BACKGROUND: Arterial blood gas analysis are highly susceptible to preanalytic error due to improper method of obtaining or handling the blood sample before analysis. The error in measurement of blood gas analysis are loss of CO2 by exposure to atmospheric air, effect of anticoagulant itself, temperature difference between the experimental subject and the measuring electrode and metabolic change which occur between blood sampling and measurement. METHOD: To study the effect of the delay in estimation of blood gas and drawn blood on values of blood gas partial pressure and pH. Blood sample were divided into 2 groups according to the method of storage, group I stored at 24~25degrees C(room temperature) under anaerobic condition. ;group II stored at 0~4degrees C(refrigerator) under anaerobic condition. The samples were analyzed by time interval through 180 minutes in each group. RESULTS: The result were as follows: 1) PaO2 decreased significantly after 10 mins in group I, whereas it decreased significantly after 20 mins in group II. 2) PaO2 increased significantly after 20 mins in group I, whereas it increased significantly after 120 mins in group II. 3) pH decreased significantly after 60 mins in group I, whereas it decreased significantly after 120 mins in group II. 4) No significant changes of bicarbonate and SaO2 were noted in each group CONCLUSION: From above results, it would be advisable to analyze the sample in a short period of time or to store in a refrigerator when the measuring will be delayed. So we highly recommend that blood gas analysis should be performed as soon as possible after sampling, especially within 10 minutes.
Blood Gas Analysis ; Electrodes ; Hydrogen-Ion Concentration ; Partial Pressure

No abstract available.
Humans ; Muscle Spasticity*

No abstract available.
Adenoma, Pleomorphic*

We present two cases of lymphangioma circumscriptum according to the clinical pattern and histopathological findings, one with lesions on the tongue and the other with lesions of the oral mucous membrane. The first case was a 5 year-old boy with a plaque of grouped transluscent vesicles resembling frog's spawn on ventral surface of the tongue of three years duration. The second case, also a 5 year-old boy, had a lymphangioma circumscriptum lesion in the right buccal mucous membrane which developed several months after birth and eventually produeed swelling of the right cheek. The histopathological findings of both cases showed variable-sized dilated lymphatic channels lined by one layer of normal endothelial cells, extending to the deep dermis, subcutaneous tissue and muscle layer and resembling the spongy type variant of classical lymphangoima circumscriptum using the classification of lymphangioma of Peacheyet al.
Cheek ; Child, Preschool ; Classification ; Dermis ; Endothelial Cells ; Humans ; Lymphangioma ; Male ; Mouth* ; Mucous Membrane ; Parturition ; Subcutaneous Tissue ; Tongue

Cutis naarmorata telangiectatica congenita(CMTC) was first lesribed by Van Lohuizen in 1992. Cases of CMTC were reported later under various names, includir gan Lohuizens syndrome, congenital generalized phlebectasia. congential phlebectasia and reticulatever scular nevus. It is charaterized by persistent livedo reticularis, telangiectasia and phlectnsia from birch hand atrophy and superficial ulceration of the overlying skin are uncornmonly encountered. They have a steady improvement with time, however various congenital abnormalties, such as varicosities, hen ariomas, hypoplasia or hypertrophy of soft tissue and bones, rarely generalized congenital fibrornatos ectal and genital anomaliies may be associated in approxirnately 50 percent of patients. In case 1, the patient was 45-day-old female infant with reticulated mottling and atrophy of the skin on the right leg. In case 2, the patient was 30-day-old male infant who depressed, bluish, and reticulated patches on the both upprarms, right side trunk, buttock and loen leg. Decreased circumferences of the affected extremities vas observed in case 2. No other assotated anomalies were found in physical and X-ray examinatiorwlin both cases. The skin biiopsy of bothed similar findings, which revealed mild dilatation and proliferation of capillaries, mild inflammatoryel infiltration and extravasated red blood cells in the dermis. The clinical follow-up, alneast all of th lisions disappeared spont.anously in 1 year at case 1, and some of lesions also disappeared in 3 month, 11 case 2. In addition, we reviewed and summerized the reported cases in Korea.
Atrophy ; Betula ; Buttocks ; Capillaries ; Dermis ; Dilatation ; Erythrocytes ; Extremities ; Female ; Follow-Up Studies ; Hand ; Humans ; Hypertrophy ; Infant ; Korea ; Leg ; Livedo Reticularis ; Male ; Nevus ; Skin ; Telangiectasis ; Ulcer

