The purpose of this study was to investigate the effects of different Ca~(2+) concentrations on the ventricular fibrillation threshold(VFT) in the acute reg- ional ischemic isolated, paced rat hearts and to investigate their relations to the levels of cAMP, cGMP and ATP in the myocardium. It was demonstrated that a positive correlation was observed between [Ca~(2+)] and the percentage of VFT lowered in the ischemic hearts (r=0.7998, P

Protein Z (PZ) is a vitamin K-dependent protein. As a cofactor for the protein Z-dependent protease inhibitor (ZPI), it inhibits coagulation factor X under the existence of phospholipid and calcium ion, and increases the ZPI activity by nearly 1000-fold, thus it plays a role in the process of thrombosis. ZPI inhibits coagulation factor Ⅺ a alone. ZPI activity is also consumed in the process of inhabiting factor Ⅹa and Ⅺa. This article reviews the biological characteristics of PZ and ZPI and their association with stroke.

Objective: To explore the effect of selenium-protein polysaccharide extracted from Qinba (selenium-mushroom)(秦巴硒菇) on apoptosis of K562 cells.Methods: Tumor-bearing mice model was established,and selenium-protein polysaccharide extracted from Qinba selenium-mushroom contained-herb serum was prepared by the method of serum pharmacology.K562 cells were conventionally cultured.Growth inhibition rate of tumor cells was detected by methyl thiazolyl tetrazolium(MTT) method.Morphological changes of apoptotic cell were observed under a fluorescent microscope.Cell apoptosis was observed by DNA electrophoresis.Caspase3 relative gene was measured by colorimetry.Results: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could significantly inhibit the growth of K562 cells,there was a remarkably positive correlation between drug concentration,time and inhibitory rate.Apoptotic phenomenon was certained via morphological examination and DNA electrophoresis.Compared with control group,caspase3 gene was markedly upregulated.Conclusion: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could induce apoptosis of K562 cells.The mechanism may be related to upregulation of caspase3.

OBJECTIVE: To summary the application of new types of nanoparticles carriers emerged in recent years in drug or gene delivery. METHODS: By sorting, analyzing and summarizing domestic and foreign literatures, the characteristics, in vivo and in vitro properties and the applications in pharmacy of novel nanoparticles carriers such as nano cochleates, virus-like particles, hydrogel nanoparticles, gold nanoshells, carbon nanomaterials, quantum dots and dendrimers were reviewed and elaborated. RESULTS AND CONCLUSION: Nanoparticles possess special physical and chemical properties which could improve the stability and bioavailability of drugs and have a targeting and sustained release effect.

Objective To understand the incidence and trend of malaria in Xuzhou City so as to provide the evidence for formulating the malaria control measures. Methods The information of network reported malaria cases and epidemiological da-ta were collected and analyzed statistically in Xuzhou City from 2010-2013. Results A total of 109 malaria cases were reported by the special report systems and the network report system in Xuzhou City from 2010 to 2013 in which there were 44 cases 40.37% of vivax malaria 62 cases 56.88% of falciparum malaria 2 cases 1.83% of quartan malaria 1 case 0.92% of ovale malaria. The latter three were all imported from other countries. Totally 93 cases 85.32% were confirmed by laboratory and other 16 cases 14.68% were diagnosed clinically. There was the incidence throughout the year and there were no obvious seasonal characteristics. The positive rate of blood test in the floating population was significantly higher than that of the local res-idents χ2 868.23 P＜0.01 . Conclusion The malaria endemic situation is in the steady decline period in Xuzhou City. The local infections decrease significantly but the imported falciparum malaria cases increase year by year. Therefore the manage-ment for floating population and fever patients should be strengthened.

The human tissue kallikrein(KLK)gene family consists of 15 highly conservative serine pro-teases,which is the largest uninterrupted cluster of protease genes in the human genome. Several members of the family are expected to be markers for tumor diagnosis and prognosis. Studies find that the expressions of KLK2-11 and KLK13-15 are abnormal,and the majority of KLKs have potential diagnostic and prognostic values.

