OBJECTIVETo evaluate the clinical effectiveness of transurethral seminal vesiculoscopy (TUSV) combined with finasteride in the treatment of recurrent hemospermia.

METHODSThis study included 32 patients with recurrent hematospermia, with the disease course of 3 months to 4 years. After administration of finasteride at 5 mg/d for 2 weeks, the patients underwent TUSV for both exploration of the causes and treatment, followed by medication with finasteride at the same dose for another 2 weeks. Postoperative follow-up was conducted for observation of the outcomes and complications.

RESULTSTUSV was successfully accomplished in all the 32 cases, which revealed 16 cases of seminal vesiculitis, 10 seminal calculi, 1 seminal vesicle cyst, 2 seminal vesicle polyps, and 3 seminal vesicle abscess. The operative time was 20 to 51 (31.0 +/- 5.2) minutes. Postoperative complications included 1 case of acute epididymitis and 3 cases of breast discomfort within the first 4 weeks. No incontinence, urethral stricture, rectal injury, retrograde ejaculation, and sexual dysfunction occurred postoperatively. All the patients but 1 were followed up for 6 months to 2 years. Twenty-nine of the cases were cured, and 2 experienced recurrence.

CONCLUSIONTransurethral seminal vesiculoscopy combined with finasteride is safe and effective for the treatment of recurrent hemospermia.

Adult ; Endoscopy ; methods ; Finasteride ; therapeutic use ; Follow-Up Studies ; Hemospermia ; therapy ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Treatment Outcome

To study the contribution of T cell subsets in the pathogenesis of Murine hepatitis virus Type3 (MHV-3) induced chronic viral hepatitis in C3H/Hej mice, ninety C3H/Hej mice were chosen to individually receive 10 plaque forming units (PFU) of MHV-3 intraperitoneally. The changes of virus titer and pathology in liver tissue were examined by standard plaque assay and by the hematoxylin/eosin (HE) staining method from 2 days post MHV-3 infection. The ratios of T cell subsets including CD3+CD4+CD8-, CD3+CD4-CD8+, CD3+CD4-CD8-, CD3+CD4+CD25+, CD3+CD4+CD25- and CD3+CD4-CD25+ T lymphocyte of total T lymphocytes in blood, spleen and liver were examined at 0, 2, 4, 6,8, 10, 12, 15, 20, 25, 30, 40 days post MHV-3 infection by flow cytosorting. We observed that the virus titer raised and showed persistent virus duplications and inflammatory changes in the livers of C3H/Hej mice from 2 days post MHV-3 infection. The double negative T cell (DN Treg cell) and CD4+CD25+ T cell ratios increased significantly from 2 days post MHV-3 infection in C3H/Hej mice, and CD3+CD4+CD8-, CD3+CD4-CD8+, CD3+CD4+CD25- and CD3+CD4-CD25+ T cell ratios decreased accordingly. In conclusion, the changes of virus titer and pathology in the livers of C3H/Hej mice post MHV-3 suggest their contribution to viral persistence. Further characterizations of DN Treg cells are that infection indicates that MHV-3 could induce the chronic inflammation in livers of C3H/Hej mice.The increase of the DN Treg cell and CD4+CD25+ T cell ratios in C3H/Hej mice post MHV-3 infection suggests that DN Treg cells and CD4+CD25+ T cells may both have important suppressive immunomodulation functions in the development of chronic viral hepatitis and have important roles in the virus persistent infection. Further characterizations of DNT cell and CD4+CD25+ T cell are under investigation.

ObjectiveTo explore the mechanism of extracorporeal shock wave (ESW) in treating osteogenic disorders and the ideal energy level. MethodsAfter success in marrow aspiration from patients' iliac crest, hMSCs were isolated by Percoll density gradient centrifugation and cultured in Dulbecco's modified Eagle's medium in a 5% CO2 and 37 ℃ incubator. Optimal ESW dose was determined by MTT of kinase-marked cytobiology. After hMSCs were exposed to ESW, their morphocytologic change, rate of adherence and doubling time were observed with IPCM. Enzyme cytochemistry reaction for the activity of alkaline phosphatase was also examined. ResultsESW of 5 kV and 100 times could increase cells' viability and proliferation (P<0.01), but higher than 7 kV would inhibit them. Rate of adherence of hMSCs in exposure group of passage 5 reached to 61.54%, which was significantly different from control group(P<0.05). Compared with control group, the MSCs' doubling time was short for 1.72 d (P<0.05). The curve of normal alkaline phosphatase activity of hMSCs was like type S, but ESW shortened its latent period, and promoted its peak time, which was significantly different from control group.ConclusionESW of 5 kV and 100 times can optimally promote the proliferation and activity of osteogen of hMSCs in vitro.

