Tu-Jia medicine is an important part of traditional Chinese medicine (TCM).“San-Yuan doctrine”is the guiding ideology of Tu-Jia medicine, but there is no systematic exposition of its literature origin. This paper ar-gued that“San-Yuan doctrine”of Tu-Jia medicine was originated in the“San-Yuan”of Taoism. Its formation was closely related to the impact of Taoism and Tu-Jia culture, the understanding of human physiology and pathology in Tu-Jia medicine. This paper expounded the origin of“San-Yuan doctrine”, the guiding ideology of Tu-Jia medi-cine and Taoism culture through citing a number of theoretical bases. First of all, the concept of“San-Yuan”in Tu-Jia medicine originated in Taoism. Secondly, legends about the Lord Lao-Zi and Medicine King Bodhisattva in Tu-Jia medicine were closely related with Taoism. Moreover, there existed important links between Tu-Jia medicine and Taoist medicine. For example, both of them are aware of the importance of brain. The reason, I think, is firstly due to the penetration of Taoism in Tu-Jia culture. Taoism culture has a profound influence on Tu-Jia culture. Sec-ondly, the understanding of human physiology and disease in Tu-Jia medicine is closely related to“three”. There-fore, Tu-Jia medicine took“San-Yuan doctrine”as its own guiding ideology. It is of great significance to the study of ethnic minority medicine culture and inheriting its academic thoughts by the study of Tu-Jia medicine origin.

Objective To investigate the effects of Gastrodin on AKT/NLRP3 pathway in BV-2 microglial pyroptosis model.Methods BV-2 microglia were divided into normal control group and model group(1 μg·mL-1 LPS+10 μmol·L-1 Nigericin),Gastrodin group(10,100 μg·mL-1 Gastrodin),inhibitor control group(10 μmol·L-1 LY294002),inhibitor group(100 μg·mL-1 Gastrodin+10 μmol·L-1 LY294002),using 1 μg·mL-1 LPS combination 10 μmol·L-1 Nigericin co intervened to induce pyroptosis of BV-2 microglia.CCK-8 observed the effect of Gastrodin on cell activity,and western blot detected p-AKT,NLRP3,ASC,caspase-1,IL-1β expression,immunofluorescence detection of NLRP3,p-AKT protein expression,Real-time PCR observation of IL-1β mRNA content.Molecular docking was used to determine the binding sites of Gastrodin to p-AKT1/2/3 and NLRP3 proteins.Results Compared with the control group(0 μg·mL-1 Gastrodin),0.1-1000 μg·mL-1 Gastrodin had no significant effect on BV-2 cell activity(P>0.05).In the western blot experiment,compared with the normal group,the expression p-AKT,NLRP3,ASC,caspase-1,IL-1β in model group was significantly increased(P<0.01);Compared with the model group,the expression of p-AKT,NLRP3,ASC,caspase-1,IL-1β in Gastrodin group decreased(P<0.05 or P<0.01).In immunofluorescence,the expression of NLRP3 in model group was significantly higher than that in normal group(P<0.01);Compared with the model group,the expression of p-AKT and NLRP3 in Gastrodin group decreased significantly(P<0.01);Compared with Gastrodin group,the expression of p-AKT and NLRP3 in the inhibitor group decreased(P<0.01).In the Real-time PCR experiment,compared with the normal group,IL-1β mRNA content in model group cells increased significantly(P<0.01);Compared with the model group,IL-1β mRNA content in Gastrodin group cells decreased(P<0.01).In the LDH content test,compared with the normal group,the LDH activity in the model group cells increased significantly(P<0.01);Compared with the model group,the intervention of 100 μg·mL-1 Gastrodin can reduce the LDH activity in the pyroptosis cells(P<0.01).In molecular docking,Gastrodin had the binding ability with AKT1/2/3 and NLRP3,with the binding energy range of(-6.6)-(-6.8),and the binding mode included hydrogen bond,van der Waals force,etc.Conclusion Gastrodin can improve the pyroptosis of BV-2 microglia by inhibiting AKT/NLRP3 pathway.