With a view to investigating the distribution of keratinophilic fungi in soil according to latitudes in south Korea, soil samples were collected at 81 sites in 17 areas in different latitudes across the country and keratinophilic fungi were isolated by means of Vanbreusegherns technique. The results are summarized as follows : 105 strains of keratinophilic fungi were recovered from 56(69.2%) out of 81 samples. Isolated fungi were composed of 41 strains of Trichophyton ajelloi, 24 stains of Chrysosporium species, 20 strains of Microsporum gypseum, and 20 strains of M. cookei. Frequency of isolation of keratinophilic fungi tended to decrease from higher latitudes down to lower latitudes. In areas of higher latutudes, T. ajelloi and M. cookei were isolated more frequently than in lower latitudes. M. gypseem was most frequent in Cheju City, and Chrysosporium species showed an even distribution, Among three areas under different conditions of moisture and shadedness(i.e., shaded wet, shaded dry, and sunny dry areas), frequency of isolation was highest in shaded dry areas(72.2%). T. ajelloi was the most frequent species in all the three areas, especially in shaded wet areas(53.1%). No M. gypsetcm was found in shaded dry areas. As regards distribution at various sites, roadsides and streamsides exhibited the highest frequency of isolation, and hills the lowest. Except for roadsides, T. ajelloi was the most frequent species at all sites, especially in forests. M. gypseum was frequent at sites beside streams M. cookei at streamsides, fields, and hills and Chrysosporim species in gardens, forests, and streets.
Chrysosporium ; Coloring Agents ; Fungi* ; Jeju-do ; Korea* ; Microsporum ; Rivers ; Soil* ; Trichophyton

Recurrent infantile digital fibroma is a peculiar fibrous tumiir that may be single or multiple on fingers and toes of infants and children. We report a 5-year-old gril with recurrent infantile digital fiber oma which was recurrent on the left 5th toe. The lesion had noted at the age of 6 months. and was excised surgically when the child was 3 year old. 2 years after operation, the lesion was recurred in operation site. Microscopically, nodular fibrous mass is attached to flattened,pidermis. The underlying nodule is composed of proliferating fibroblasts surrounded by derse collagenous tissue. We could find eosinophilic inclusion bodies in the cytoplasm of fibro ilast. It was stained pink with H&E, bright red with Massons trichrome and purple with PTAH.
Child ; Child, Preschool ; Collagen ; Cytoplasm ; Eosinophils ; Fibroblasts ; Fibroma* ; Fingers ; Humans ; Inclusion Bodies ; Infant ; Toes

BACKGROUND: The confgurations of the skin surface, as characterized by surface furrows and plateaux, are known to change with ageing. There are many parameters to analyze the effects of ageing processes on the surface configuration. However, there are contradictory results among the authors. OBJECTIVE: The purpose of this study was to compare the sensitivity of various parameters being described in other reports with our new parameters used in evaluating the changes of the skin surface in the agein processes. METHODS: 112 healthy Korean males in 4 age groups participated in this study. Silicon skin replicas were taken from medial and lateral aspects of the forearm. Images of the skin replicas were analyzed by means of a digital image processing system (AIC, GA). Most parameters were calculated and measured in the primary skin axis. Image analyses were porformed by 3 different processes i.e., morphometry, densitometry (optical profilometry), and histograms. RESULTS: In Morphometry, the furrow count in the vertical section (VFC), the coefficient of the developed skin surface (CDSS) and mean width at maximal interception (MWMI) showed a tendencies to decrease with ageing.Irregularity of shadow shape (ISS) showed a tendency to increase with ageing. In Densitometry, the mean surface roughness(Ra) and mean depth of roughness(Rz) showed a tendency to increase with ageing. On histogram analysis, skewness was decreased with ageing but kurtosis did not show any significant changes. CONCLUSION:Ra, Rz, and ISS have shown a tendency to increase in the ageing processes (p<0. 05). VFC, CDSS, MWMI and skewness showed tendencies to decrease with ageing but the results were not statistically significant. The different results between morphometry and densitometry are likely to be due to increrr ent angles in the skin furrows with ageing.
Axis, Cervical Vertebra ; Densitometry ; Forearm ; Humans ; Male ; Silicones ; Skin*

BACKGROUND: Basal cell carcinoma(BCC) is the most common tumor of light-skinned people. Most of them are relatively innocuous, but some may cause extensive tissue destruction, and result in death by local infiltration of vital structure or metastatic disease. The bcl-2 is a protooncogene involved in tumorigenesis by blocking apoptosis or programmed cell death. OBJECTIVE: To investigate whether there was a difference in bcl-2 expression in different histopathological types of BCC. Method: We performed immunohistochemical staining by using a monoclonal mouse anti-human bcl-2 oncoprotein in 28 BCC(nodular: 7 cases, micronodular; 3 cases, adenoid; 1 case, infiltrative; 9 cases, and mixed; 8 cases). Result: All 28 BCC expressed bcl-2, and the degree of staining was different according to histopathological types; poor prognostic micronodular(100%) and infiltrative(73.3%) types were relatively strong, and nodular(66.7% ) and adenoid(75.0%) types were moderate. Conclusion . These results suggest that the degree of bcl-2 expression in BCC seems to be dependent to the histopathological types together with clinical behavior.
Adenoids ; Animals ; Apoptosis ; Carcinogenesis ; Carcinoma, Basal Cell* ; Cell Death ; Mice

