Objective To study the effect of Dragon’s blood on the expression of GSK-3? and the volume of hepatic glycogen and muscle glycogen in the liver and muscle of mice with DM. Method Selecting 120 mice of Kunming strain,and randomly dividing them into six groups:normal group,DM group,Dragon’s blood groups with three dosage 125,250,500 mg/(kg?d) and C4H11N5?HCl group. Dragon’s blood groups were given Dragon’s blood by introgastric (ig) everyday for 7 d. C4H11N5?HCl group were given C4H11N5?HCl 1 mg/(kg?d) by introgastric (ig) everyday for 7 d. The indices were observed and recorded. Result After given Dragon’s blood,the expression of GSK-3? was decreased (P

Objective To evaluate the efficacy of transversus abdominis plane (TAP) block for postoperative analgesia in the pediatric patients undergoing operation on the inguinal region.Methods Sixty-four pediatric patients of both sexes, aged 1-3 yr, weighing 8.5-23.6 kg, of American Society of Anesthesiologists physical status Ⅰ , undergoing elective unilateral high ligation of the hernia sac or high ligation of the processus vaginalis, were equally randomized into either intravenous analgesia group (group VA) or TAP block group (group TAP).In group TAP, after induction of anesthesia, the pediatric patients received ultrasound-guided TAP block with 0.2％ ropivacaine 1 ml/kg injected locally.After the laryngeal mask airway was inserted, the pediatric patients were mechanically ventilated, and then inhaled 2％-3％ sevoflurane for maintenance of anesthesia.In group VA, postoperative analgesia was performed with fentanyl 0.35 μg · kg-1 · h-1 until 24 h after operation.The level of pain was rated using the FLACC pain scale.When FLACC score＞5, fentanyl 0.25 μg/kg was injected intravenously as rescue analgesic.The occurrence of TAP block-related adverse events, time for removal of laryngeal mask airway, and occurrence of fentanyl-related nausea and vomiting, respiratory depression, and emergence agitation were recorded.Results No TAP block-related adverse events were observed in group TAP, and no respiratory depression was found in the two groups.Compared with group VA, the time for removal of laryngeal mask airway was significantly shortened, and the requirement for rescue analgesics and incidence of vomiting and emergence agitation were decreased in group TAP (P＜ 0.05).Conclusion TAP block with 0.2％ ropivacaine 1 ml/kg provides good efficacy for postoperative analgesia with good safety in the pediatric patients undergoing operation on the inguinal region.

Apoptosis ; drug effects ; Arsenicals ; pharmacology ; Cell Line, Tumor ; drug effects ; Drug Resistance, Neoplasm ; drug effects ; Humans ; Oxides ; pharmacology ; Point Mutation

OBJECTIVETo investigate the role of perindopril for prevention and treatment of glucocorticoid-induced osteoporosis (GIOP) in rabbits.

METHODSA total of 45 male New Zealand white rabbits (10 months old, weight 3.0 to 3.5 kg) were randomly divided into 3 groups involving normal control group (muscle injection of saline solution, n = 15, group NC), model group (muscle injection of dexamethasone, n = 15, group GIOP), and treatment group (muscle injection of dexamethasone combined with oral perindopril, n = 15, group GIOP+ACEI). All rabbits put to death after 12 weeks' treatment. The changes of bone mass and strength were observed and analyzed by bone histomorphology, biomechanics, metabolic bone related serological indexes and mRNA expression.

RESULTSAt 12 weeks, the analysis of bone histomorphology and biomechanics results showed that the bone mass and bone strength of group GIOP were significantly lower than that of group NC (P < 0.05); after perindopril treatment, the bone mass and bone strength of group GIOP+ACEI were higher obviously than that of group GIOP (P < 0.05). Mineralizing surface,mineral apposition rate and serum osteocalcin in group GIOP decreased than group NC; however, osteoclast number, osteoclast surface, eroded surface, and urinary deoxypyridinoline in group GIOP increased than group NC (P < 0.05); these changes were inhibited after perindopril treatment (P < 0.05). Quantitative RT-PCR revealed that after dexamethasone treatment, the ratio of SOST mRNS expression and RANKL/OPG mRNA expression obviously increased than that of group NC (P < 0.05); and Runx2 expression decreased significantly (P < 0.05); while the changes of mRNA expression were improved by perindopril treatment.

