1.Effect of electrical stimulation for bony fixation of the porous coated intramedullary stem.
Young Min KIM ; Choon Ki LEE ; Hee Joong KIM ; Yong Min KIM ; Sung Soo CHUNG
The Journal of the Korean Orthopaedic Association 1992;27(1):318-326
No abstract available.
Electric Stimulation*
2.Cystic lesion with visible feeding vessel in chest PA.
Chul Hyun KIM ; Soo Taek UH ; Yeon Tae CHUNG ; Yong Hoon KIM ; Choon Sik PARK
Tuberculosis and Respiratory Diseases 1991;38(1):83-87
No abstract available.
Thorax*
3.A Case of Polygrandular Automune type II syndrome associated empty sella.
Jin Soo KIM ; Mi Duk LEE ; Hong Seung KIM ; Choon Hee CHUNG
Journal of Korean Society of Endocrinology 1998;13(2):295-300
The polyglandular autoimmune(PGA) syndrome designate as the dysfimction of endocrine and nonendocrine systems involving two or more organs on the basis of an autoimmune mechanism. There are 3 types of PGA syndrome and their etiology or pathogenesis is still not complete by understood. Type I PGA is present in the patients who have at least two of the triad of Addison's disease, hypopacathyroidism, and chronic mucocutaneous candidiasis. Type II PGA is present in the those who have Addisons disease with autoimmune thyroid disease and/or insulin dependent diabetes mellitus, but who do not have hypoparathyroidism or candidiasis. Type III PGA is present in the one who have autoimmune thyroid disease, without Addisons disease, but with another autoimmune disease report a case of autoimmune polyglandular syndrome type II in a seventy-three years old female patient who had primary adrenal insufficiency, primary hypothyroidism, and empty sella, pulmonary tuberculosis.
Addison Disease
;
Autoimmune Diseases
;
Candidiasis
;
Candidiasis, Chronic Mucocutaneous
;
Diabetes Mellitus
;
Female
;
Humans
;
Hypoparathyroidism
;
Hypothyroidism
;
Insulin
;
Polyendocrinopathies, Autoimmune
;
Thyroid Diseases
;
Tuberculosis, Pulmonary
4.The role of NK cell in heart-lung transplanted mice.
Duck Jong HAN ; Kun Choon PARK ; In Koo KIM ; Dae Won KIM ; Kyung Sook CHUNG
The Journal of the Korean Society for Transplantation 1991;5(1):143-149
No abstract available.
Animals
;
Killer Cells, Natural*
;
Mice*
5.Estrogen receptor proteins in gastrointestinal adenocarcinoma.
Jin Cheon KIM ; Byung Sik KIM ; Kun Choon PARK ; Myung LEE ; Yoon Young CHUNG ; In Chul LEE
Journal of the Korean Surgical Society 1992;42(4):471-476
No abstract available.
Adenocarcinoma*
;
Estrogens*
6.Dynamic Salivary Gland Scintigraphy in Clinical Sicca Syndrome: Comparison with Static images.
Euy Neyng KIM ; Hyung Sun SOHN ; Jung Eun CHOI ; Sung Hoon KIM ; Yong An CHUNG ; Soo Kyo CHUNG ; Choon Yul KIM
Korean Journal of Nuclear Medicine 2001;35(1):43-51
PURPOSE: In this study, we compared the quantitative characteristics of dynamic salivary gland scintigraphy with static scintigraphy in patients with clinical sicca syndrome using Tc-99m pertechnetate. MATERIALS AND METHODS: Fifty-two parotid glands and 52 submandibular glands out of 26 patients with clinical sicca syndrome were studied by dynamic and static salivary gland scintigraphy. Ten normal volunteers were also studied as a control group for comparison of scintigraphic parameters. Ten minutes after injection of 370 MBq Tc-99m pertechnetate, we obtained pre-stimulus static images for a few minutes. Then dynamic salivary gland scintigraphy with lemon juice stimulation was performed for 20 minutes. Finally we obtained post-stimulus static images after dynamic images. On dynamic study, functional parameters such as uptake rate, secretion rate and re-uptake rate were calculated. The results of dynamic study and static images were compared. RESULTS: On dynamic study, we could obtain functional parameters of salivary glands successfully. On dynamic study, 22 parotid glands and 22 submandibular glands out of each of 52 glands are abnormal. The static images demonstrated somewhat different results, of which reasons we could assume via dynamic study. CONCLUSION: Dynamic salivary gland scintigraphy using Tc-99m perechnetate were more functional than static images and might be useful in the assessment of the functional change of the salivary gland in patients with clinical sicca syndrome.
Healthy Volunteers
;
Humans
;
Parotid Gland
;
Radionuclide Imaging*
;
Salivary Glands*
;
Sjogren's Syndrome*
;
Sodium Pertechnetate Tc 99m
;
Submandibular Gland
7.Magnetic Resonance Imaging Findings of Intraspinal Neurenteric Cyst: Case Report.
Dong Ik KIM ; Choon Sik YOON ; Pyeong Ho YOON ; Tae Sub CHUNG ; Sang Wook YOON
Journal of the Korean Radiological Society 1994;31(4):621-625
Intraspinal neurenteric cysts are rare congenital lesions that results from abnormal separation of germ layers in the third week of embryonic development, which may cause spinal compression. Although, the diagnosis of neurenteric cyst was very difficult prior to operation, MRI has proven to be a useful imaging modality in detection, localization and characterization of intraspinal neurenteric cysts. We recently experienced intraspinal neurenteric cyst in two patients who presented with progerssive quadriparesis. Myelography, CT myelography and MRI were taken and complete excision was performed. The MRI findings are presented and the literature is reviewed.
