1.Bronchial foreign body of a 8-month infant: a case report.
Dahong LIAO ; Chuanxin DUAN ; Linghan HUANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(12):568-568
A foreign body was found in left bronchi of a 8-month infant. A fish bone of 26 mm long was pulled out with bronchoscopy under general anesthesia. This kind of surgery done in infant is more challenging than that done in child.
Anesthesia, General
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Bronchi
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Bronchoscopy
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methods
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Foreign Bodies
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surgery
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Humans
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Infant
2.Diagnosis and therapy of congenital cyst of tongue in neonatus and cystic excision.
Liu YANG ; Chuanxin DUAN ; Linghan HUANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(9):430-430
Cysts
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congenital
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diagnosis
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therapy
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Humans
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Infant, Newborn
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Tongue Neoplasms
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congenital
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diagnosis
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therapy
3.Effect of praziquantel on schistosomal ovum granuloma and hepatic fibrosis Ⅰ Effect of praziquantel on schistosomal ovum granuloma in the lung of mice
Yongliang XU ; Hongjun LI ; Yixin HUANG ; Chuanxin YU ; Yousheng LIANG ; Yuyu WU ; Weihong GAO
Chinese Journal of Schistosomiasis Control 1991;0(05):-
Objective To explore the effect of praziquantel on schistosomal ovum granuloma in the lung of mice.Methods Forty-eight mice were divided into 4 groups.Group A:first,the mice were injected with schistosomal ova hypodermicly in abdomen and 10 days later,injected with schistosomal ova intravenously in the cauda;Group B:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel [300 mg/(kg?d)] for 3 days from the last day of the intravenous injection of the ova;Group C:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel(75 mg/kg,B.i.d.)for 5 days weekly until the mice were sacrificed;Group D:the same as Group C but praziquantel was given to the mice from the 29th after the intravenous injection of the ova.Three mice of each group were sacrificed on the 7th,14th,28th,56th day after the intravenous injection of the ova and the lung tissues were fixed with formalin and the slices were HE stained.Fifteen-thirty pieces of schistosomal ovum granuloma were examined and their areas were measured and the mean areas of each group were calculated and compared.Results On the 7th,14th and 28th days after the intravenous injection of the ova,the mean area of schistosomal ovum granuloma in Group C was significantly less than that in Group A,and there was a significant difference between the two groups,P 0.05.On the 56th day,the mean areas of schistosomal ovum granuloma in Group B,C,D were significantly less than that in Group A,all P
4.Expression of Nitric Oxide Synthase (NOS) mRNA in the Gonad of Oncomelania hupensis Cultured in Different Temperature
Kun YANG ; Xiaonong ZHOU ; Chuanxin YU ; Xuren YIN ; Qingbiao HONG ; Yanping ZHANG ; Yixin HUANG
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(03):-
Objective To study the expression of nitric oxide synthase (NOS)mRNA in the gonad of Oncamelania hu-pensis in different temperature. Methods The snails were cultured at temperature of 0℃ , 15 ℃and 25℃for 1 month. The total RNA of each group was extracted with the RNA extraction kit. A pair of degenerate primers was designed from conserved regions of mammalian NOSs, and the expression of NOS mRNA in the gonad was measured by means of reverse transcription polymerase chain reaction (RT-PCR). Results The target genes of NOS were detected in the gonad of snails. The level of expression of snail NOS mRNA in 25℃ group and 0℃group was significantly higher than that in the control group( P 0.05). Conclusion The designation of primers of the snails was validated. The impact of temperature on the expression of snail NOS mRNA was determined, which suggested that NO plays an important role in the physiological and pathological modulation.
5.Effect of praziquantel on schistosomal ovum granuloma and hepatic fibrosis Ⅱ Effect of praziquantel on cells within pulmonary schistosomal ovum granuloma in mice
Yongliang XU ; Hongjun LI ; Yixin HUANG ; Chuanxin YU ; Tiesheng WANG ; Zhongxing WU
Chinese Journal of Schistosomiasis Control 2010;22(2):105-110
Objective To study the effect of praziquantel on cells within sehistosomal ovum granuloma in the lung of mice.Methods Forty-eight mice were divided into 4 groups.Group A:first,the mice were injected with sehistosomal ova hypodermically in abdomen,and 10 days later,injected with schistosomal ova intravenously in the cauda;Group B:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel[300 mg/(kg·d)]for 3 days,one day before the intravenous injection of the ova;Group C:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel(75 mg/kg,B.i.d.) for 5 days weekly until the mice were sacrificed;Group D:the same as Group C but praziquantel was given to the mice from the 29th after the intravenous injection of the ova.Three mice of each group were sacrificed on the 7th,14th,28th,56th day after the intravenous injection of the ova and the lung tissues were fixed with formalin and the slices were HE stained.Twenty-five to thirty pieces of schistosomal ovum granuloma were examined and the neutrophilic granulocytes,eosinocytes,lymphocytes,fibroblasts and macrophages within the ovum granulomas were counted and the mean numbers of them of each group were calculated and compared.Results Compared with Group A,the mean numbers of neutrophilic granulocytes,eosinocytes and macrophages within the ovum granulomas were decreased significantly,and the extend of the increase of fibroblasts reduced significantly in the three groups administered with praziquantel,and especially in Group C.On the 56th day after the intravenous injection of the ova,the mean numbers of neutrophilic granulocytes,eosinocytes and macrophages decreased by 54.4%、87.0% and 23.1%,and the extend of the increase of fibroblasts reduce by 59.4%,respectively in Group C,compared with Group A.The numbers of lymphocytes did not change very much in 4 groups.Conclusion Praziquantel can restrain inflammatory cells and the hyperplasia of fibroblasts within schistosomal ovum granulomas.
