This study was designed for the evaluation of the mean proportions and range of individual difference of muscle fiber types in human Gastrocneminus, Soleus, Peroneus longus and Tibialis anterior muscles, respectively. Flash-frozen muscle sections obtained from 15 cadavers were stained for H&E, trichrome, PAS, regular & reversed myosin ATPase, succinic dehydrogenase (SDH), acid phosphatase (Ac-Pase) and alkaline phosphatase (Alk-Pase). The results obtained were as follows. 1) Differentiation of muscle fiber types was not evident in H&E and trichrome stained specimen. Three types of muscle fibers were, however, evident in PAS-stained sections according to the degree of positivity in observed skeletal muscles. 2) Two fiber types (Types I and II) were only differentiated and the predominant muscle fiber type was type I by regular myosin ATPase reaction in Gastrocnemius, Soleus, Peroneus longus and Tibialis anterior muscles. 3) Three muscle fiber types (Type I, IIa and IIb) were, however, differentiated and the predominant muscle fiber type was type IIa, contrary to patterns of regular myosin ATPase in skeletal muscles except for Soleus muscle by reversed myosin ATPase stains. Only two muscle fiber types (Type I and II) and predominant type I fibers were shown in Soleus muscle. 4) SDH stains showed three types of muscle fiber except Soleus muscle. The mean proportions(%) of type 1 fibers were 48.0% in Gastrocnemius, 62.3% in Soleus, 40.8% in Peroneus longus, and 39.8% in Tibialis anterior muscles, respectively. 5) The mean proportions (%) of muscle fiber types in human skeletal muscles were markedly different, according to individuals and various enzyme histochemical stains. 6) The size of muscle fibers was relatevely small in type I fibers compared with type IIb. 7) Evaluation for the combination patterns of various enzyme histochemical activities showed that the common muscle fiber types (Type I, IIa and IIb) were markedly reduced in the mean proportions and unusual rare muscle fibers predominant. This predominance of unusual muscle fiber types and marked discrepancy in muscle fiber types between regular and reversed myosin ATPase seemed to be ascribed to decreased muscle enzyme activities following postmortem period.
Humans

To search the effective antisense oligonucleotide that inhibit the growth of Herpes simplex virus type 1 (HSV-1), six kinds of phosphorothioate oligodeoxynucleotides (S-ODNs) were synthesized and the antiviral activity was assessed by measuring cytopathic effect in Vero cells infected with HSV-1. Of the three dodecamer S-ODNs, cornplernentary to the translation initiation site of IE2 (AS2) and scrambled S-ODN (AS1) showed more significant antiherpetic activity than AS4 complementary to the IE4. Accordingly, the antiviral effect of dodecamer S-ODN was not specific. In contrast to the no inhibitory effect of sense strand S-ODN of ICP8 (AS6), two S-ODNs complementary to the translation initiation site of ICP8 (AS3) and that of IE1 (AS5) showed potent antiherpetic activity assessed in vitro HSV-1 virus yield assay. Antiherpetic effect of AS3 was decreased in proportion to the addition of AS6. The synthesis of viral protein ICPS and IE1 were inhibited in AS3 and AS5 treated HSV-1 infected Vero cells, respectively. These findings suggest that antiherpetic effect of AS3 is specifically mediated by targeting ICPS. S-ODNs had no effect on Vero cell viability. The data suggest that the 19-mer S-ODNs may be effective in antiviral chemotherapy.
Drug Therapy ; Herpes Simplex* ; Herpesvirus 1, Human ; Oligodeoxyribonucleotides ; Phosphorothioate Oligonucleotides* ; Simplexvirus* ; Vero Cells

