1.Identification of herbal tea ingredient Plumeria rubra and its adulterants using DNA barcoding.
Yu-Hua SHI ; Wei SUN ; Guang-Hong FANG ; Rong-Bo ZHENG ; Wen-Liu XU ; Xiao-Dan HUANG ; Shao-Quan WENG ; Chu-Yuan LI ; Shi-Lin CHEN
China Journal of Chinese Materia Medica 2014;39(12):2199-2203
ITS2 sequence was used as a barcode to identify herbal tea ingredient Plumeria rubra and its adulterants. Genomic DNAs from forty eight samples were extracted, the ITS2 sequences were amplified and sequenced bi-direstionlly, and then assembled and obtained using CodonCode Aligner. The sequences were aligned using ClustalW, the genetic distances were computed by kimura 2-parameter (K2P) model and the Neighbor-joining (NJ) phylogenetic trees were constructed using MEGA5.0. Results showed that the length of ITS2 sequence of P. rubra were 244 bp. The intra-specific genetic distances (0-0. 016 6) were much smaller than inter-specific ones between P. rubra and its adulterants(0.320 8-0.650 4). The NJ tree indicated that P. rubra and its adulterants could be distinguished clearly. Therefore, Using ITS2 barcode can accurately andeffectively distinguish herbal tea ingredient P. rubra from its adulterants, which providesa new molecular method to identify P. rubra and ensure its safety in use.
Apocynaceae
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classification
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genetics
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DNA Barcoding, Taxonomic
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methods
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DNA, Plant
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genetics
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DNA, Ribosomal Spacer
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genetics
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Drug Contamination
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prevention & control
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Drugs, Chinese Herbal
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chemistry
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classification
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Flowers
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chemistry
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classification
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Molecular Sequence Data
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Phylogeny
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Quality Control
3.Associations between partner-seeking activities through internet and sexual risk behaviours related to HIV/AIDS among men who have sex with men in China
Xiu-Fang LI ; Bei-Chuan ZHANG ; Quan-Sheng CHU ; Ning WANG ; Yan-Fei WANG ; Zeng-Zhao YU
Chinese Journal of Epidemiology 2008;29(7):685-688
Objective To explore the associations between partner-seeking activities through internet and HIV/AIDS related risk behaviours among men who have sex with men(MSM).Methods Target sampling.anonymous questionnaires were adopted and analyzed with SPSS 11.0.Results Data from 45.6% of the 2178 valid questionnaires showed that most partner-seeking activities was through internet.73.1% of them landed on gay website.in which 83.0% looked for sexual partners and 91.7% of them had intercourse with other men met through internet when compared with MSM who did not seek sex activities through internet.The internet group obviously had fewer numbers of partners(5.73 vs.10.3),oral sex partners(5.13 vs.8.71),passive anus sex partners(4.67 vs.8.26)and active anus sex partners (3.51 vs.6.06)in the past 6 months(P<0.01 respectively)and obviously had fewer group sex(16.7% vs.20.5%)activities in the past one year but had more activities on oral sex(94.7% vs.89.6%),anus sex(95.1% vs.86.5%),regular sexual partner(52.6% vs.46.7%)in the past 6 months(83.4% vs.75.5%)(P<0.01 respectively)and more likely to have finger intercourse(52.2% vs.38.5%),hemorrhage sexual intercourse(36.4% vs.21.9%,OR=2.04),non-local sexual intercourse(28.1% vs.22.7%)(P<0.01 respectively).Conclusion Seeking sexual partners through internet had positive and double effects on MSM's risk behaviours.suggesting that it is of urgent need to take the advange of internet and gay website to disseminate HIV/AIDS prevention message.
