1.Human embryos of Carnegie stage 15..
Ho Jeong KIM ; Chul Hoe KOO ; Hyoung Woo PARK
Korean Journal of Physical Anthropology 1991;4(2):127-135
No abstract available.
Embryonic Structures*
;
Humans*
2.Relationship between Therapeutic Response and Plasma HVA/5-HIAA Ratio in Newly Admitted Schizophrenia.
Hoe Duck KOO ; Hae Sook SUH ; Kwang Hyeon KIM ; Chul Eung KIM
Korean Journal of Psychopharmacology 1997;8(1):101-106
Twenty newly admitted acute schizophrenic patients were treated with haloperidol for 6 weeks. HVA and 5-HIAA were sampled at baseline, 3days after initial neuroleptic dose, and after 1, 2, 3, 4, 6 weeks of treatment. Nine patients were classified as responders in this prospective haloperidol treatment trial. They had a score of change in the BPRS total scores of 25% or greater. Eleven patients were classified as nonresponders, based on a score of changes in the BPRS total scores of less than 25%. 1) There was no significant difference in plasma HVA/5-HIAA ratio between responder and non-responder before and after haloperidol treatment. 2) There was no significant correlations between plasma HVA/5-HIAA ratio and BPRS total scores. This study could not support the hypothesis that neuroleptic treatment would be effective by changing dopamine and serotonin function and/or by altering their interaction.
Dopamine
;
Haloperidol
;
Humans
;
Hydroxyindoleacetic Acid
;
Plasma*
;
Prospective Studies
;
Schizophrenia*
;
Serotonin
3.Effect of GABA on the Contractility of Canine Trachealis Muscle.
Cheol Hoe KOO ; Oh Chul KWON ; Eun Mee CHOI ; Kang Youn LEE ; Won Joon KIM
Yeungnam University Journal of Medicine 1994;11(2):314-322
This study aimed to investigate the existence of GABA receptor and the mechanisms of action of GABA and diazepam of the trachealis muscle isolated from dog. Horizontal muscle strips of 2mm×15mm were prepared from canine trachea, and isometric myography in isolated muscle chamber bubbled with 95/5%-O₂/CO₂ at 36℃, at the pH of 7.4 was performed. Muscle strips contracted responding to the electrical field stimulation (ESP) by 2~20 Hz, 20 msec, monophasic square wave of 60 VDC. GABA and diazepam suppressed the EFS-induced contractions to the similar extent, significantly. (p<0.05). Bicuculline, a GABA(A) receptor antagonist blocked both GABA- and diazepam-inhibitions; but DAVA, a GABA(B) receptor antagoinst did not affect either of them. These results suggest than in the canine trachealis muscle, there may be only GABA(A) receptor, and GABA and diazepam inhibit the contractility via GABA(A) receptor.
Animals
;
Bicuculline
;
Diazepam
;
Dogs
;
gamma-Aminobutyric Acid*
;
Hydrogen-Ion Concentration
;
Myography
;
Receptors, GABA
;
Receptors, GABA-A
;
Trachea
4.Serum Hepcidin Level as an Early Marker of Hematopoietic Activity after Allogeneic Peripheral Blood Stem Cell Transplantation.
