Objective　Exploring how to check the proportional hazards assumption of the Cox model,and the solutions to non-proportional hazards between the covariates and the hazard function.Methods　With a example data set of Ⅲc stage ovarian serous cystadenocarcina,illustrating how to use graphical methods to check the proportional hazards assumption of the Cox model.Results　the predictor of post-surgery administering medicine times violated the proportional hazards assumption of the Cox model.Conclusion　when using the Cox model to analyze the predictors of survival time,checking whether the predictors violate the proportional hazards assumption of the Cox model or not should be paid attention to.

Objective　To improve the quality of papers on clinical diagnostic tests.　Methods　Articles of diagnostic tests published in the Chinese Journal of Oncology in the year 1990 were surveyed by cluster sampling method.　Results　In the 23 articles surveyed, immunoassays constituted the largest category, accounting for 39.1% (9/23), followed by biochemical markers and radiological tests, accounting for 17.4%（4/23） and 13.0%（3/23）,respectively. Of the 23 articles, 19 (82.6%) employed a well-defined “gold standard”,14 (60.9%) correctly calculated sensitivity and specificity. The interpretation of the test was stated to be “blind” in only 39.1%（9/32）. In only one paper were all the seven methodologic criteria adhered. Less than 60% of the articles followed more than four of the seven methodologic criteria, and in 2 articles only one of the criteria was met with.　Conclusion　Although due attention has been paid to the methodologic criteria of assessing diagnostic tests, the quality of the articles surveyed needs to be improved.

Objective　To improve the quality of papers on clinical diagnostic tests.　Methods　Articles of diagnostic tests published in the Chinese Journal of Oncology in the year 1990 were surveyed by cluster sampling method.　Results　In the 23 articles surveyed, immunoassays constituted the largest category, accounting for 39.1% (9/23), followed by biochemical markers and radiological tests, accounting for 17.4%（4/23） and 13.0%（3/23）,respectively. Of the 23 articles, 19 (82.6%) employed a well-defined “gold standard”,14 (60.9%) correctly calculated sensitivity and specificity. The interpretation of the test was stated to be “blind” in only 39.1%（9/32）. In only one paper were all the seven methodologic criteria adhered. Less than 60% of the articles followed more than four of the seven methodologic criteria, and in 2 articles only one of the criteria was met with.　Conclusion　Although due attention has been paid to the methodologic criteria of assessing diagnostic tests, the quality of the articles surveyed needs to be improved.

Objective To explore the influencing factors for postoperative adjuvant chemotherapy effects in elderly patients with breast cancer. Methods Five hundred and ninety female patients aged 65 years or older with invasive breast cancer were treated in our hospital, and the influencing factors for postoperative adjuvant chemotherapy effects were analyzed by chi-square test and logistic regression. Results Two hundred and thirty-one (39.2%) patients received postoperative adjuvant chemotherapy. The results showed that diabetes, age, patterns of operation and pathological characteristics of tumor had significant influences on postoperative adjuvant chemotherapy effects (χ2=4.49,88. 27,23.49 and 9.40, all P<0.05). Logistic regression analysis showed that age, tumor size, lymph node status(pN) and estrogen receptor (ER) status were related to postoperative adjuvant chemotherapy effects(χ2=68.857,15. 284,43. 540 and 7.009 ,all P<0.01). Forty-four patients (66.7%) with pN(+)/ER(-) received adjuvant chemotherapy. Conclusions Age, tumor size, lymph node status and ER status were independent predictive factors for postoperative adjuvant chemotherapy effects in elderly patients with breast cancer.

