OBJECTIVE:To investigate the characteristics and regulation of antibiotics-induced ADR in our hospital and ex-plore effective prevention measures,in order to provide reference for safe and rational use of antibiotics in the clinic. METHODS:111 antiobiotics-induced ADR reports were collected from our hospital during 2012-2014,and analyzed in terms of patient’s gender and age,route of administration,drug category,organs/systems involved in ADR,etc. RESULTS:The patients aged <10 years old and >60 years old took up the highest proportion,accounting for 27.93% and 25.23%,espectively. ADR induced by intrave-nous route of administration had the highest incidence,accounting for 84.68%;cephalosporins-induced ADR was the most,account-ing for 26.13%;lesion of skin and its appendents was main antibiotics-induced ADR,accounting for 44.92%. CONCLUSIONS:Clinical pharmacists should provide technical support to doctors and nurses to aviod the occurrence of ADR.

Cancer is one of the major harmful diseases threatening human health and life. Development of angiogenesis inhibitors targeting tumor angiogenesis has become an important topic in the field of antitumor. Vascular endothelial growth factor (VEGF), a primary factor in growth and differentiation of vascular endothelial cell, plays an important role in angiogenesis. VEGF receptors include vascular endothelial growth factor receptor 1 (VEGFR1) and VEGFR2. VEGF/VEGFR2 signaling pathway is a critical pathway in regulating tumor angiogenesis. The amount of new blood vessels in tumor could be reduced and the proliferation, invasion, metastasis of tumor could be inhibited by treatment targeting VEGF/VEGFR. Traditional Chinese medicine possesses characteristic in the treatment of cancer, and it has huge potential in screening and developing tumor-angiogenesis inhibitors. Progress in research of natural products targeting VEGF/VEGFR was reviewed in this paper to provide reference for further study and development of these products.

Aged ; Hodgkin Disease ; pathology ; therapy ; Humans ; Lymphoma, Mantle-Cell ; Male ; Stomach Neoplasms ; secondary

Breath Tests ; methods ; Child ; Humans ; Infant ; Lung Diseases ; diagnosis ; physiopathology ; Lung Volume Measurements ; Maximal Voluntary Ventilation ; Respiratory Function Tests ; Tidal Volume

Aim To study the involvements of nuclear factor of activated T-cells (NFATc) and NFκB in calcineurin-mediated ischemic brain damage in vivo. Methods The rat transient forebrain ischemia conducted through 15 min ischemia followed by 8, 24, and 72 h reperfusion was induced using the fourvessel method. The rats were divided randomly into five groups; sham control group, ischemia/reperfusion (I/R) group, CsA treated groups (for 8, 24, and 72 h reperfusion). Western blotting was performed to detect changes of FasL, NFATc, I-κB-α, and phospho-I-κB-α protein expression, and gel shift assays for NFAT FasL-DNA binding activities. Results Western blotting showed that the expressions of both FasL and NFATc protein were significantly increased in the hippocanpus of rat subjected to transient forebrain ischemia in comparison with those of the sham control group, which were markedly reduced by CsA. The I-κB-α protein showed no changes in all groups, and phospho-I-κB-α protein was not observed in this study. Proximal and distal FasL promoter NFAT sites bind NFAT proteins from the hippocampal neurons subjected to transient forebrain ischemia, and DNA-binding activities increased significantly compared with those of the sham control group. CsA markedly inhibited these changes. Conclusion NFATc may be involved in calcineurin-mediated ischemic brain damage and transcription factor NF-κB may not be involved.

AIM To study the effect and mechanism of valdecoxib, a selective COX 2 inhibitor, on human gastric cancer BGC 823 cells. METHODS MTT assay and flow cytometry were used to observe the effect of valdecoxib on proliferation, apoptosis and the cell cycle distribution of BGC 823 cells. Laser confocal microscopy, transmission electron microscope and DNA fragmentation assay were further used to detect the apoptosis. The content of LDH was examined using LDH kit. RESULTS Valdecoxib in 25～400 ?mol?L -1 significantly inhibited the proliferation of BGC 823 cell in a time and dose dependent fashion, the inhibition rate of proliferation was 24 0%～92 0% after 72 h, and the rate of apoptosis was increased from (2 6?0 7)% to (7 6?1 5) %～(16 5?1 5)%. 100～400 ?mol?L -1 valdecoxib also decreased the proliferation index and the proportion of cells in the S phase, increased the proportion of cells in the G 0/G 1 phase, but had no effect on the proportion of cell in the G 2/M phase. CONCLUSION Valdecoxib inhibits human gastric cancer BGC 823 cells growth by inducing apoptosis and cell cycle arrest. The growth inhibitory effect of 400 ?mol?L -1 valdecoxib is also associated with cell necrosis.