Background: Insulin is known to act as second effector that provides potassium transfer from extracellular fluid to intracellular fluid, thus causing hypokalemia and arrythmia. We evaluated the effect of magnesium sulfate treatment on hemodynamics and serum potassium concentration, induced by continuous infusion of regular insulin solution in rabbits. Method: Twenty-four rabbits were divided into four groups for the experiment. Group I was given intravenouse injection of 5 ml of normal saline and continuous dripping of 7.5 U of RI. Group II was pretreated with 30 mg/kg of magnesium sulfate, followed by continuous dripping of 7.5 U of RI. Group III was given 5 ml of normal saline and 15 U of RI. And Group IV was administered 15 U of RI after 30 mg/kg of magnesium sulfate pretreatment. Venouse blood sampling was done before pretreatment, and after 5, 30, 60 and 120 minutes respectively. Heart rate and arterial blood pressure were taken at the same intervals. Result: 1) There was no significant change in serum potassium concentration in groups injected with 7.5 units of RI respectively (groups I and II), regardless of magnesium pretreatment. But in groups injected with 15 units of RI, the group that was not pretreated with magnesium (Groups III) showed a decrease in serum potassium concentration from 3.39+/-0.57 Eq/L to 2.29+/-0.71 mEq/L, while the concentration in the pretreated group (Group IV) decreased more significantly from 3.35+/-0.50 mEq/L to 1.81+/-0.41 mEq/L. 2) Pulse rate did not change significantly in groups that received continuous infusion of 7.5 units of RI (groups I and II) regardless of magnesium pretreatment, but increased significantly in groups injected with 15 units of RI (groups III and IV), pretreated or not. The pretreated group (group IV) had a more significant rise in pulse rate compared with the group that was not pretreated (group III). 3) In all of the groups, those pretreated with magnesium (groups II and IV) and those not pretreated (groups I and III), there was no significant change in systolic and diastolic blood pressures. Nor was arrhythmia detected. Conclusion: The above results indicate that while magnesium sulfate pretreatment at 30 mg/kg with insulin infusion affects serum potassium concentration, has an antiarrhythmic effect, and may induce tachycardia, it does not have any significant effect on blood pressure.
Arrhythmias, Cardiac ; Arterial Pressure ; Blood Pressure ; Extracellular Fluid ; Heart Rate ; Hemodynamics* ; Hypokalemia ; Insulin ; Intracellular Fluid ; Magnesium Sulfate ; Magnesium* ; Potassium* ; Rabbits* ; Tachycardia

No abstract available.
Paraffin* ; Thoracoplasty* ; Tuberculosis, Pulmonary*

Condyloma acuminatum occurs in perianal and genital area by infection of human papillomavirus (HPV) and appeais as fairly soft verrucous papules or a cauliflower-like mass. So far 60 HPV types have been identified. Among them, condyloma acuminatum is predomi-nantly associated with HPV 16, 18 and rarely with 16, 18. HPV 16, 18 is regarded as a high-risk HPV infection because of the association of HPV 16, 18 with Bowens disease, cervical cancer, and anogenital cancer. In additioin, a significant appearance of cervical anaplastic lesions is preceded by condyloma acuminatum and a high prevalence of cervical dysplasia has been found in female consorts of men with geriital warts. Malignant transformation has been observed in rare incidences of condyloma acumina a on the external genitalia. We present a case of condylc ma acuminatum showing malignant transforrnation. The patient also has uterine cervical carcinonia. We detected HPV 16 in the lesion using polymerase chain reaction.
Bowen's Disease ; Female ; Genitalia ; Human papillomavirus 16 ; Humans ; Incidence ; Male ; Polymerase Chain Reaction ; Prevalence ; Uterine Cervical Neoplasms ; Warts

BACKGROUND: The warts may promptly return despite being treated with a myriad of destructive methods. The most successful treatment has been the intralesional injection of bleomycin sulfate, but unplessant side effects caused by the difficulty of infiltrating only the wart tissue have limited its use. OBJECTIVE: Our purpose was to determine the effects of bleopunciure technique in the treatment of warts. METHODS: We performed a multiple puncture technique using a 25 gauge hypodermic needle to introduce bleomycin hydrochloricle into warts. RESULTS: This technique resulted in elimination of 91.4% of a random series on 105 warts after a single treatment. Recurrence and side effects were not observed during a 1 year follow-up period. CONCLUSION: We experienced a superior technique for introducing Heomycin into the wart, which has resulted in the cure of a signficant number of warts following a sing treatment.
Bleomycin ; Follow-Up Studies ; Injections, Intralesional ; Needles ; Punctures ; Recurrence ; Warts*

BACKGROUND: Several keratolytic agents have been used in many dirmatologic conditions such as callus, keratosis palmaris of plantaris, however the evaluation method of the effects of keratolytiic agents has not been good enough in clinical use. OBJECT: We have conducted an investigation to measure the effect of several keratolytic agents using an in vitro model. METHOD: We measured the fegraded protein of keratin by the bui iet method after adding enzymes such as trypsin, pepsin and papain, and irritants, salicylic acid and sodii m lauryl sulfate to the collected callus. RESULT: The order of the keratolytic effect of the enzymes was trypsir >pepsin>papain. It was difficult to detect the keratolytic effect of salicylic acid becaues of color hiidrance and there was an increasing tendency of keratolyti effect of sodium lauryl sulfate, however it was prominent mainly at a high concentration. CONCLUSION: These results suggested that the potency of similar types of keratolytic enzymes such as pepsin, trypsin and papain can be possible. However it was not such a sitable model to check the potency of the keratolytic effect of salicylic acid and the eoncentration tevel may be an important factor for certain kinds of chemicals such as sodium lauryl sulfate.
Bony Callus ; Irritants* ; Keratolytic Agents ; Keratosis ; Papain ; Pepsin A ; Salicylic Acid ; Sodium Dodecyl Sulfate ; Trypsin

No Abstract Available.
Amblyopia*