2.Suppression effect of RNA interference on expression of EGFR of SKOV3 cell line
lan, DAI ; wen, DI ; chuan-wei, DING
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(01):-
Objective To investigate the suppression effect of RNA interference(RNAi) on the expression of epidermal growth factor receptor(EGFR) of ovarian cancer SKOV3 cell line. Methods SKOV3 cells were transfected with synthetic EGFR sequence-specific dsRNA by lipofectamin 2000.The expression level of EGFR mRNA and protein were detected by RT-PCR and Western blotting,respectively.The proliferation of transfected SKOV3 cells was detected by WST-1 cell proliferation assay.The abilities of adhesion,migration and invasion were detected by cell adhesion assay,cell migration assay and Matrigel invasion assay,respectively. Results The suppression rates on EGFR mRNA and protein by sequence-specific dsRNA-EGFR were 73.65% and 58.94%,respectively.The cell proliferation was inhibited by 22.54%,35.76% and 31.07%,respectively at 24,48 and 72 h after transfection.The adhesion of sequence-specific dsRNA transfected SKOV3 cells for 30 min and 90 min were reduced by 12.11% and 18.66%,respectively.The migration and invasion of SKOV3 cells were decreased by 25.47% and 22.08%,respectively. Conclusion The down-regulation of EGFR expression of ovarian cancer by sequence-specific dsRNA can lead to the suppression of proliferation,adhesion,migration and invasion of ovarian cancer cells.
3.Erdheim-Chester disease in a child: case report.
Chuan WEN ; Qing-chun LIANG ; Wu-qing WAN
Chinese Journal of Pediatrics 2011;49(3):231-232
4.Hydroxyapatite ocular prosthesis implants wrapped by human amniotic membrane
Wenbin LIU ; Siqin SUN ; Yaochun WEN ; Chuan WAN
Recent Advances in Ophthalmology 2001;21(3):191-192
Objective To observe the clinical effects of human amniotic membrane as an alternative substrate for wrapping hydroxyapatite ocular prosthesis implants.Methods After enucleation, hydroxyapatite orbital implants wrapped by human amniotic membrane were implanted with four rectus sutured to the implants .Among them, 4 cases were given primary implantation and 3 cases secondary implantation.Results After 8 to 39 week follow-up, it was found that in 6 cases, the eyelids were plump and prosthesis moved well, but one implant was removed because of failure of scleral patch graft for implant exposure.Conclusion The clinical results were satisfactory for ocular prosthesis implantation of hydroxyapatite sphere wrapped by human amniotic membrane, which may be considered as an alternative substrate for ocular prosthesis implants wrapper.
5.Analysis on the Support Mode of the AIDS Community in British
Wen-Juan XUE ; Ming-Xu WANG ; Ping-Chuan ZHANG ;
Chinese Medical Ethics 1995;0(02):-
The care and support for the patient of AIDS is a vital content,we should enhance international collaboration and intercourse of information,which can promote efficiency.This text shows an analysis on the actuality and characteristic of the support mode of the AIDS community in British.Its purpose is to summarize the successful experience for china and improve the support mode for AIDS.
6.Idea of public relationship and the quality education of medical students
Wen LI ; Chuan ZHAO ; Yue Lü ; Jianping SI
International Journal of Traditional Chinese Medicine 2010;32(2):133-
Public relationship has an outstanding effect on virtue education. Public relationship can regulate behavior, culture noble ethics, coordinate interpersonal relations and improve the ability of society adaptation for medical students. Public relationship can also build up good image of medical students, and improve their comprehensive qualities.
7.MEASUREMENT OF DNA CONTENTS OF NORMAL AND CARCINOMATOUS CELLS IN SITU OF HUMAN MAMMARY GLAND
Jinyu XIE ; Mingfu LIU ; Weiquan HUANG ; Chuan WEN
Acta Anatomica Sinica 1953;0(01):-
Cell smears were made from fresh mammary tissue during operation. There are 32 samples with definite pathological diagnosis. DNA content was measured in interphase nuclei of single epithelial cells of lesions of the human breast by a microspectrophotometric technique. The normal lymphocytes from 2 cases of normal blood samples and from 4 cases of mammalopathic tissue were used as a reference for the DNA value corresponding to a diploid number of chromosomes. The normal lymphocyte had mean DNA value in 11.2?0.2 A.U. and 10.9?1.3 A.U. The rest of the measurements showed: 12.1?1.3 A.U. in the, epithelial nuclei of 10 cases of neighbouring mammalopathic tissue; 22.6?1.0 A.U. in 9 cases of fibrcma of the breast; 31.9?2.3 A.U. in 8 cases of carcinomas of the breast. The cell nuclei of carcinomas showed abnormal DNA values and the multiploid cells were dominant. It's histograms showed a shift to right and either a double-peak or multiploid-peak distribution. The DNA contents of the benign lesion cells were significantly lower than that of cancer cells, but higher than that of normal cells. The peak of their histogram showed they are between 2C-4C.
8.Comparison of the efficacy of diffusion-weighted imaging and PET-CT in diagnosis of nasopharyngeal cancer.
Hui LI ; Chuan-miao XIE ; Xue-wen LIU
Chinese Journal of Oncology 2011;33(10):791-792
Adult
;
Aged
;
Bone Neoplasms
;
diagnosis
;
secondary
;
Diffusion Magnetic Resonance Imaging
;
methods
;
Female
;
Follow-Up Studies
;
Humans
;
Liver Neoplasms
;
diagnosis
;
secondary
;
Male
;
Middle Aged
;
Multimodal Imaging
;
methods
;
Nasopharyngeal Neoplasms
;
diagnosis
;
Positron-Emission Tomography
;
Tomography, X-Ray Computed
;
Whole Body Imaging
;
methods
9.A Research on Culturing the Synthetic Ability of Medic by the Scientific After-class Activities in Pediatrics
Chuan WEN ; Qing GUO ; Xiuying WANG ; Zhuwen YI
Chinese Journal of Medical Education Research 2003;0(02):-
The scientific after-class activities in pediatrics can increase the synthetic ability of the medical students,including the manners of occupation,professional level,the skill of communication,clinical practice,serving community,the management of information and the critical thinking.
10.The Investigation and Thinking on Clinical Teaching Way in Pediatrics
Chuan WEN ; Qing GUO ; Xiuying WANG ; Dingan MAO
Chinese Journal of Medical Education Research 2006;0(10):-
Clinical teaching way in pediatrics has been investigated,and its current state understood to provide the direction of clinical teaching in pediatrics.