OBJECTIVE To develop a rapid method for simultaneously identifying Staphylococcus aureus(SA) and its resistance against meticillin. METHODS The target strains authenicated to Staphylococcus by traditional methods(appearance,Gram-stain,catalase) first,then using Slidex~staph.plus to authenticate SA;establishing traditional method or coagulase test and ATB-ID32STAPH system for verification.The penicillin-binding protein 2a(PBP2a) was examined by Slidex MRSA Detection,establishing resistance oxacillin sieving test and mecA gene was examined by PCR for verification. RESULTS The 135 strains were positive and 321 strains were negative for Slidex~staph.plus from 456 strains.The 127 S.aureus strains and eight others were confirmed from 135 positive strains finally,11 SA strains and 310 other strains were confirmed from 321 negative strains,there were 92.0% for sensitivity and 97.5% for specificity in this method.The 52 strains PBP2a positively confirmed to meticillin-resistant S.aureus(MRSA) using Slidex MRSA Detection and 57 MRSA strains were confirmed using resistance oxacillin sieving test or PCR from 138 strains.There were 91.2% for sensitivity and 100% for specifity in this method. CONCLUSIONS The duplex Slidex~-staph monoclonal antibody examinated to SA and confirmed to MRSA has higher sensitivity and specificity.

The anti-fatigue ability decline is one of the most important clinical symptoms of chronic exertional compartment syndrome (CECS). The percentage change of type-I and II skeletal-muscle fiber is an important reason for anti-fatigue ability decline after intracompartmental pressure increase. There is a close relationship between CaN-NFAT or PPAR/PGC-1 signaling pathways and muscle fiber type conversion. Studies have confirmed that Traditional Chinese medicine can protect the body tissue by activing CaN-NFAT or inhibiting PPAR/PGC-1 signaling pathways. Therefore, we wrote the review in order to better analyze the research progress in this field.

Objective: To examine the impact of laser-assisted hatching (LAH) technique on perinatal outcomes in blastocyst culture of low-grade cleavage embryos, so as to provide insights into improving the utilization of low-grade cleavage embryos and embryo quality. Methods: A total of 369 single live births after transfer of thawed blastocysts following in vitro fertilization or intracytoplasmic sperm injection at Zhejiang Provincial People's Hospital were selected as subjects, and they were divided into 51 conventional culture blastocysts and 318 LAH blastocysts based on whether LAH was performed on day 4 of blastocyst culture. Gestational age, birth weight, birth defects and maternal perinatal information were collected, and the prevalence of premature birth, birth weight and birth defects were analyzed after propensity score matching (PSM). Results: After PSM, 98 matched cases were included in the study. There were no statistically significant differences in maternal age, body mass index, type of infertility and blastocyst age (P>0.05), indicating effective matching. The birth weight of offspring in the LAH group was lower than that in the conventional culture group [(3 261.08±432.24) g vs. (3 464.18±444.46) g; P<0.05]. Conclusion The birth weight of offspring can be reduced by using LAH during blastocyst culture of low-grade cleavage embryos.

Objective:To analyze the evaluation value of changes in β-human chorionic gonadotrophin (β-HCG) for the curative effect of methotrexate (MTX) on ectopic pregnancy (EP).Methods:A retrospective analysis was performed in 80 EP patients who admitted to Jinhua People's Hospital from January 2013 to August 2019.Results:Of the 80 EP patients, success and failure rates of treatment were 77.50% (62/80) and 22.5% (18/80), respectively.According to different treatment results, they were divided into success group (62 cases) and failure group (18 cases). Before treatment, hemodynamic index of fallopian tube artery[resistance index (RI)] in success group was higher than that in failure group ( t=4.150, P<0.05). Before treatment, after 1d, 4d and 7d of treatment, the serum levels of β-HCG in success group were lower than those in failure group ( t=69.496, 76.618, 217.488, 310.203, all P<0.05). The results of Pearson correlation analysis showed that RI was negatively correlated with serum level of β-HCG before treatment, after 1d, 4d and 7d of treatment ( r=-0.374, -0.396, -0.471, -0.482, all P<0.05). The receiver operating characteristic (ROC) curves analysis showed that after 4d and 7d of treatment, areas under the curve (AUC) of β-HCG for evaluating the curative effect of MTX on EP were 0.821 and 0.829, respectively, which were higher than before treatment and after 1d of treatment (0.685, 0.701) ( P<0.05). Conclusion:The changes of β-HCG are of high clinical value in evaluating the curative effect of MTX on EP, which can be applied as an important monitoring index.