BACKGROUND: Recently, it has been established that plateletpheresis needs more efficiency and shorter processing time. Fenwall laboratories developed a new collection chamber for CS-3000 Plus, PLT-30TM collection chamber, which can reduce the processing time with efficient collection. We evaluated the PLT-30TM collection chamber by comparing it with A-35 collection chamber that has been used as a standard collection chamber of CS-3000 Plus us. METHODS: Thirty platelet collection procedures were performed using the CS 3000 Plus with A-35 collection chamber and PLT-30TM collection chamber. The changes of the hematologic parameters between pre- and post-donation in donors and the total platelets yields and the contaminated WBCs in the plateletpheresis products were evaluated. In processing, the yield predictor calibration was adjusted to 1.00 and 1.13 in A-35 and PLT-30TM respectively. Yield predictors of pheresis were the same as 3.5x1011 in both and end point volumes were calculated from the CS-3000 Plus. Processing volume and processing times were compared between A-35 and PLT-30TM groups. RESULTS: With PLT-30TM collection chamber, 3.38+/-0.72x1011/L platelets were harvested, whereas 3.20+/- 0.73x1011/L were collected with A-35 collection chamber, which was not significantly different. But processing time with the PLT-30TM collection chamber was more reduced than that with the A-35 collection chamber by about 20 minutes (PLT-30TM : 88.6+/-8.4 min, A-35 : 106.7+/-11.7min). Collection efficiency of PLT-30TM chamber was 50.7+/-12.5% and that of A-35 chamber was 44.4 + 8.8%. The leukocyte contamination of the platelet concentrates were not statistically different(PLT-30TM: 0.0-3.6x106, A-35 : 0.1-4.1x106). CONCLUSIONS: PLT-30TM collection chamber has the advantages of shortening the donation time and decreasing the processing volume with better collection efficiency and flexibility of platelet concentrate volume.
Blood Component Removal ; Blood Platelets ; Calibration ; Humans ; Leukocytes ; Plateletpheresis ; Pliability ; Tissue Donors

The aim of this study is to develop an animal model of polyps that is more suitable for further polyp studies by semiquantitatively analyzing the effects of Pseudomonas aeruginosa infection and/or mechanical trauma to the sinus mucosa, based on the epithelial rupture theory, on polyp formation in the rabbit maxillary sinuses. The natural ostia were occluded in 30 Pasturellafree New Zealand white rabbits. All rabbits were divided into three groups according to the following modes of manipulation on the right maxillary sinus: inoculation with P. aeruginosa (group A), brushing of the sinus mucosa (group B), and inoculation with P. aeruginosa accompanied by brushing of the mucosa (group C). The left maxillary sinuses were used as the control. After four weeks, macroscopic polyps and light microscopic polyps and/or polypoid mucosa were semiquantitatively analyzed. The number of macroscopic polyps per sinus was significantly larger in groups A (1.0 polyps) and C (1.3 polyps) than in group B (0.0 polyps). Microscopic examination revealed significantly higher levels of polyps and/or polypoid mucosa in groups A and C than in group B. The results of this study suggest that a rabbit model of polyps using P. aeruginosa is suitable for further polyp studies and that induction of this type of model needs no mechanical trauma to the sinus mucosa.
Maxillary Sinus* ; Models, Animal ; Mucous Membrane* ; Polyps* ; Pseudomonas aeruginosa* ; Pseudomonas* ; Rabbits ; Rupture

PURPOSE: To investigate the causes of structural degradation in Ti-6A1-4V alloy. MATERIALS AND METHODS: We prepared 20 metal specimens by cutting the fringe of 4 screw holes of tibial components taken from 5 cases of MG TKS(unused: 1, 3 years used: 2, 6 years used: 2). The structure and composition of specimens were analyzed by Scanning electron microscope(SEM), Energy dispersive spectrometer(EDS), and hardness tester. RESULTS: In soft tissue, titanium contained giant cells were observed by H-E stain, and Ti and Va peaks were detected by EDS. SEM showed that the unused specimens had normal metal fiber mesh texture, and the texture began to disappear from the surface of specimens after 3 years later. There were no metal fiber mesh, but black deposits were detected in specimens of 6 years. EDS revealed that Ti, Va, and Al peaks were appeared in the unused specimens, whereas 0 and C peaks in specimens of 3 years a#od 0, C, P, S, Ca and Cl peaks in specimens of 6 years were obtained. The hardness was decreased with the used period. CONCLUSION: It seemed that various molecular components of human body including carbon, hydrogen and oxygen influenced the metal elements of alloy to degrade in their structure.
Alloys* ; Carbon ; Giant Cells ; Hardness ; Hardness Tests ; Human Body ; Humans* ; Hydrogen ; Oxygen ; Titanium

BACKGROUND AND OBJECTIVES: Pathogenesis, definition, and treatment of sinonasal polyps remain uncertain. To elucidate them, many studies on polyps have been made. However, recently reported studies on polyps using animal models seldom provided comparative data on polyp formation in various conditions. The aim of this study is to develop an animal model of polyps which is more suitable for further polyp studies. We attempted a semiquantitative analysis on the effects of mechnical trauma to the sinus mucosa on formation of polyps and polypoid mucosa in duction of rabbit maxillary sinusitis by Streptococcus pneumoniae and compared the prevalences of polyps under the different conditions. MATERIALS AND METHODS: The natural ostia of both sinuses were occluded in 10 Pasturella-free New Zealand white rabbits. Both sinuses were inoculated with S. pneumoniae, without brushing the mucosa in the left maxillary sinuses (control group) and with brushing in the right sinuses (experimental group). After four weeks, all sinuses were re-opened. Macroscopic polyp and light microscopic polyps and/or polypoid mucosa were semiquantitatively analyzed and each prevalence was compared between the two groups. RESULTS: The number of macroscopic polyps per sinus was significantly larger in the experimental group (1.1 polyps) than that in the control group (0.1 polyps). Microscopic examination revealed significantly more polyps and/or polypoid mucosa in the experimental group than in the control group. CONCLUSIONS: Our results suggest that mechanically traumatizing the sinus mucosa, such as brushing, is more helpful to polyp formation when a rabbit model of polyps is to be induced by S. pneumoniae.
Maxillary Sinus* ; Maxillary Sinusitis* ; Models, Animal ; Mucous Membrane* ; Pneumonia ; Polyps* ; Prevalence ; Rabbits* ; Streptococcus pneumoniae* ; Streptococcus*