Objective To explore the correlation between personality traits and non suicidal self injury behavior in adolescent depression patients,in order to enhance understanding of the psychological health risks of depression patients.Methods This study was conducted at the Wuhan Mental Health Center from September 2021 to September 2023.A total of 138 patients with first-onset moderate to severe adolescent depression were included.The patients were divided into a group without self injury suicidal behavior(n=25),a non suicidal self injury(NSSI)group(n=78),and an attempted suicide(SA)group(n=35).Collect general information and data on neurotic personality scores,negative life events,emotional states,and social psychological factors using the Hamilton Anxiety Scale(HAMA),Hamilton Depression Scale(HAMD-24),and other psychological assessment tools.Using statistical methods for correlation and regression analysis to study the relationship between personality traits and non suicidal self injury behavior.Results There were significant differences in personality traits such as internal and external tendencies,psychoticism,neuroticism,and concealment among the non self injurious suicide behavior group,non suicidal self injurious behavior group,and suicide behavior group.The scores of the non suicidal self injurious behavior group and suicide behavior group were generally higher than those of the non self injurious self injurious behavior group(P<0.05);There were significant differences in self injury behavior scores among these three groups,especially in the suicide behavior group,which had the highest score,but there was no significant difference in life event scores;There were significant differences in scores for anxiety,depression,childhood trauma,stressful life events,and self-esteem among the three groups.Among them,the suicide behavior group had the highest scores for anxiety,depression,childhood trauma,and stressful life events,but the lowest score for self-esteem(P<0.05);Internal and external tendencies,psychoticism,neuroticism,and concealment are significantly correlated with non suicidal self injury behavior,and multiple regression analysis results show that these factors,as well as anxiety,depression,childhood trauma,stressful life events,and self-esteem,are inde-pendent influencing factors of non suicidal self injury behavior.The best predictive threshold for internal and exter-nal tendencies is-10.5%.Conclusion This study reveals the correlation between personality traits and non sui-cidal self injury behavior in adolescent depression patients.Understanding these relationships in clinical practice and intervention strategies can help better identify high-risk groups and provide personalized mental health support.

Objective:To investigate the effect of the number of positive preoperative serological tumor markers on the surgical approach and prognosis of patients with intrahepatic cholangiocarcinoma.Methods:This is a retrospective case-series study. Data from 548 patients with intrahepatic cholangiocarcinoma after radical resection from October 2010 to April 2019 were retrospectively collected in 10 hospitals of China. There were 277 males and 271 females with an age of (57.8±10.2)years(range:23 to 84 years). Four hundred and twenty-six patients(77.7%) had at least one positive preoperative serum tumor marker. The data collection included the results of 4 preoperative serological tumor markers,other preoperative indicators(5 prodromal symptoms, 6 medical history,8 preoperative serological indicators,5 preoperative imaging indicators,and 14 preoperative pathological examination indicators),baseline data (gender and age),surgical methods,and prognostic follow-up data. Four preoperative results of serologic tumor marker and surgical procedure were converted into categorical variables. The number of positive preoperative serum tumor markers was used as the treatment variable,the surgical method was used as the mediating variable,and the survival time was used as the outcome variable. Univariate and multivariate analysis were used to screen for other preoperative indicators which were independent factors that influenced the surgical procedure and the prognosis of patients as covariates to analyze the mediating effect.Results:Of the 548 patients included in the study, 176 patients (32.1%) underwent partial hepatectomy,151 patients(27.5%) underwent hemihepatectomy, and 221 patients(40.3%) underwent partial hepatectomy or hemihepatectomy combined with other treatments. The results of the univariate and multivariate analysis showed that the number of positive serum tumor markers,intrahepatic bile duct dilatation,portal vein invasion,pathological differentiation,pathological type,vascular invasion,T stage,N stage and maximum tumor diameter were independent factors influencing the surgical procedure(all P<0.05). Intrahepatic bile duct dilatation,pathological differentiation and T stage were independent prognostic factors for patients with intrahepatic cholangiocarcinoma(all P<0.05). Intrahepatic bile duct dilatation,differentiation and T stage were included as covariates in the mediation effect model. The results showed that the number of positive serum tumor markers before surgery had a negative predictive effect on the survival time of patients with intrahepatic cholangiocarcinoma ( β=-0.092, P=0.039),and had a positive predictive effect on the surgical method ( β=0.244, P<0.01). The number of positive serum tumor markers had a negative predictive effect on the survival time of patients with intrahepatic cholangiocarcinoma ( β=-0.151, P=0.002). Direct and indirect effects accounted for 71.3% and 28.7% of total effects,respectively. Conclusions:The higher the positive number of preoperative tumor markers,the worse the prognosis of patients with intrahepatic cholangiocarcinoma. The number of positive cells not only directly affects the prognosis of patients,but also indirectly affects the prognosis of patients by affecting the surgical method.