4.The impact of childhood sexual abuse on the development of AIDS related high risk behaviors and psychological appearances among men who have sex with men
Zeng-Zhao YU ; Bei-Chuan ZHANG ; Xiu-Fang LI ; Ning WANG ; Tong-Xin SHI ; Quan-Sheng CHU
Chinese Journal of Epidemiology 2009;30(1):14-17
Objective To study the AIDS related high risk behaviors and psychological appearances among men who have sex with men (MSM) who ever experienced childhood sexual abuse (CSA).Methods Target sampling for a cross-sectional study was developed and valid anonymous questionnaires were adopted to compare the differences of high risk behaviors related to AIDS and psychological appearances betwen those with or without CSA experiences among 2147 MSM from nine cities.Results Compared to corresponding ones without CSA experience,CSA group had a significant larger numbers in the following events: total sexual partners,anal sex episodes with same sex,female sexual partners and anal sex in the previous six months,with the figures of median as 20.0,10.0,3.0,3.0 respectively.In the previous year,30.8% of them had ever participated in 'group sex',19.2% ever exchanged money for sex,36.7% bled while having sexual intercourse,37.3% had sex with male partners away from his own region.All the above said figures were higher than non-CSA group,with significant differences.It also appeared that CSA experience had an impact on significant lower rate of condom use (67.3%) in the last anal sex.Those with CSA experience had more psychological problems which appeared as: 75.6% considered they would suffer from serious discrimination if their sexual orientation ever disclosed,34.7% had a strong intention of suicide and 24.3% ever having had suicidal attempts.The differences of the two groups showed statistical significance.Condusion CSA experience not only increased the number of AIDS related high risk behaviors in adulthood,but also had negative impact on their psychological appearances.It is of urgent need to carry out psychological intervention approaches to target on MSM with CSA experiences while childhood sexual education and rights assurance towards juvenile population should also not be neglected.
5.IL-13 R110Q, a Naturally Occurring IL-13 Polymorphism, Confers Enhanced Functional Activity in Cultured Human Bronchial Smooth Muscle Cells.
Ya Fang HE ; Li HUA ; Yi Xiao BAO ; Quan Hua LIU ; Yi CHU ; Ding Zhu FANG
Allergy, Asthma & Immunology Research 2013;5(6):377-382
PURPOSE: Interleukin (IL)-13, a Th2-type cytokine, plays a pivotal role in the pathogenesis of asthma through its direct effects on airway smooth muscles. A naturally occurring IL-13 polymorphism, R110Q, is strongly associated with increased total serum IgE levels and asthma. In the present study, we aimed to determine whether the IL-13 R110Q variant would display different biochemical properties or altered functions in comparison with wild-type (WT) IL-13 in cultured human bronchial smooth muscle cells (hBSMCs). METHODS: Culture supernatants and cell proteins were collected from cultured hBSMCs that were treated with 50 ng/mL IL-13 or IL-13 R110Q for 24 hours. Eotaxin released into hBSMC culture medium was determined by ELISA. The expression levels of the high-affinity IgE receptor (FcepsilonRI) alpha-chain, smooth muscle-specific actin alpha chain (alpha-SMA), smooth muscle myosin heavy chain (SmMHC), and calreticulin in the cells were measured on Western blots. RESULTS: Compared with WT IL-13, treatment with the IL-13 R110Q variant resulted in a significant increase in eotaxin release as well as significant, although modest, increases in the expression levels of alpha-SMA, SmMHC, calreticulin, and FcepsilonRI alpha-chain. CONCLUSIONS: The results of the present study suggenst that the IL-13 R110Q variant may enhance enhanced functional activities in hBSMCs.
Actins
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Asthma
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Calreticulin
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Enzyme-Linked Immunosorbent Assay
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Humans
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Immunoglobulin E
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Interleukin-13
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Interleukins
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Muscle, Smooth
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Myocytes, Smooth Muscle
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Myosin Heavy Chains
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Proteins
6.IL-13 R110Q, a Naturally Occurring IL-13 Polymorphism, Confers Enhanced Functional Activity in Cultured Human Bronchial Smooth Muscle Cells.
