<p>OBJECTIVESTo study the expression patterns of two Eph family molecules, the receptor EphA5, and the ligand ephrin-A5, during spinal cord development.p><p>METHODSThe receptor expression was analyzed using beta-galactosidase knockin mice, and affinity ligand probe binding. The ligand expression was assessed using two different affinity probes, and knockout mouse tissues as controls.p><p>RESULTSEphA5 was expressed in the ventral spinal cord, while ephrin-A5 was located in the dorsolateral regions of the spinal cord throughout development.p><p>CONCLUSIONSThese results show that EphA5 and ephrin-A5 are expressed over broad developmental stages and may play important roles in establishing the dorsoventral organization of the spinal cord.p>
Animals ; Ephrin-A5 ; biosynthesis ; Gene Expression ; Gene Expression Regulation, Developmental ; Immunohistochemistry ; Mice ; Mice, Inbred C57BL ; Mice, Mutant Strains ; Receptor, EphA5 ; biosynthesis ; Spinal Cord ; embryology ; metabolism