Ethnicity ; Forensic Medicine ; Humans ; Polymorphism, Genetic

Objective:To investigate the therapeutic effect of Weichangning granule combined with compound Lactobacillus acidophilus tablet on diarrhea type irritable bowel syndrome and its effect on inflammatory factors, gastrointestinal hormones and intestinal mucosal barrier.Methods:From January 2017 to December 2019, 80 patients with diarrhea type irritable bowel syndrome admitted in the First People's Hospital of Fuyang District were selected, and they were divided into observation group and control group according to the random digital table method, with 40 cases in each group.The control group was treated with relapsed Lactobacillus acidophilus tablets, and the observation group was treated with Weichangning granules on the basis of the control group.The course of treatment in both two groups was 2 weeks.The changes of inflammatory factors, gastrointestinal hormones and intestinal mucosal barrier were compared before and after treatment.Results:The total effective rate of the observation group[92.50%(37/40)] was higher than that of the control group[70.00%(28/40)], the difference was significant (χ 2=6.646, P<0.05). The levels of TNF-α[(36.15±10.12)ng/L], IL-1β[(0.43±0.08)μg/L] and IL-6[(12.18±3.06)ng/L] in the observation group were lower than those in the control group[(54.36±15.73)ng/L, (0.79±0.12)μg/L and (18.53±2.19)ng/L]( t=6.157, 15.787, 10.673, all P<0.05). The serum levels of SS[(28.91±11.02)μg/L] and VIP[(185.46±17.34)ng/L] in the observation group were lower than those in the control group[(45.63±12.54)μg/L and (235.12±18.29)ng/L]( t=6.334, 12.462, all P<0.05). The levels of DAO[(1.05±0.19)U/L], endotoxin[(0.62±0.19)U/L] and D-lactate[(38.97±6.39)mg/L] in the observation group were lower than those in the control group[(1.53±0.28)U/L, (1.16±0.23)U/L and (49.81±4.25)mg/L]( t=8.972, 11.448, 8.934, all P<0.05). Conclusion:Weichangning granule combined with compound Lactobacillus acidophilus tablet has obviously curative effect on diarrhea type irritable bowel syndrome, which can reduce inflammatory reaction, improve gastrointestinal hormone and intestinal mucosal barrier function.

Objective:To observe the effect of electroacupuncture (EA) on the expressions of acetylcholine (ACh) and mucin 5AC (MUC5AC) in the lungs of rats with chronic obstructive pulmonary disease (COPD),and explore the mechanism of EA in treating COPD.Methods:Thirty Sprague-Dawley (SD) rats were randomly divided into a control group,a COPD group,and an EA group,with 10 rats in each group.The control group was a group of normal rats.The COPD rat model was induced by cigarette smoke combined with lipopolysaccharide (LPS).The COPD rats were treated with EA at bilateral Feishu (BL 13) and Zusanli (ST 36) in the EA group,30 min each time,once a day,successively for 14 d.The lung function was tested.The contents of ACh and MUC5AC in lungs and bronchoalveolar lavage fluid (BALF) were detected by enzyme-linked immunosorbent assay (ELISA).Pearson method was used to analyze the correlation between pulmonary function and the content of MUC5AC in lungs.The mRNA and protein expressions of MUC5AC in lung tissues were detected by real-time polymerase chain reaction (RT-PCR) and Western blot (WB),respectively.The immune response of MUC5AC was observed by immunohistochemistry.Results:Eight rats were left in each group,and the other two died.Compared with the control group,the total airway resistance (Raw) increased significantly and dynamic compliance (Cdyn) decreased significantly in the COPD group (P＜0.01);compared with the COPD group,the Raw level declined significantly and Cdyn increased significantly in the EA group (P＜0.01).The contents of ACh and MUC5AC in the lungs and BALF were remarkably higher in the COPD group compared with those in the control group (P＜0.01,P＜0.001);compared with the COPD group,the contents of ACh and MUC5AC were significantly lower in the EA group (P＜0.05,P＜0.001).There was a negative correlation between MUC5AC content and lung function (P＜0.001).The mRNA and protein expressions of MUC5AC in the lungs were significantly higher in the COPD group than in the control group (P＜0.001);compared with the COPD group,the expressions were significantly lower in the EA group (P＜0.01).Compared with the control group,the immune response of MUC5AC in the airway epithelium significantly increased in the COPD group (P＜0.001);the immune response of MUC5AC was significantly lower in the EA group compared with that in the COPD group (P＜0.001).Conclusion:EA treatment can improve the lung function of COPD rats,which may be related to its effect in the down-regulation of ACh and MUC5AC contents in the lungs as well as the inhibition of mucus hypersecretion.

OBJECTIVETo compare the three dimensional morphology and surface roughness changes of enamel eroded for different etching time.

