1.Progress in Neural Differentiation of Umbilical Cord Blood -Derived Stem Cells
Yun-Tao LI ; Qiao-Chuan LI ; Lu-Gui QIU ;
China Biotechnology 2006;0(06):-
The ability of repair and regeneration of central nervous system (CNS) is limited. So many researchers applied themselves to search a valuable cell resource for treating severe diseases of the CNS. Several studies from different laboratories have recently reported that stem cells derived from human umbilical cord blood under certain in vitro conditions can manifest neural features that resemble features of neural-derived cells. In vivo transplantation studies have shown that these stem cells persistently engraft in the CNS, some engrafted cells acquire the characteristics of neurons and glia, and improve functional recovery after central nervous system injury. The existence of stem/progenitor cells with previously unappreciated proliferation and differentiation potential in umbilical cord blood raise the possibility that cord blood may provide an efficient source of cells differentiating into the neural lineage, with a potential to be employed in the therapy of human CNS diseases. The achievement and focuses on the mechanisms and modulation of induction of differentiation and in vitro and in vivo studies in this field was reviewed.
2.The influence of Survivin in drug resistance of breast cancer cell.
Ping XIE ; Qiu-rong RUAN ; Jun-chuan LI
Chinese Journal of Pathology 2005;34(6):367-368
Antimetabolites, Antineoplastic
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pharmacology
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Apoptosis
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Breast Neoplasms
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genetics
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metabolism
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pathology
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Cell Line, Tumor
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Drug Resistance, Neoplasm
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Female
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Fluorouracil
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pharmacology
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Gene Expression Regulation, Neoplastic
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Humans
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Inhibitor of Apoptosis Proteins
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Microtubule-Associated Proteins
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biosynthesis
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genetics
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physiology
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Neoplasm Proteins
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biosynthesis
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genetics
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physiology
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Oligodeoxyribonucleotides, Antisense
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genetics
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RNA, Messenger
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biosynthesis
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genetics
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Transfection
4.Meningitis caused by Enterococcus casseliflavus with refractory cerebrospinal fluid leakage following endoscopic endonasal removal of skull base chondrosarcoma.
Ming-Chu LI ; Hong-Chuan GUO ; Ge CHEN ; Feng KONG ; Qiu-Hang ZHANG
Chinese Medical Journal 2011;124(20):3440-3440
Cerebrospinal Fluid Rhinorrhea
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diagnosis
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etiology
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Chondrosarcoma
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surgery
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Endoscopy
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Enterococcus
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pathogenicity
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Humans
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Male
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Meningitis
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diagnosis
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microbiology
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Middle Aged
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Skull Base
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pathology
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surgery
6.Development of human antibodies against the Gn protein of severe fever with thrombocytopenia syndrome virus.
Suhua CHEN ; Lina SUN ; Yang LIU ; Chuan LI ; Lin LIU ; Mifang LIANG ; Peihong QIU
Chinese Journal of Virology 2015;31(1):24-29
To obtain human antibodies against the Gn protein of Severe fever with thrombocytopenia syndrome virus (SFTSV) with phage display technology, this study aimed to screen anti-Gn protein antibodies from an anti-SFTSV Fab human phage display library. Antibody genes were identified by sequence analysis and the specificity of antibodies was confirmed by ELISA. The Fab antibody genes were cloned into the HL51-14 vector and expressed in a mammalian cell expression system. IgG antibodies were then purified by protein A affinity chromatography,and the results were further confirmed by ELISA,IFA,western blotting assays and micro-neutralization tests. The results showed that, after three rounds of panning, there were 390 human Fab antibodies against SFTSV particles, of which 364 were specific for nucleoprotein. Coated with the Gn protein, eight different Fab antibodies specific for Gn protein were obtained after the determination of the subtype and subclass of antibodies by gene sequencing; five of these antibodies were from the Lambda library and three were from the Kappa library. The eight IgG antibodies could specifically bind to Gn protein according to the ELISA, IFA and Western blotting assays. The micro-neutralization test showed that these eight antibodies had no neutralizing activity,but they could still provide a reference for research in human monoclonal antibodies against SFTSV.
Antibodies
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genetics
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immunology
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Bunyaviridae Infections
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genetics
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immunology
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virology
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Cell Line
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Cloning, Molecular
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Humans
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Immunoglobulin Fab Fragments
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genetics
;
immunology
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Immunoglobulin G
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genetics
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immunology
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Neutralization Tests
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Phlebovirus
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genetics
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immunology
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Viral Proteins
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genetics
;
immunology
7.Inclusion Bodies are Formed in SFTSV-infected Human Macrophages.
Cong JIN ; Jingdong SONG ; Ying HAN ; Chuan LI ; Peihong QIU ; Mifang LIANG
Chinese Journal of Virology 2016;32(1):19-25
The severe fever with thrombocytopenia syndrome virus (SFTSV) is a new member in the genus Phlebovirus of the family Bunyaviridae identified in China. The SFTSV is also the causative pathogen of an emerging infectious disease: severe fever with thrombocytopenia syndrome. Using immunofluorescent staining and confocal microscopy, the intracellular distribution of nucleocapsid protein (NP) in SFTSV-infected THP-1 cells was investigated with serial doses of SFTSV at different times after infection. Transmission electron microscopy was used to observe the ultrafine intracellular structure of SFTSV-infected THP-1 cells at different times after infection. SFTSV NP could form intracellular inclusion bodies in infected THP-1 cells. The association between NP-formed inclusion bodies and virus production was analyzed: the size of the inclusion body formed 3 days after infection was correlated with the viral load in supernatants collected 7 days after infection. These findings suggest that the inclusion bodies formed in SFTSV-infected THP-1 cells could be where the SFTSV uses host-cell proteins and intracellular organelles to produce new viral particles.
