1.Correlation study between ~(18)F-FDG uptake and hypoxia inducible factor-1α level,microvessel density in human gliomas
Li, CAI ; Chuan, ZHANG ; Yan-sheng, LI ; Da-cheng, LI ; Xi-ling, XING ; Shuo, GAO
Chinese Journal of Nuclear Medicine 2010;30(1):10-14
Objective To investigate the correlation between ~(18)F-fluorodeoxyglucose (FDG) uptake and hypoxia inducible factor1α (HIF-1α) level,microvessel density (MVD) in human gliomas.Methods ~(18)F-FDG PET scan was performed preoperatively in 41 patients with gliomas (including 23 highgrade and 18 low-grade tumors).The ratios of maximum standardized uptake value(SUV_(max))between tumor (T)and contralateral white matter (WM) were calculated (T/WM).Immunohistochemical stain methods were used to evaluate the level of HIF-1α and measure the MVD in tumors.Correlation analysis between SUV_(max) of T/WM and HIF-1α level,MVD wag performed.The t-test,one-way ANOVA test,Spearman rank correlation and Wilcoxon signed-rank test were calculated using SPSS 11.5 software.Results (1)The SUV_(max) of T/WM,HIF-1α level and MVD in high-grade and low-grade tumors groups were 3.39±1.43,95.7% and 44.13±16.1 vs 1.46±0.55.55.6% and 18.83±7.07,respectively.The difierences of SUV_(max) of T/WM,HIF-1α level and MVD between two groups were statistically significant (t=-5.921,z=-3.938,t=-6.745,all P<0.05).(2)Among 41 gliomas,the strong positive expression of HIF-1α was observed in 8,mederate in 9,weak in 15 and negative expression was found in 9,SUV_(max) of T/WM and MVD increased with increasing HIF-1α level.The differences of SUV_(max) of T/WM and MVD among 4 different groups were statistically significant (F=7.41,P<0.05).(3) The MVD of all gliomas was ranged from 9.76 to 94.52,which correlated with SUV_(max) of T/WM(r=0.759,P<0.05).Conclusions The SUV_(max) of T/WM correlates with HIF-1α level and MVD in gliomas.Therefore,~(18)F-FDG PET provides preoperatively a noninvasive assessment of hypoxia or angiogenesis in human glionma.
2.Effect of globular adiponectin on the function of NIT-I cells under high glucose medium
Jia ZHOU ; Yan LI ; Chuan YANG ; Mingtong XU ; Fangping LI ; Li YAN ; Hua CHENG ; Zuzhi FU
Chinese Journal of Endocrinology and Metabolism 2009;25(3):316-317
The effect of globular adiponectiin (gAd)on the function of NIT-1 cells under high glucose medium was investigated. The results showed that gad could completely block the increase of NADPH oxidase components p47phox expression and recover mRNA expression of pancreatic duodenal homeobox-I ,paired box gene 6,glucose transpoter 2,and glucokinase except neurogenic differentiation factor 1 (P<0.05 or P<0.01). Whereas,impaired insulin secretion and mRNA expression at high glucose concentration were not significantly improved by gAd.
3.Effect of the anterior aspect of sacral nerve root tunnel on iliosacral screw placement on the standard lateral image of sacrum.
Hong-Min CAI ; Chuan-De CHENG ; Xue-Jian WU ; Wu-Chao WANG ; Jin-Cheng TANG ; Wei-Fang DUAN ; Chuan ZHANG ; Hong-Wei LI ; Wu-Yin LI
China Journal of Orthopaedics and Traumatology 2014;27(4):326-330
OBJECTIVETo introduce the location and course of S1, S2 sacral nerve root tunnel and to clarify the significance of the anterior aspect of sacral nerve root tunnel on placement of iliosacral screw on the standard lateral sacral view.
METHODSFirstly the data of 2.0 mm slice pelvic axial CT images were imported into Mimics 10.0, and the sacrum, innominate bones, and sacral nerve root tunnels were reconstructed into 3D views respectively, which were rotated to the standard lateral sacral views, pelvic outlet and inlet views. Then the location and course of the S1, S2 sacral nerve root tunnel on each view were observed.
RESULTSThe sacral nerve root tunnel started from the cranial end and anterior aspect of the vertebral canal of the same segment and ended up to the anterior sacral foramen with a direction from cranial-posterior-medial to caudal-anterior-lateral. The tunnel had a lower density than the iliac cortex and greater sciatic notch on the pelvic X-rays,especially on the standard sacral lateral view, on which it showed up as a disrupted are line and required more careful recognition.
