1.Effect of nano-SiO2 exposure on spatial learning and memory and LTP of hippocampal dentate gyrus in rats.
Huan-rui SUN ; Na ZHANG ; Xue-wei CHEN ; Gai-hong AN ; Chuan-xiang XU ; Qiang MA
Chinese Journal of Applied Physiology 2016;32(1):78-81
OBJECTIVETo study the effect of nano-SiO2 on spatial learning and memory.
METHODSTwenty-four male rats were randomly divided into 3 groups: control group (C group), low dose group (L group) and high dose group (H group). The rats were intragastrically administrated with nanometer particles at 25 and 100 mg/kg body weight every day for 4 weeks. After exposure, the ability of learning and memory of rats was tested by Morris water maze, and electrophysiological brain stereotactic method was used to test long-tear potentiation (LTP) in dentate gyrus (DG) of the rats.
RESULTSThe increase rate of body weight in H group was reduced significantly compared with C group ( P < 0.05). In the space exploration experiment of Morris water maze test, the escape latency of H group was longer than that of C group (P < 0.05). The rats of H group spent less time in finding the target quadrant (P < 0.05) . The rate of LP induction of H group was significantly lower than that of C group (P < 0.05). After high fre quency stimulation (HFS), The changes of amplitude of population spike (PS) of L group and H group were lower than those of C group significantly (P < 0.05, P < 0.01).
CONCLUSIONNano-SiO₂may result in impairment of spatial learning and memory ability by reducing the rate of LTP induction and the increase of PS in hippocampus.
Animals ; Dentate Gyrus ; drug effects ; Long-Term Potentiation ; drug effects ; Male ; Maze Learning ; drug effects ; Memory ; drug effects ; Nanoparticles ; adverse effects ; Rats ; Silicon Dioxide ; adverse effects ; Spatial Learning ; drug effects
2.Value of computed tomography and magnetic resonance imaging in diagnosis and differential diagnosis of small hepatocellular carcinoma.
Yan MA ; Xue-lin ZHANG ; Xin-yu LI ; Lin ZHANG ; Huan-huan SU ; Chuan-yin ZHAN
Journal of Southern Medical University 2008;28(12):2235-2238
OBJECTIVETo analyze the computed tomography (CT) and magnetic resonance imaging (MRI) findings of small hepatocellular carcinoma to improve the accuracy in the diagnosis.
METHODSThis retrospective analysis involved 41 patients with small hepatocellular carcinoma cases confirmed by pathological examination of the biopsy samples or follow-up. These patients were assessed for CT and MRI findings including lesion size, density or signal intensity, enhancement patterns, and presence of tumor capsules.
RESULTSOn unenhanced CT images, small hepatocellular carcinomas were displayed mainly as low-density masses, and the majority of tumors presented with low signal intensity on T1-weighted unenhanced MR images with increased signal intensity on T2-weighted images in comparison with the surrounding liver parenchyma. Most of tumors showed intense enhancement during the arterial phase (CT in 15 cases and MRI in 13 cases), but some appeared isointense to the liver parenchyma (CT in 4 cases and MRI in 4 cases). In portal and delayed phases, the tumors typically had lower signal intensity than that of the surrounding liver tissues (CT in 25 cases and MRI in 12 cases) with enhancement of the tumor capsules (13 cases).
CONCLUSIONDynamic enhanced scanning can be more informative of the pathology and blood supply of small hepatocellular carcinoma. Early and late arterial phase imaging may help in detecting the small lesions and in making differential diagnosis.
Adult ; Aged ; Carcinoma, Hepatocellular ; diagnosis ; diagnostic imaging ; Diagnosis, Differential ; Female ; Humans ; Image Enhancement ; Liver Neoplasms ; diagnosis ; diagnostic imaging ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Retrospective Studies ; Tomography, X-Ray Computed
3.Immune Responses and Histopathological Changes in Rabbits Immunized with Inactivated SARS Coronavirus
Chuan-hai, ZHANG ; Xin-jian, LIU ; Yi-fei, WANG ; Jia-hai, LU ; Huan-ying, ZHENG ; Sheng, XIONG ; Mei-ying, ZHANG ; Qiu-ying, LIU
Virologica Sinica 2007;22(5):360-365
To evaluate the immunogenicity of inactivated SARS coronavirus (SARS-CoV), three groups of rabbits were immunized three times at 2-week intervals with inactivated vaccine + adjuvant, adjuvant,and normal saline respectively. Eight batchs of serum were sampled from the auricular vein at day 7 to day 51, and specific IgG antibody titers and neutralizing antibody titers were detected by indirect ELISA and micro-cytopathic effect neutralizing test. Antibody specificity was identified by proteinchip assay.Histopathological changes were detected by H&E staining. The results showed that, rabbits in the experimental group immunized with inactivated SARS-CoV all generated specific IgG antibodies with neutralizing activity, which suggested the inactivated SARS-CoV could preserve its antigenicity well and elicit an effective humoral immune responses. The peak titer value of specific IgG antibody and neutralizing antibody reached 1:40960 and 1:2560 respectively. In the experimental group, no obvious histopathological changes was detected in the H&E stained slides of heart, spleen, kidney and testis samples, but the livers had slight histopathological changes, and the lungs presented remarkable histopathological changes. These findings are of importance for SARS-CoV inactivated vaccine development.
