This paper presents the result and statistical analysis of the experiment, in which selective anterior or posterior rhizotomy were carried out under microscopic surgery. The materials used for the experiment were 48 young rabbits, and they were divided into four groups. The group I, which was used as control, was subjected to lower dorsal bilateral laminectomies with dural incision, the group II to laminectomies with three right posterior rhizotomies, the group III to laminectomies with three right anterior rhizotomies, and the group IV to laminectomies with three right anterior and posterior rhizotomies. Scoliosis was induced in group Il, Ill and IV. There was no significant statistical difference in the degrees of curvatures among these three groups. The convexity of the curvature faced to the side of the divided roots. In group III and IV, scoliotic curvatures were obvious within two weeks, but there were no considerable changes thereafter. In group Il, the curves progressed slowly up to the 12th week. They were statistically significant at the 4th week and increased continuously thereafter. The histological findings in the group III and IV showed denervation atrophy of the paravertebral muscles at the apex of the curvature, but there was little or no muscle atrophy in the group I and II. We came to a conclusion from the experimental results that scoliosis may be induced not only by anterior root paralysis but also by selective posterior root paralysis.
Atrophy ; Denervation ; Laminectomy ; Muscles ; Muscular Atrophy ; Paralysis ; Rabbits ; Rhizotomy ; Scoliosis

A clinical observation was made on 5 cases of primary ureteral tumors admitted to the Department of Urology, Presbyterian Medical Center during the period from August, 1970 through March, 1978. Clinical and radiographic findings are summarized below: 1. Among 5 cases of primary ureteral tumors, 4 cases in male, 1 case in female. 2. The range in age was from 58 to 75 years, average 66 years. 3. Duration of symptoms varied from 1 day to 1 year, common symptoms are hematuria and flank pain (1 case). 4. The side of lesion: left 3 cases , right 2 cases. 5. All tumors are located in the lower third of ureter. 6. The characteristic urographic and cystoscopic findings are as follows ; Non-visualizing kidney disclosed by intravenous pyelogram in 3 cases, hydroureteronephrosis in 2 cases. filling defect of ureter in 2 cases. The appearance of tumor in the orifice on cystoscopy in 2 cases. 7. Nephroureterectomy with excision of a cuff of the bladder was performed in all cases.
Cystoscopy ; Female ; Flank Pain ; Hematuria ; Humans ; Kidney ; Male ; Protestantism ; Ureter* ; Urinary Bladder ; Urology

Limb lengthening is now an accepted form of correcting limb length inequality. It is, however, associated not infrequently with a variety of complications including pheripheral nerve palsies. Such nerve dysfunction is obviously dependent on the velocity and amount of bone lengtening. In this experiment, the author attempted to determine the safe limits of the velocity and amount of bone lengthening by measuring somatosensory evoked potential (SSEP). The tibiae of 96 rabbits, weighing from 1.5 to 1.6kg, were osteotomized; in Group I, the tibiae were lengthened 0.35mm per day once daily;in Group II, 0.7mm per day; in Group III, 1.05mm per day; and in Group IV, 1.4mm per day. Several rabbits were subjected to the study weekly until, six weeks postoperatively in Group I, five weeks postoperatively in Grup II, four weeks postoperatively in Group III, and three weeks postoperatively in Group IV. The following results were obtained: 1. Preliminarily, in order to verify the possible difference between the right and left tibiae, Pl latency and amplitude were measured in 18 rabbits. Paired t-test revealed no statistically significant difference between the two (p>0.05). The amplitude manifested more individual variation than the Pl latency. 2. As lengthening progressed, the Pl latency gradually increased, whereas the amplitude decreased. The higher the percentage of lengthening to the original tibial length, the more marked the changes in both the Pl latency and amplitude. 3. As lengthening progressed, significant changes were observed earlier in the amplitude than in the Pl laterncy. 4. Significant changes in the amplitude were observed when lengthening reached 12.2% of the original tibial length in Group I (0.35mm/day) and 10.5% in Group IV (1.4mm/day). Significant changes in the Pl laterncy were observed when lengthening reached 17.8% of the original length. It is concluded that somatosensory evoked potential is an effective method of detecting early and preventing neurological complications in bone lengthening. Somatosensory evoked potential is a clinically feasible technique. It is expected that the finding of study may serve as a useful guideline for detecting the safe limits of velocity and amount of bone lengthening.
Bone Lengthening ; Evoked Potentials, Somatosensory ; Extremities ; Methods ; Paralysis ; Rabbits ; Socioeconomic Factors ; Tibia

