Objective To establish a method for quantitative determination of 4 chemical constituentsgeniposide,genipin-1-β-D-gentiobioside,arctiin and baicalin in the effective fraction of Zhibangreduping Formula.Methods The procedare of HPLC was performed on the chromatographic column of Diamonsil C_(18)(4.6 mm×150 mm,5μm),and the mobile phase in the gradient elution was acetonitrile-phosphoric acid solution(0.2%).The flow rate was 1.0 mL/min and the detection wavelength was 238 nm and 278 nm.Results The average recovery of geniposide WaS 99.02%(RSD=1.99%,n=6),genipin-1-β-D-gentiobioside,99.03%(RSD=2.83%,n=6),arctiin,101.12%(RSD=2.86%,n=6),and baicalin,100.05%(RsD=2.02%.n=6).Conclusion The results of determination in 3 batches of samples show that the method is easy and accurate,which can be used in the quantitative determination of 4 chemical constituents in the effective fraction of Zhibangreduping Formula.

Objective To establish the characteristic chromatograms of Mongolian compound Sengdeng-4 in vivo and in vitro, analyze and identify its chemical components in vitro and serum. Methods After the model of rheumatoid arthritis was established, SD rats were orally administered Mongolian compound Sengdeng-4 and each single ingredient respectively. The medicinal serum were separated and measured with HPLC. The HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 in vitro and medicinal serum were identified. The characteristic chromatograms of Mongolian compound Sengdeng-4, single ingredient in vitro and medicinal serum were analyzed, and the chemical composition of Mongolian compound Sengdeng-4 in vitro, original crude medicinal in blood were identified and compared. Results The results of HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 showed that 47 peaks were identified. There were 14 chemical compositions absorbed into blood, among them 9 were original ingredients and 5 were metabolites. The second peak was from Wenguanmu; the fifth, sixth, and seventh peaks were from Zhizi; the first, eighth, and tenth peaks were from Hezi. The third peak was from Wenguanmu, Chuanlianzi and Hezi. The fourth and thirteenth peaks were from Mongolian compound Sengdeng-4. The ninth peak was from Wenguanmu and Chuanlianzi. The eleventh, twelfth
and fourteenth peaks were from Wenguanmu and Hezi. 7 components of Mongolian compound Sengdeng-4 were identified. Conclusion The chemical compositions and metabolites in blood may be the active ingredients with direct actions in vivo, which come from the ingredients of Mongolian compound Sengdeng-4 .