1.Comparative study of eosinophil counts and eosinophil catonic protein(ECP), soluble vascular cell adhesion molecule-l(VCAM-1) in tears and sera in children with allergic conjunctivitis.
Jung Chul SHIN ; Jae Won OH ; Ha Baik LEE
Korean Journal of Allergy 1997;17(3):270-277
The eye is a common target organ of the allergy, and allergic conjunctivitis is the most common of eye diseases. Clinical manifestations of allergic conjunctivitis are acute bilateral red, itchy, and watery eyes. The presence of conjuncitival eosinophilia may be consided to be a diagnostic indicator of allergic conjunctivitis. ECP is a quantifiable toxic product secreted by activated cosinc phils. VCAM-1 promotes adhesion of leukocytes to endothelium in vitro and may promote imflammation in vivo. The objective of this study is to measure eosinophil count, ECP and sVCAM-1 levels of tears and sera in patients with acute allergic conjunctivitis and normal subjects and to assess the correlation of these mediators with the severity of the disease and the clinical usefulness. Seventeen subjects were selected on the basis of clinical manifestations, history, skin prick test, total IgE. A microcapillary tube was used to collect the tears from the inner canthus, conjunctival epithelia were obtained for eosinophil count by scraping the upper tarsal conjunctiva. The level of ECP was measured by CAP system (Kabi-Pharmacia, Sweden), sVCAM-1 was measured by ELISA (R&D, USA). Serum IgE and eosinophil count were in creased in 10 patients, allergic skin prick test were positive in 11 subjects (D.p: 9, D.f: 8), eosinophilia in conjunctival epithelium were present in 11 subjects (4 patients: > 3/HPF, 7 patients: 1-3/HPF). ECP in tears were increased in patients significantly (12.0+8.0 vs 3.9+3.8 ng/ml, p=0.01), but not in serum (52.5+43.1 vs 28.3+25.9 ng/ml). There is no significant correlation between eosinophil count and ECP in serum and tears (p>0.05, r-=0.19). Serum sVCA-M-1 level is significant different between patients and controls (1916.5+756.0 vs 1147.2+146.1 micro gram/ml, p=0.01), sVCAM-1 is significantly correlated to eosinophil count (p=0.01, r=0.56) and ECP (p<0.05, r=0.65). In conclusion, eosinophil and ECP in tears may be very important role in allergic conjunctivitis and are useful indicators of the disease. The elevation of sVCAM-1 in serum may be interpreted simply as marker of the presence of non-specific inflammation.
Cell Adhesion*
;
Child*
;
Conjunctiva
;
Conjunctivitis, Allergic*
;
Endothelium
;
Enzyme-Linked Immunosorbent Assay
;
Eosinophilia
;
Eosinophils*
;
Epithelium
;
Eye Diseases
;
Humans
;
Hypersensitivity
;
Immunoglobulin E
;
Inflammation
;
Leukocytes
;
Skin
;
Tears*
;
Vascular Cell Adhesion Molecule-1
2.Sclerotherapy Using Abnobaviscum for the Extensive Recurrent Chronic Morel-Lavallée Lesions - A Case Report -
Joon-Kuk KIM ; Ji-Won JUNG ; Ki-Chul PARK
Journal of the Korean Fracture Society 2020;33(4):222-226
The Morel-Lavallée lesion (MLL) is a closed soft-tissue degloving injury, resulting in characteristic hemo-lymphatic fluid collection between the fascia and subcutaneous layers. The MLL was managed routinely with drainage and compression bandages, but sclerotherapy can be used in patients with chronic lesions refractory to first-line therapy. This paper presents a case of extensive recurrent chronic MLL treated with sclerotherapy using Abnobaviscum, which has been used to treat adhesion in malignant pleural effusion.
3.Molecular Cloning of hSC2 Encoding 5α-reductase-like Protein.
Ho Chul SEO ; Do Won KIM ; Moon Kyu KIM ; Jung Chul KIM ; Sang Lip CHUNG
Annals of Dermatology 2000;12(4):252-258
BACKGROUND: The human homologue of the SC2 gene from a human dermal papilla cell cDNA library has been isolated and designated hSC2. HSC2 protein also shares similarity with 5 -reductase, a protein important in testosterone metabolism. OBJECTIVE: Prior to knowing the functions of hSC2 in dermal papilla, we cloned it and analyzed its relative expression levels in adult tissues and cancer cell lines. METHODS: hSC2 was isolated from low abundant clones in dermal papilla cDNA library using cDNA array hibridization method. Full-length clone was sequenced and we studied its expression in different tissues by Northern blot hybridization. RESULTS: Sequence data reveals a single open reading frame, encoding a putative hydrophobic protein with a calculated molecular weight of 36 kDa. Its deduced amino acid sequences are almost 97.4% identical to t4ose of rat protein. Northern blot hybridization shows that hSC2 cDNA recognizes a 1.35 kb transcript that was expressed in various epithelial and mesenchymal tissues including testis and liver. CONCLUSION: We have cloned and analysed tissue distributions of hSC2. It was interesting that it had homology with 5α-reductase isozymes. Further studies will be needed to understand the involvement of hSC2 in androgen hormone signaling.