Eccrine carcinoma does not have a characteristic clinical appreranc, but does have a high incidence of metastatic spread. It may arise anywhere in the skin. The microscopic patterns that of an adenocarcinoma, which may be confused with metastatic adenocarcinoma. In the classical type the histologic features varies from fairy well-differentiated tubular structures in some areaslo anaplastic carcinoma in other areas. The patient was a 51-year-old man who showed a painful, tender, right red-calored, 4 x 5crn-sized tumor mass on the right angle of the lower lip for ten years. In the diological examinations multiple metastatic foci were seen on both lung fields, Histopathologic findings of the skin and lung biopsy specimens revealed that of the classic type of eccrine carcinoma like the bove descriptions. Immunocytochemical studies showed a positive reactivity to anti-cytokeratin anti-ibody, anti-CEA antibody, and anti-S-100 protein on the skin and lung biopsy specimens and no he reactivity to anti-S-100 protein on the lung mass. Electronrnicroscopic findings revealed eccrine glaidlar structures such as nunierous mitochondria and dense core granules in the cytoplasm of neoplastic cells. We report a case of eccririe carcinoma with lung metastases baed on the clinical, radiological and histologic findings.
Adenocarcinoma ; Biopsy ; Carcinoma ; Cytoplasm ; Humans ; Incidence ; Lip ; Lung* ; Middle Aged ; Mitochondria ; Neoplasm Metastasis* ; Skin

An etiologic agent in seborrheic dermatitis is now considered to be due to Pityrosporum ovale. The connection between the yeast and the disease has been clearly dernonstrated in a number of patients treatd with antifungal agents. However, the fact, that Pityrosporum ovale (Pityrosporum orbiculare) belongs to the normal human cutaneou. flora makes it difficult, to explain the role of the organism in seborrheic dermatitis. In this clinical study, twenty eight subjects were divided into two group by treatment regimen of topical 2% ketoconazole shampoo(TKS)and 0.3% prednisolone valerate solution (PVS). Before and during the four weeks treatment periods, the number of Pityrosporum ovale on the scalp were evaluated by scrub Giemsa staining method, which estimat,ed by score scale, under direct. microscopic examination. Also clinical symptoms including erythema, scales and itching were recorded by scores every week and compared between two treatment group. Topical application of 2% ketoconazole shampoo(TKS) is a very effective treatment regimen to reduce the yeasr cell score(from 5.8+1.3 to 1.8+1.4) than PVS(from 6.4+1.3 to 3.5+1.5), significantly(p<0.05). So the results of this study acconsistent with the view that density of Pityrosgourum ovale plays a role in the cause and course of seborcheic derrnatitis on the scalp. And antifungal agent(TKS) shows favorable effects ori tnis disease clinically and mycologically.
Antifungal Agents ; Azure Stains ; Dermatitis, Seborrheic* ; Erythema ; Humans ; Ketoconazole* ; Malassezia ; Prednisolone ; Pruritus ; Scalp* ; Weights and Measures ; Yeasts