Author take off the pelvic bone from the cadaver and made the accruate reconstructed the pelvic bone as well as normal bone. Then author setted the pelvic bone into the specialized acrylic globe as the normal person erect position. For the acetabular posterior wall, projected the radiologic beam from the 0°to 45°cephalad direction and then pelvic bone rotated internally 0°~45°, each section is 5°. For acetabular anterior wall, projected the radiologic beam from 0° to 45° caudal direction and pelvic bone rotated externally 0°~45°, each section is 5° too. From the pelvic bone experiments, author get on the good radiologic angle for acetabular anterior and posterior wall. Ane then make the radiologic projection for normal person as the pelvic experiment. In pelvic bone model experiment, For acetabular anterior wall and anterior colum, external rotation 20°~30° caudal tilting 30°~45° angle projection has good visualization. For acetabular posterior wall and posterior column (including anterior column partially), internal rotation 15°~20° cephalad tilting 15°~30°angle projection has the good visualization. In normal person experiment, For acetabular anterior wall and anterior column, external rotation 20°caudal tilting 30°angle projection shows the good visualization. For acetabular posterior wall and column (including anterior column partially) internal rotation 20°cephalad tilting 30°angle projection shows good visualization. From the pelvic bone model and normal person experiments, the following conclusion are obtained. 1. For posterior wall and posterior column(including anterior column partially), internal rotation 20°, cephalad tilting 30°view is good. 2. For anterior wall and anterior column, external rotation 20°, caudal tilting 30°view is good.
Acetabulum ; Cadaver ; Humans ; Pelvic Bones

BACKGROUND: Long-term oral itraconazole and terbinafine are widely used in the treatment of onychomycosis. Accelerated nail growth in patients with itraconazole has been described in several reports. However, there has been no report regarding the effect of antifungal agents on cultured nail matrix cells(NMCs). OBJECTIVE: We applied several antifungal agents on cultured human NMCs and epidermal kera- tinocytes(EKs) to compare the cytotoxicity of several antifungal agents and also to verify possible stimulating effects of itraconazole and 6-hydroxyitraconazole on nail growth. METHODS: To evaluate the effect of antifungal agents, the 3-(4,5-dimethylthiazo1-2-yl) 2,5-diphenyl- tetrazolium bromide(MTT) test, tritiated thymidine incorporation test, and lactic dehydrogenase(LDH) leakage test were used. RESULTS: Dose dependent decreases in cell viability and DNA synthesis, and dose dependent increases in LDH liberation were observed in cultured human NMCs and EKs after exposure to several antifungal agents. The dose-response reaction patterns for NMCs and EKs to antifungal agents were similar. The cytotoxicity potency of several antifungal agents measured by each method were slightly different. Itraconazole and 6-hydroxyitraconazole did not show stimulating effects on cell proliferation in in vitro monolayer cell culture systems. CONCLUSION: These observations suggest that itraconazole appeared less cytotoxic but showed no stimulating effects on nail matrix cell proliferation in vitro. Cultured human EKs as well as NMCs may be useful in evaluating the effects of agents which are involved in nails.
Antifungal Agents* ; Cell Culture Techniques ; Cell Proliferation ; Cell Survival ; DNA ; Humans* ; Itraconazole ; Keratinocytes* ; Onychomycosis ; Thymidine

No abstract available.
Animals ; Brain* ; Rats* ; Serotonergic Neurons*

The injury of the epiphysis or epiphyseal plate produces undesirable effects on the normal growth of the bone, such as bone bridge, growth arrest and angular deformity. Authors performed vascularized fibular epiphysis and epiphyseal plate transplantation in 7 years old girl, who received the excision of the exostosis on distal ulna, followed by progressive varus deformity of right forearm and growth arrest of distal ulna. With follow
Congenital Abnormalities ; Epiphyses ; Exostoses ; Female ; Forearm ; Growth Plate ; Humans ; Methods ; Transplantation ; Ulna

