 40 adult black-mice was used and divided into two groups for expeiment. Group A was irradiated by UVB only and Group B had SPF 15 sunscreen a.pplied to the back followed by irradiation by UVB. Each group was divided again into 5 subgroups according to the days of UVB irradiation frcm 2 to 10 days. A Waldmann combination UVA+UVB Radiation Treatment Cabin 8001 was used as the light source and the UVB dosage was 50 mJ/cm2 daily. Skin specimens were taken 24 hours after the last irradiation. Histologic changes in epidermis were reviewed by the light microscope. In group A, the characteristic sunburn cells(SBC) were observed with 100 mJ/ cm2. SBC number was maximum with 400 mJ/cm2. The other epidermal changes were parakerat.osis, crusts, atypical cells, and mitoses of basal cells, which showed graded responses to the UVB doses. Pretreatment with the sunscreen completely prevented these changes.
Adult ; Epidermis ; Humans ; Keratinocytes* ; Mitosis ; Skin ; Sunburn

No abstract available.
Embryonic Development* ; Embryonic Structures* ; Female ; Ovulation* ; Pregnancy

OBJECTIVES: Cell adhesion molecules may play a role in integrating amnionic membrane. The objective of this study was to prove E-cadherin mRNA and proteins in cultured human amnionic cells. METHODS: We cultured amnionic cells from 4 women undergoing cesarean section without labor. E-cadherin was determined by immunohistochemistry and Western ligend blotting. To demonstrate E-cadherin mRNA, reverse transcription-polymerase chain reaction was performed. RESULTS: On immunohistochemistry, E-cadherin was abundantly showed on the cytoplasm of the cells. Western ligend blotting showed clear 120 kDa bands on four specimens, and relatively weak band on one specimen. Reverse transcription-polymerase chain reaction showed 432 BP bands. CONCLUSION: We proved E-cadherin and its mRNA by immunohistochemistry, Western ligend blotting and reverse transcription-polymerase chain reaction in cultured human amnionic cells.
Amnion* ; Cadherins* ; Cell Adhesion Molecules ; Cesarean Section ; Cytoplasm ; Female ; Humans ; Immunohistochemistry ; Membranes ; Pregnancy ; RNA, Messenger

PURPOSE: To evaluate high signal intensity of nontumorous conditions of corpus callosum on T2-weighted MR images. MATERIALS AND METHODS: Fourty nine patients with nontumorous high signal intensities involving corpus callosum on sagittal T2-weighted image were restrospectively analyzed. Nontumorous condition of corpus callosum were diffuse axonal injury(DAI, 19 cases), cerebral infarctions(16 cases), multiple sclerosis(MS, 5 cases), Wilson's disease(2 cases) and hydrocephalus(7 cases) that were diagnosed by clinical and MR findings. Numbers, configuration, involved thickness and sites of high signal intensities of corpus callosum were analyzed. RESULTS: DAI and infarctions showed either single or multiple lesions. MS and hydrocephalus showed multiple lesions, but Wilson's diseases showed single lesion. In DAI, infarctions and MS the lesions involved any part of corpus callosum, splenium in WIIson's disease, and all parts of corpus callosum in hydrocephalus. Wilson's disease showed only partial thickness involvement, and others involved partial or full thickness of corpus callosum. Configuration of high signal intensity was linear in most cases of hydrocephalus, and oval in Wilson's disease, and oval and confluent in MS, and variable in DAI and infarctions. CONCLUSION: High signal intensities of nontumorous conditions of corpus callosum revealed variable findings, and therefore, analysis of nontumorous high signal intensities of corpus callosum is not made by only MR findings but by conjuction with clinical aspects.
Axons ; Corpus Callosum* ; Hepatolenticular Degeneration ; Humans ; Hydrocephalus ; Infarction

We report a case of a 41-year-old female with acrogeria. She was in good health except for the prominent atrophy over the hands and feet. On microscopic examination of a biopsy specimen from the atropl6c skin, elastic fibers were clumped and fragmented. Electronmicroscopy revealed amorphous and granulous pseudoelastin, which is presumed to be consistent with acrogeria. Our patient had acro-osteolysis and an extra chromosome in addition to the normal 23 pairs of chromosomes in one of 20 cells examined, which have been very rarely reported in the literature. Further studies are needed to find out whether this chromosomal aberration might contribute to the pathogenesis.
Acro-Osteolysis ; Adult ; Atrophy ; Biopsy ; Chromosome Aberrations ; Elastic Tissue ; Female ; Foot ; Hand ; Humans ; Skin