BACKGROUND: Diabetes mellitus is a heterogeneous group of disorders characterized by high serum glucose levels and by disturbances of carbohydrate and lipid metabolism. There are many cutaneous signs of this common endocrinopathy, such as nercobiosis lipoidica diabeticorum, diabetic bullosis, shin spot, diabetic pruritus, etc. OBJECTIVE: In this study, we investigated whether extracellular matrix gene expression in cultured skin fibroblast is influenced by insulin and Insulin-like growth factor-I(IGF-I). METHOD: Total RNA was isolated from insulin or IGF-I treated human skin fibroblasts. The Northern blot and slot-blot hybridization were then conducted. RESULTS: The mRNA levels of pro α1(I) collagen, pro α1(I11) collagen, fibronectin in insulin and IGF-I treated normal skin fibroblasts increased compared with untreated normal skin fibroblasts. CONCLUSION: Our results show that insulin and IGF-I stimulate collagen formation in normal skin fibroblast at physiological concentrations. Therefore, these demonstrate that insulin can modulate the expression of extracellular matrix gene.
Blood Glucose ; Blotting, Northern ; Collagen ; Diabetes Mellitus ; Extracellular Matrix* ; Fibroblasts* ; Fibronectins ; Gene Expression ; Humans ; Insulin* ; Insulin-Like Growth Factor I ; Lipid Metabolism ; Methods ; Pruritus ; RNA ; RNA, Messenger ; Skin*

Mycetoma is a clinical syndrome of localized, indolent, deforming, swollen lesions and sinuses, involving cutaneous and subcutaneous tissues, fascia, and bone. Its lesions. are composed of suppurat.ing abscesses, granulomata, and dra ning sinuses and the etiologic agents are wide variety of bacteria (actinomycotic mycetoma) and fungi(eumcotic mycetoma) from plant and soil. The patient was a 65-year-old female and a farnier. She was referred by the skin lesion loalized on the dorsum of her lefther left hand and wrist for three months. It showed multiple subcutaneclis nodules, abscesses, and discharging fitula based on the erythematous plaque. The skin biopsy frorn the lesion revealed the PAS positive hyphae in the granulocytic infiltration. Fungus cult,ure with a piece of tissue on sabouraud media shows hin, floccose, white colonies. Slide culture and biochemical idcrtification of pathogen led to the final diagnosis as Mycetoma caused by Pseudallescheria boydii(Monosporium apiospermum). The lesions disappeared gradualy following systemic oral administratior of Itraconazole 200mg daily for six weeks.
Abscess ; Aged ; Bacteria ; Biopsy ; Diagnosis ; Fascia ; Female ; Fungi ; Hand ; Humans ; Hyphae ; Itraconazole ; Mycetoma* ; Plants ; Pseudallescheria* ; Skin ; Soil ; Subcutaneous Tissue ; Wrist

There are several ELISA(enzyme linked immunosobent assay) kits to be highly sensitive and specific in the screening test for antibodies to HIV. However, these have several problems such as high false-positive rate, low reproducibility and complex test procedures. A new serological test, the gelatin particle agglutination test(PAT), was developed and reported as good test having the same sensitivity with ELISA, and simple and easy procedure. The purpose of this study is com parison of two screening tests, ELISA and PAT, for detection of antibodies to HIV In one hundred two prostitutes, who used to contact with foreigners such as U.S. army personels, 10 syphilitic patients in Chonnam University hospital and 3 HIV infected patients, the serological results were equal in both tests, so there. was 100% concordance rate of seroreactivity between ELISA and PAT. Among 13 serum samples with false positive in ELISA test, only one sample revealed also reactive in PAT, which suggest indirectly us for PAT to be more specific than ELISA. The quantitative test with PAT of 4 consecutive serums during 4 months in the AIDS patient with Kaposi's sarcoma. However, in overall serum titer, the AIDS patient was higher than asymptomatic infected person. Thus the PAT test should be also useful for the mass screcning and quantitative test of anti-HIV antibody, and is highly recommedable for detection of HIV infection because the test can be performed with simple procedure and techique in addition ecoomically low cost and shortened time for the test.
Agglutination ; Antibodies ; Emigrants and Immigrants ; Enzyme-Linked Immunosorbent Assay* ; Gelatin ; HIV ; HIV Infections ; Humans ; Jeollanam-do ; Mass Screening* ; Sarcoma, Kaposi ; Serologic Tests ; Sex Workers