Adult
;
Amino Acid Sequence
;
Animals
;
Blotting, Northern
;
Cell Line
;
Clone Cells
;
Cloning, Molecular*
;
DNA, Complementary
;
Gene Library
;
Humans
;
Isoenzymes
;
Liver
;
Metabolism
;
Molecular Weight
;
Oligonucleotide Array Sequence Analysis
;
Open Reading Frames
;
Rats
;
Testis
;
Testosterone
;
Tissue Distribution
5.A comparison of computerized tomography myelo-enhanced computerized tomography and magnetic resonance imaging in diagnosis of spinal stenosis.
Se Il SUK ; Choon Ki LEE ; Ki Tack KIM ; Won Jung KIM ; Chul Won HA
The Journal of the Korean Orthopaedic Association 1991;26(1):334-343
No abstract available.
Diagnosis*
;
Magnetic Resonance Imaging*
;
Spinal Stenosis*
6.Open reduction of zygomatic fracture using frontotemporal incision.
Chul Ho JANG ; Won Ki WANG ; Ie Dong KIM ; Jung Hwan CHO ; Jung Hun LEE ; Sang Won YOON
Korean Journal of Otolaryngology - Head and Neck Surgery 1992;35(4):525-532
No abstract available.
Zygomatic Fractures*
8.The Effects of Dehydration, Preservation Temperature and Time on the Hair Grafts.
Jung Chul KIM ; Sung Joo HWANG ; Jung Ju LEE ; Byung Min OH ; Seok Jong LEE ; Do Won KIM ; Jung Chul KIM ; Moon Kyu KIM
Annals of Dermatology 2002;14(3):149-152
BACKGROUND: Careful manipulation of hair grafts is essential for a good yield of transplanted hair. OBJECTIVE: The aim of this study was to evaluate some of the factors responsible for poor graft yield, such as dehydration of the graft and the temperature and duration of preservation. METHODS: First, for the dehydration study, isolated single hair follicles were left on dry gauze for 0, 5, 10, 20, and 30 minutes at room temperature. Secondly, to evaluate the effect of preservation temperature and time on the hair graft, follicles were preserved in saline for 5 minutes as a control, then for 6, 24, and 48 hours both at room temperature and at 4℃, respectively. Viability of preserved follicles was judged based on organ culture. RESULTS: Elongation of hair folliciles was seen in 96%, 94%, 94%, 83%, and 68% for 0-, 5-, 10-, 20-, and 30-minute air-exposed groups, respectively. Survival was seen in 95%, 92%, 40% and 34% at room temperature and 96%, 94%, 76% and 50% at 4℃ for follicles preserved in saline for 5 min (control), then for 6, 24, and 48 hours, respectively. CONCLUSION: We suggest that, alone with careful manipulation of hair units, high survival can be achieved with the avoidance of graft dehydration and preservation of the grafts at low temperatures if the operation time extends for more than 6 hours.
Dehydration*
;
Hair Follicle
;
Hair*
;
Organ Culture Techniques
;
Transplants*
9.A Comparative Analysis of Cervical Pap Smears Prepared by Conventional and ThinPrep Method.
Yeon Hwa LA ; Gyung Chul JO ; Sung Tae HAN ; Suk Hee JUNG ; Jung Rae SEO ; Woo Chul JUNG ; Sung Won LEE ; Yong JO ; Eui Sun RO
Korean Journal of Obstetrics and Gynecology 2000;43(8):1450-1458
No abstract available.
10.Development of Technology for Environmental Assessment and Biological Monitoring of Workers Exposed to Benzene.
Chul Whan CHA ; Kwang Jong KIM ; Jung Chul KIM ; Nam Won PAIK
Korean Journal of Occupational and Environmental Medicine 1994;6(1):122-133
The objective of this study were to evaluate a correlation between airborne benzene concentrations and urinary muconic acid and to evaluate that between airborne benzene concentrations and urinary phenol concentrations. The high performance chromatographic method was used to measure the muconic acid concentrations in urine samples collected from 69 workers exposed to benzene and 155 non-exposed workers. The time weighted average benzene concentrations in breathing zone of workers were also determined. The physical examination and blood counts (hemoglobin, hematocrit, red blood cell, and white blood cell) were made for both workers exposed to benzene and control group. The results are as follows; 1. The geometric mean of airborne benzene concentrations for all workers was 2.0ppm (0.1-56.9ppm). The mean airborne benzene concentration of workers in adhesive tape manufacturing industry was 12.2ppm (8.3-56.9ppm), which was the highest by type of industry. 2. The geometric mean concentration of muconic acid in urine from male workers exposed to benzene was 3.9 mg/l (0.8-40.8 mg/l), which was significantly higher than that of non-exposed workers. The highest muconic acid concentration was indicated in the workers at the adhesive tape manufacturing industry. 3. There was a significant difference between the mean concentration of muconic acid of workers exposed to airborne benzene concentration of 5-10 pm and that of workers exposed to concentrations below 5 ppm. The urinary muconic acid concentration were correlated with airborne benzene concentration for workers (r=0.85). However there was no significant correlation between urinary phenol concentrations and airborne benzene concentrations. 4. The correlation coefficients between the urinary muconic acid concentration and airborne benzene concentration, urinary phenol concentration, exposure dose(airborne benzene concentration x work duration) and white blood cell were 0.85, 0.74, 0.63 and -0.33, respectively.
Adhesives
;
Benzene*
;
Environmental Monitoring*
;
Erythrocytes
;
Hematocrit
;
Humans
;
Leukocytes
;
Male
;
Phenol
;
Physical Examination
;
Respiration