Separation of Langerhans cells in epidermis of 16 healthy Korean individuals were performcd. Separation of Langerhans cells by density gradient centrifugation on a colloidal sillica(percoll) polyvinilpyrrolidone gradient. And autologous, allogeneic mixed skin cell leukocyte culture reaction was done with each fractionatcd cpidermal cell suspensions. Also lymphocytes, epidermal cells was cultured in media alone, respectively. The results was quantitated by the incorporation of H-thymidine by p-liquid scintillation counter. The densities of I angerhans cells within the epidermal cells, fraction-2 was most higher concentration (22.0+2.8%) and fraction-5 was most lower concentration (3.4+ l.9%). 2. In the comparison of the results of Langehans cells enriched and depleted population in autologous mixed skin cell leukocyte culture reaction, the former was higher than the latter on lymphocyte stimulatory capacity. There was significant differences(p<0.005) And also same as result in allogeneic mixed skin cell leukocyte culture reaction. 3. Langerhans cells enriched fraction in this study was more lymphocyte stimulatory capacity than depleted fraction in allogeneic mixed skin cell leukocyte culture(p<0.01~0.05). Ailogeneic mixed skin cell leukocyte culture reaction was more lymphocyte stimulatory capacity than the autologous(p<0.005~0.05).
Centrifugation, Density Gradient* ; Colloids* ; Epidermis ; Humans* ; Langerhans Cells* ; Leukocytes* ; Lymphocytes ; Scintillation Counting ; Silicon Dioxide* ; Skin* ; Suspensions

Nevocellular nevi are benign tumors composed of nevus cells, which are peculiar to man and almost everyone has a few nevi somewhere on his body. The dermatologist has a unique opportunity to study the most common neoplaam of man, the nevocellular nevus, becasue of its ready accessibility to visual observation and its simplicity obtaining for microscopic specimen. Much has been leamed last two decades about the nature, origin, and development of the nevocellular nevus. However, most of the these knowledge obtained by investigation of Caucasians. The investigation of colored subjects are scarcely reported. This paper is an attempt to clarify 1) the clinical features of nevocellular nevi developed on Korean, colored race. 2) the incidence of pigmented nevi developed on normal individuals and vitiligo patients. During the last l0 month(1975. 3. - 1975. 12. ) 525 normal individuals and 67 vitiligo patients were randomly selected and were investigated about incidence and clinical morphology(according to Levers clinical classification) of pigmented nevi developed on different body areas. The results were summarized as follows. 1) There was average 20 nevocellular nevi in each Korean individuals. Average 23 nevi was found in male and 15 nevi in female. 2) The number of nevus and its morphology were closely related to the age of host and body areas. 3) The flat type nevi was the most common type nevi and face was the most common site among the body areas. 4) In comparison with Caucasian, it seemed no racial difference in clinical features of the pigmented nevus. 5) There was no difference between the incidence of nevocellular nevus developed on normal individual and that of vitiligo patient.
Continental Population Groups ; Female ; Humans ; Incidence ; Korea* ; Male ; Nevus* ; Nevus, Pigmented ; Vitiligo

Trichorhinophalangeal syndrome (TRPS) was first described in 1966 by Giedion. It is a rare genetic disease and divided into two groups : TRPS type I and TRPS type II. TRPS type I is an autosomal dominant inherited disorder, defined by three characteristic findings : first, sparsely distributed brittle and slow-growing scalp hair, second, a bulbous pear-shaped nose with an elongated philtrum, third brachydactyla with angular deformities. The characteristic radiological features of the hands include cone shaped epiphysis of the proximal interphalangeal joints. Most TRPS type II is sporadic and shows multiple exosteosis, mental retardation and microcephaly in addition to the three chracteristic findings of TRPS type I . A 20-year-old female visited our department for the evaluation of her sparse, slow-growing scalp hair. She also had a bulbous pearshaped nose, ulnar deviated fingers and shortened both hallux. Some relatives of patients show similar symptoms of scalp hair. A Radiologic aly, histopathologic aly and chromosomal study were done, and we established the diagnosis, TRPS type I.
Congenital Abnormalities ; Diagnosis ; Epiphyses ; Female ; Fingers ; Hair ; Hallux ; Hand ; Humans ; Intellectual Disability ; Joints ; Lip ; Microcephaly ; Nose ; Scalp ; Young Adult

Bleomycin is an antitumoral antibiotic derived from Streptomyces verticillus in 1965. The drug has been used with a varying success in the treatment of squamous cell carcinomas and malignant lymphomas. The cutaneous toxicities of bleomycin include hyperpigmentation, sclerosis, gangrene, nail changes and erythema. Linear streaks appear to be a characteristic eruption induced by bleomycin. We report three cases of flagellate pigmentation from intravenous bleomycin. Three patients were suffering from uterine cervical carcinomas and were treated with chemotherapy with a VBP (vincristine, bleomycin, cisplatire) regimen. During or after their courses of chemotherapy, dark brownish skin lesions developed on the body surface of these patients. A Light microscopic study of each specimen showed increased pigmentation of the basal layer and an electron microscopic study showed a slightly increased number of melanosomes in keratinocytes.
Bleomycin* ; Carcinoma, Squamous Cell ; Drug Therapy ; Erythema ; Gangrene ; Humans ; Hyperpigmentation ; Keratinocytes ; Lymphoma ; Melanosomes ; Pigmentation* ; Sclerosis ; Skin ; Streptomyces