4.Prevalence and risk factors of erectile dysfunction in three cities of China: a community-based study.
Quan BAI ; Qing-Quan XU ; Hui JIANG ; Wei-Li ZHANG ; Xing-Huan WANG ; Ji-Chuan ZHU
Asian Journal of Andrology 2004;6(4):343-348
AIMTo determine the age-adjusted prevalence of erectile dysfunction (ED) in 3 big cities of China and to explore its potential sociodemographic, medical and lifestyle correlates.
METHODSA cross-sectional, population-based survey was conducted in three cities of China. Structured questionnaires were administered to 2 226 men, aged 20 - 86 years, by trained interviewers.
RESULTSThe age-adjusted prevalence of ED was 28.34 % (mild 15.99 %, moderate 7.14 %, severe 5.21 %). In the men above 40, the prevalence was 40.2 %. Age was positively correlated with ED (P<0.01). Education was negatively correlated with ED (P<0.01). Spouse companionship, living condition were positively correlated with ED (P<0.01). Histories of cardiovascular disease, diabetes, and hyperlipidemia were positively correlated with ED (P<0.01). Cigarette smoking was not correlated with ED (P>0.05), while the cigarette consumption and duration were positively correlated with ED (P<0.01). Alcohol drinking is negatively correlated with ED (P<0.01). The duration of drinking was positively correlated with ED (P<0.01). Weekly alcohol consumption was not correlated with ED (P>0.05).
CONCLUSIONThe prevalence of ED increased with age. Cardiovascular disease, diabetes and hyperlipidemia were positively correlated with the increased prevalence. Sociodemographic and lifestyle factors, such as education, spouse companionship, living condition, cigarette and alcohol consumption or duration also have association with the prevalence of ED.
Adult ; Age Factors ; Aged ; Alcohol Drinking ; adverse effects ; Cardiovascular Diseases ; complications ; epidemiology ; China ; Cross-Sectional Studies ; Diabetes Mellitus ; epidemiology ; Education ; Erectile Dysfunction ; epidemiology ; Humans ; Hyperlipidemias ; complications ; epidemiology ; Life Style ; Male ; Marriage ; Middle Aged ; Population ; Risk Factors ; Smoking ; physiopathology ; Socioeconomic Factors ; Surveys and Questionnaires
5.A 3-year-follow-up study on the prognosis of ‘two-route chemotherapy’ on liver cancer patients with portal vein tumor thrombus
Huan-Hu ZHANG ; Sheng-Bo SUN ; Chuan-Ji HAN ; Shao-Wei SUN
Chinese Journal of Epidemiology 2012;33(11):1181-1183
Objective To study the achievements and safety of Transcatheter arterial chemoembolization (TACE) associated Portal Vein Chemo-therapy (PVC) per-drug delivery system (DDS) program in preventing the recurrence of hepatic cell cancer (HCC) and Portal Vein Tumor Thrombus (PVTT).Methods 97 cases with HCC and PVTT were treated from Januay 2009 to January 2011.Patients with tumor or tumor thrombus were resected on all the cases and randomly divided into 3 groups.TACE,PVC per-DDS TACE and PVC per-DDS were given to group A,group B,and group C,respectively.Patients in the 3 groups were followed and compared on the Disease Free Survivals (DFS) and the accumulative survival rates,at 6 months,1 year and 2 years after the operation.Results After the surgery was completed in June,the 1-year,2-year,3-year survival rates and cummulative survival rate in group C was higher than in group A or group.Significant differences did no appeare in June but did show in 1 year after the surgery (P>0.05) as well as in both 2 and 3 years,after the surgery (P<0.01).Conclusion Patients with HCC and PVTT,the TACE chemotherapy in association with PVC per-DDS could increase both the DFSs and accumulative survival rates,when compared to the either single TACE or PVC per-DDS,after the tumor or tumor thrombus were resected.
6.Establishment and analysis of chronic periodontitis and atherosclerosis model in Wistar rat
Liang-Jun ZHONG ; Jun XU ; Yuan-Ming ZHANG ; Jia NI ; Xiao-Huan ZHOU ; Fang-Chuan SHI
Chinese Journal of Stomatology 2009;44(8):464-468
lerosis. MMP-3 may be an independent risk factor of atherosclerosis exclude TC and LDL
8.The relationship between emotional,physical abuse and internet addiction disorder among middle school students
Zhi-Hua ZHANG ; Jia-Hu HAO ; Lin-Sheng YANG ; Xiu-Jun ZHANG ; Ye-Huan SUN ; Chuan-Lai HU ; Dong-Qing YE ; Fang-Biao TAO
Chinese Journal of Epidemiology 2009;30(2):115-118
Objective To study the relationship between emotional,physical abuse and internet addiction disorder(IAD)among middle school students.in order to lay foundation for the development of prevention and control programs on IAD.Methods Students selected from 76 classes in Grade One and Grade Two,filled out the anonymous questionnaire,which including demographic characteristics of students,Young's Intemet Addiction Scale,Parent-Child Conflict Tactics Scales.etc.Non-conditional multivariate logistic regression model was employed to explore the relationship between child abuse and IAD.Independent variable was child abUSe while dependent variable was IAD.Covariant variables would include demographic characteristics,as sex,age,style of household,et al.Results Among 3798 students,the overail prevalence of emotional abuse was 81.1%.with 55.1% of them reported having experienced physical abuse.Among 3507 intemet users,5.2%was diagnosed as IAD.Results from the nonconditional multivariate Iogistic regression model showed that odds ratio of severe physical abuse and moderate physical abuse were 3.02(1.63-5.58)and 4.00(2.01-7.93).Conclusion Moderate physical abuse and severe physical abuse were possible risk factors of IAD.
