Objective To find out the efficacy of the combination therapy of the West and Taditional Chineses Medicine for acute appendicitis patients with postoperative diarrhea.Methods According to the digital table,109 acute appendicitis patients with postoperative diarrhea were randomly divided into 59 cases of the treatment group and 50 cases of the control group.The control group were given with the conventional therapy and Western medicine therapy,and the treatment group were given with the Traditional Chinese Medicine based on the control group.Test results of stool specimens pathogen,the efficacy and the occurrence of adverse drug reactions were observed.Results 96 pathogens were detected in stool specimens of the two groups of patients,the four pathogens with thelargest constituent ratios were Vibrio,Shigella,Aeromonas and Proteus,and the constituent ratio were respectively 36.5 ％,22.9％,18.8％ and 11.5％.The remarkable effective rate and total efficiency of the treatment group was 83.1％,96.6％,respectively,which were significantly higher than 58.0％,84.0％ of the control group(x2 =8.426,4.598,all P ＜ 0.05).The incidence of adverse drug reactions of treatment group and the control group were 5.1％,6.0％,respectively,there was no significant difference between the two groups (x2 =1.028,P ＞ 0.05).Conclusion The efficacy of the combination therapy of the West and Waditional Chineses Medicine for acute appendicitis patients with postoperative diarrhea was significant effect,and the incidence of adverse drug reactions was less.

Objective To investigate the correlation between serum S100B, IL-6 and intracranial pressure in patients with severe craniocerebral injury.Methods 81 cases of patients with severe brain injury in our hospital from August 2012 to April 2016 were selected,intracranial pressure was monitored immediately after admission to calculate the average daily ICP level ,and venous blood were collected after craniocerebral injury 6,12,24,48, 72 hours.Detection of serum S100B protein and IL-6 levels,and the correlation analysis with the level of intracranial pressure.Results Patients with severe craniocerebral injury S100B levels increased gradually after injury, reached the peak at 24 hours, then decreased gradually; while patients with IL-6 and intracranial pressure after injury gradually increased, the difference was statistically significant in different time points among the S100B,IL-6 and intracranial pressure levels (P<0.05).Conclusion The changes of intracranial pressure after severe craniocerebral injury were proportional to the levels of serum S100B and IL-6,S100B and IL-6 can reflect the changes of intracranial pressure,intracranial pressure changes predicted by S100B plasma concentration in 48 hours were more sensitive than those in the same concentration of IL-6.

AIM: To investigate relevant factors of stem cell transplantation for treating diabetic foot. METHODS: Disposal, outcome, adjuvant method and safety during stem cell transplantation for treatment of diabetic foot were analyzed. RESULTS: ①After autologous stem cells were depurated at a low temperature for 24-48 hours, number of malignant stem cells decreased. Compared to simple stem cell transplantation, tissue regeneration and neovascularization increased after adding fibrin base during transplantation. ②Ankle-brachial index and transcutaneous oxygen pressure were obviously elevated, rest pain and intermittent lameness were improved, new collateral circulation appeared, and arterial blood flow increased in patients undergoing bone marrow stem cell transplantation in the treatment of ischemic disease of lower extremity. ③Chinese medicine for supplement- ing qi and activating blood circulation for stem cell transplantation fully played regulatory effects of biological responses. Adjuvant methods such as ultrasound ablation in combination of stem cell transplantation could significantly increase lower collateral circula- tion of broken popliteal artery. ④No significant adverse reaction or severe outcome was detected in autologous stem cell transplan- tation in a short term. CONCLUSION: ①Autologous stem cells after purified by low temperature can effectively purify malignant stem cells in bone marrow stem cell transplantation. Transplanted outcome can be elevated by adding fibrin. ②Adjuvant methods can enhance outcome of stem cell transplantation. ③Stem cell transplantation for treating diabetic foot has obtained satisfactory outcomes, but some problems still exist.