Jimyung KIM ; Kye Chul KWON ; Sun Hoe KOO
Journal of Laboratory Medicine and Quality Assurance 2013;35(1):1-7
BACKGROUND: Hepcidin plays a central role in the regulation of iron metabolism, and hepatic iron production is stimulated by iron load and inflammation. Recent animal studies have shown that hepcidin levels increase when hematopoiesis is blocked. We aimed to monitor pre- and post-stem cell transplantation hepcidin levels and evaluate its association with hematologic recovery. METHODS: The study group comprised 12 patients with hematologic malignancies (7 with AML, 4 with ALL, and 1 with refractory anemia with excess blasts-2) undergoing allogeneic peripheral blood stem cell transplantation (PBSCT). One day before and 3 days, 1 week, 2 weeks, 4 weeks, and 8 weeks after PBSCT, reticulocyte count and levels of Hb, ferritin, and C-reactive protein were monitored; serum hepcidin-25 was measured by ELISA. RESULTS: The median serum hepcidin-25 levels (ng/mL) were significantly higher until 1 week after PBSCT (103.6, 103.3, and 96.5) than those at 2, 4, and 8 weeks after PBSCT (63.9, 53.9, and 56.6, respectively). The reticulocyte count also significantly increased from 2 weeks after PBSCT. The hepcidin level showed an inverse correlation with reticulocyte count (r=-0.56, P<0.001) and a weak positive correlation with ferritin (r=0.27, P=0.02). At 2 weeks, patients with high hepcidin levels (> or =63.9) tended to demonstrate lower Hb recovery at 8 weeks than patients with low hepcidin levels did (P=0.15), but without any differences in the incidence of complications. CONCLUSIONS: These findings indicate that hepcidin production is associated with erythropoietic activity and that hepcidin level may be used as an early marker of hematopoietic recovery in PBSCT.
Anemia, Refractory
;
Animals
;
Antimicrobial Cationic Peptides
;
C-Reactive Protein
;
Cell Transplantation
;
Ferritins
;
Hematologic Neoplasms
;
Hematopoiesis
;
Humans
;
Incidence
;
Inflammation
;
Iron
;
Organothiophosphorus Compounds
;
Peripheral Blood Stem Cell Transplantation
;
Reticulocyte Count
;
Stem Cell Transplantation
;
Transplants
5.Two Cases of Toxic Shock Syndrome(TSS) in Infants.
Jin Kil PARK ; Hong Ju CHOI ; Hee Tag IM ; Jae Sam KIM ; Hyo Kyung SHIN ; Chul Hoe KOO
Journal of the Korean Pediatric Society 1994;37(6):861-871
Much has been learned of the pathogenesis and pathophysiology of the toxic shock syndrome (TSS) since the initial description in 1978 by Dr. James K, Todd. The clinical illness is defined by the criteria listed in the case definition formulated for epidemiologic studies. With the advent of widespread recognition of TSS, there have been numerous published reports describing the clinical and laboratory findings, primarily in menstruating females. And there have been also reported about six cases in Korea. Moreover, TSS is uncommon in the prepubertal age group and no case report in infant in Korea. We experienced two cases of TSS in infants aged 11/2 yrs and 9 months associated with respiratory infection-pneumonia, pyopneumothorax and localized skin abscess that were confused with Kawasaki disease (KD). The diagnosis was made on the basis of clinical features and laboratory findings, and the cases met the Centers of Disease Control case definition of TSS. And thus we report these cases and review related literatures.
Abscess
;
Diagnosis
;
Female
;
Humans
;
Infant*
;
Korea
;
Mucocutaneous Lymph Node Syndrome
;
Shock, Septic*
;
Skin
6.Epidemiological Characterizations of Class 1 Integrons from Multidrug-Resistant Acinetobacter Isolates in Daejeon, Korea.