CONCLUSIONIt can prevent the iliosacral screw from penetrating the sacral nerve root tunnel and vertebral canal when recognizing the anterior aspect of sacral nerve root tunnel and choosing it as the caudal-posterior boundary of the "safe zone" on the standard lateral sacral view.
Adult ; Aged ; Bone Screws ; Female ; Fracture Fixation, Internal ; Fractures, Bone ; surgery ; Humans ; Male ; Middle Aged ; Pelvic Bones ; diagnostic imaging ; injuries ; innervation ; surgery ; Radiography ; Sacrococcygeal Region ; diagnostic imaging ; innervation ; surgery ; Sacrum ; diagnostic imaging ; injuries ; innervation ; surgery ; Spinal Nerve Roots ; diagnostic imaging ; surgery ; Young Adult
4.Construction of Salmonella Pullorum C79-13ΔcrpΔasd mutant balanced-lethal host-vector system and its biological features
Songbiao CHEN ; Jing LI ; Ke SHANG ; Chuan YU ; Chunjie ZHANG ; Xiangchao CHENG ; Yinju LI ; Zhanqin ZHAO
Chinese Journal of Immunology 2014;(8):1083-1087,1092
In order to develop a safer vaccine strain exploit Salmonella Pullorum vaccine strain as a live vaccine vector.Methods:AΔcrpΔasd mutant of S.pullorum C79-13 strain was constructed and it was developed E.coli donor strain mutant was generated through the two-step method introduced by χ7213 ( pREΔasd) was conjugated with the recipient of C 79-13Δcrp.The C79-13ΔcrpΔasd the transduction of recombinant suicide plasmid .Results:PCR and sequencing results showed that ΔsipBSL1344 was suc-cessfully constructed.The further study indicated that foreign DAP must be supplied for the ΔcrpΔasd mutant to grow,in addition,the asd gene was transmitted stably .Compared with C79-13 strain,the O antigens was identical to C79-13 strain,but the growth velocity was reduced significantly ,significantly reduced virulence .Conclusion: The ΔcrpΔasd mutant can accept asd+plasmid to construct host-vector balance lethal system , and this system can be used to express foreign gene efficiently and to develop potential oral multivalent vaccines.
5.Angiomyolipoma of the kidney with lymph node involvement.
Chuan-Zhen WU ; Feng-Hua WANG ; Cheng-Mei LI ; Wen-Chang FANG ; Jia-Ni YU
Chinese Journal of Pathology 2005;34(11):715-715
Adrenalectomy
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Adult
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Angiomyolipoma
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pathology
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surgery
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Follow-Up Studies
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Humans
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Kidney
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pathology
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Kidney Neoplasms
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pathology
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surgery
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Lymph Node Excision
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Lymph Nodes
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pathology
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Lymphatic Metastasis
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Male
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Nephrectomy
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Ureter
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surgery
6.Influence of needling at yin-yang meridian points on cerebral glucose metabolism.
Jing-cheng DONG ; Ji LI ; Chuan-tao ZUO
Chinese Journal of Integrated Traditional and Western Medicine 2002;22(2):107-109
OBJECTIVETo study the different effect of needling at different meridian points (MP) on different cerebral functional regions by using positron emission tomography and cerebral function tracer 18F-fluorodeoxy glucose to observe the effect of electro-acupuncture (EA) stimulation at Yin MP or Yang MP on local cerebral glucose metabolism in 30 healthy volunteers.
METHODSThe thirty subjects were randomly divided into 2 groups, the 12 subjects in the Yang MP group treated by needling at Hegu (LI4), Quchi (LI11), Zusanli (St36) and Shangjuxu (St37), the 18 subjects in the Yin MP group treated by needling at Neiguan (Pc6), Chize (Lu5), Sanyinjiao (Sp6) and Yinlingquan (Sp9). All the needling was conducted at one side of body.
RESULTSAfter needling at Yang MP, the cerebral glucose metabolism was elevated in bilateral cross region of frontal lobe and temporal lobe, parietal lobe, thalamus and basal ganglia, and in contralateral cerebellum and hippocampus. After needling at Yin MP, the cerebral glucose metabolism was lowered in bilateral cross region of frontal and temporal lobe, contralateral temporal lobe, cerebellum and thalamus, and was elevated in homolateral hippocampus and caudate nucleus.