Objective To assess the clinical history and MRI findings of pituitary adenoma of different hormonesecreting type.Methods We retrospectively studied 200 postoperated patients,from 1996 to 2000,who all had the preoperated MRI exams.All cases had histopathological evalutions.Both clinical presentation(age,sex,symtom,sign)and MRI findings(size,form,signal intensity,extension)were investigated.Results There was a great span of age with sixty percent owing for 35～55 years old.The male and female proportion was 1∶2 in PRL hormone-secreting pituitary adenoma.12 cases with luleinizing hormone-secreting and 2 cases with thyrotropin hormone-secreting pituitary adenoma were just male.In other types the incidences were similar in man and woman.Clinical symptom and sign present overlapping.The volume of tumor was from 0.5 cm?0.8 cm?1 cm to 6 cm?7 cm?8 cm with most multihormone adenoma's maxium dimension out of 5 cm.The signal intensity was not characteristic pre-and post-enhancement.No calcification could be seen.In term of extension,most was to suprasellar,then infrasellar,unilateral or bilateral sinus cavernous,last was to lower part of the third ventricle.Conclusion Different hormonesecreting pituitary adenomas have some speciality in clinical and MRI presentation.Although an accurate diagnosis can not be made according to it,more information have been obtained.

Objective To obtain magnetic resonance imaging(MRI) symptoms that could be used in medical diagnosis and identification by analyzing the diverse MRI appearance of brain multiple sclerosis(MS).Methods MRI findings of 41 samples of clinically definite MS, including the numbers, distributions, sizes, and shapes of the lesions were analyzed. The symbolic characteristics and enhanced expression are also covered.Results Brain MS might happen singly and multiply, and more frequently with multiple happening. In a multiple case, 4—15 lesions were observed the most. Some samples showed with diffuse lesions which were unable to be counted accurately presenting as a “dirty-appearing white matter”. The distribution of the lesions was observed most frequently at the two sides of periventricle, and following by the subcortical, corpus callosum, and brainstem. Only two lesions were observed at cerebrum. The size of most lesions range from a few millimeters to 2 cm, accounting for about 75% of the total samples. Lesions with size above 2 cm were seen seldomly, with the biggest one of 6—7 cm. According to the shapes and symptoms, lesions might be categorized as an acute and chronic ones. The acute lesions had a shape of oval or circle, with a swelling appearance, low signal of T_1WI, and isointensity or a slightly higher signal on the circle. T_2WI showed a high signal, with different increase and showing “core+lunar” sign. This kind of lesions showed an enhancement with the circle enhancement as the most typical one, which had a complete circle or non-conplete arc shape enhancement, even the big lesions. The chronic lesion might also be divided into two categories. One was the quasi-symmetric lesion, with the distribution along two sides of periventricle and with the shape of small puncture/patch, part of them merged into the big patch; another kind of lesions was found scattered sparsely at frontal, parietal lobes and two sides of the periventricle, with small patches in shape. The chronic lesions had an appearance of shrinking, sharp edge, even signals, and had no lunar circle phenomena and obvious enhancement.Conclusion Brain MS have diverse MRI appearence, with some of which are unique and symbolic.

Objective To evaluate the role of c?Jun N?terminal kinase ( JNK) and p38 mitogen?ac?tivated protein kinase ( p38MAPK) signaling pathways in attenuation of myocardial ischemia?reperfusion ( I∕R) injury by morphine postconditioning. Methods Healthy adult male Sprague?Dawley rats, weighing 180-240 g, were used in the study. Their hearts were excised and retrogradely perfused in a Langendorff apparatus with Krebs?Ringer ( K?R) buffer saturated with 95% O2?5% O2 at 37℃. After 15 min of equili?bration, 52 isolated hearts were divided into 4 groups ( n=13 each) using a random number table: control group (group C), I∕R group, morphine postconditioning group (group MP), and morphine postcondition?ing plus anisomycin group ( group MP+A) . The hearts were continuously perfused with K?R buffer for 105 min in group C. In group I∕R, the hearts were subjected to 45 min of global ischemia by stopping perfusion with K?R buffer, followed by 60 min of reperfusion by restoration of perfusion with K?R buffer. In group MP, the hearts were subjected to 45 min of global ischemia, followed by 10 min of reperfusion with K?R buffer containing 3?0 μmol∕L morphine and then by 50 min of reperfusion with K?R buffer. In group MP+A, the hearts were subjected to 45 min of global ischemia, followed by 10 min of reperfusion with K?R buffer containing 3?0 μmol∕L morphine and 1?0 μmol∕L anisomycin ( an activator of JNK and p38MAPK) and then by 50 min of reperfusion with K?R buffer. At 60 min of reperfusion, 8 hearts in each group were selected for measurement of the myocardial infarction and amount of creatine kinase?MB ( CK?MB) released from the myocardium, and the myocardial infarct size was calculated. At 20 min of reperfusion, 5 hearts in each group were selected to detect the expression of phosphorylated JNK ( p?JNK ) , phosphorylated p38MAPK ( p?p38MAPK) and cytochrome c ( Cyt c) in myocardial tissues ( by Western blot) and content of nicotinamide adenine dinucleotide ( NAD+) in myocardial tissues ( by spectrophotometry ) . Results Compared to group C, the myocardial infarct size and amount of CK?MB released from the myocardium were significantly increased, the expression of p?JNK, p?p38MAPK and Cyt c was significantly up?regulated, and the content of NAD+ was significantly decreased in I∕R, MP and MP+A groups ( P<0?05) . Compared to group I∕R, the myocardial infarct size and amount of CK?MB released from the myocardium were signifi?cantly decreased in MP and MP+A groups, and the expression of p?JNK, p?p38MAPK and Cyt c was sig?nificantly down?regulated, and the content of NAD+ was significantly increased in group MP (P<0?05). Compared to group MP , the myocardial infarct size and amount of CK?MB released from the myocardium were significantly increased, the expression of p?JNK, p?p38MAPK and Cyt c was significantly up?regula?ted, and the content of NAD+ was significantly decreased in group MP+A (P<0?05). Conclusion The mechanism by which morphine postconditioning attenuates myocardial I∕R injury is related to inhibition of activation of JNK and p38MAPK signaling pathways in rats.

