To investigate the effect and mechanism of intravascular brachytherapy with 192 Ir on expression of type I collagen after angioplasty. Restenosis model of domestic miniswine was employed.After angioplasty, the iliac arteries were randomized to brachytherapy group ( n =6) , which were treated with 20 25Gy of 192 Ir, and control group ( n =18). The target vessels were harvested at the 12 th week and the 24 th week after angioplasty. Immunohistochemistry and in situ hybridization were used to detect proteins of type I collagen, metalloproteinase 1 (MMP 1) and its tissue inhibitor (TIMP 1), and mRNA expression of type I collagen. The results showed that the protein and mRNA of type I collagen, and the ratios of TIMP 1/MMP 1 were lower in brachytherapy group than in control group. The peak of mRNA expression of type I collagen was at the 24 th week in control group and at the 12 th week in brachytherapy group, respectively. It suggested that intravascular brachytherapy with 192 Ir affects the metabolism of extracellular matrix by inhibiting the synthesis of type I collagen and regulating the activities of MMP 1 and TIMP 1.

The 4-layer sphere model of human head was built; the layers from outside to inside represented the scalp, the skull, the CFS and the brain, respectively. The point current source placed on the outmost layer was regarded as the boundary condition, and the method of separation of variables was used to solve the equations. The potential distribution and the current expression were given, and the isopotential-line map and current lines were drawn. The simulation result can be used to analyze head EIT problems.
Algorithms ; Brain ; anatomy & histology ; physiology ; Computer Simulation ; Electric Impedance ; Electrodes ; Electromagnetic Phenomena ; Head ; physiology ; Humans ; Image Interpretation, Computer-Assisted ; methods ; Models, Theoretical ; Plethysmography, Impedance ; methods ; Tomography ; methods

SQL language can achieve the rapid, accurate, efficient query in hospital of all the required information in the work, can supply a better service for the hospital management and decision. The methods is by using SQL language query hospital electronic medical records as the core of digital information. Compared to the artificial statistics, greatly improve the efficiency of statistical work. The conclusion is by using the SQL statement to exploring the relationship between the huge data in the database, to maximize the effectiveness of the data center, it is the necessary skill of in-formation manager.

OBJECTIVETo investigate the dynamic changes of type I collagen, and the activity of metalloproteinases-1 (MMP-1) and tissue inhibitor of metalloproteinases-1 (TIMP-1) after angioplasty.

METHODSThe restenotic model of iliac arteries of domestic microswine was established with hypercholesterol feed plus two angioplasties. Angioplastied vessels were harvested at the end of 1, 2, 3 and 6 months after the second angioplasty. Immunohistochemistry, transmission electronic microscopy and image quantitative analysis techniques were employed to study neointimal proliferation, the phenotype of vascular smooth muscle cells (VSMC) and the expression of type I collagen, MMP-1 and TIMP-1.

RESULTSThe peak of vascular neointimal proliferation was at 3 months after angioplasty. The expression of type I collagen gradually increased from 1 to 6 months after angioplasty. For MMP-1, expression was lower in the early stage after angioplasty but increase to normal levels of control vessels at 6 months after angioplasty. Expression of TIMP-1 rapidly increased in the early phase after angioplasty, reached peak at 3 months and maintained the high level till 6 months after angioplasty. Meanwhile, the VSMC was predominantly the synthetic phenotype at the early stage and was transformed to the contractive phenotype at the late stage after angioplasty. The ratio of TIMP-1 and MMP-1 was positively related to the area of the neointima and the expression of type I collagen respectively (P < 0.01).

CONCLUSIONType I collagen increased gradually after angioplasty, which might be determined by the ratio of TIMP-1/MMP-1 and also related to the phenotype of VSMC.

Angioplasty ; Animals ; Arterial Occlusive Diseases ; metabolism ; surgery ; Collagen Type I ; metabolism ; Iliac Artery ; surgery ; Matrix Metalloproteinase 1 ; metabolism ; Muscle, Smooth, Vascular ; cytology ; metabolism ; Swine, Miniature ; Tissue Inhibitor of Metalloproteinase-1 ; metabolism