Ya Fang HE ; Li HUA ; Yi Xiao BAO ; Quan Hua LIU ; Yi CHU ; Ding Zhu FANG
Allergy, Asthma & Immunology Research 2013;5(6):377-382
PURPOSE: Interleukin (IL)-13, a Th2-type cytokine, plays a pivotal role in the pathogenesis of asthma through its direct effects on airway smooth muscles. A naturally occurring IL-13 polymorphism, R110Q, is strongly associated with increased total serum IgE levels and asthma. In the present study, we aimed to determine whether the IL-13 R110Q variant would display different biochemical properties or altered functions in comparison with wild-type (WT) IL-13 in cultured human bronchial smooth muscle cells (hBSMCs). METHODS: Culture supernatants and cell proteins were collected from cultured hBSMCs that were treated with 50 ng/mL IL-13 or IL-13 R110Q for 24 hours. Eotaxin released into hBSMC culture medium was determined by ELISA. The expression levels of the high-affinity IgE receptor (FcepsilonRI) alpha-chain, smooth muscle-specific actin alpha chain (alpha-SMA), smooth muscle myosin heavy chain (SmMHC), and calreticulin in the cells were measured on Western blots. RESULTS: Compared with WT IL-13, treatment with the IL-13 R110Q variant resulted in a significant increase in eotaxin release as well as significant, although modest, increases in the expression levels of alpha-SMA, SmMHC, calreticulin, and FcepsilonRI alpha-chain. CONCLUSIONS: The results of the present study suggenst that the IL-13 R110Q variant may enhance enhanced functional activities in hBSMCs.
Actins
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Asthma
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Calreticulin
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Enzyme-Linked Immunosorbent Assay
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Humans
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Immunoglobulin E
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Interleukin-13
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Interleukins
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Muscle, Smooth
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Myocytes, Smooth Muscle
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Myosin Heavy Chains
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Proteins
7.Clinical observation on the modified external fixation with splints for double fractures of forearm in children.
Chao WEN ; Hui-qin PAN ; Xing-sen LIANG ; Jian-bin WU ; Chu-quan FANG ; Shi-long XIE
China Journal of Orthopaedics and Traumatology 2010;23(8):624-625
OBJECTIVETo explore the advantages of conservative treatment for trauma of children,and evaluate effect of external fixation for the treatment of double fractures of forearm.
METHODSFrom June 2007 to June 2008, 52 patients with double fractures of forearm were treated with manipulative reduction and modified external fixation by splints. Among them, 33 males and 19 females,the age was from 4 to 15 years with an average of 8.6 years. The course of disease was from 1 hour to 7 days. All patients had a definite history of trauma, and pre-treatment X-ray film showed double fracture of ulna and radius.
RESULTSAll patients were followed up from 6 to 14 months with an average of 9.4 months. All fractures healed and the healing time was from 4 to 8 weeks with an average of 6 weeks. The clinical effect was evaluated according to Berton criteria, 37 cases obtained excellent result, 15 good.
CONCLUSIONStrict choose indication of conservative treatment can obtain satisfied effect in treating double fractures of forearm in children. The method has advantages of safe operation, less injury, cheap expense, which can well obtain functional rehabilitation of patients.