During the last 18 years only a small number of successful replantation of lower extremities severed by trauma have been reported. In this paper we report the first successful case of replantation of completely amputated thigh In Korean literature. A 31-year-old man was admitted to Kyung Hee Unlversity Hospital on February 27th 1976, approximately 1 hour after accident in which the left thigh was completely amputated 15 cm above the knee joint. Circulation was restored 6 hours after amputation through anastomosis of the femoral vein, artery, great saphenous vein, profunda femoris artery and vein. The continuity of sciatic nerve was reestablished by secondary furnicular suture 7 week after injury. About 4 years and 7 months after injury the patient could walk without pain and returned to his occupation. Thre was a almost complete return of motor and sensory function of the posterior tibial nerve.
Adult ; Amputation ; Arteries ; Femoral Vein ; Humans ; Knee Joint ; Lower Extremity ; Microsurgery ; Occupations ; Replantation ; Saphenous Vein ; Sciatic Nerve ; Sensation ; Sutures ; Thigh ; Tibial Nerve ; Veins

It is generally accepted that fractures should be treated by closed methods, however for certain femoral fractures in adults, closed methods yields an unacceptable high incidence of nounlon, malunion, delayed unlon, and disability. For these fractures various methods of open reduction and internal fixation have been recommended, but unfortunately, there are frequent reports of complications and failures. The recently developed association for study of Internal fixation (ASIF) compression plating apparatus, seems to satisfy the basic objectives of internal flxatlons: namely
Adult ; Femoral Fractures ; Femur ; Follow-Up Studies ; Humans ; Incidence ; Joints ; Walking

Antibiotic-acrylic bone cement composites demonstrated antibacterial activity as a result of diffusion of antibiotic from bone cement into surrounding enviroment, accordingly, the use of antibiotic incorporation to bone cement for the prevention and treatment of infection after joint surgery replacement has been adovocated. Early report of clinical success by using antibiotic-acrylic bone cement composites are encouraging. Our authors applied this concept to the treatment of chronic osteomyelitis. Antibiotic contained with bone cement was chosen by sensitivity test on pus culture finding. Seven cases of chronic osteomyelitia treated by using antibiotic-acrylic bone eement composites were .studied in the Department of Orthopaedic Surgery, Kyung Hee University Hospital from July 1979 to March 1981. The follow-up period was from 3 months tc 22 months. The results were as follow: 1. Six cases showed no evidence of recurrence except one by using antibiotic acrylic bone cement composites. 2. The impregantion of antibiotic in acrylic bone cement shall be regarded to be effective in increasing the local antibacterial activity by leaching the antibiotic into surrounding tissue over a period continiously in treatment of chronic osteomyelitis. 3. The use of antibiotic-acrylic bone cement compositea into dead space by saucerization are consid- ered an one such approach to the decrease of dead space. 4. The impregnation of antibiotic in acrylic bone cement is regarded a new concept in treatment of chronic osteomyelitis.
Bone and Bones ; Diffusion ; Follow-Up Studies ; Joints ; Osteomyelitis ; Polymethyl Methacrylate ; Recurrence ; Suppuration

Bone scan measuring bone metastasis were analysed in fifty five patients diagnosed as malignant tumor, seven were primary bone tumor and forty eight were metastatic tumor, who treated in Kyung Hee Hospital from March 1981 to January 1982. The results were as follows: l. In 55 patients, positive bone scan were found in 76.4% of the patients and positive X-ray were found in 56.4%. 2. 7 patients with primary bone tumor showed all positive bone scan, 45 patients with metastatic tumor showed positive bone in 72.9%. 3. Among the patients with metastatic tumor who no clinical symptoms, positive bone scan were found in 59.3% and positive X-ray in 26%. 4. Most common metastatic site was femur in primary bone tumor and vertebra in metastatic tumor. 5. In 48 patients of metastatic tumor, positive bone scan within negative X-ray were found in 56%, negative bone scan within positve X-ray were found in 5%. 6 The lesions showed clod area on bone scan were considered of positive finding as compared with clinical symptom and X-ray finding.
Diagnosis ; Femur ; Humans ; Neoplasm Metastasis ; Spine