Objective To investigate the application of digital working system for the serving counter in major operation room. Methods We reestablished the streamline of the work at serving counter, we invented coordinate system of surgery arrangement platform-sign in-clothing changing machine-locker, established the rules of counter gate control system as well as protocol for clothing management in order to improve the overall quality and efficiency of the serving counter in the operation room. Results The digital working system of the serving counter could make the serving process running more programmed, normalized, standardized, institutionalized in the operation room. The satisfaction of first operation attendance rate, the dirty operating clothing collect rate, and operation management satisfaction from doctors were 92. 0%, 96. 0%, 97. 0%, which were higher than those of the control group (χ2 = 89. 90, 145. 72, 15. 34, respectively;P < 0. 01 ). Conclusions The application of digital working system can facilitate the service progress, normalize the management of the operation room, improve the efficiency of the operating room and upgrade the level of the management of infection in the operation room.