Adolescent ; Child ; Child, Preschool ; External Fixators ; Female ; Humans ; Male ; Manipulation, Orthopedic ; Radius Fractures ; surgery ; Splints ; Ulna Fractures ; surgery
8.Study on the association of partner-seeking and high risk behaviors related to AIDS among men who have sex with men in the different sites
Zeng-Zhao YU ; Tong-Xin SHI ; Xiu-Fang LI ; Yang LI ; Bei-Chuan ZHANG ; Quan-Sheng CHU ; Li-Xin WANG ; Min LI
Chinese Journal of Epidemiology 2010;31(6):642-646
Objective To explore the situation of AIDS-related high risk behaviors among men who have sex with men (MSM) ever experienced partner-seeking in different sites. Methods Target sampling for cross-sectional study ( "snowball" ) and valid anonymous questionnaires were adopted to compare the differences of high risk behaviors related to AIDS. 1834 MSM were studied in nine cities who sought sex partners through internet, bars or bath ponds. Results Compared to the other corresponding groups, those MSM from the bath pond group had a significant larger number of total sexual partners, chances of having anal and oral sex with same sex, and ones of anal sex in the previous six months with the median number as 40, 16, 20 and 4 respectively. Interact group had a higher rate of bleeding (36.4%) when having sexual intercourse in the previous year and a lower proportion of 8.7% exchanged sex for money, and 14.9% and 13.3% in both bar and bath pond groups respectively. Bar group had significant lower rates of condom use with 79.6% while having sex with men and 45.3% with women, but higher rates(28.9% ) ever participated in group sex in the last year and 4.2% with H1V infection. Conclusion MSM had high risk behaviors related to AIDS appeared in different sites when seeking partners. Intervention strategy should be taken, according to the characteristics of different subpopulations, especially on the bath pond group.
9.Shanghai community-based practice of early lung cancer screening with low-dose spiral computed tomography
Xiaoyang LUO ; Quan LIU ; Shengping WANG ; Yuan LI ; Lei SHEN ; Guodong LI ; Wentao LI ; Yanping ZHAO ; Huilin XU ; Hong FANG ; Guiqiang SHAO ; Jizhi CHU ; Junlei SUN ; Hongqi ZHU ; Zhiyong LI ; Lianghua JIANG ; Jianliang LING ; Weizhong ZHAO ; Jing WANG ; Xiaohua LIU ; Bin LI ; Yiliang ZHANG ; Ting YE ; Yunjian PAN ; Hong HU ; Rui WANG ; Yihua SUN ; Haiyan YANG ; Su XU ; Haiquan CHEN
China Oncology 2016;26(12):996-1003
Background and purpose:As one of the most fatal malignant tumors in China, the morbidity and mortality of lung cancer remain high. Early diagnosis and normative treatment is the key to improve the prognosis of lung cancer. The aim of this study was to explore the practice of early lung cancer screening with low-dose spiral computed tomography (CT) based on the current situation in community health service, with integration of superior resources of med-ical institutions at all levels in Shanghai.Methods:From Aug. 2013 to Aug. 2014, we screened high-risk population in selected communities of Minhang District, Shanghai, for early diagnosis of lung cancer with low-dose spiral CT combined with multidisciplinary comprehensive treatment models including minimally invasive surgery, exploring the medical service network covering prevention, diagnosis, treatment, rehabilitation and follow-up.Results:Screening population is 11 332 (male 7 144, female 4 188); Twenty-nine cases with pathological diagnosis of malignant tumor, including 27 cases of pri-mary lung cancer, 1 case of lung metastasis, 1 case of breast cancer. The morbidity of primary lung cancer is 238.26×10-5. There were 22 cases of Stage 0-Ⅰ lung cancer accounting for 81.48% of all diagnosed primary lung cancer.Conclusion:Based on community health service, screening with low-dose spiral CT could improve the early diagnosis rate of lung can-cer with feasibility and validity, which could be applicable in qualified eligible medical center and community in China.
10.Effect of Huangqi Guizhi Wuwutang on AGEs/RAGE/NF-κB Signaling Pathway in Diabetic Peripheral Neuropathy Rats
Ying FANG ; Ya-dong WANG ; Wen ZHOU ; Quan-gen CHU ; Wan-qiu HUANG
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(13):52-58
Objective:To study the effect of Huangqi Guizhi Wuwutang on receptor of advanced glycation end products(AGEs)/advanced glycation endproducts (RAGE)/nuclear transcription factor-kappa B p65 (NF-