CONCLUSIONPerindopril can promote bone formation and inhibit bone resorption to deduce glucocorticoid-induced osteoporosis. This study provides a new method for prevention and treatment of GIOP.

Animals ; Biomechanical Phenomena ; Glucocorticoids ; adverse effects ; Male ; Osteoporosis ; chemically induced ; prevention & control ; Perindopril ; therapeutic use ; Rabbits

Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Pneumonia ; blood ; complications ; diagnosis ; Prognosis ; Retrospective Studies ; Sepsis ; blood ; complications ; diagnosis ; Tumor Necrosis Factor-alpha ; blood

Endophthalmitis ; diagnosis ; drug therapy ; etiology ; Eye Infections, Fungal ; diagnosis ; drug therapy ; etiology ; Humans ; Infant, Newborn ; Infant, Premature ; Male

OBJECTIVE:To explore the effect of nifedipine combined with losartan on the blood pressure and renal function of hypertension complicated with coronary heart disease. METHODS:150 patients with hypertension complicated with coronary heart disease were randomly divided into observation group and control group. Control group was orally treated with Nifedipine sustained release tablet 30 mg,once a day;observation group was additionally treated with Losartan potassium capsules 50 mg,once a day. The treatment course for both groups was 1 month. Systolic blood pressure,diastolic blood pressure,renal function indicators be-fore and after treatment,and incidence of adverse reactions in 2 groups were observed. RESULTS:After treatment,systolic blood pressure and diastolic blood pressure after 3 months was lower than 1 month and lower than before treatment in same group,renal function indicators were significantly lower than before treatment,and observation group was lower than control group,the differ-ences were statistically significant(P<0.05);the incidence of adverse reactions in observation group was significantly lower than control group,the difference was statistically significant(P<0.05). CONCLUSIONS:Nifedipine combined with losartan can well control the blood pressure of hypertension complicated with coronary heart disease,protect renal functions,with good safety.

Objective To observe the shape and the hemodynamic changes of the left heart after transcatheter closure of atrial septal defects(ASD) using Amplatzer occluder.Methods Sixty-five subjects with secondum ASD underwent transthoracic echocardiography(TTE) at pre-operation,48～72 h post-operation and follow-up performed more than 3 months after operation in 45 patients.The dimension of ASD,the parameters of the pulse-wave Doppler spectrums of the blood flow at the aotic valves and inlet of the left ventricle were recorded.At each position,systolic,early diastolic velocities and intervals were measured,the mitral annular movement spectrum was acquired at the lateral site of mitral annulus in the apical four-chamber view,Tei index and Tei_(TDI) index were derived from these parameters.Results All the patients had been implanted one Amplatzer occluder under the guide of echocardiography successfully.The preload of the left ventricular volume in the end of the diastolic period was increased and the low hemodynamic situation of the left heart and systemic circulation were corrected after the operation immediately.Accordingly,at 48～72 h after the operation,the end diastolic diameter,the stroke volume and the ejection fraction of the left ventricle were significantly improved continuously.However,the movement of the posterior wall decreased significantly at 48～72 h post-operation.The peak velocity of systolic period and the early diastolic velocities of the left ventricle were increased,but the ejection time had no changes follow-up.The systolic function of the left ventricle highlighted,the whole diastolic time prolonged after the 3 months of the operation.Tei index,Tei_(TDI) index and the heart rate had no difference between pre-and post-operation.Conclusions The anatomic deformation was corrected by transcatheter closure of ASD with Amplatzer occluder,the pre-load of left ventricular increased and had significant effect on the morphology and hemodynamic situation quickly,the left ventricular systolic and the diastolic function improved.The pre-load change of left heart had no effect on heart rate.Tei index and Tei_(TDI) index were independent of the pre-load and present the heart-self function.

Anemia, Hemolytic, Congenital ; etiology ; Female ; Hepatitis ; complications ; Humans ; Infant ; Syndrome

Objective To evaluate the effect of the high frequency oscillatory ventilation(HFOV) on the treatment of premature neonates with respiratory distress syndrome(RDS).Methods Seventy premature infants with RDS were randomly grouped as HFOV group(n=33) and conventional mandatory ventilation group(CMV group,n=37),based on their fetal age,weight,age,and their clinical condition from Jan.to Sep.in 2009.The blood gas analysis was detected and compared between the 2 groups.Results In HFOV group,the inhaled oxygen concentration,pa(CO2) decreased after treatment for 6 h,which were significantly lower than those at the beginning of the therapy(Pa