Objective:To investigate the effect of the number of positive preoperative serological tumor markers on the surgical approach and prognosis of patients with intrahepatic cholangiocarcinoma.Methods:This is a retrospective case-series study. Data from 548 patients with intrahepatic cholangiocarcinoma after radical resection from October 2010 to April 2019 were retrospectively collected in 10 hospitals of China. There were 277 males and 271 females with an age of (57.8±10.2)years(range:23 to 84 years). Four hundred and twenty-six patients(77.7%) had at least one positive preoperative serum tumor marker. The data collection included the results of 4 preoperative serological tumor markers,other preoperative indicators(5 prodromal symptoms, 6 medical history,8 preoperative serological indicators,5 preoperative imaging indicators,and 14 preoperative pathological examination indicators),baseline data (gender and age),surgical methods,and prognostic follow-up data. Four preoperative results of serologic tumor marker and surgical procedure were converted into categorical variables. The number of positive preoperative serum tumor markers was used as the treatment variable,the surgical method was used as the mediating variable,and the survival time was used as the outcome variable. Univariate and multivariate analysis were used to screen for other preoperative indicators which were independent factors that influenced the surgical procedure and the prognosis of patients as covariates to analyze the mediating effect.Results:Of the 548 patients included in the study, 176 patients (32.1%) underwent partial hepatectomy,151 patients(27.5%) underwent hemihepatectomy, and 221 patients(40.3%) underwent partial hepatectomy or hemihepatectomy combined with other treatments. The results of the univariate and multivariate analysis showed that the number of positive serum tumor markers,intrahepatic bile duct dilatation,portal vein invasion,pathological differentiation,pathological type,vascular invasion,T stage,N stage and maximum tumor diameter were independent factors influencing the surgical procedure(all P<0.05). Intrahepatic bile duct dilatation,pathological differentiation and T stage were independent prognostic factors for patients with intrahepatic cholangiocarcinoma(all P<0.05). Intrahepatic bile duct dilatation,differentiation and T stage were included as covariates in the mediation effect model. The results showed that the number of positive serum tumor markers before surgery had a negative predictive effect on the survival time of patients with intrahepatic cholangiocarcinoma ( β=-0.092, P=0.039),and had a positive predictive effect on the surgical method ( β=0.244, P<0.01). The number of positive serum tumor markers had a negative predictive effect on the survival time of patients with intrahepatic cholangiocarcinoma ( β=-0.151, P=0.002). Direct and indirect effects accounted for 71.3% and 28.7% of total effects,respectively. Conclusions:The higher the positive number of preoperative tumor markers,the worse the prognosis of patients with intrahepatic cholangiocarcinoma. The number of positive cells not only directly affects the prognosis of patients,but also indirectly affects the prognosis of patients by affecting the surgical method.

On January 22, 2020, China National Center for Bioinformation (CNCB) released the 2019 Novel Coronavirus Resource (2019nCoVR), an open-access information resource for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). 2019nCoVR features a comprehensive integra-tion of sequence and clinical information for all publicly available SARS-CoV-2 isolates, which are manually curated with value-added annotations and quality evaluated by an automated in-house pipeline. Of particular note, 2019nCoVR offers systematic analyses to generate a dynamic landscape of SARS-CoV-2 genomic variations at a global scale. It provides all identified variants and their detailed statistics for each virus isolate, and congregates the quality score, functional annotation,and population frequency for each variant. Spatiotemporal change for each variant can be visualized and historical viral haplotype network maps for the course of the outbreak are also generated based on all complete and high-quality genomes available. Moreover, 2019nCoVR provides a full collection of SARS-CoV-2 relevant literature on the coronavirus disease 2019 (COVID-19), including published papers from PubMed as well as preprints from services such as bioRxiv and medRxiv through Europe PMC. Furthermore, by linking with relevant databases in CNCB, 2019nCoVR offers data submission services for raw sequence reads and assembled genomes, and data sharing with NCBI. Collectively, SARS-CoV-2 is updated daily to collect the latest information on genome sequences, variants, hap-lotypes, and literature for a timely reflection, making 2019nCoVR a valuable resource for the global research community. 2019nCoVR is accessible at https://bigd.big.ac.cn/ncov/.