AIM:To estimate the toxicological characterization of Jiutong Capsule(Radix Puerariae lobatae,Fructus seu Semen Hoveniae,etc.).METHODS:The acute toxicity test,micronucleus test of bone marrow cell,sperm shape abnormality test in mice,the Ames test,and 30 days feeding test in rat were performed.RESULTS:(1) The acute toxicity test showed that LD_ 50 of Jiutong Capsule was more than 10.0 g/kg.(2) The result of Ames test,micronucleus test of bone marrow cell,and sperm shape abnormality test were negative.(3) The 30 days feeding test showed that Jiutong Capsule had no cumulate toxicity in mice.CONCLUSION:The results show that Jiutong Capsule does not have toxicity,and it can't cause mutation and heredity toxicity.

Objective To evaluate the impact of donor age on graft short-term outcome after right lobe adult living donor liver transplantation (LDLT).Method Thirty living recipients were divided into 2 groups according to donor age:older donor group (O group,donor age ＞50,n =5) and younger donor group (Y group,donor age ＜30,n =25).Alanine transarninase (ALT),aspartate transaminase (AST),total bilirubin (TB),prothrombin time (PT) on the postoperative day 1 to 5 and graft liver regenerative ratio (LRR) on the postoperative month 0.5,1,3,and 6 between 2 groups were determined and compared.Result TB on postoperative day 1,2,3,4 and 5 was significantly higher in O group than in Y group there were significant differences between two groups (P＜0.01).There was no significant difference of other liver function parameters 2 groups (P ＞ 0.05).There was no significant difference in LRRs on the postoperative month 0.5,1,3 and 6 months postoperatively between 2 groups after LDLT (P＞0.05).Conclusion Although allografts from older donors in LDLT have prolonged jaundice than those of their younger counterparts,after strictly preoperative evaluation,donor age has little effect on short-term outcomes of graft after LDLT.

Objective To evaluate graft regeneration and influencing factors after right lobe adult living donor liver transplantation (LDLT) using MSCT.Method Sixty-three living recipients were included in this study.We measured graft volume periodically by using MSCT and IQQA-Liver workstation.The liver regenerative ratio (LRR) of different stages of recipients after LDLT was calculated and compared,and stepwise regression analysis was done to set up the regression equation.Result Within 0.5 month after LDLT,graft volume was increased rapidly,and LRR reached maximum [(106.11 + 30.90)％],then decreased slowly.There was significant difference in LRR among 0.5,1 month and 3,6 months after LDLT (P＜0.05).The following factors,including whether the graft containing middle hepatic vein or not,age and sex of recipients,had no significant influence on LRR after LDLT (P＞0.05).The status of liver function of recipients preoperatively had significant influence on LRR 0.5 month after LDLT (P＜0.05).There was significantly negative correlation between the graft volume and LRRs of recipients at different stages after LDLT (P ＜ 0.05).Regression equation could be derived.Conclusion Most evident graft regeneration occurs in early stage after adult LDLT.There are many and different factors influencing graft regeneration at the different stages after LDLT.Graft volumetric measurement by using MSCT is a valid modality to evaluate graft regeneration after LDLT.