CONCLUSIONUnder physiological condition, the regulatory effects of acupuncture on central nervous system would be different when needling at different MP.
Acupuncture Points ; Adult ; Brain ; metabolism ; Electroacupuncture ; Female ; Glucose ; metabolism ; Humans ; Male ; Meridians ; Middle Aged ; Random Allocation ; Tomography, Emission-Computed
7.Role of MafA gene in insulin production-Analysis of heterozygous knockout mice
Chuan ZHANG ; Bo LI ; Yan CHENG ; Yuhang YAO ; Lijuan SUN ; Satoru TAKAHASHI
Chinese Journal of Endocrinology and Metabolism 2013;29(12):1048-1051
Objective To clarify the role of MafA gene in development of MODY (maturity onset diabetes of the young) by studying insulin production,gene expression,and serum glucose level in heterozygous MafA gene knockout mice.Methods C57BL/6J mice were used as control animals,MafA gene heterozygous mice were analyzed.The distribution curve of blood sugar levels over time and serum insulin of heterozygous mice were determined by using IPGTT.The sensitivity of the mice to insulin was examined by injecting insulin assay.The expression levels of genes of MafA,insulin,pdX1,Beta2,and other genes of heterozygous mice were analyzed by semi-quantitative RT-PCR.Morphological changes in pancreatic tissue and α-and β-cell counts were obtained by using immunofluorescence/histological examination.Results (1) Two weeks after birth,MafA gene heterozygous mice began to show that the blood glucose level was increased,weight was reduced,and the amount of insulin secretion was clearly decreased (P<0.05 or P<0.01) while the insulin sensitivity did not change significantly.(2)The islet volume in MafA gene heterozygous mice was increased significantly as compared with the control group.However there were no significant changes in the number of pancreatic cells,distribution patterns,and the ratio of α and β cell.(3) Semi-quantitative RT-PCR detection showed that,compared with the control group,MafA gene level,the amount of insulin and Beta2 gene in MafA gene heterozygous mice were significantly reduced(all P<0.05),while no changes in the amount of glucagons and level of Pdx1 were found.Conclusions The blood glucose level of MafA gene heterozygous mice was raised early after birth.MafA gene is likely to be a new disease ralated gene of MODY.
8.Extraskeletal osteosarcoma of penis: report of a case.
Chuan-zhen WU ; Cheng-mei LI ; Wen-chang FANG ; Song HAN
Chinese Journal of Pathology 2011;40(9):640-640
12E7 Antigen
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Aged
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Antigens, CD
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metabolism
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Cell Adhesion Molecules
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metabolism
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Diagnosis, Differential
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Giant Cell Tumors
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pathology
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Giant Cells
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pathology
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Humans
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Male
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Mesenchymoma
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pathology
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Mucin-1
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metabolism
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Myositis Ossificans
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pathology
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Osteosarcoma
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metabolism
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pathology
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surgery
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Penile Neoplasms
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metabolism
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pathology
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surgery
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Vimentin
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metabolism
9.Roles of angiopoietin-2, Tie-2 and hypoxia-inducible factor 1alpha in angiogenesis of glioma.
Xiao-Han LI ; Xiao-Song WANG ; Cheng-Bo HAN ; Hua-Chuan ZHENG ; Xiang-Hong YANG
Chinese Journal of Pathology 2008;37(3):188-189
Adult
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Aged
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Angiopoietin-2
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physiology
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Female
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Glioma
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blood
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physiopathology
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Humans
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Hypoxia
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blood
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metabolism
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Male
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Middle Aged
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Neovascularization, Pathologic
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physiopathology
10.Hospital-acquired Gram-positive Infection in General ICU
Chuan ZHANG ; Bixia ZHENG ; Hui XIE ; Lidong JIANG ; Jinchuan CHENG ; Qi WEI ; Xiaoyu LI
Chinese Journal of Nosocomiology 2006;0(07):-
OBJECTIVE To investigate the characteristic of Gram-positive bacteria in general ICU then discuss the susceptible factors and the treatment.METHODS A retrospective analysis of clinical information was performed on patients with Gram-positive infection in ICU from May 2002 to May 2004.RESULTS Most of Gram-positive bacteria resulted in infection in general ICU were Staphylococcus aureus and all of them were MRSA.The infective site focused on lower respiratory tract(84.44%).The second was catheter(8.89%).CONCLUSIONS The risk factors of hospital-acquired infection are relative with patient's age,underlying diseases,stay time in ICU,ventilated time and invasive operation.