In 1968, Hardy and Anderson first described the term hypereosinophilic syndrome. This syndrome is characterized by persistent and prolonged eosinothila, with primarily hematologic, cardiac, neurologic and derrnatologic abnormalities. Cutaneous mmestations occur in 27-57% of patients. Two types of skin lesions have been noted: (1) erythrritous pruritic papules and nodules or (2) urticaria and angioedema. We report a case of hypereosinophilic syndrome with a vesicle eruption which is a rare skin lesion in tbis syndrome, in a 17 year old man. Diagnosis of hyprcsinophilic syndrome was established by clinical findings, rearked blood eosinophilia without a Brown cause, bone marrow aspiration and biopsy, liver scan and ultrasonography, and histopatholcgie findings of the skin.
Adolescent ; Angioedema ; Biopsy ; Bone Marrow ; Diagnosis ; Eosinophilia ; Humans ; Hypereosinophilic Syndrome* ; Liver ; Skin ; Ultrasonography ; Urticaria

Pityriasis Rubra Pilaris is a rare, chronic, mildly inflammatory disease characterized by fine acuminate follicular papules with orange-red to salmon-colored scaling of the skin and erythroderma that surround islands of normal, uninvolved skin, particular in the trunk. Most patients also develop palmoplantar hyperkeratosis in the early course of the disease. Focal acantholysis occurring in pityriasis rubra pilaris is an unusual and incidental histologic finding within the spectrum of histologic change of the disease. No case featuring this concomitant histologic finding has been reported in the Korean literatures up to date. We report a case of focal acantholysis occurring in a patient with the clinical features of pityriasis rubra pilaris which may be considered as an incidental finding.
Acantholysis* ; Dermatitis, Exfoliative ; Humans ; Incidental Findings ; Islands ; Pityriasis Rubra Pilaris* ; Pityriasis* ; Skin

The role of the immune system in pathogenesis of skin cancer has been attracted increasing attention in recent years. Some limited studies and observations suggest that altered immune status may be a factor in the initiation and growth of cutaneous malignancies. Authors experienced a typical case of xeroderma pigmentosum in a 6 year-old girl, who has been suffered from hyper and hypopigmented macules on sun-exposed area, photophobia and also noted eroded lesion on the lower lip. The biopsy finding of the hyperpigmented macule showed that of freckle and the finding of actinic cheilitis was observed from the specimen of eroded 1esion on the lower lip. We performed immunologic test and obtained some evidences of immunologic defect; such as decreased numbers of total T cells, T-suppressor cell and B cell in our patient.
Actins ; Biopsy ; Cheilitis ; Child ; Cytochrome P-450 CYP1A1 ; Female ; Humans ; Ichthyosis* ; Immune System ; Immunologic Tests ; Lip ; Melanosis ; Photophobia ; Skin Neoplasms ; T-Lymphocytes ; Xeroderma Pigmentosum*

Carcinomas of apocrine glands are rare and their diagnostic features are poorly defined. Only about 35 cases were reported in the literatures from 1911 to 19871. Their occurrence has been reported mainly in the axilla and breast area. We present a case of apocrine carcinoma arising from the left axilla. The literature is reviewed with respect to clinical and histopathologic findings.
Apocrine Glands* ; Axilla ; Breast

BACKGROUND: The visible cutaneous pigmentary response to ultraviolet A(UVA) is immediate, whereas ultraviolet B(UVB)-induced pigmentation appears after a delay of several days. However, some investigators reported that UVA also can induce delayed taniing. OBJECTIVE: In order to determine whether the pigmentation induceil by UVA irradiation is accompanied by melanocytes responses which are the same or different from those following a UVB-indueed tan, we irraiated malignant melanoma cells to UVA or UVB. METHOD: On the 7th day after irradiation of UVA or UVB, we exa nined the cell counts and the melanin content of control and experimental groups. RESULTS: Although a dose of 10 J/cm decreased the number of manignant melanoma cells, there was no significant difference between the control and UVA-exposure griiups. But there was a significant decrease after UVB-exposun. UVB-exposure groups showed a significant increase of melanin cortent. A dose of 10 J/cm of UVA also induced a significant increase of melanin content. CONCLUSIONS: The data suggest that UVA at a clinically relevant dose has a significant effect on human melanocytes. So, UVA very likely plays a role in the increased, melanization associated with delayed tanning.
Cell Count ; Cell Line* ; Humans ; Melanins ; Melanocytes ; Melanoma* ; Pigmentation ; Research Personnel ; Tanning ; Triacetoneamine-N-Oxyl