9. Comparison of clinical effect between autogenous semitendinosus and allogenic tendons for anatomical reconstruction of anterior talofibular ligament and calcaneofibular ligament under arthroscopy
Yue XU ; Weiping LI ; Chuan JIANG ; Zhengzheng ZHANG ; Yunfeng ZHOU ; Huan LUO ; Haozhi ZHANG ; Bin SONG
Chinese Journal of Orthopaedics 2019;39(11):667-674
Objective:
To compare the clinical effects of autologous semitendinosus tendon and allogenic tendon arthroscopic anatomical reconstruction of anterior talofibular ligament (ATFL) combined with calcaneofibular ligament (CFL) in the treatment of chronic lateral ankle instability.
Methods:
A retrospective analysis was made of 55 patients with chronic lateral ankle instability who underwent arthroscopic reconstruction of ATFL combined with CFL from January 2012 to June 2017. A total of 28 cases were treated with autologous semitendinosus tendon (autologous group), including 19 males and 9 females, with an average age of 28.5±8.03 years (range, 16-46 years). A total of 27 cases were treated with allogenic tendon (allogenic group), including 17 males and 10 females, with an average age of 27.48±7.89 years (range, 16-46 years). ATFL/CFL was reconstructed by the same method in both groups. The reconstruction methods were the same between the groups. The talus and calcaneus were fixed with absorbable compression nails.
Results:
The operation duration in the autologous group was 94.07±7.83 min, which was longer than that in the allogeneic group 63.56±7.96 min (
10.Preparation antibodies against recombinant bovine IFN-gamma and development of sandwich ELISA for bovine IFN-gamma detection.
Chuan LI ; Ya-Di TAN ; Ying-Yut CHEN ; Qiao-Yun HU ; Zhang Gui-Rong YAN ; Bo QIN ; Yan-Jie CHAO ; Huan-Chun CHEN ; Ai-Zhen GUO
Chinese Journal of Biotechnology 2007;23(1):40-45
This study was aimed to establish ELISA for recombinant bovine IFN-gamma (BovIFN-gamma) detection and provide a new method for diagnosis of pathogenic infection. The total RNA was isolated from peripheral blood leucocytes cultured with PHA mitogen stimulation. Then bovine IFN-gamma (BovIFN-gamma) gene cDNA was amplified by RT-PCR and cloned into pET28a to obtain the expression plasmid designated as pETBovIFN-gamma. The pETBovlFN-gamma was further transformed into competent E. coli BL21 cells and a 18kD His-tagged protein as expected was expressed after IPTG induction. By using purified recombinant BovIFN-gamma as antigen and lymphocyte-hybridoma technique, four hybridoma cell lines which stably secreted monoclonal antibodies against rBovIFN-gamma were generated, designated as A7, A10, G6, and G10. The immunoglobin subset was identified as IgG1 . Western-blotting analysis and ELISA demonstrated that the monoclonal antibodies secreted by all the four hybridoma cell lines could react specifically to the recombinant BovIFN-gamma, but not irrelative proteins such as Ag85B, ESAT-6-CFP-10 and GM-CSF, suggesting that the four hybridoma cell lines were rBovIFN-gamma specific monoclonal antibodies. A sandwich ELISA was established by using A10 secreted monoclonal antibody and rabbit polyclonal antibodies against BovIFN-gamma, HRP labeled goat anti-rabbit IgG. The results indicated that the sensitivity was 2ng/mL. This sandwich ELISA to detect BovIFN-gamma paved the way to develop a sensitive method for specific infection detection such as bovine tuberculosis diagnosis.
Animals
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Animals, Newborn
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Antibodies, Monoclonal
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biosynthesis
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immunology
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Antibody Specificity
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immunology
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Blotting, Western
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Cattle
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Cells, Cultured
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DNA, Complementary
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genetics
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Enzyme-Linked Immunosorbent Assay
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methods
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Female
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Hybridomas
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Interferon-gamma
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genetics
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immunology
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metabolism
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Mice
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Mice, Inbred BALB C
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Plasmids
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genetics
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Rabbits
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Recombinant Proteins
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immunology
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metabolism
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Reverse Transcriptase Polymerase Chain Reaction