6.Bioinformatics study on the hub genes of glomerulopathy in type 2 diabetic nephropathy
Jiao KONG ; Chuanxin LIU ; Baonan MA ; Yining ZHANG ; Jiali ZHOU ; Qian WANG ; Jie ZHANG ; Jianmei HUANG
Chinese Journal of Endocrinology and Metabolism 2021;37(4):274-280
Objective:To identify the hub differentially expressed genes(DEGs)of glomerular pathological changes and potential pathways in molecular process of type 2 diabetic nephropathy(DN)based on bioinformatics technology.Methods:The differentially expressed genes of Gene Expression Omnibus(GEO)dataset GSE96804 in DN and normal kidney tissues were analyzed by R 3.6.2 software. DEGs were further assessed by Gene Ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signal pathway analysis. Subsequently, the hub genes and their associated pathways were analyzed using String 11.0 and Cytoscape 3.7.2 software.Results:A total of 168 DEGs were obtained in the dataset. Among them, seven hub genes were identified, including ALB, FN1, EGF, PTGS2, PLG, KDR, and LOX. Three hub genes, ALB, EGF, PLG, exerted a direct action on glomerulus. GO enrichment analysis of DEGs was mainly manifested in extracellular matrix organization, extracellular structure organization, platelet degranulation and other biological processes, extracellular matrix, secretory granule lumen, platelet alpha granule and other cell components, chaperone binding, copper ion binding, antioxidant activity, and other molecular functions. DEGs mainly regulated metabolic process, which was related to fatty acid degradation signal pathway, exogenous substance metabolism related to CYP enzyme and drug metabolism signal pathway.Conclusion:A bioinformatics analysis of DN from the perspective of glomerulopathy is helpful to understand the potential molecular mechanism of DN and provide reference for further validation.
7.Low-frequency tympanometry in normal neonates.
Zhiqi LIU ; Kun YANG ; Chuanxin DUAN ; Zhiwu HUANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2008;22(10):456-458
OBJECTIVE:
To find the characteristic of tympanogram results obtained from normative neonates 2 - 7 days about low frequency (226 Hz) probe tone, and to determine the normative values for tympanometric variables.
METHOD:
Transient evoked otoacoustic emission (TEOAE) screening were performed by using AccuScreen Pro instrument for 135 neonates. Among them, 105 neonates passed the TEOAE screening in both ears. The 226 Hz probe tone tympanograms and their normative values were obtained from them using GSI-33 middle ear analyzer.
RESULT:
The 226 Hz tympanometric data for the 105 neonates (210 ears) passed the TEOAE screening in both ears showed a double-peaked tympanogram in 202 ears (96.19%), a single-peaked tympanogram in 8 ears (3.81%). For double-peaked tympanogram in 202 ears, the tympanometric normative values was below: the first peak admittance is (0.91 +/- 0.18)mmho, Tpp is about (18.02 +/- 12.26)daPa; the second peak admittance is (1.05 +/- 0.23)mmho, Tpp is about (-35.05 +/- 16.80) daPa; the admittance of notch between the two peaks is (0.74 +/- 2.57)mmho, its pressure is about (0.37 +/- 7.61) daPa. Vec is about (0.50 +/- 0.08)ml.
CONCLUSION
The 226 Hz tympanograms obtained from this cohort may serve as a guide for evaluating middle ear function in neonates.
Acoustic Impedance Tests
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methods
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Acoustic Stimulation
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Evoked Potentials, Auditory, Brain Stem
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Female
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Humans
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Infant, Newborn
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Male
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Neonatal Screening
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Otoacoustic Emissions, Spontaneous
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Reference Values
8.Thymosin Alpha-1 Inhibits Complete Freund's Adjuvant-Induced Pain and Production of Microglia-Mediated Pro-inflammatory Cytokines in Spinal Cord.
Yunlong XU ; Yanjun JIANG ; Lin WANG ; Jiahua HUANG ; Junmao WEN ; Hang LV ; Xiaoli WU ; Chaofan WAN ; Chuanxin YU ; Wenjie ZHANG ; Jiaying ZHAO ; Yinqi ZHOU ; Yongjun CHEN
Neuroscience Bulletin 2019;35(4):637-648
Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems. The immunopotentiator thymosin alpha-1 (Tα1) has recently been reported to have anti-inflammatory and neuroprotective functions in rodents. However, how Tα1 affects inflammatory pain remains unclear. In the present study, intraperitoneal injection of Tα1 attenuated complete Freund's adjuvant (CFA)-induced pain hypersensitivity, and decreased the up-regulation of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in inflamed skin and the spinal cord. We found that CFA-induced peripheral inflammation evoked strong microglial activation, but the effect was reversed by Tα1. Notably, Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter (VGLUT) and down-regulated the vesicular γ-aminobutyric acid transporter (VGAT) in the spinal cord. Taken together, these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microglia-induced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord.