Objective:To investigate the method with good sensitivity,specificity,reliability for diagnosis of Chlamydia trachomatis.Methods:Inoculate the nasopharyngeal swabs to detect the Chlamydia trachomatis(CT) with McCoy cell culture and plasmid gene probes labeled with biotin ligase-chain reaction.Then calculate the sensitivity,specificity,positive predicative value(PPV) and negative predicative value(NPV) of both TC and G-LCR respectively.Compare the difference of the two methods.Results:There were 49 positive specimens and 344 negative by enlarged gold standard.There was significant difference with cell culture,G-LCR-PAGE,G-LCR-ELISA by two-related-samples ? 2 tests( P

BACKGROUND: Researchers in China and abroad have done a lot of experiments to study the bond strength of dentine adhesives from generation one to six,which have received satisfied results. However,there are still no reports about the bond strength of the seventh generation adhesive (Adper EasyTM one). OBJECTIVE: To evaluate the bond strength of the Adper EasyTM one to normal dentine and caries-affected dentine,and to compare the results with total-etching adhesives. METHODS: A total of 12 healthy posterior teeth were randomly divided into group A and B; 12 posterior teeth with chronic occlusal caries were divided into group C and D. Adper EasyTM one was applied for group A and C,while Single bond 2 for group B and D. The modes of group A,B,C,D were subjected to microtensile bond strength test. Interfacial morphologies were analyzed by Stereo-Microscopy. RESULTS AND CONCLUSION: The microtensile bond strength of group A and B was (21.84±3.98),(27.10±4.85) MPa,which was (16.44±3.46) and (21.48±4.85) MPa in the group C and D. The differences between group A and B,group C and D,group A and C,as well as group B and D were statistical significant (P < 0.05). Failures mostly occur along the resin-dentine interface. The total-etching adhesives performed more effectively to both normal dentine and caries-affected dentine than Adper EasyTM one. For the same adhesive,the healthy dentine yielded higher bond strength than the caries-affected dentine.

To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cell (HKC), in vitro cultured HKC cells were divided into 3 groups: negtive control, low dose CTGF-treated group (rh CTGF, 2.5 ng/ml) and high dose CTGF-treated (rhCTGF, 5.0 ng/ml). Then the expression of alpha-smooth muscle actin (alpha-SMA) were assessed by indirect immuno-fluorescence, and the percentage of alpha-SMA positive cells were assessed by flow cytometry. RT-PCR were also performed to examine the mRNA level of alpha-SMA. Upon the stimulation of different concentrations of rhCTGF, the expression of alpha-SMA were markedly stronger than that in negative controls. The percentages of alpha-SMA positive cells were significantly higher in the stimulated groups than that of negative controls (38.9%, 65.5% vs 2.4%, P<0.01). alpha-SMA mRNA levels were also up-regulated by the stimulation of rhCTGF (P<0.01). These results suggest that CTGF can promote the transdifferentiation of human renal tubular epithelial cells towards myofibroblast (Myo-F).
Actins/metabolism ; Cell Differentiation/*drug effects ; Cells, Cultured ; Epithelial Cells/*cytology ; Immediate-Early Proteins/*pharmacology ; Insulin-Like Growth Factor Binding Proteins/pharmacology ; Intercellular Signaling Peptides and Proteins/*pharmacology ; Kidney Tubules/*cytology

Objective To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cells (HKC).Methods Cultured HKC were divided into 3 groups: (1) negative control; (2) low dose CTGF treated (rhCTGF 2. 5 ng/ml); (3) high dose CTGF treated (rhCTGF,5. 0 ng/ml). Expression of ?-smooth muscle actin (?-SMA) and fibronectin(FN) mRNA were measured by RT-PCR. Indirect immunofluorescence and flow cytometry methods were used to assess the level of intracellular ?-SMA protein. Concentration of FN secreted into the media was determined by ELBA. Results Upon the stimulations of different concentrations of rhCTGF,expression of ?-SMA and FN mRNA increased markedly(P

Angiotensin Receptor Antagonists ; Atrial Fibrillation ; physiopathology ; Heart Atria ; cytology ; physiopathology ; Humans ; Membrane Potentials ; Receptors, Angiotensin ; agonists

OBJECTIVETo study cellular and humoral immune status on prophase of severe hepatitis B (PSHB).

