Separation of Langerhans cells in epidermis of 16 healthy Korean individuals were performcd. Separation of Langerhans cells by density gradient centrifugation on a colloidal sillica(percoll) polyvinilpyrrolidone gradient. And autologous, allogeneic mixed skin cell leukocyte culture reaction was done with each fractionatcd cpidermal cell suspensions. Also lymphocytes, epidermal cells was cultured in media alone, respectively. The results was quantitated by the incorporation of H-thymidine by p-liquid scintillation counter. The densities of I angerhans cells within the epidermal cells, fraction-2 was most higher concentration (22.0+2.8%) and fraction-5 was most lower concentration (3.4+ l.9%). 2. In the comparison of the results of Langehans cells enriched and depleted population in autologous mixed skin cell leukocyte culture reaction, the former was higher than the latter on lymphocyte stimulatory capacity. There was significant differences(p<0.005) And also same as result in allogeneic mixed skin cell leukocyte culture reaction. 3. Langerhans cells enriched fraction in this study was more lymphocyte stimulatory capacity than depleted fraction in allogeneic mixed skin cell leukocyte culture(p<0.01~0.05). Ailogeneic mixed skin cell leukocyte culture reaction was more lymphocyte stimulatory capacity than the autologous(p<0.005~0.05).
Centrifugation, Density Gradient* ; Colloids* ; Epidermis ; Humans* ; Langerhans Cells* ; Leukocytes* ; Lymphocytes ; Scintillation Counting ; Silicon Dioxide* ; Skin* ; Suspensions

Nevocellular nevi are benign tumors composed of nevus cells, which are peculiar to man and almost everyone has a few nevi somewhere on his body. The dermatologist has a unique opportunity to study the most common neoplaam of man, the nevocellular nevus, becasue of its ready accessibility to visual observation and its simplicity obtaining for microscopic specimen. Much has been leamed last two decades about the nature, origin, and development of the nevocellular nevus. However, most of the these knowledge obtained by investigation of Caucasians. The investigation of colored subjects are scarcely reported. This paper is an attempt to clarify 1) the clinical features of nevocellular nevi developed on Korean, colored race. 2) the incidence of pigmented nevi developed on normal individuals and vitiligo patients. During the last l0 month(1975. 3. - 1975. 12. ) 525 normal individuals and 67 vitiligo patients were randomly selected and were investigated about incidence and clinical morphology(according to Levers clinical classification) of pigmented nevi developed on different body areas. The results were summarized as follows. 1) There was average 20 nevocellular nevi in each Korean individuals. Average 23 nevi was found in male and 15 nevi in female. 2) The number of nevus and its morphology were closely related to the age of host and body areas. 3) The flat type nevi was the most common type nevi and face was the most common site among the body areas. 4) In comparison with Caucasian, it seemed no racial difference in clinical features of the pigmented nevus. 5) There was no difference between the incidence of nevocellular nevus developed on normal individual and that of vitiligo patient.
Continental Population Groups ; Female ; Humans ; Incidence ; Korea* ; Male ; Nevus* ; Nevus, Pigmented ; Vitiligo

We report a case of pretibial myxedema with hypothyroidism in a 52-year-old woman. She suffered from primary hypothyroidism for a period of 17 years. She was treated with a daily dose of sodium levothyroxine 0.2 mg intermittently. She had erythematous ftesh colored, hard and thickened plaque. on the left pretibial area for onr year. The histopathologic findings showed considerable amount of muci in the upper and mid dermis. Toluidine blue and alcian blue stain demonstrated an extensive deposition of dermal mucin. We treated her with a topical steroid and oral sodium levothyrcxire.
Alcian Blue ; Dermis ; Female ; Humans ; Hypothyroidism* ; Middle Aged ; Mucins ; Myxedema* ; Sodium ; Thyroxine ; Tolonium Chloride

We report a case of cutaneous focal mucinosis in a 60 year-old male, who had asymptomatic localized small flesh colored papule and nodule on the left side of the neck. The histopathologic finding revealed fibroblastic proliferatior in the dermis and an increased prodact on of hyaluronic acid by these fibroblasts. The lesions were treated by surgical excision.
Dermis ; Fibroblasts ; Humans ; Hyaluronic Acid ; Male ; Middle Aged ; Mucinoses* ; Neck

Ae opposed to most other drug eruptions, drug-induced pemphigus may not develop for at least several months following the initiation of therapy with the offending agent. Unlike sporadically occuring pemphigus, the clinical and hietopathologic patterns of drug-induced pemphigus are most often those of pemphigus foliaceus. We report a case of diphenylhydantoin-induced pemphigus occuring in 30-year-old male with epilepsy. A few coinsized erythematous scaly patches developed on his fae, chest, snd left wrist, and two flaccid bullae on erythemstous base developed on his abdomen after three years snd nine months of therapy with diphenylhydantcin. Histopathologicslly, a biopsy specimin taken from a bullous lesion revealed that bulla is formed just beneath the granular layer and aeaitholytic cells are seen within the bulla. Direct and indirect irnmunofluorescence studies showed regitive findings. Administration of diphenylhydantoin was discontinued and he was treated with systemi and topical corticoeteroid. After 4 weeks, the skin lesions were healed with postinflammatory hysroigmentation and no new lesions were developed thereafter.
Abdomen ; Adult ; Biopsy ; Blister ; Drug Eruptions ; Epilepsy ; Humans ; Male ; Pemphigus* ; Phenytoin* ; Skin ; Thorax ; Wrist

