China ; Humans ; Immunization Programs ; Poliomyelitis ; prevention & control ; Poliovirus Vaccines ; administration & dosage

The purpose of the present study is to demonstrate the distribution of carbonic anhydrase pattern in the various termatodes (Fasciola gigantica, Paramphistoma orthocoelium, Paragonimus westermani) by means of Kurada staining method, and to correlate these findings with the histochemical data and harboring location. The results are summarized as follows: In Fasciloa gigantica, carbonic anhydrase activity was positive in the vitelline gland cells and eggs in the uterus. In Paramphistoma orthocoelium, carbonic anhydrase activity was positive in the vitelline gland cells and eggs in the uterus. In Paragonimus westermani, carbonic anhydrase activity was positive in intestinal mucous membrane, vitelline gland cells and eggs.
parasitology-helminth-trematoda ; Fasciola gigantica ; Paramphistoma orthocoelium ; Paragonimus westermani ; histochemistry ; carbonic anhydrase

Objective To establish the chromatographic fingerprint of Curcuma wenyujin by HPLC.Methods The chromatographic fingerprint of C.wenyujin was established by HPLC with Shimadzu VP-ODS C18 column(250 mm?4.6 mm,5 ?m),acetonitrile-water(0.1 phosphoric acid) gradient elution,the flow rate of 1.0 mL/min,column temperatures of 30 ℃,and detective wavelength at 210 nm.ResultsTaking germacrone as the reference peak,14 common-peaks were selected as the fingerprint peaks.The similarities among samples of C.wenyujin among 19 approved samples and their standard chromatographic fingerprints were calculated with a software "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" published by GPC(Version 2004A) and cluster analysis.ConclusionThis method with better accuracy and reproducibility could be used in the quality control of C.wenyujin.

In this mini review,some research advance on Marinomonas from domestic and overseas was briefly summarized,mainly including of its classification、ecological distribution、functional genes and bioactive molecules.Furthermore,some suggestion and perspectives for further studies on Marinomonas were also proposed.

Objective To explore the epidemiologic and clinical features of hand, foot and mouth disease (HFMD) caused by Coxsackie virus A16 (CA16) in Suzhou from 2010 to 2014, and analyze the relationship between the SNPs of oligoadenylate synthetase 1 (OAS1) and HFMD caused by CA16 infection.MethodsThe clinical data of children diagnosed with HFMD caused by CA16 during 2010 and 2014 were collected. The epidemiological characteristics were analyzed. Among them, 167 cases were selected to make comparison of the clinical features with 166 cases of HFMD caused by EV71 infection in the same period. The genotyping ofOAS1 rs10774671 was detected by TaqMan probe technique in 167 cases of CA16 infection children, 166 cases of EV71 infection children with HFMD and 163 healthy children. The relationship between polymorphism of gene and infection of CA 16 was analyzed.ResultsA total of 9 016 children with HFMD were included. CA16 nucleic acid detected to be positive in 762 cases. The detection rate was 8.45%. CA16 infection was most commonly in summer. Children under 5 years old accounted for 94.62% infected. Compared with EV71 infected children, CA16 infected children had shorter fever time, severer oral herpes, ulcer, and rash in hand, foot and hip, lesser nervous system involvement, fewer cases of high lactate dehydrogenase, high C reactive protein, high IgM or IgG, and signiifcant changes in the percentage of CD3+, CD3+CD4+, CD3+CD8+ and CD3-CD19+ (P all?

No abstract available.
Flow Cytometry* ; Humans* ; Leukocytes* ; Microscopy* ; Phagocytosis* ; Staphylococcus aureus* ; Staphylococcus*

No abstract available.
Flow Cytometry* ; Humans* ; Leukocytes* ; Microscopy* ; Phagocytosis* ; Staphylococcus aureus* ; Staphylococcus*

The evaluation of the effects of anthelmintic drug dose was done by the eggs reduction and the eggs negative conversion rate. The drug dose according to the age of the patient was estimated by the formula of Augsberger, the dose according to the body weight of patient was estimated by the Rule of Six, and the dose to the body surface of the patient was estimated by the formula of Costeff. It is considered that the application of the drug dose according to the age of the patient resulted in reduction of dose in the younger patient, and resulted in the excess dose in older patient. The application of the drug dose according to the body weight also resulted in the reduction of dose in the lighter patient and the excess dose in the heavier patient. It is suggested that the application of drug dose according to the body surface is more reasonable than those by the age and the body weight. The value of this application was also proved to be medium value between those of age method and body weight method.
parasitology-helminth-nematoda-Ascaris lumbricoides ; pyrantel pamoate ; piperazine adipate ; santonin-kainic acid complex ; chemotherapy ; drug evaluation ; body weight ; body surface ; age

AIM: To elucidate the relationship of inflammation, coagulation and cholesterol gallstone formation. METHODS: Hamsters were divided into four groups: control group (feeding normal diet), lithogenic diet (LD) 2 weeks group, LD 6 weeks group and LD+aspirin 6 weeks group. Gallstone incidence, antithrombin antigen (AT-Ⅲ:Ag), antithrombin activity (AT-Ⅲ:Ac), thrombin (F-Ⅱa:Ac), plasminogen activator inhibitor activity (PAI:Ac), plasmin activity (Plm:Ac), D-dimer:Ag and C-reactive protein (CRP) in gallbladder bile were observed as read-out parameters. RESULTS: The incidence of gallstones in control group, LD 2 weeks group, LD 6 weeks group and LD+aspirin group were 0%, 20%, 73% and 25%, respectively. AT-Ⅲ:Ag, F-Ⅱa:Ac, D-dimer and CRP in LD 2 weeks group and LD 6 weeks group were significantly higher than those in the control group (P

The conductivity and permittivity of blood in mice were measured by the AC electrical impedance method at frequency range of 0.1-100MHz, and then the changes of the Cole-Cole parameters of dielectric spectra of blood from phenylhydrazine-induced anemia mice were observed by numerical calculation and curve fitting residual analysis of the Cole-Cole equation. The results showed that hematocrit (Hct) of the mice with phenylhydrazine injection was significantly reduced; the permittivity(epsilon) spectroscopy of blood moved to the low insulating region and its permittivity decreased; conductivity (kappa) spectrum curve of blood moved to the high conductivity zone and conductivity increased; the 2nd characteristic frequency was lower than that in the normal group. There was phenylhydrazine dose dependent in the changes of the Cole-Cole parameters of dielectric spectra of blood.
Anemia ; blood ; Animals ; Blood Physiological Phenomena ; Electric Conductivity ; Electric Impedance ; Hematocrit ; Mice