1.Limb-shaking transient ischemic attack responsive to nimodipine: A case report
Liang-Yong Li ; Chuan-Yong Yu ; Lin Huang ; Yu Wang
Neurology Asia 2012;17(4):353-356
Limb-shaking transient ischemic attack (TIA), a rare manifestation, is commonly caused by severe
stenosis or occlusion of an extracranial internal carotid artery. Such patients are usually treated with
surgical revascularization or anti-platelet therapy. We present a 56-year-old woman with 6 months’
episodic attacks starting with mouth skewed to the right and a sensation of ‘weakness’ involving
predominantly her left arm, and at times, also involved the left leg. This was immediately followed by
rhythmic jerky movements of the left arm and at times, also involved the left leg. Magnetic resonance
angiography revealed severe stenosis of M1 segment of the right middle cerebral artery. The patient’s
symptoms were signifi cantly improved by treatment with anti-platelet drugs and nimodipine.
3.Clinic study of plasma radiofrequency at low temperature in tonsillectomy.
Jie WANG ; Chuan DONG ; Chuan-yu LIANG ; Qihong FU ; Zhenhua JIANG ; Liling CHEN ; Li XIA
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(5):382-383
Adolescent
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Adult
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Child
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Child, Preschool
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Electrocoagulation
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methods
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Female
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Humans
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Hypothermia, Induced
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Male
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Middle Aged
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Prospective Studies
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Tonsillectomy
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methods
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Young Adult
4.Overexpression of Sox9 gene by the lentiviral vector in rabbit bone marrow mesenchymal stem cells for promoting the repair of cartilage defect.
Zhen WANG ; Da-chuan LIANG ; Jie-yu BAI ; Ning KANG ; Jun-yu FENG ; Zi-quan YANG
China Journal of Orthopaedics and Traumatology 2015;28(5):433-440
OBJECTIVETo study the overexpression of Sox9 gene on rabbit bone marrow mesenchymal stem cells for repairing articular cartilage injury in vivo.
METHODSRabbit bone marrow mesenchymal stem cells (BMSCs) were transduced with lentivirus vector containing Sox9 gene and then cartilage specific molecule was detected by RT-PCR in vitro. Total 48 knee joints of 24 mature New Zealand white rabbits were randomly divided into 3 groups according to different defect treatment. After animals anesthesia,a full-thickness cylindrical cartilage defect of 4 mm diameter and 3 mm deep was created in the patellar groove using a stainlesssteel punch. Meanwhile, the transfected cells were implanted to repair the rabbit model with full-thickness cartilage defects. Cartilage defects tissue was observed with light microscope, electron microscope, HE and immunohistochemistry staining to assess the repair of defects by the complex at 6 weeks or 12 weeks after the implantation.
RESULTSAt 3 days after the transfection, Sox9 gene expression was highest and Sox9 gene expression decreased with the increase of time. At 3 days after the transfection, the expression of collagen type II began and reached the peak at 14 days. It showed that the bone marrow mesenchymal stem cells went into chondrogenic differentiation after transfected by Sox9 gene. Histological observation showed that at 6 weeks after the operation, the defects in the experimental group was filled with hyaline like cartilage tissue, 12 weeks after operation,the defects of cartilage and subchondral bone had satisfactory healing. Both at 6 and 12 weeks postoperatively, the defects were filled with fibrous tissues in control groups. Meanwhile, immunohistochemical staining of sections with type II collagen antibodies showed the proteins in the regenerated tissue stained positive for type II collagen and stronger than the control groups. The histological scoring system indicated that the cartilage repair of experiment groups were better than the two control groups with statistical significances.
CONCLUSIONOverexpression of Sox9 gene on rabbit bone marrow mesenchymal stem cells (BMSCs) promote the repair of cartilage defect.
Animals ; Bone Marrow Cells ; metabolism ; Bone Marrow Transplantation ; Cartilage, Articular ; injuries ; metabolism ; Cell- and Tissue-Based Therapy ; Female ; Genetic Vectors ; genetics ; metabolism ; Humans ; Lentivirus ; genetics ; metabolism ; Male ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells ; metabolism ; Osteoarthritis ; genetics ; metabolism ; therapy ; Rabbits ; SOX9 Transcription Factor ; genetics ; metabolism ; Tissue Engineering
7.Identification and management of the maxillary sinus ostium in endoscopic sinus surgery.
Qin-xiu ZHANG ; Hui-ming AN ; Shi-xi LIU ; Chuan-yu LIANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(8):628-629
Adolescent
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Adult
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Aged
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Endoscopy
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Female
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Humans
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Male
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Maxillary Sinus
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surgery
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Middle Aged
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Otorhinolaryngologic Surgical Procedures
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methods
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Young Adult
8.Infection of different corneal surgeries on subbasal nerves
Ying, YANG ; Jing, ZUO ; Chuan-Wei, ZHANG ; Kai, LI ; Yu-Liang, WANG
International Eye Science 2015;(8):1429-1431
AIM:To observe the recovery of corneal subbasal nerves after laser - assisted subepithelial keratomileusis (LASEK), laser in situ keratomileusis ( LASIK), femto-second lenticule extraction ( FLEx ) and small incision lenticule extraction ( SMILE) .
METHODS: Confocal microscopy was used to observe subbasal nerves 1mo after surgery in 12 eyes of 12 LASEK patients, 12 eyes of 12 LASIK patients, 12 eyes of 12 FLEx patients and 12 eyes of 12 SMILE patients as well as some other follow-up times.
RESULTS: Subbasal nerves 1mo after SMILE were almost complete and regular, showing no significant differences from those 2wk after surgery or even unoperated eyes. The nerves cut off at the incision were well involuted 1mo after surgery. Subbasal nerves were damaged in different degrees and got repaired to form communicating branches with time lapse after LASEK, LASIK and FLEx.
CONCLUSION: SMILE exerted small infections on subbasal nerves. It may be superior to other corneal refractive surgeries in terms of postoperative nerve recovery.
9.Metal stents in the treatment of neoplasm causing bronchial obstruction.
Guo-liang SHAO ; Chuan-ding YU ; Yu-tang CHEN ; Yan-ping YU ; Qi-rong XIA ; Wei-sheng LIAN
Chinese Journal of Oncology 2005;27(7):444-445
Aged
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Bronchoscopy
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Esophageal Neoplasms
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complications
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Female
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Humans
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Male
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Middle Aged
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Stents
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Thyroid Neoplasms
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complications
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Tracheal Stenosis
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etiology
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therapy
10.Laparoscopic high ligation of spermatic vein in the treatment of critical varicocele
Shao-Lin ZHANG ; Bo-Chuan CHEN ; Yong-Hua CHEN ; Wei-Dong LIANG ; Liang BAI ; Yu-Xiang ZHONG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(12):-
Objective To probe into the clinical curative effect of laparoscopic high ligation of spermatic vein in the treatment of critical varicocele.Methods The clinical data of the 26 eases of laparoscopic high ligation of apermatic vein in the treatment of critical varicocde were reviewed and analyzed in the last two years in this hospi- tal.Results All the 26 cases had been conducted smoothly with the operation time 25~50min,an average of(28?3)min.After 3~24 months being followed-up,all the symptoms and signs disappeared with no relapse and testicle a- trophy.Eight wives of the patients who had been operated on got pregnant.Conclusion With soon recovery and small wound,it was safe to adopt laparoscopic high ligation of spermatic vein in the treatment of critical varicocele, especially for critical two-sided varicocde.