The eye is a common target organ of the allergy, and allergic conjunctivitis is the most common of eye diseases. Clinical manifestations of allergic conjunctivitis are acute bilateral red, itchy, and watery eyes. The presence of conjuncitival eosinophilia may be consided to be a diagnostic indicator of allergic conjunctivitis. ECP is a quantifiable toxic product secreted by activated cosinc phils. VCAM-1 promotes adhesion of leukocytes to endothelium in vitro and may promote imflammation in vivo. The objective of this study is to measure eosinophil count, ECP and sVCAM-1 levels of tears and sera in patients with acute allergic conjunctivitis and normal subjects and to assess the correlation of these mediators with the severity of the disease and the clinical usefulness. Seventeen subjects were selected on the basis of clinical manifestations, history, skin prick test, total IgE. A microcapillary tube was used to collect the tears from the inner canthus, conjunctival epithelia were obtained for eosinophil count by scraping the upper tarsal conjunctiva. The level of ECP was measured by CAP system (Kabi-Pharmacia, Sweden), sVCAM-1 was measured by ELISA (R&D, USA). Serum IgE and eosinophil count were in creased in 10 patients, allergic skin prick test were positive in 11 subjects (D.p: 9, D.f: 8), eosinophilia in conjunctival epithelium were present in 11 subjects (4 patients: > 3/HPF, 7 patients: 1-3/HPF). ECP in tears were increased in patients significantly (12.0+8.0 vs 3.9+3.8 ng/ml, p=0.01), but not in serum (52.5+43.1 vs 28.3+25.9 ng/ml). There is no significant correlation between eosinophil count and ECP in serum and tears (p>0.05, r-=0.19). Serum sVCA-M-1 level is significant different between patients and controls (1916.5+756.0 vs 1147.2+146.1 micro gram/ml, p=0.01), sVCAM-1 is significantly correlated to eosinophil count (p=0.01, r=0.56) and ECP (p<0.05, r=0.65). In conclusion, eosinophil and ECP in tears may be very important role in allergic conjunctivitis and are useful indicators of the disease. The elevation of sVCAM-1 in serum may be interpreted simply as marker of the presence of non-specific inflammation.
Cell Adhesion* ; Child* ; Conjunctiva ; Conjunctivitis, Allergic* ; Endothelium ; Enzyme-Linked Immunosorbent Assay ; Eosinophilia ; Eosinophils* ; Epithelium ; Eye Diseases ; Humans ; Hypersensitivity ; Immunoglobulin E ; Inflammation ; Leukocytes ; Skin ; Tears* ; Vascular Cell Adhesion Molecule-1

The Morel-Lavallée lesion (MLL) is a closed soft-tissue degloving injury, resulting in characteristic hemo-lymphatic fluid collection between the fascia and subcutaneous layers. The MLL was managed routinely with drainage and compression bandages, but sclerotherapy can be used in patients with chronic lesions refractory to first-line therapy. This paper presents a case of extensive recurrent chronic MLL treated with sclerotherapy using Abnobaviscum, which has been used to treat adhesion in malignant pleural effusion.

BACKGROUND: The human homologue of the SC2 gene from a human dermal papilla cell cDNA library has been isolated and designated hSC2. HSC2 protein also shares similarity with 5 -reductase, a protein important in testosterone metabolism. OBJECTIVE: Prior to knowing the functions of hSC2 in dermal papilla, we cloned it and analyzed its relative expression levels in adult tissues and cancer cell lines. METHODS: hSC2 was isolated from low abundant clones in dermal papilla cDNA library using cDNA array hibridization method. Full-length clone was sequenced and we studied its expression in different tissues by Northern blot hybridization. RESULTS: Sequence data reveals a single open reading frame, encoding a putative hydrophobic protein with a calculated molecular weight of 36 kDa. Its deduced amino acid sequences are almost 97.4% identical to t4ose of rat protein. Northern blot hybridization shows that hSC2 cDNA recognizes a 1.35 kb transcript that was expressed in various epithelial and mesenchymal tissues including testis and liver. CONCLUSION: We have cloned and analysed tissue distributions of hSC2. It was interesting that it had homology with 5α-reductase isozymes. Further studies will be needed to understand the involvement of hSC2 in androgen hormone signaling.
Adult ; Amino Acid Sequence ; Animals ; Blotting, Northern ; Cell Line ; Clone Cells ; Cloning, Molecular* ; DNA, Complementary ; Gene Library ; Humans ; Isoenzymes ; Liver ; Metabolism ; Molecular Weight ; Oligonucleotide Array Sequence Analysis ; Open Reading Frames ; Rats ; Testis ; Testosterone ; Tissue Distribution

