BACKGROUND: Basal cell carcinoma(BCC) is the most common tumor of light-skinned people. Most of them are relatively innocuous, but some may cause extensive tissue destruction, and result in death by local infiltration of vital structure or metastatic disease. The bcl-2 is a protooncogene involved in tumorigenesis by blocking apoptosis or programmed cell death. OBJECTIVE: To investigate whether there was a difference in bcl-2 expression in different histopathological types of BCC. Method: We performed immunohistochemical staining by using a monoclonal mouse anti-human bcl-2 oncoprotein in 28 BCC(nodular: 7 cases, micronodular; 3 cases, adenoid; 1 case, infiltrative; 9 cases, and mixed; 8 cases). Result: All 28 BCC expressed bcl-2, and the degree of staining was different according to histopathological types; poor prognostic micronodular(100%) and infiltrative(73.3%) types were relatively strong, and nodular(66.7% ) and adenoid(75.0%) types were moderate. Conclusion . These results suggest that the degree of bcl-2 expression in BCC seems to be dependent to the histopathological types together with clinical behavior.
Adenoids ; Animals ; Apoptosis ; Carcinogenesis ; Carcinoma, Basal Cell* ; Cell Death ; Mice

Condyloma acuminatum occurs in perianal and genital area by infection of human papillomavirus (HPV) and appeais as fairly soft verrucous papules or a cauliflower-like mass. So far 60 HPV types have been identified. Among them, condyloma acuminatum is predomi-nantly associated with HPV 16, 18 and rarely with 16, 18. HPV 16, 18 is regarded as a high-risk HPV infection because of the association of HPV 16, 18 with Bowens disease, cervical cancer, and anogenital cancer. In additioin, a significant appearance of cervical anaplastic lesions is preceded by condyloma acuminatum and a high prevalence of cervical dysplasia has been found in female consorts of men with geriital warts. Malignant transformation has been observed in rare incidences of condyloma acumina a on the external genitalia. We present a case of condylc ma acuminatum showing malignant transforrnation. The patient also has uterine cervical carcinonia. We detected HPV 16 in the lesion using polymerase chain reaction.
Bowen's Disease ; Female ; Genitalia ; Human papillomavirus 16 ; Humans ; Incidence ; Male ; Polymerase Chain Reaction ; Prevalence ; Uterine Cervical Neoplasms ; Warts

BACKGROUND: The warts may promptly return despite being treated with a myriad of destructive methods. The most successful treatment has been the intralesional injection of bleomycin sulfate, but unplessant side effects caused by the difficulty of infiltrating only the wart tissue have limited its use. OBJECTIVE: Our purpose was to determine the effects of bleopunciure technique in the treatment of warts. METHODS: We performed a multiple puncture technique using a 25 gauge hypodermic needle to introduce bleomycin hydrochloricle into warts. RESULTS: This technique resulted in elimination of 91.4% of a random series on 105 warts after a single treatment. Recurrence and side effects were not observed during a 1 year follow-up period. CONCLUSION: We experienced a superior technique for introducing Heomycin into the wart, which has resulted in the cure of a signficant number of warts following a sing treatment.
Bleomycin ; Follow-Up Studies ; Injections, Intralesional ; Needles ; Punctures ; Recurrence ; Warts*

BACKGROUND: The relationship and differentiation among various dendritic cells of the dermis are unclear. Recently it has hecome possible to identify different subpopulat,ions of dermal dendritic cells using anti-CD34 and anti-factor XIIIa antibodies. OBJECTIVE: To elucidate which cell types the fibrous dermal turnors consist of we compared the staining patterns of these antibodies as well as of anti-Mac 387 antibody which are labeled as inflammatory cells of the monocyte-macrophage lineage. METHODS: Tumors studied included dermatofibrosarcoma protuberans(DFSP, n=2), dermato-fibroma(n=22), neurofibroma, n=27), acrochordon(n=15), keloid, hypertrophic scar(n=10), juvenile xanthogranuloma(n=1, and malignant fibrous histiocytoma (MFH, n=1). We performed immunoperoxidase staining(AUSC technique) with polyclonal anti FXIIIa antibody, monoclonal anti-CD34 antibody, and monoclonal anti-Mac 387 antibody on the formalin-fixed-paraffin-embedded tissue specimens of these fibrous tumors. The intensity of staining was graded as negative, weakly staining, or strongly stainiring. RESULTS: FXIIIa reactivity was strongly present in dendritic and spindle-shaped cells of all dermatofibromas and some nurofibromas(11 of 27 specimens), but absent from the other fibrous tumors. Among these tumors, one of the two DFSPs was uniquely expressed CD34. Dendritic and spindle-shaped cells within tiese tumors were MAC 387 negative. In inflammatory conditions, variable numbers of MAC 38 positive cells were observed, corresponding to histiocytes and mac-rophages, but the labeling of ipithelioid cells and multinucleated foreign body giant cells were variable. CONCLUSION: The findings of significant numbers of FXIIIa positive cells in dermal fibrous tumors studied suggest that thet may be diagnostic utility associated with the use of this antit)ody. In addition, CD 34 expression by the tumor cells can be an extremely useful marker in establishing a definitive diagnosis of IFSP.
Antibodies ; Dendritic Cells ; Dermatofibrosarcoma ; Dermis ; Diagnosis ; Factor XIIIa* ; Giant Cells, Foreign-Body ; Histiocytes ; Histiocytoma, Benign Fibrous ; Histiocytoma, Malignant Fibrous ; Keloid ; Langerhans Cells ; Neurofibroma