Objective To evaluate the clinical value of serum high density lipoprotein cholesterol (HDL-C)in the evaluation of synthetic function of the patients with liver diseases.Methods Abbott Aeroset automatic biochemical analyzer was used to detect serum HDL-C level in control group (30 cases) and liver disease group(87 cases).T test was used to analyze the differences of serum HDL-C level in liver disease group and control group,different liver disease groups and control group,and different liver disease groups.Results HDL-C level was (1.28 ±0.20)mmol/L in the control group,(0.77 ± 0.15)mmol/L in the liver disease group,and (0.81 ± 0.13)mmol/L in the hepatitis group,(0.68 ± 0.14)mmol/L in the liver cirrhosis group,(0.54 ± 0.05)mmol/L in the liver cancer group.The HDL-C level in liver disease group and hepatitis group,liver cirrhosis group,liver cancer group was significantly lower than that in the control group(t =15.5569,14.2463,45.4393,25.6344,all P ＜ 0.01).The HDL-C level in liver cirrhosis group and liver cancer group was significantly lower than that in hepatitis group (t =3.6583,16.9057,all P ＜ 0.01).The HDL-C level in liver cancer group was significantly lower than that in liver cirrhosis group(t =4.4103,P ＜ 0.01).Conclusion Serum HDL-C level can be used to evaluate synthetic function of the liver,the lower of serum HDL-C level,the more serious liver synthesis function is impaired.

Purpose:To assess the security and efficacy of mechanical recanalization and stenting of lilac arteries with complete occlusions without preceding thrombolytic therapy. Materials and methods:During a 3-year period,Eighteen consecutive patients underwent mechanical reeanalization and stenting for complete occlusion of the iliac artery.The method involved recanalizition with a guide wire and a catheter advanced as a while unit through the occluded segment(snowplow technique).Results The occluded segments were successfully traversed and dilated and 32 stents were placed in 18 patients.The mean ankle-brachial index (BAI)increased from 0.39?0.33 before the procedure to 0.86?0.13 after the procedure(P

Objective To assess the clinical history and MRI findings of pituitary adenoma of different hormonesecreting type.Methods We retrospectively studied 200 postoperated patients,from 1996 to 2000,who all had the preoperated MRI exams.All cases had histopathological evalutions.Both clinical presentation(age,sex,symtom,sign)and MRI findings(size,form,signal intensity,extension)were investigated.Results There was a great span of age with sixty percent owing for 35～55 years old.The male and female proportion was 1∶2 in PRL hormone-secreting pituitary adenoma.12 cases with luleinizing hormone-secreting and 2 cases with thyrotropin hormone-secreting pituitary adenoma were just male.In other types the incidences were similar in man and woman.Clinical symptom and sign present overlapping.The volume of tumor was from 0.5 cm?0.8 cm?1 cm to 6 cm?7 cm?8 cm with most multihormone adenoma's maxium dimension out of 5 cm.The signal intensity was not characteristic pre-and post-enhancement.No calcification could be seen.In term of extension,most was to suprasellar,then infrasellar,unilateral or bilateral sinus cavernous,last was to lower part of the third ventricle.Conclusion Different hormonesecreting pituitary adenomas have some speciality in clinical and MRI presentation.Although an accurate diagnosis can not be made according to it,more information have been obtained.

Objective To investigate the effect of nerve growth factor (NGF) and ciliary neurotrofic factor (CNTF) on the regeneration and functional recovery of sciatic nerves following sciatic nerve injuries (SNI). Methods The left sciatic nerves for 6 mm in 120 Wistar ratswere removed and divided randomly into 4 groups. Target muscular injection of NGF at concentration of 100 ng/kg and CNTF at 100 ng/kg was given to Group NGF+CNTF; CNTF at 100 ng/kg to Group CNTF; NGF at 100 ng/kg to Group NGF and equal quantity of normal saline to Group NS. Sciatic nerve functional index (SFI) measurement, morphometric analysis, S-100? and NF200 immunohistochemistry were performed for all the animals after operation. Results SFI, diameter and number of myelinated axons, and axonal quantity of S-100? and NF200 were significantly higher in Group NGF+CNTF than that in other groups. Conclusions Combined application of NGF and CNTF can enhance regeneration and functional recovery of sciatic nerves posterior to SNI.