Carcinoma, Hepatocellular ; virology ; Genome, Viral ; Hepatitis B virus ; genetics ; Humans ; Liver Neoplasms ; virology ; Mutation

MicroRNAs(miRNAs) are endogenous non-coding RNAs,about 20 nucleotides in length.They play a pivotal role in the regulation of genes involved in diverse biology processes such as cell development,proliferation,differentiation and apoptosis by the translation repression or mRNA degradation.Recent evidence has suggested that miRNA alterations are involved in the initiation and progression of various human cancer including hepatocellular carcinoma(HCC),and miRNA-expression profiling of HCC has identified signatures associated with diagnosis,staging,progression and prognosis.As a novel molecular target,miRNAs holds great promise in diagnosis and biotherapy of HCC.

Circulating nucleic acids (CNAs) are extracellular nucleic acids found in cell-free serum,plasma and other body fluids from healthy subjects as well as in patients. The ability to detect and quantitate specific DNA and RNA sequences has opened up the possibility of diagnosis and monitoring of diseases,especially in the field of cancer. Furthermore,in some recent studies it has been suggested a kind of non-coding RNA-microRNA (miRNA),also exist in cell-free serum and plasma,highlighting the field of using CNAs to diagnose cancer. As a novel tumor marker,tumor-specific circulating miRNAs holds great promise in early diagnosis of cancer.

Hepatocellular carcinoma(HCC),one of the most common malignant tumors,is the main cause of cancer death in China.Early diagnosis of the disease is of great importance.Serum tumor markers have been effective for detecting HCC for a long time.Among those markers,alpha fetoprotein(AFP)is the most widely used one in detecting patients with HCC,but it has limited utility for detecting HCC due to its limited sensitivity and specificity.Searching better markers for HCC has been a re- search focus in recent years.This review introduces many useful markers to supplement AFP for detecting HCC.

Objective To compare the safety of recombinant human tumor necrosis factor-Fc (rhTNFR:Fc) and other DMARDs,in patients with rheumatoid arthritis (RA),ankylosing spondylitis (AS),juvenile idiopathetic arthritis (JIA) or psoriatic arthritis (PsA).Methods Patients who received rhTNFR:Fc 25 mg twice weekly from May 2006 to March 2009 were involved in this open-lable study.Safety assessments were carried out at regular intervals.Results Of the 2014 patients enrolled in the open-label trial,1388,421 and 232 were RA,AS or other diseases,such as JIA and P.sA respectively.Frequent adverse events included injection-site reactions (2.67％),rash (1.87％) and hyperamino transferase (1.80％) in RA patients.Similarly,injection-site reactions (5.23％),hyperaminotransferase (2.38％) and rash (0.71％) were frequent in AS patients.Upper respiratory infection was most frequent among infectious adverse events.There were no reports of patients with serious adverse events,dead case,TB infection and malignancies.Conclusion rhTNFR:Fc has shown a favourable safety profile in Chinese rheumatic disease patients.

Objective To explore and design quality assessment system of optimal medical service based on the BYOD and the SERVQUAL theory.Methods All items of medical service were quantified based on the theory of ERVQUAL to build a service bridge between patients and the medical team and at the same time change the traditional manual approach into a network assessment mode supported by information device.Results With the aid of BYOD technology and theory of SERVQUAL, a set of accurate service quality assessments system were built, and medical personnel could communicate with the patients timely and effectively through the system;to make comprehen-sive analysis of the hospital and to improve the quality of medical services continuously.Conclusion BYOD infor-mation technology combined with the theory of SERVQUAL can help enhance the quality of medical service and patients'satisfaction, improve staff quality and update concept and mode of service, optimize the hospital management and ultimately enhance the level of information management of medical service.

With the completion of human genome,we are entering the functional genomic age.To further reveal the nature of life,glycomics comes into being and becomes a research focus.This review gives a deep insight on the current research progress of glycomics,including the research target,the structural classification and metabolism of sugar chain,the biological significance,research approaches,and the correlation between glycomics and liver diseases.

Early diagnosis of hepatocellular carcinoma(HCC)is still a great challenge in clinical practice.Tumor markers such as alpha-fetoprotein(AFP),liver enzymes,cytokines,and some special glycoproteins,though helpful,are not sensitive and specific enough for early diagnosis of HCC.The establishment of several interesting predictive diagnostic models on liver fi- brosis/cirrhosis suggests that mathematic predictive model,which is developed based on large sample size and follow-up study, might be of higher sensitivity,specificity and feasibility in clinical application.Here we suggest that more attention should be paid to this kind of multi-parameter predictive diagnostic models clinically,so as to improve the early diagnosis of HCC in a more economical and feasible way.

Objective To study the effect of Shenfu injection combined with low dose glucocorticoid on endothelial function and blood coagulation in elderly patients with severe pneumonia. Methods 120 patients with severe pneumonia in our hospital from January 2014 to January 2017 were selected,and randomly divided into treatment group and control group,60 cases in each groups.. All patients were treated with conventional treatment. On this basis, the control group was treated with small dose of glucocorticoid. The treatment group was treated with Shenfu Injection on the basis of the control group. The severity, endothelial function and coagulation function of the two groups were compared at different times after treatment. Results The scores of APACHE and 120 h in the two groups were significantly lower than those in the control group (P<0.05). The score of the treatment group was lower (P<0.05) than that of the control group (96 h). After treatment, the two groups of patients with s E-selectin, V WF, APTT, D-D were significantly decreased, PLT significantly increased, significant difference (P<0.05); compared with the control group, s E-selectin, V WF, APTT, D-D and other indicators of the treatment group decreased and PLT increased even more sharply, there is statistical significance (P<0.05). Conclusion Shenfu injection combined with small dose of glucocorticoid therapy, can alleviate the function and coagulation function in patients with endothelial damage, relieve endothelial dysfunction, improve coagulation status of patients, and has higher application value.

AIM: To investigate the effect of pyrrolidine dithiocarbamate (PDTC), a specific inhibitor of NF-κB on the proliferation and apoptosis of K562 cells and to explore the anti-tumor mechanism of PDTC.METHODS: Trans AM~(TM) NF-κB p65 kit was used to detect the activity of p65 in K562 cells treated by PDTC. The effect of PDTC on the proliferation of K562 cells was measured by WST-1 method. DNA damage was detected by single cell gel electrophoresis (comet assay). The procaspase-3 and activated protein level of caspase-3 were detected by Western blotting.RESULTS: The activity of p65 in K562 cells was inhibited after treated by PDTC (P<0.01). Simultaneously the cell proliferation was significantly inhibited in a dose-and time-dependent manner (P<0.01). The degree of DNA damage in K562 cells treated with PDTC at concentrations of 25 μmol/L, 50 μmol/L or 100 μmol/L was more severe than that in control. The rates of comet cells in the PDTC-treated groups (43.50%, 84.00%, 95.63%) were significantly higher than those in control (9.75%, P<0.01), and it was also dose-dependent. The expression of procaspase-3 and activated caspase-3 protein were detected in the cytoplasm of the K562 cells treated by PDTC by Western blotting.CONCLUSION: NF-κB plays an important role in regulating cell proliferation and apoptosis in K562 cells. PDTC inhibits NF-κB activity and elevates the expression of caspase-3, which is related to increase in cell apoptosis.