1.ANALYSIS OF TRANSCRIPTION OF PROTO-ONCOGENE C-myc, C-H-ras IN PATIENTS WITH ACUTE NONLYMPHOCYTIC LEUKEMIA
Fan ZHOU ; Yongxiang LIU ; Junjie CHU
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
RNA dot blot hybridization was employed to investigate the alterations in C-myc, C-H-ras transcript levels in bone marrow and peripheral blood cells from 24 patients with acute nonlymphocytic leukemia. 10 cases of normal bone marrow cells were used as control. We found that the positive rates and intensities of expression of C-myc and C-H-ras in acute nonlymphocytic leukemia were hipher than those of normal control. The C-myc and C-H-ras expressed simultaneously in 10 patients. After having been cultured 5 days with RA and low dose Ara-C combined with low dose Harr, the C-myc transcipt level of C-myc in acute nonlymphocytic leukemia was decreased obviously.
2.Stereotactic radiotherapy in the treatment of brain metastases
Yuanzhao LIU ; Hong FAN ; Defa CHU
Chinese Journal of Minimally Invasive Surgery 2001;0(02):-
4 ml, which was 45.1% (23 of 51)(?2=20.7, P4 ml were 3.8% (4 of 105) and 29.4% (15 of 51) respectively, with statistically significant difference between the two groups (?2=21, P68Gy was 81.3% (65 of 80), and group ≤68Gy 56.6% (43 of 76)(?2=11.1, P
3.Overview of traditional Chinese medical thesaurus and its relative thesaurus
Jing ZHANG ; Weiyu FAN ; Li PENG ; Jinong CHU
International Journal of Traditional Chinese Medicine 2011;33(5):436-439
An overview was performed on the features, structure, included terms, applications and the process of editing and compiling of traditional Chinese medical thesaurus and its relative thesaurus.
4.Near Infrared Spectroscopic Model Transfer Based on Simple Linear Regression
Huihua YANG ; Xiaofeng ZHANG ; Yongxian FAN ; Pumo XIE ; Xiaoli CHU
Chinese Journal of Analytical Chemistry 2014;(9):1229-1234
To solve the calibration transmission problem in near-infrared ( NIR) spectroscopy, a novel model transfer method, Simple Linear Regression Direct Standard-ization ( SLRDS ) , has been presented. To investigate the validity of the proposed method, a real corn sample NIR dataset was tested and the direct standardization ( DS ) method and piecewise direct standardization ( PDS ) method were involved as a comparison. Our results indicated that SLRDS can correct compressed NIR data differences among instruments and enable the user to share corn sample PLS calibration model among three instruments, at the same time it has higher prediction accuracy, fewer parameters and simpler model than DS and PDS.
5.Correlation Between Tei Index and Brain Natriuretic Peptide in Patients with Dilated Cardiomyopathy
Yajuan FAN ; Wen CHU ; Yajun LIANG ; Xuewei CHANG
Chinese Journal of Medical Imaging 2015;(6):449-452
Purpose To explore the relationship of Tei index and the concentration of plasma brain natriuretic peptide (BNP) in patients with dilated cardiomyopathy. Materials and Methods Fifty healthy individuals (control group) and fifty dilated cardiomyopathy patients (study group) were recruited in this study. BNP level was measured by enzyme-linked immunosorbent assay (ELISA). Color Doppler echocardiography were performed to measure the cardiac indices including left ventricular end-diastolic dimension (LVEDd), left ventricular end-systolic diameter (LVESd), interventricular septal thickness at diastole (IVSd), left ventricular ejection fraction (LVEF). Tei index was then calculated. The indices between the two groups were compared and correlation of Tei index and BNP with cardiac indices was analyzed. Results LVEDd, LVESd, Tei index, and BNP were significantly higher in study group than those in control group (t=14.4, 23.4, 21.9 and 22.3, P<0.01). IVSd and LVEF were significant lower in the study group (t=12.4 and 12.5, P<0.01). BNP was negatively correlated with LVEF (r= -0.266, P<0.05), and positively correlated with Tei index and LVEDd (r=0.401 and 0.326, P<0.05). Tei index was negatively correlated with LVEF and E/A (r= -0.480 and - 0.241, P<0.05), and positively correlated with LVEDd (r=0.375, P<0.05). Multiple stepwise regression analysis showed that the variates of BNP stepwise regression equation were Tei index, LVEF and LVEDd (t=5.984, -2.477 and 2.326, P<0.05); after correcting LVEF, the stepwise regression analysis showed closer relationship between Tei index and BNP (t=2.728, P<0.05). Conclusion There is correlation between Tei index and BNP. Tei index may be a good index for accurately estimating global ventricular function.