Diagnosis
;
Embryonic Development
;
Female
;
Germ Layers
;
Humans
;
Magnetic Resonance Imaging*
;
Myelography
;
Neural Tube Defects*
;
Pregnancy
;
Quadriplegia
8.The Differentiation of HL-60 Cells Causes to Lose their Ability to Express TNF mRNA.
Jung Hyun KIM ; Soo Jin HAN ; Choon Sik PARK ; Il Yup CHUNG
Korean Journal of Immunology 1999;21(1):47-53
It is well known that HL-60 cell, a human promyelocytic line, is differentiated into eosinophil-like cells in the presence of butyric acid, and thus the differentiated HL-60 cells have been used as a model system to study irnmunological properties of peripheral eosinophils which are thought to be terminally differentiated. To study whether HL-60 cells alter their capability of expressing cytokines during differentiation to eosinophil-like cells, we examined TNF mRNA levels in HL-60 cells treated with butyric acid by Ribonuclease Protection Assay (RPA). HL-60 cells were incubated for 3 days in the presence of butyric acid (0.5 mM), and stimulated with PMA and lipopolysaccharide (LPS). The levels of TNF mRNA decreased by 50 % and 95 % upon one and two days of post-treatment of butyric acid, respectively. The decreased pattern in TNF mRNA levels was also observed in HL-60 cells that have been treated with retinoic acid known as an inducer for differentiation of them. In accordance with these results, prominent azurophilic granules typical in eosinophils appeared in the cytoplasm of the differentiated HL-60 cells. The decreased expression of TNF mRNA was not attributable to the presence of serum, since increasing concentrations of serum had no effect. Furthermore, interleukin-5 (IL-5), which is known to be involved in activation and trafficking of eosinophils in vivo and in vitro, failed to affect TNF mRNA production when it was used in place of butyric acid. These data suggest that the differentiated HL-60 cells may have immunological resemblance to eosinophils in that they weakly produce the cytokine mRNA.
Butyric Acid
;
Cytokines
;
Cytoplasm
;
Eosinophils
;
HL-60 Cells*
;
Humans
;
Interleukin-5
;
Ribonucleases
;
RNA, Messenger*
;
Tretinoin
9.A study on the usefulness of HbA1c for diagnosis in patients withdiabetes mellitus.
Young Sik CHOI ; Young Ho YOON ; Wha Soon CHUNG ; Tae Yeal CHOI ; Choon Won KIM
Korean Journal of Clinical Pathology 1991;11(1):31-39
No abstract available.
Diagnosis*
;
Humans
10.Adenovirus - Mediated gene Transfer of Wild - Type p53 Results in Restoration of Tumor - Suppressor Function in Glioma Cell Lines.
Mi Sook KIM ; Hee Chung KWON ; Seok Il HONG ; Choon Taek LEE ; Seung Hoon LEE
Journal of the Korean Cancer Association 1998;30(5):1026-1033
PURPOSE: The replacement of functional genes into cells that lack genes or mutant genes is the basis of gene therapy. In cancer, where cells often have multiple genetic defects, the replacement of critical genes may suffice to suppress cell growth or induce cell death. In malignant brain tumors, p53 mutation are among the most frequently observed genetic findings and inactivation p53 suggests that p53 plays a critical role in carcinogenesis and tumor progression. Therefore, we study the successful transfer of the wild-type p53 gene using a replicative deficient adenovirus vector into human glioma and medulloblastoma c~ell lines. Meterials and Methods: The human glioma cell line T-98G, U-87MG, U-373MG were used. To determine the efficiency of the adenovirus vector, cell lines were transfected with the Ad-p gal and analysed with X-Gal staining. Cell viability was determined by trypan blue exclusion every day after infection and Westem blot analysis was used to conform the expression of the exogenous p53 protein. RESULTS: Cell growth of the Ad-CMV-p53 infected U-373MG, and U-87MG was significantly suppressed. It appeared that exogenous p53 protein expression had an earlier ad more profound suppressive effect on U-373MG having a mutated p53 gene than on U-87MG having a wild-type p53. The expression of the exogenous p53 was more than 10 times higher than the expression of the endogenous p53. To examine the decreased viability, U-373MG was stained with Hochest 33258 and detected nuclear condensation and apoptic body. Staining results suggest that cells undergo apoptosis. CONCLUSION: The replicative deficient adenoviral vector can transfer and express p53 in human glioma cell lines in vitro, restoring wild-type p53 tumor suppressor functions. The restoration of normal p53-encoded protein in the mutant ceil lines induced cell death. The high expression of the newly transduced protein had different effects on the growth rate of the infected cell lines depending on the p53 status of the cells.
Adenoviridae*
;
Apoptosis
;
Brain Neoplasms
;
Carcinogenesis
;
Cell Death
;
Cell Line*
;
Cell Survival
;
Genes, p53
;
Genetic Therapy
;
Glioma*
;
Humans
;
Medulloblastoma
;
Trypan Blue