In the present paper,the authors review progresses of researches on the protective effect of acupuncture in resisting acute myocardial ischemia-reperfusion injury in patients and animals from 1) resisting injury of oxygen-derived free radicals,2) reducing intracellular Ca2+ overload,3) relieving dysfunction of vascular endothelial cells of the myocardium,4) regulating immune functional activity and reducing inflammatory injury,5) reducing cardiocyte apoptosis,6) upregulating the expression of heat shock protein(HSP 70) in the myocardium,and 7) regulating activities of multiple cardiovascular protection factors and intracellular signaling pathways.The abovementioned results are mainly obtained from experimental animal studies.For this reason,the authors hold that the coming researches on acupuncture therapy for cardiovascular disorders should pay more attention to its clinical application,for example,controlled blood-pressure decline induced lower perfusion of the coronary artery,cardiac surgery(percutaneous tranluminal coronary angioplasty,intracardiac operation underdirect vision) etc.

Objective To observe the antimutagenic action of sodium selenite and vitamin E on condensates of cooking oil fumes(COF) Methods SCE (sister chromatid exchanges) were observed and contrasted before and after addition of sodium selenite or vitamin E at a certain dose to the culture of human peripheral blood lymphocytes treated by COF Results Sodium selenite added to lymphocytic culture treated by COF at 37 ℃ for 4 hour incubation significantly lowered the frequencies SCEs of lymphocytes and very significant synergistic effects on lowering frequencies of SCEs were observed during adding both sodium selenite(terminal concentration 0 9?10 -7 mol/L) and vitamin E(torminal concentration 0 1% (v/v) to lymphocytic culture treated ty COF for 4 hour incubation Conclusion Combined sodium selenite and vitamin E at certain concentrations in cultured lymphocytes for 4 hour incubation showed very significant synergistic effects on lowering the mutagenicity of lymphocytes induced ty COF.Sodium selenite alone was also shown to be effective in lowering the frequencies of SCEs of human peripheral blood lymphocytes

Objective To explore the effect of platelet-rich plasma (PRP) on the generation of reactive oxygen species (ROS) and the phenotypes of photo-aging fibroblasts.Methods A photoaging cell model by repeating UVB irradiation was treated using appropriate concentration of PRP;Cell morphology and the rate of aging dying were observed under inverted microscope 24 hours later after establishment of the cell model;The expression of ROS between experimental and control group was detected using fluorescence microscope after single UVB irradiation.The relative intensity of fluorescence was analyzed using flow cytometry.Results PRP could ameliorate the large and sprawl appearance of photoaging fibroblasts obviously,reduce the generation of ROS as well as decrease the relative intensity of ROS.Conclusions PRP can decrease the level of intracellular oxidative stress caused by UVB irradiation,reduce the generation of ROS and ameliorate the senescence-like phenotypes of pho toaging fibroblasts.