No abstract available.
Diagnosis* ; Magnetic Resonance Imaging* ; Spinal Stenosis*

No abstract available.
Zygomatic Fractures*

No abstract available.
Bone Marrow Cells/pathology ; Chromosomes, Human, X/*genetics ; Hematologic Neoplasms/*etiology/genetics ; Humans ; Immunophenotyping ; Karyotyping ; Klinefelter Syndrome/*complications/*genetics ; Male ; Middle Aged ; Mosaicism ; Tomography, X-Ray Computed

Chromosomal abnormalities are higher in twin gestations than in the singleton population. Turner's syndrome(gonadal dysgenesis) variety may result from chromosome loss during gametogenesis in either parent or a mitotic error during one of the early cleavage divisions of the fertilized zygote. The vast majority of 45, XO conceptions result in first or second-trimester miscarriage. Fetuses with Tumer's syndrome commonly exhibit posterior nuchal cystic hygromas and generalized edema. Recently we experienced one fetal demise in twin pregnancy. The affected fetus was associated with Turner's syndrome which was diagnosed by amniocentesis and karyotyping. The fetus was associated with cystic hygroma which was antenatally diagnosed by ultrasonogram. The unaffected fetus had normal karyotype and was delivered through cesarean section without any abnormalities. we report this case with brief review of literatures.
Abortion, Spontaneous ; Amniocentesis ; Cesarean Section ; Chromosome Aberrations ; Edema ; Female ; Fertilization ; Fetus ; Gametogenesis ; Humans ; Karyotype ; Karyotyping ; Lymphangioma, Cystic ; Parents ; Pregnancy ; Pregnancy, Twin* ; Turner Syndrome ; Ultrasonography ; Zygote

The objective of this study were to evaluate a correlation between airborne benzene concentrations and urinary muconic acid and to evaluate that between airborne benzene concentrations and urinary phenol concentrations. The high performance chromatographic method was used to measure the muconic acid concentrations in urine samples collected from 69 workers exposed to benzene and 155 non-exposed workers. The time weighted average benzene concentrations in breathing zone of workers were also determined. The physical examination and blood counts (hemoglobin, hematocrit, red blood cell, and white blood cell) were made for both workers exposed to benzene and control group. The results are as follows; 1. The geometric mean of airborne benzene concentrations for all workers was 2.0ppm (0.1-56.9ppm). The mean airborne benzene concentration of workers in adhesive tape manufacturing industry was 12.2ppm (8.3-56.9ppm), which was the highest by type of industry. 2. The geometric mean concentration of muconic acid in urine from male workers exposed to benzene was 3.9 mg/l (0.8-40.8 mg/l), which was significantly higher than that of non-exposed workers. The highest muconic acid concentration was indicated in the workers at the adhesive tape manufacturing industry. 3. There was a significant difference between the mean concentration of muconic acid of workers exposed to airborne benzene concentration of 5-10 pm and that of workers exposed to concentrations below 5 ppm. The urinary muconic acid concentration were correlated with airborne benzene concentration for workers (r=0.85). However there was no significant correlation between urinary phenol concentrations and airborne benzene concentrations. 4. The correlation coefficients between the urinary muconic acid concentration and airborne benzene concentration, urinary phenol concentration, exposure dose(airborne benzene concentration x work duration) and white blood cell were 0.85, 0.74, 0.63 and -0.33, respectively.
Adhesives ; Benzene* ; Environmental Monitoring* ; Erythrocytes ; Hematocrit ; Humans ; Leukocytes ; Male ; Phenol ; Physical Examination ; Respiration

Sequence information from the ribosomal RNA molecule can be used as a molecular clock. Therefore, 16S rRNA sequences have become a widely used tool in bacterial systematics, bacterial typing and studies of the composition of micro-organisms in environmental samples. Consequently, the databases are continuously updated with new 16S rRNA sequence information from different species. This in turn facilitates the identification of new isolates, which is important for the diagnosis of various infectious diseases in research. To development differential identification technique in the Yersinia species, the 16S rDNA for total 17 serotypes of Y. pseudotuberculosis type strains and one of Y. enterocolitica wild strain were amplified by polymerase chain reaction and cloned into pGEM-T vector, partially sequenced and analyzed. The 16S rDNA nucleotide sequence homologies among Yersinia strains were ranged 100% to 93.6%. Phylogenetic tree generated from 16S rDNA sequencing data showed different phyletic line that could be readily separated from each serotypes and other enteric bacterium.
Bacterial Typing Techniques ; Base Sequence ; Classification ; Clone Cells ; Communicable Diseases ; Diagnosis ; DNA, Ribosomal* ; Evolution, Molecular* ; Polymerase Chain Reaction ; RNA, Ribosomal ; Yersinia*