Ji Youn SUNG ; Sun Hoe KOO ; Semi KIM ; Kye Chul KWON
Annals of Laboratory Medicine 2014;34(4):293-299
BACKGROUND: Multidrug-resistant (MDR) Acinetobacter spp. acquire antimicrobial agent-resistance genes via class 1 integrons. In this study, integrons were characterized to investigate the antimicrobial resistance mechanisms of MDR Acinetobacter isolates. In addition, the relationship between the integron type and integron-harboring bacterial species was analyzed by using epidemiological typing methods. METHODS: Fifty-six MDR Acinetobacter spp.-A. baumannii (N=30), A. bereziniae (N=4), A. nosocomialis (N=5), and A. pittii (N=17)-were isolated. The minimum inhibitory concentrations (MICs) were determined on the basis of the results of the Epsilometer test (Etest). PCR and DNA sequencing was performed to characterize the gene cassette arrays of class 1 integrons. Multilocus sequence typing (MLST) and repetitive extragenic palindromic sequence (REP)-PCR were performed for epidemiological typing. RESULTS: Class 1 integrons were detected in 50 (89.3%) of the 56 isolates, but no class 2 or 3 integron was found within the cohorts. The class 1 integrons were classified into 4 types: 2.3-kb type A (aacA4-catB8-aadA1), 3.0-kb type B (aacA4-blaI(MP-1)-bla(OXA-2)), 3.0-kb type C (bla(VIM-2)-aacA7-aadA1), and 1.8-kb type D (aac3-1-bla(OXA-2)-orfD). Type A was most prevalent and was detected only in A. baumannii isolates, except for one A. bereziniae isolate; however, type B was amplified in all Acinetobacter isolates except for A. baumannii isolates, regardless of clone and separation time of the bacteria. CONCLUSIONS: Although class 1 integron can be transferred horizontally between unrelated isolates belonging to different species, certain types of class 1 integrons tend to transfer horizontally and vertically among A. baumannii or non-baumannii Acinetobacter isolates.
Acinetobacter/drug effects/isolation & purification/*metabolism
;
Acinetobacter Infections/epidemiology/microbiology
;
Acinetobacter baumannii/drug effects/isolation & purification/metabolism
;
Anti-Bacterial Agents/pharmacology
;
DNA, Bacterial/chemistry/metabolism
;
Drug Resistance, Multiple, Bacterial
;
Humans
;
Integrons/*genetics
;
Microbial Sensitivity Tests
;
Multilocus Sequence Typing
;
Polymerase Chain Reaction
;
Republic of Korea
7.Correlation Between Virulence Genotype and Fluoroquinolone Resistance in Carbapenem-Resistant Pseudomonas aeruginosa.
Hye Hyun CHO ; Kye Chul KWON ; Semi KIM ; Sun Hoe KOO
Annals of Laboratory Medicine 2014;34(4):286-292
BACKGROUND: Pseudomonas aeruginosa is a clinically important pathogen that causes opportunistic infections and nosocomial outbreaks. Recently, the type III secretion system (TTSS) has been shown to play an important role in the virulence of P. aeruginosa. ExoU, in particular, has the greatest impact on disease severity. We examined the relationship among the TTSS effector genotype (exoS and exoU), fluoroquinolone resistance, and target site mutations in 66 carbapenem-resistant P. aeruginosa strains. METHODS: Sixty-six carbapenem-resistant P. aeruginosa strains were collected from patients in a university hospital in Daejeon, Korea, from January 2008 to May 2012. Minimum inhibitory concentrations (MICs) of fluoroquinolones (ciprofloxacin and levofloxacin) were determined by using the agar dilution method. We used PCR and sequencing to determine the TTSS effector genotype and quinolone resistance-determining regions (QRDRs) of the respective target genes gyrA, gyrB, parC, and parE. RESULTS: A higher proportion of exoU+ strains were fluoroquinolone-resistant than exoS+ strains (93.2%, 41/44 vs. 45.0%, 9/20; P< or =0.0001). Additionally, exoU+ strains were more likely to carry combined mutations than exoS+ strains (97.6%, 40/41 vs. 70%, 7/10; P=0.021), and MIC increased as the number of active mutations increased. CONCLUSIONS: The recent overuse of fluoroquinolone has led to both increased resistance and enhanced virulence of carbapenem-resistant P. aeruginosa. These data indicate a specific relationship among exoU genotype, fluoroquinolone resistance, and resistance-conferring mutations.
ADP Ribose Transferases/genetics
;
Anti-Bacterial Agents/*pharmacology
;
Bacterial Proteins/genetics
;
Bacterial Toxins/genetics
;
Carbapenems/pharmacology
;
Drug Resistance, Bacterial/*drug effects
;
Fluoroquinolones/*pharmacology
;
Genotype
;
Humans
;
Microbial Sensitivity Tests
;
Multilocus Sequence Typing
;
Mutation
;
Pseudomonas aeruginosa/*genetics/isolation & purification/pathogenicity
;
Sputum/microbiology
;
Virulence
8.A Case of Polyglandular Autoimmune Syndrome.
