OBJECTIVE To control the nosocomial infection in the whole process of blood collecting on a blood collecting vehicle.METHODS To supervise the technological sanitation of blood collecting environment,increase primary assay of ALT,using laser beam apparatus instead of needle puncture,select the dynamic disinfection cleaning machine instead of classical tri-oxygen or ultraviolet lamp disinfection,and standardize blood collecting procedure and environmental disinfection.RESULTS After 110 thousand times a year of blood collecting on the blood collecting vehicle in Wuhan there were no one nosocomial infection happened.CONCLUSIONS The nosocomial infection in the whole process of blood collecting on a blood collecting vehicle can be prevented by the quality control of environmental condition and improvement of assay installation.