No abstract available.
gamma-Globulins* ; Purpura, Thrombocytopenic, Idiopathic*

No abstract available.
Child* ; Humans ; Purpura, Thrombocytopenic, Idiopathic*

No abstract available.
Anemia* ; Child* ; Hospitalization* ; Humans

DNA isolated from human tumor cells can induce malignant transfarmation of tissue culture cells. The DNA is then called an oncogene. Its protein produets have been detected in animal and human tumors and are considered to play a significant role in carcinogenesis. In order to evaluate whether the oncogenes are involved in development of tumors of epidermis and whether they could be used as tumor markers, immunoperoxidase staining was performed for the ras product in sections of squamous cell carcinoma, Bowen's disease, actinic keratosis, keratoacanthoma and seborrheic keratosis. Three cases of sgamous cell carcinoma showed 10~20 positive cells per high power field(HPF). Three cases of Bowen's disease revealed 1-9 positive cells per HPF, whereas the actinic keratosis 1~9 or no positive cells per 10 HPF in all three cases. The keratoacanthoma and seborrheic keratosis showed 1~9 or no positive cells in all observed cases. The positive staining was observed in the cytoplasm. The increasing positivity in parallel with the increase of malignant potential strongly suggests that the ras oncogene is closely related to development of epidermal malignancy and also point out the possibility of ras as a cancer marker.
Animals ; Bowen's Disease ; Carcinogenesis ; Carcinoma, Squamous Cell ; Cytoplasm ; DNA ; Epidermis* ; Genes, ras* ; Humans ; Keratoacanthoma ; Keratosis, Actinic ; Keratosis, Seborrheic ; Oncogenes ; Biomarkers, Tumor

Previous observations have demonstrated that fibronectia (FN) is deposited high abundance in basal cell carcinoma (BCC) stroma. Since FN is a glycoprotein which promotes interaction between cells and extracellular matrix, it may been important component of slowly expansile carcinoma such as BCC. Furthermore, BCCs aggressiveness varies with it's histologic appearance. The authors investigated distribution of plasma FN (pFN) in 8 BCC cases with various histologic types (3 nodulocystic type, 2 infiltrative type, 2 keracotic type, 1 adenoid type) and 4 squamous cell carcinomas (SCC), which have a more aggrcisive biologic behavior than BCC using immunohistochemical staining. In normal skin, pFN was strongly positive at the clenzoepidermal junction and perivascular area with a linear pattern, and in the papillary dermis with a reticular pattern, and was weakly positive in the reticular dermis. In BCC, pFN was strongly positive in nodulocystic BCC, with the highest concentration along the periphery of the tumor island in a filamentous pattern, .hereas, it was moderately positive in adenoid, keratotic, infiltrative BCC stroma. In SCC, pFN was weakly positive around tumor the margins with a thin, coarse deposition These results show that with epidermal tumors, the degree of the pFN expression in stroma is propotional to their aggressiveness.
Adenoids ; Carcinoma, Basal Cell* ; Carcinoma, Squamous Cell ; Dermis ; Extracellular Matrix ; Fibronectins* ; Glycoproteins ; Plasma* ; Skin

Bowens disease is generally regarded as a premalignant dermatosis. If untreated, 3% to 5% of patients may develop squamous cell carcinoma. However, sebaciou carcinoma arising from Bowens disease is very rare. We presented a case of quarnous cell carcinoma and sebaceous circ s disease in a 68 year-old male. He had multiple bowenoid skin lesi nsties. A bean-sized nodule as developed on the bowenoid lesion of the he had a large yellow crust.ed exudative tumor on the Rt. thigh. We took a biopsy specimen of these three discrete lesions. The nu lipid stain of frozen section revealed Bowens disease, squarnous concllnoma arising from Bowen on the trunk and extremi Rt. lower abdomen. Almost of routine histology and carcinoma, and sebaceous carcinoma, respectively.
Abdomen ; Aged ; Biopsy ; Bowen's Disease ; Carcinoma, Squamous Cell* ; Frozen Sections ; Humans ; Male ; Skin ; Skin Diseases ; Thigh