Congenital diverticulum of the left ventricle is a very rare disease. Ventricular diverticulum is usually associated with other anomalies including intracardiac and midline thoracoabdominal defect. We describe a case with congenital left ventricular diverticulum presenting as an isolated lesion. A 37-year-old man presented with 4-year history of chest pain. Diverticulum was diagnosed by echocardiography and left ventriculogram.
Adult* ; Chest Pain ; Diverticulum* ; Echocardiography ; Heart Ventricles ; Humans ; Rare Diseases

Anesthesia for the patients with mediastinal mass may be associated with significant respiratory and cardiovaacular complications due to compression of traeheobronchial trees, the pulmonary artery, heart and superior vena cava The authors present a case of a 6-year-old girl with a large mediastinal tumor located in anterior and superior mediaetinum. Preoperatively, the patient was asymptomatic and anesthesia was induced and maintained uneventfully. Operative finding was that 8 X 14 cm sized hemangioma was extended superiorly over thoracic outlet, posteriorly aorta and SVC, medially pericardium and right hilum and inferiorly right upper lung and pleura. Because difficulties in removal of the hemangioma were expected, exploratory thoracotomy was discontinued. On emergence, as soon as the patient was extubated due to irritability, the patient became cyanotic and was noticed bulging mass on right supraclavicular area, Intubation was attempted and during direct laryngoscopy, anesthesiologist also noticed a bulging mass in right oral cavity. Postoperatively, the patients airway was kept with endotracheal tube, but cyanosis appeared on the face and skin over the chest, but not below the abdomen. Bulging mass on right supraclavicular area, distension of neck vein and conjunctival edema were noticed only when the patient was crying or suctioned. Symptoms improved by deep sedation. We assumed that enlargement of hemangioma due to increased central blood volume occasionally compressed tracheobronchial tree and sup. vena cava when patient was irritable, crying and coughing. On 11th postoperative day, patient was transferred to the another hospital for the better treatment with endotracheal intubation.
Abdomen ; Airway Obstruction* ; Anesthesia ; Aorta ; Blood Volume ; Child ; Cough ; Crying ; Cyanosis ; Deep Sedation ; Edema ; Female ; Heart ; Hemangioma ; Humans ; Intubation ; Intubation, Intratracheal ; Laryngoscopy ; Lung ; Mouth ; Neck ; Pericardium ; Pleura ; Pulmonary Artery ; Skin ; Suction ; Thoracotomy ; Thorax ; Veins ; Vena Cava, Superior

Osteoclasts resorb bone by the hydrogen ions and proteolytic enzymes in the localize environment under the ruffled border. Before releasing hydrogen ion and enzymes, osteoclast should attach to bone surface very tightly and make a room to release enzymes and hydrogen ion in the center. Specialized attachment molecule in the cell membrane, such as integrin, is associated with specific noncollagenous protein in the matrix, which has specific amino acid sequence (Arginine-Glycine- Aspartic acid sequence). We may speculate that osteoclast action would be decreased if the integrin is blocked by antibody or RGD protein. In this study, the osteoclasts were cultured on the coverslip or bone slice with or without RGD protein in the culture medium, and numbers of growing giant cells were much less in group with RGD protein. The number resorption pits, formed on mineralized bone slice, was also lower in the group adding RGD protein in the medium. And we made a conclusion that the osteoclastic bone resorption was inhibited by soluble RGD protein.
Amino Acid Sequence ; Aspartic Acid ; Bone Resorption ; Cell Membrane ; Giant Cells ; Osteoclasts* ; Peptide Hydrolases ; Protons

Surgical treatment of congenital scoliosis is often frustrating due to its severity and rigidity. Many surgical methods were provided for these complex deformities and some instruments were used to achieve better correction. However, no surgical treatment was satisfactory to correct the deformities. Recently, pedicle screw fixation, enabling a powerful grip of individual vertebral segment, is becoming a promising method to enhance correction of these tenacious deformities. This study was to compare the correction, loss of correction and complications of the various surgical methods employed for congenital scoliosis in Seoul National University Hospital and to verify the advantages of pedicle screw fixation. Ninety-two congenital scoliosis subjected to surgery and followed up for more than 2 years(range 2-9 years) were analysed. Surgical methods were; anterior uninstrumented fusion, 5; posterior fusion, 50(14 without instrument, 15 with hooks, 21 with screws); combined anterior and posterior fusion, 37(7 without instrument, 11 with posterior hooks, 10 with posterior screws, 9 with anterior VDS and posterior screws). Mean preoperative magnitude of index curve was 58° with no significant difference between the groups except for the anterior VDS and posterior screw group which had a mean magnitude of 93°. Mean flexibility of index curve was 18.7% with no significant difference between groups. The correction of index curves were; anterior uninstrumented fusion, 23%; posterior uninstrumented fusion, 28%; posterior hook, 31%; posterior screw, 46%; combined anterior and posterior uninstrumented, 37%; anterior fusion and posterior hook, 30%; anterior fusion and posterior screw, 47%; anterior VDS and posterior screw, 48%. The correction of pedicle screws were significantly better(p < 0.01) with significantly smaller loss of correction(p < 0.01). Nonunion occurred in 4, 1 in posterior uninstrumented fusion, 2 in posterior hooks and 1 in posterior pedicle screws with concomitant instrument failures. However, there were no neurologic or visceral complications related to screw placement. Pedicle screw fixation offers an enhances correction of congenital scoliosis with low complication rate, and when combined with an anterior procedure, enables control of the most severe and rigid curves.
Congenital Abnormalities ; Hand Strength ; Methods ; Pedicle Screws ; Pliability ; Scoliosis ; Seoul