METHODS56 cases of PSHB patients, 40 cases of chronic hepatitis B (CHB) patients and 20 cases of healthy volunteers were enrolled for detection of CD3+, CD4+, CD8+ and CD3-/CD19+ (B cells) lymphocyte subsets in peripheral blood by flow cytometry. Serum IgG and complement C3 was detected by immunoturbidimetry and analyzed statistically.

RESULTSCompared with CHB group and healthy control group, percentage of lymphocyte subsets CD8+ were significantly lower in PSHB group (P < 0.01 or P < 0.05). While the percentage of lymphocyte subsets CD4+ and ratio of CD4+/CD8+ in PSHB group was obviously higher than those in CHB group (P < 0.+01 or P < 0.05). In addition, There was no significant difference on the percentage of B cell and level of serum IgG between PSHB group and CHB group (P > 0.05, while the level of serum complement C3 in PSHB group were significantly lower than those in CHB group and healthy control group (P < 0.01, P < 0.05).

CONCLUSIONPSHB has a certain degree of cellular immune dysfunction, which characterized by cellular immune function hyperfunction and humoral immune suppression.

Adult ; Antibodies, Viral ; immunology ; Complement C3 ; immunology ; Female ; Flow Cytometry ; Hepatitis B, Chronic ; immunology ; Humans ; Lymphocyte Count ; Male ; Middle Aged ; T-Lymphocyte Subsets ; cytology ; immunology ; Young Adult

Apoptosis ; Cell Proliferation ; Gene Expression ; Gene Silencing ; HL-60 Cells ; Humans ; Intracellular Signaling Peptides and Proteins ; genetics ; RNA, Small Interfering ; genetics

Objective To study the effects of CD2-associated protein (CD2AP) on podocyte adhesion and extension ability and to explore its possible mechanism. Methods Conditionally immortalized murine podocyte cell line was cultured in RPMI 1640 medium at 33℃permissive conditions. The podocytes were transfected with CD2AP small interfering RNA (siRNA) and serambing sequences labeled with fluorescein were taken as control. The transfected podocytes were trypsinized and seed into collagen IV coated plates. The relative cell adhesion and cell area were examined 90 min later. Apoptotic rates of CD2AP siRNA transfected podoeytes and different PAN concentrations incubated podoeytes were detected by flow cytometer. The distribution of F-actin was observed under laser scanning confoeal microscope. Nephrin protein expression and its phosphorylation level were examined by immunofluorescence and Western blot. Results The relative ceil adhesion of CD2AP siRNA transfected podocytes was apparently lower than that of control group[(41.72±6.07)% vs (64.46±8.53)%, P<0.05]. The cell area analysis had the similar result. The apoptotic rate of CD2AP siRNA transfected podocytes was significantly higher than that of the controls [(5.73±0.61)% vs (3.26±0.45)%, P<0.05]. 100 mg/L PAN could markedly induce podocytes to apoptosis and impair cell adhesion ability (P<0.05). Nevertheless, no significant difference was found in cell body spreading (P>0.05). The distribution of F-actin in CD2AP depletion podocytes was apparently altered. The expression of nephrin protein and its phosphorylation level was conspicuously descended to some degree (P<0.05). Conclusions CD2AP depletion facilitates podocyte apoptosis and impairs cell adhesion function. Cytoskeleton confusion and nephrin signaling weakness caused by CD2AP depletion may he partly responsible for the decline of cell adhesion and spreading.

Child, Preschool ; Humans ; Male ; Nevus, Blue ; pathology ; Skin Neoplasms ; pathology