Authors carried out the experimental study to investigate whether the Paragonimus westermani would uptake the blood in the parasitic definitive host, and also tried for the amount and distribution of the blood which would be harbored in a worm body, and so on. Experimental animals used in this study were dogs and cats, and they were infected with metacercariae of P. westermani. Several months later of the infection, Cr(51) tagged red blood cells were administered to each animal, and the animals were sacrificed and autopsy was done after 24 or 48 hrs of the administration. One part of the detected P. westermani adult worms of each animal were bred in Tyrode's solution and the others were killed by fixing in formalin solution. By Well-type scintillation counter, the radioactivities were measured for the alive and killed worms and of the blood of each experimental animal which was collected at autopsy . In conclusion, it was found that P. westermani worms uptake blood in parasitic definitive host, and amount of the blood harboring in a worm of 56 infection-days(size of worm; 4 x 3 x 1 mm) was 0.002 ml, 0.004 or 0.005 ml in 103 infection-days(size of worm: 8 x 5 x 3 mm) and 0.011 ml in 217 infection-days(size of worm: 9 x 7 x 4 mm). Almost of the whole blood uptaked in the worms was distributed in intestine. It was observed that the blood uptaked in the worm body was excreted continuously through the excretory pore, so that the radioactivity could not measured almost completely in cases of 24 hrs-breeding worms.
parasitology ; helminth ; trematoda ; Paragonimus westermani ; metabolism ; biochemistry ; red blood cell ; Cr(51) ; Tyrode's solution

The peripheral blood eosinophil and serum IgE level in 53 patients with atopic dermatitis (AD) were measured. The patients were divided into 2 groups by severity(mild and severe grous) and into 3 groups by the associated respiratory atopic deseases and/or their family history : respiratory group(patient, with both AD and respiratory atopy), family history group (patient with both AD and family history of respiratory atopy), and atopic group(patient with neither respiratory atopy nor family history of respiratorv atopy). We designed to study which factors are important in the elevation of serum IgE and peripheral blood eosinophil level in AD, and to know possible relationships between the serum eosinophil and IgE level and the several groups of AD. The results are as follows : 1. Peripheral eosinophil counts were higher in severe group(224.8/mm) than in mild group (180.0/mm)(p<0.05). 2. Peripheral eosinophil counts were 220.0/mm in atopic group, l65.0/mm in family history group, and 332.4/mm in respiratory group, but there was no stitistically significant difference among 3 groups. This suggests that concomitant respiatitiry atopy or a family history of respiratory atopy is not an important factor in the elvation of peripheral blood eosinophil counts in AD. 3. Serum IgE was higher in severe group(443.2IU/ml) than in mild group(231.5IU/ml)(p<0.05). 4. Serum IgE level in respiratory group(754.6IU/ml) were signifiiantlly higher than in atopic (286.6IU/ml) or family history group(342.0IU/ml)(p<0,01). But there was no significant. difference between family and atopic group. This result suggests that concomittent respiratory atopy is a potential factor in elevation of serurn Igi in AD. 5. Slightly high correlation between peripheral blood and IgE level appeared in all 53 patients (r=0.434) and severe group(r=0.480). But, respiratory group(r=0.060), family history group(r=0.111) and atopic group(r=0.202) showed poor relationships.
Dermatitis, Atopic* ; Eosinophils* ; Humans ; Immunoglobulin E*

Various epidermal changes overlying a dermatofibroma can be induced through the proliferation of pluripotential epidermal cells by the stimulation of mesenchymal factors. The authors report a case of dermatofibroma in a 55-year-old male who had a reddish brown, asymptomatic nodule of 5 years duration on his left forearm. The biopsy specimen revealed a dermatofibroma of cellular type associated with epidermal changes: (1) acanthosis; (2) elongation of rete ridges; (3) thin interlacing epidermal strands with increased pigmentation resembling seborrheic keratosis or fibroepithelioma; (4) immature hair follicles and sebaceous lobules; (5) hair germ like buddings; (6) superficial basal cell epithelioma-like proliferations.
Biopsy ; Forearm ; Hair ; Hair Follicle ; Histiocytoma, Benign Fibrous* ; Humans ; Keratosis, Seborrheic ; Male ; Middle Aged ; Pigmentation