METHODSFifteen freshly extracted sound human pre-molars for orthodontic purpose were collected. The buccal surface of teeth were prepared into smooth enamel slices, and then were randomly divided into 5 groups based on their etching time 0 s (control group), 5 s, 10 s, 20 s, 30 s, respectively by 37% phosphoric acid. The three dimensional morphology was observed under atomic force microscope (AFM). The profile was analyzed, and the value of Ra, Rq, Rz and the surface area and volume were measured.

RESULTSThe AFM photograph showed that with the etching time from 0 s to 20 s the enamel surface demineralised gradually, the top structure of enamel rod and the fish scaled structure became obvious. But the morphology only changed a bit after 20 s. The surrounding inter-rod enamel eroded first, the depth increased to 2.8 µm at 20 s but decreased to 1.8 µm at 30 s. The value of Ra increased from (19.69 ± 3.42) nm to (359.51 ± 75.79) nm, and Rq from (22.02 ± 5.57) nm to (431.02 ± 83.09) nm, Rz from (0.24 ± 0.08) µm to (2.38 ± 0.26) µm. Except for groups 20 s and 30 s, the difference among other groups was statistically significant (P < 0.05). The surface area expanded from (406.77 ± 3.88) µm(2) to (546.69 ± 84.02) µm(2), and surface volume from (65.73 ± 14.46) µm(3) to (474.63 ± 52.50) µm(3).

CONCLUSIONSThe depth, surface roughness, surface area and volume caused by erosion increased with etching time. The three dimensional morphology greatly changed by acid-etching process.

Acid Etching, Dental ; methods ; Analysis of Variance ; Dental Enamel ; drug effects ; ultrastructure ; Humans ; Microscopy, Atomic Force ; Phosphoric Acids ; pharmacology ; Random Allocation ; Surface Properties ; drug effects ; Time Factors

Gene chip technology was employed to study gene expression in Tamarix androssowii under NaHCO3 stress. cDNAs from T. androssowii treated with NaHCO3 solution and that from control group were labeled with fluorescent dye CyS and Cy3 respectively. The two fluorescent cDNA probes were mixed and hybridized to gene chips containing T. androssowii genes, and the chips were scanned using biochip scanning system. Differential expression of genes was analyzed through calculation of the ratio of Cy5 to Cy3 signal intensities. Total of 89 genes differentially expressed were identified, among them, 27 showed down regulated expression and 62 showed up regulated expression. Blastx analysis showed that the function of the differentially expressed genes could be grouped into some categorizations such as photosynthesis, reactive oxygen species eliminated, regulation of osmotic potential, regulation of gene expression and signal transduction, metabolism, development, ribosomal protein, protein breakdown and recycling, transporter, water channel proteins and so on. Based on this research, some function-unknown or novel unreported genes that respond to salt stress were also identified, and these genes may have important functions in salt resistance of T. androssowii. Some important pathways of salt resistance in T. androssowii are revealed, and the gene expression profiling of T. androssowii under salt stress and without stress is obtained in this study.
DNA, Complementary ; genetics ; Gene Expression Profiling ; methods ; Genes, Plant ; genetics ; Oligonucleotide Array Sequence Analysis ; Sodium Bicarbonate ; pharmacology ; Stress, Physiological ; Tamaricaceae ; drug effects ; genetics

Purpose To investigate the pathological features and prognostic value of tertiary lymphoid structure (TLS) formation in gastric cancer (GC) . Methods HE staining slides were reviewed to evaluate the TLS in 163 specimens from patients with GC in the Third Affiliated Hospital of Soochow University from 2006 to 2008. The validation cohort contained 63 randomly selected cases and immunohistochemical staining of MECA-79 was used to verify the accuracy of pathological assessment of TLS. Results TLS score and MECA-79 immunohistochemical staining showed significant correlation (P = 0. 002) and agreement (P = 0. 024) . The TLS was not significantly correlated to clinical pathological parameters. The patients with high level of TLS had better prognosis (P = 0. 025) with the mean survival time of 48. 54 months. In multivariate Cox regression analysis, TLS was an independent prognostic factor (P = 0. 031) . Conclusion The pathological evaluation of TLS is accurate. The formation of TLS is an important positive prognostic factor for GC patients.

During the past 70 years since the founding of New China, Chinese public health especially the prevention and control of infectious diseases have made remarkable achievements, which benefited from the vaccination. This study is to summarize the progress of immunization and vaccines, the achievements and contributions of vaccines including polio vaccine, hepatitis B vaccine, diphtheria, tetanus and acellular pertussis combined vaccine, measles vaccine and hepatitis A vaccine to the prevention and control of infectious diseases in China in the past 70 years and to review the research and development of innovative vaccines in China in recent years, which may provide clues for the development of the expanded programe on immunization in China in the future.