Objective To investigate the relationship between blood concentration of sodium valproate and the anti‐epileptic effect and the influencing factors of blood concentration of sodium valproate ,and to provide evidence for clinical individual adminis‐tration .Methods The blood concentrations of 133 cases of patients treated with sodium valproate were determined in the affiliated Yongchuan hospital of Chongqing medical university ,the monitoring results of blood concentration were statistically analyzed ,and the anti‐epileptic effect of 133 cases of patients were observed and analyzed .Results The epilepsy of 80 cases of patients were con‐trolled ,accounted for 60 .15% of the total number;in the 69 cases of patients within the effective blood drug concentration (50-100 mg/L) ,the epilepsy of 51 cases of patients were controlled ,accounting for 38 .35% .In the 44 cases of patients whose blood drug concentration were less than 50mg/L ,the epilepsy of 26 cases of patients were not controlled .The distribution of blood concentra‐tion between men and women were similar .The blood concentrations among each age group were different ,the blood concentrations of 52 .87% patients in the adult group were below or above therapeutic range that were 39 .13% in the minor group .The adverse reactions were increase with the increase of blood concentration .Conclusion There are differences between the blood concentrations of sodium valproate and clinical effect ,the reasonable individual administration should be conducted according to the patient′s blood concentrations of sodium valproate ,the epilepsy control situation of patients and the patients′age .

Neuromyelitis optica (NMO) is a rare disorder in children with variable presentation. We report a 7-year-old boy who presented with bilateral retrobulbar optic neuritis and responded very well to treatment. He was also positive for aquaporin 4 (AQP4) antibodies, which is part of an emerging endophenotype within autoimmune neurological disorders in childhood.
Neuromyelitis Optica

Abstract: Mosquitoes are involved in the transmission of serious diseases such as malaria, dengue, chikungunya, yellow fever, Zika virus disease, and filariasis, and their prevention and control have always been a research hotspot. Currently, mosquito control methods mainly include physical control, chemical control and biological control. Physical control methods are environmentally friendly, but they are slow to take effect and have unsatisfactory control effects; although chemical control can quickly eliminate mosquitoes, it has been eliminated due to its high pollution, high residual, and easy drug resistance; biological control uses natural enemies or pathogens to kill mosquitoes and reduce their ability to transmit disease. Therefore, environmentally friendly biological control has become the main measure for controlling and preventing mosquitoes. In recent years, significant progress has been made in bacterial mosquito control agents, among which Bacillus thuringiensis has been the most extensively studied. Bacillus thuringiensis is a Gram-positive soil microorganism, which is the pathogenic bacterium of a variety of agricultural pests such as Lepidoptera, Coleoptera and Diptera. During the sporulation process, its strains produce a variety of insecticidal crystal proteins (ICPs) or δ-endotoxins with insecticidal activity against mosquito larvae. This review firstly introduces the crystal proteins of Bacillus thuringiensis, describes in detail the types and structures of crystal proteins in detail, and also reveals the mechanism of action of crystal proteins related to receptors.

Objective To study the genome DNA methylation in rheumatoid arthirits(RA)and the re- lated factors of DNA methylation.Methods Twenty-first cases with RA and 20 controls were recruited to par- ticipate the study.Plasma Hcy,SAM,SAH,the MTHFR gene C677T polymorphism and the expression of LFA-1 in CD4~+T cells was measured in all patients and controls.Results①The SAM levels were lower sig- nificantly in RA groups than in controls.The SAH levels were higher significantly in RA groups than in con- trols.②There was significant inverse correlation between plasma Hcy level and SAM level(r=-0.932,P＜0.01). There was significant positive correlation between plasma Hcy level and SAH level(r=0.924,P＜0.01).③The expression of LFA-1 in CD4~+T cells was higher significantly in RA groups than in controls.There was a signif- icant positive correlation between LFA-1 expression level and Hcy level(r=0.557,P＜0.01),a significant in- verse correlation between LFA-1 expression level and SAM level(r=-0.651,P＜0.01).④The MTHFR gene mu- tation lead to dramatically increase of Hcy,SAH level and the expression of LFA-1 level in CD4~+T cells and genome DNA hypomethylation.Conclusion①Hypomethylation of genome DNA is found in most RA pa- tients.②The factors associated with genome DNA hypomethylation include MTHFR gene mutation and hyper- homocysteinemia.③The expression of LFA-1 in CD4~+ T cells is higer in RA groups than in controls,which re- lates to the DNA methylation level and the MTHFR gene C677T polymorphism.