Kyung Ah CHOI ; Ji Yeoun KANG ; Chul Hoe KOO ; Wha Mo LEE ; Young Suk JEON
Journal of the Korean Pediatric Society 1998;41(9):1299-1223
When dysfunction of two or more endocrine glands occurs in association with circulating organ specific antibodies directed against the involved glands, the term polyglandular autoimmune (PGA) syndrome is applied. This syndrome is usually classified into three groups. The autoimmune nature of this disease has been based on the presence of lymphocytic infiltration of the affected glands, organ specific autoantibody in serum, cellular immune defects and association with HLA DR/DQ genes. A 12-year-old girl developed PGA syndrome, type III manifesting Grave's disease and insulin-dependent diabetes mellitus. The thyroid microsomal Ab, TSH receptor Ab and pancreatic islet cell Ab were positive. She should be observed for the possible development of adrenal insufficiency and/or other autoimmune disease.
Adrenal Insufficiency
;
Antibodies
;
Autoimmune Diseases
;
Child
;
Diabetes Mellitus, Type 1
;
Endocrine Glands
;
Female
;
Humans
;
Islets of Langerhans
;
Receptors, Thyrotropin
;
Thyroid Gland
9.Adrenal Cortical Adenoma Associated with Spinal Epidural Lipomatosis and Paraplegia.
Il Whan KIM ; Chul Hoe KOO ; Jae Sun PARK ; Sung Uhn BAEK ; Sung Do LEE ; Kyu Ho LEE ; Jae Do KIM ; Bang HUR ; In Sun JUN
Journal of the Korean Pediatric Society 1986;29(7):86-92
No abstract available.
Adrenocortical Adenoma*
;
Lipomatosis*
;
Paraplegia*
10.he Performance of the Newly Released Automated Chemistry Analyzer Rx Imola.
So Youn SHIN ; Kye Chul KWON ; Sun Hoe KOO ; Jong Woo PARK ; Chi Seon KO
Journal of Laboratory Medicine and Quality Assurance 2007;29(2):217-223
BACKGROUND: The Rx Imola (Randox, UK) is newly released bench top - fully automated analyzer based on Window XP software with high-throughput (640 tests per hour with ISE) and continuous random access. We evaluated the performance of Rx Imola for the routine chemistry. METHODS: Repeatability (within-day precision), between-day precision, within-device precision, linearity, recovery rates and correlation were evaluated for 19 items including AST, ALT, ALP, GGT, total bilirubin, calcium, phosphorus, albumin, total protein, BUN, creatinine, glucose, amylase, total cholesterol, triglyceride, HDL, LDH, CK and uric acid. Commercialized quality control materials and patient's sera were used. For correlation study, 747-100 (HITACHI, Japan) and VITROS 950 (Ortho-Clinical Diagnostics, USA) were used as comparative analyzers. RESULTS: Coefficients of variation (CVs) of all items in repeatability and between-day precision study were below 5%. The linearities were statistically acceptable (R2>0.99) for all items. The recovery rates ranged from 95.7 to 105.3%. The comparison study showed high correlation between Rx Imola and 747-100 or VITROS 950. Correlation coefficients of all items were above 0.99 except HDL and albumin. CONCLUSIONS: This study showed satisfactory results in precision, linearity, recovery rates and comparison studies of Rx Imola. It was expected to be useful for routine chemistry analysis and back up, because of high performance, easy handling and small size.
Amylases
;
Bilirubin
;
Calcium
;
Chemistry*
;
Cholesterol
;
Creatinine
;
Glucose
;
Phosphorus
;
Quality Control
;
Statistics as Topic
;
Triglycerides
;
Uric Acid