During anesthesia and surgery, body homeostasis and tissue oxygen demand/supply balance is disrupted and blood lactate concentration in increased. We have studied the changes of blood lactate concentrations and arterial, venous oxygen contents during open heart surgery in 15 patients. Samplings were done at 1) before anesthetic induction, 2) after induction, 3) after sternotomy, 4) onset of cardiopulmonary bypass, 5) duing hypothermic cardiopulmonary bypass, 6) after cardiopulmonary bypass and 7) after operation. Blood lactate concentrations were inereased signifieantly at the onset of, during and after cardiopulmonary bypass and after operation(p<0.001). Arterial oxygen contents were decreased significantly at the onset of, during and after cardiopulmonary bypass(p<0.05). Venous oxygen contents were decreased significantly at the during and after cardiopulmonary bypass and after operation(p<0.05). There were significant negative correlations between arterial, venous oxygen contents and blood lactate concentrations. Changes of venous oxygen saturation, mean arterial pressure and pH had not significant relationships with the lactate concentrations.
Anesthesia ; Arterial Pressure ; Cardiopulmonary Bypass ; Heart* ; Homeostasis ; Humans ; Hydrogen-Ion Concentration ; Lactic Acid* ; Oxygen ; Sternotomy ; Thoracic Surgery

Serum aspartate aminotransferase (AST) is a common enzyme for the evaluation of the hepatic, muscular and cardiac diseases and is produced also at kidney, brain, pancreas, lung, leukocytes, erythrocytes, etc. The elevation of its activity is usually caused by the necrosis of hepatocytes when there are not muscular injuries or myopathies. Recently, it is found that AST can exist as a macroenzyme by forming a complex with an immunoglobulin and this complex is erroneously considered to indicate the presence of liver disease as a result of elevation of AST activity on routine blood chemistry analysis. We experienced the patient with isolated AST elevation due to the formation of AST-mmunoglobulin complex confirmed by AST isoenzyme electrophoresis (EP).
Aspartate Aminotransferases ; Brain ; Chemistry ; Electrophoresis ; Erythrocytes ; Heart Diseases ; Hepatocytes ; Humans ; Immunoglobulins ; Kidney ; Leukocytes ; Liver Diseases ; Lung ; Muscular Diseases ; Necrosis ; Pancreas

BACKGROUND: Two tumor suppressor genes, p53 and p16, which have different roles in controlling the cell cycle and inducing apoptosis, are frequently inactivated during carcinogenesis including lung cancer. Single tumor suppressor gene therapies using tither with p53 or p16 have been studied extensively. However, there is a paucity of reports regarding a combined gene therapy using these two genes. METHODS: The combined effect of p53 and p16 gene transfer by the adenoviral vector on the growth of lung cancer cell lines and its interactive mechanism was investigated. RESULTS: An isobologram showed that the co-transduction of p53 and p16 exhibited a synergistic growth inhibitory effect on NCI H358 and an additive effect on NCI H23. Cell cycle analysis demonstrated the induction of a synergistic G1/S arrest by a combined p53 and p16 transfer. This synergistic interaction was again confirmed in a soft agar clonogenic assay. CONCLUSION: These observations suggest the potential of a p53 and p16 combination gene therapy as another potent strategy in cancer gene therapy.
Adenoviridae ; Agar ; Apoptosis ; Carcinogenesis ; Cell Cycle ; Cell Line* ; Genes, Neoplasm ; Genes, p16 ; Genes, Tumor Suppressor ; Genetic Therapy ; Lung Neoplasms* ; Lung*