Cell Line
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China
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Humans
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Inclusion Bodies, Viral
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ultrastructure
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virology
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Macrophages
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ultrastructure
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virology
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Phlebotomus Fever
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virology
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Phlebovirus
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genetics
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physiology
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ultrastructure
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Thrombocytopenia
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virology
8.Research of Human-mouse Chimeric Antibodies Against Ebola Virus Nucleoprotein.
Rongping ZHOU ; Lina SUN ; Yang LIU ; Wei WU ; Chuan LI ; Mifang LIANG ; Peihong QIU
Chinese Journal of Virology 2016;32(1):14-18
The Ebola virus is highly infectious and can result in death in ≤ 90% of infected subjects. Detection of the Ebola virus and diagnosis of infection are extremely important for epidemic control. Presently, Chinese laboratories detect the nucleic acids of the Ebola virus by real-time reverse transcription-polymerase chain reaction (RT-PCR). However, such detection takes a relatively long time and necessitates skilled personnel and expensive equipment. Enzyme-linked immunosorbent assay (ELISA) of serum is simple, easy to operate, and can be used to ascertain if a patient is infected with the Ebola virus as well as the degree of infection. Hence, ELISA can be used in epidemiological investigations and is a strong complement to detection of nucleic acids. Cases of Ebola hemorrhagic fever have not been documented in China, so quality-control material for positive serology is needed. Construction and expression of human-mouse chimeric antibodies against the nucleoprotein of the Ebola virus was carried out. Genes encoding variable heavy (VH) and variable light (VL) chains were extracted and amplified from murine hybridoma cells. Genes encoding the VH and VL chains of monoclonal antibodies were amplified by RT-PCR. According to sequence analyses, a primer was designed to amplify functional sequences relative to VH and VL chain. The eukaryotic expression vector HL51-14 carrying some human antibody heavy chain- and light chain-constant regions was used. IgG antibodies were obtained by transient transfection of 293T cells. Subsequently, immunological detection and immunological identification were identified by ELISA, immunofluorescence assay, and western blotting. These results showed that we constructed and purified two human- mouse chimeric antibodies.
Animals
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Antibodies, Monoclonal
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genetics
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immunology
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Cloning, Molecular
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Ebolavirus
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genetics
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immunology
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Hemorrhagic Fever, Ebola
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immunology
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virology
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Humans
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Immunoglobulin Heavy Chains
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genetics
;
immunology
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Mice
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Nucleoproteins
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genetics
;
immunology
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Viral Proteins
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genetics
;
immunology
9.Safety of rush allergen-specific immunotherapy in allergic rhinitis patients.
Qianhui QIU ; Chuan LUC ; Jianjun CHEN ; Hong HAN ; Weijia KONG ; Shaohua CHEN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(11):776-784
OBJECTIVE:
The safety of rush immunotherapy (RIT) in Chinese allergic rhinitis (AR)patients is unknown. The purpose of this prospective was to assess the safety differences between RIT and conventional immunotherapy in Chinese AR patients, and then discuss the clinical application feasibility of RIT.
METHOD:
A one-year study period was set for this study. The enrolled patients were divided into 2 groups according to their preference of therapy: RIT or conventional immunotherapy using standardized house dust mite allergen vaccine. For safety evaluation, the local and systemic adverse reactions were recorded throughout the both groups initial phase. Week 0 (W0), Week 2 (W2), Week 5 (W5), Week 17 (W17) were set as observation time points for leukotriene (LT-B4) and so on. The Generalized Mixed Linear Model with SPSS13. O and the chi-square test with SAS 9. 1.3 were used for Statistics.
RESULT:
Fifty-two cases were enrolled into the RIT group, of which 49 patients have completed the established treatment study, and 3 cases were lost to follow-up. In the conventional immunotherapy group, 35 cases were enrolled, of which 32 have completed established treatment study, and 3 cases were lost to follow-up. The local and systemic adverse events of AR RIT appeared to be similar to those of conventional therapy and LT-B4 was descended steadily in the two groups.
CONCLUSION
Processed in advance Chinesear with drugs, RIT is similar to the safety of conventional immunotherapy.
Humans
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Immunotherapy
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methods
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Linear Models
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Prospective Studies
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Rhinitis, Allergic
;
therapy
10.Effect of EGF on expression of EGFR and EGF mRNA in the injuried lung tissue of neonatal premature rats exposed to hyperoxia
Guosheng LIU ; Haiying LIU ; Ruiqin QIU ; Chuan NIE ; Xiaopeng ZHAO ; Juling KANG ; Jiebin GUAN
Chinese Journal of Pathophysiology 2000;0(07):-
AIM:To investigate the effect of exogenous epidermal growth factor(EGF) on expression of EGFR and EGF mRNA of the injuried lung tissue of neonatal premature rats exposed to hyperoxia.METHODS:The neonatal Sprague-Dawley rats of 21-day gestational age model of hyperoxia induced lung injury were made by continually inhaling 95% oxygen.The model rats were divided into two groups randomly,the EGF trail group and the NS control group.The other rats were taken into the air control group.Each group was divided into three subsets:a(1-3 days),b(4-6 days) and c(1-6 days) according to different application times of EGF or NS.Rats in sub groups were executed and the lung tissues were removed at postnatal 3th,7th,14th day respectively.Using immunohistochemistry method,the expression of EGF-R of lung tissues in different groups was observed,and the expression of EGF mRNA was determined by reverse transcriptase polymerase chain reaction(RT-PCR).RESULTS:The expression of EGF mRNA increased by degrees following the increasing postnatal days.Compared with the air control group,the expression of EGF-R and EGF mRNA increased in the hyperxia group at 7th day and 14th day.The expression of EGF-R increased in corresponding hyperxia NS control group at 14th day(P