6.Mouse Embryonic Stem Cells Derived Feeder Cells Support the Growth of Themselves
ying-tang, SHI ; fan, TANG ; jian-xin, CHU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(05):-
Objective To test whether feeder cells derived from mouse embryonic stem cells(mESCs) could support the growth of mESCs themselves. Methods mESCs were induced to form mouse embryoid bodies(EB),and then fibroblast-like cells were derived from further differentiated mEB(mEB-dF),which served as feeder cells.The undifferentiation of mESCs grown on mEB-dF was confirmed by morphological analysis,colony efficiency and cell differentiation rate of mESCs,immunocytochemistry,alkaline phosphatase staining and RT-PCR.The pluripotency of mESCs grown on mEB-dF was examined by RT-PCR,inducing their differentiation in vivo and in vitro. Results(Forty-eight) fibroblast-like cells lines were derived from the same EB at three periods(d 10,d 15 and d 20),and five of them,mostly derived from d15 EB,were able to maintain mESCs in undifferentiated status and pluripotential ability over 10 passages.mESCs cultured on these feeder cell lines expressed alkaline phosphatase and specific mESCs markers,including SSEA-1,OCT-4,NANOG,and formed EB in vitro and teratomas in vivo.However,the majority of mEB-dF lines(43/48) has no such ability. Conclusion This study not only provides a novel feeder system for mESCs culture,avoiding lot of disadvantages of mouse embryo fibroblasts used as the feeder,but also indicates that fibroblast-like cells derived from mESCs take on different functions.The molecular mechanism of different function of these fibroblast cells is worthy of further investigations.
7.Internet and traditional Chinese medical thesaurus
Weiyu FAN ; Jing ZHANG ; Li PENG ; Jinong CHU
International Journal of Traditional Chinese Medicine 2010;32(4):343-344
This paper is intended to figure out how to develop and maintain the traditional Chinese medical (TCM)thesaurus in the Internet Era. The history of TCM thesaurus was reviewed. The function of TCM thesaurus in searching information in internet and the impact of internet to thesauri were discussed. Suggestions on descriptor/term selection, term relations, and thesaurus maintaining were put forward.
8.An experiment control study on the ovarian reserve function after cisplatin intraperitoneal or intravenous chemotherapy in rats model
Bozhen FAN ; Hong XIA ; Lei CHU ; Xiaowen TONG
Chinese Journal of Obstetrics and Gynecology 2017;52(4):249-253
Objective To compare the impact on the ovarian reserve function after cisplatin intraperitoneal or intravenous chemotherapy in rats model. Methods Thirty 8-weeks old female Sprague Dawley rats were randomly assigned to control group (group A, n=10), intraperitoneal chemotherapy group (group B, n=10) and intravenous chemotherapy group (group C, n=10). Cisplatin was diluted by normal saline (NS) into 4 mg/ml. On the first day of chemotherapy, 0.2 ml cisplatin dilution was injected into the abdomen of rats in group B, isodose cisplatin was injected into vein and 1.8 ml NS was injected into abdomen of rats in group C, 2.0 ml NS was injected into abdomen of rats in group A for control. Feed the three groups rats and test the anti-Mullerian hormone (AMH) in serum on day 0 (just before injection), day 10 and day 20 by ELISA, count the numble of follicle in bilateral ovaries on day 20. Results (1) The levels of serum AMH in the three groups before and after chemotherapy were compared: ① comparison between groups: On day 10 and day 20 , the AMH level in group B [(64.5 ± 2.9), (68.6 ± 3.4) ng/L] and group C [(76.1±4.9), (91.3±3.9) ng/L] was significantly lower than that in group A [(120.1±5.3), (121.7±4.6) ng/L;P<0.01], AMH level in group B was significantly also lower than that in group C (P=0.000). ② Comparison within groups:the AMH level on day 0 was significantly lower than that on day 10 and day 20 in group A (P<0.01), but there was no significant difference between day 10 and day 20 (P=0.427). The AMH level on day 0 was significantly higher than those on day 10 and day 20 in group B (P<0.01) and group C (P<0.01). There was no difference in AMH level between day 10 and day 20 (P=0.124) in group B, but the level was significant lower on day 10 than that on day 20 in group C (P=0.011). (2)Comparison of the number of follicles in ovaries of three groups 20 days after chemotherapy:the follicles number in group A(35±13)was greater than that in group B (16 ± 9,P=0.003) and similar with group C (31 ± 16,P=0.474) on day 20. The follicles number in group B was significantly less than that in group C (P=0.018). Conclusions In the present study, both intravenous and intraperitoneal chemotherapy have impacts on ovarian reserve function and the latter might be more serious. The level of AMH will rise again over time after chemotherapy and rats undergo intravenous chemotherapy would recover faster. The results suggest that the ovarian reserve function need more time to recover after intraperitoneal chemotherapy. For patients with ovarian cancer who want to preserve fertility function, intravenous chemotherapy might be more appropriate.
9.Management of medical subject headings
Li PENG ; Jinong CHU ; Weiyu FAN ; Jing ZHANG
International Journal of Traditional Chinese Medicine 2013;35(12):1107-1109
Medical subject headings (MeSH) was compiled by American state-run medical library.In this article,the update maintenance,data publication,technological support,and management of human and finical resources on MeSH were introduced.
10.Renovation principle of medical subject headings
Jing ZHANG ; Li PNEG ; Weiyu FAN ; Jinong CHU
International Journal of Traditional Chinese Medicine 2013;35(8):716-718
In this article,the purpose,period,overview,and principle of medical subject headings renovation was introduced,to provide references for national headings renovation and maintenance.