Phakomatosis pigmentovascularis was first reported in 1947 as adiease complex with a distinct association of pigmentary nevus such as nevus of Ota, nevus of Ito, bilon golian spots, nevus spilus and cutaneous hemangioma such as nevus flammeus, which wss assoeisted with systemic organ involvment, We report a case of phakomatosis pigmentovascularis in a 11-year-old girl who had nevus flammeus, nevus spilus, and n.evus of Ota over various parts of her bo by since birth. She was also associated with leg-length discrepancy, but other systemic involvement was absent,
Child ; Female ; Hemangioma ; Humans ; Leg* ; Neurocutaneous Syndromes* ; Nevus ; Nevus of Ota ; Parturition ; Port-Wine Stain

BACKGROUND: Livedo vasculitis shows variable clinical and histopathologic features according to its courses. There are also some discrepancies in histopathologic findings between the authors. OBJECTIVE: Our purpose is to clarify the clinical and istopathologic feat tures of livedo vasculitis. METHODS: We reviewed the clinical and histopathologic features in cluding direct immunofluorescence study and response to treigtment in eight patients with livedo vasalitis. RESULTS: The ratio of males to females was 1:3 with female predusm nance. Age of onset was predominant, in young to middle-age, ranging from 19 to 64 years old. In seven patients skin lesions were aggravated during the summe, and in one patient during the winer. All patients had purpuric or telangiectatic lesions on the lower extremities. Painful ulcers developed in seven patients and some of the ulcers healed with whitish atrophic scars. Five patients had preceeding livedo reticularis. One patient had Raynauds phenomenoin and the other patients did not show alinormal findings related to the systemic diseases. Histopathologically, in two patients fibrinoid materi.il was deposited in the vessel walls mildly, and in six patients obviously with partial to complete obst,r iction of blood vessels. Extravasation of RBCs and thrombus formation were found clearly in seven patients, but in one patient these findings were observed in a mild degree. Endothelial swellings were obserrved in all patients. Perivascular infiltrations of mononuclear cells were also observed in all patients in various degrees. But only two patients with secondary bacterial infection showed neutrophil infilt ations or nuclear dusts. So, histopathologic findings are corripatible with lymphocytic vasculitis. The locations of predominantly affected vessels were variable. Direct immunofluorescence studies were done in six patients. Four of these patients showed positive findings. At least 4 months were needed for the complete healing of the skin lesions. CONCLUSION: Livedo vasculitis is chronic vasculit is aggravated in mostly summer. Most of the initial purpuric lesions progress to ulcers which is healed with whitish atrophic scars. Histopathoiogic findings suggest livedo vasculitis is a sort of lymphocytic vasculitis.
Age of Onset ; Bacterial Infections ; Blood Vessels ; Cicatrix ; Dust ; Female ; Fluorescent Antibody Technique, Direct ; Humans ; Livedo Reticularis ; Lower Extremity ; Male ; Middle Aged ; Neutrophils ; Skin ; Thrombosis ; Ulcer ; Vasculitis*

No abstract available.
Adult* ; Humans ; Myofibroma*

Hypohidrotic ectodermali dysplasia is a rare and heterogenous genodermatosis that is characterzed by hypohidrosis, hyptrichosis, dental hypoplasia, and a characteristic facies. A 16-month-old male was evaluated for anodontia and anhidrosis since birth. There was no family history of this disease. His face was characterized by a broad nose and sparse eyebrows and periorbital wrirkling. The skin was dry, and scalp hairs were thin and sparse. Skull X-ray finding revealed noth ing remarkable except de;ayed teeth eruption. His developmental status for which we used DDS'I' was slightly reduced on inental development and showed normal on physical development. A skin biopsy was done on skin from the left palm, which showed a few immature eccrine glands in the dermis. We report a typical form of hypohidrotic ectodermal dysplasia with a review of the literature.
Anodontia ; Biopsy ; Dermis ; Eccrine Glands ; Ectodermal Dysplasia 1, Anhidrotic* ; Eyebrows ; Facies ; Hair ; Humans ; Hypohidrosis ; Infant ; Male ; Nose ; Parturition ; Scalp ; Skin ; Skull ; Tooth

There are three major type of malignant melanoma which differ in their mode of onset, course; lentigo maligna melanoma, superficial spreading malignant melanoma and nodular malignant melanoma. Malignant melanoma may aris on apparently normal skin, in a junction nevus, rarely in a compound nevus and extremely rately in an intradermal nevus. We presented a case of malignant melanoma arising from compoud nevus in 51 year old male patient. Ulcerated verrucous walnut sized brown-black tumor mass was nticed on the posterolateral surface of right foot. There were no palpable lymph nodes in whole body. Tumor cells were epithelioid type and lie in irregularly alveolar formation histologically. There were also numerous nevus cells in dermis and nevus cell nest in lower epidermis with tumor cells. Surgical excision of tumor mass and skin graft was performed.
Dermis ; Epidermis ; Foot ; Humans ; Hutchinson's Melanotic Freckle ; Juglans ; Lymph Nodes ; Male ; Melanoma ; Middle Aged ; Nevus* ; Nevus, Intradermal ; Skin ; Transplants ; Ulcer