Objective To establish the rabbit brain death model,and observe the change of kidney function and morphology in the brain death rabbits,fully evaluate the possibility of transplantation surgery carried out with brain death donor kidney.Method 40 male healthy New Zealand rabbits were randomly divided into sham group (n =20) and brain death group (n =20),and each group further was divided into four groups according to 2,4,6 and 8 h after brain death groups.At the end of the experiment,the blood samples and kidney tissues were collected.The level of blood urea nitrogen(BUN),serum creatinine (Cr) and the apoptosis of kidney cells were determined by automatic biochemistry analyzer and TUNEL method,respectively.The morphology changes of liver were detected by HE staining.Result Although BUN values were with no obviously alteration (P＞0.05) within 8 h after brain death,the renal Cr levels in the states of 2,4 and 8 h (P＜0.05) brain death were found significantly increased compared with shamed groups.The denaturalization in the epithelial cells of renal tubule,glomerulus atrophygradually,swelling and vacuolation of epithelial cells,edema in the interstitium and inflammatory cell infiltration and partly occlusion in proximal convoluted tubule gradually emerged in the brain dead groups.The inflammatory factor intercellular adhesion molecule (ICMA) and the apoptosis index in brain dead groups increased in a time dependent manner but the expressions of protective factor HSP70 conducted a contrary tendency.Conclusin Brain death could lead to the damage of kidney function and morphology and this injury aggravated in a time dependent manner.The alterations might be relevant to the production of inflammatory factors.Within 8 h after brain death there were obvious changes in the kidney function and morphology,which provide experimental basis for the rational use of brain death donor kidney transplantation.

Objective To provide theoretical reference and practical guidance for clinical nursing through Meta-analysis method which is applied to explore the conventional nursing method and improvement intervention nursing methods to prevent liver transplantation the effect of pressure sores. Methods Databases including the Cochrane Library, PubMed, EMbase, OVID, China National Knowledge Infrastructure ( CNKI) , Wanfang Data and CBM, were searched by computer to collect the randomized controlled trails and quasi randomized controlled trials about the conventional nursing method and improvement intervention nursing methods for pressure sores of liver transplantation. Chinese Journal of Nursing, Chinese Journal of Modern Nursing, Journal of Nurses Training were searched by hand.The retrieval time was from February First, 1966 to February First, 2016. The studies were screened according to inclusion and exclusion criteria. The data were extracted and the quality was evaluated by two reviewers independently. Meta-analysis was conducted by RevMan 5.2 software.Results A total of 12 controlled trails involving 1 245 patients were included in this research, and there were 623 patients in the intervention group, and 622 patients in the control group. The results of meta-analysis showed that:the rate of pressure scores in intervention group was lower than the control group [ OR=0.25,95% CI(0.16,0.38),P<0.01],[RR=0.29,95% CI(0.20,0.42),P<0.01]. The overall severity of pressure scores in the intervention group was significantly lower than that in the control group[OR=0.34,95%CI(0.16,0.73),P<0.01],[RR=0.57,95% CI(0.37,0.87),P<0.01].Conclusions In the intervening group the rate of pressure ulcer and the overall severity of pressure scores was significantly lower than that in the control group, which shows that the intervention nursing method can effectively reduce the occurrence and stage of pressure scores in liver transplant patients and reduce the severity of pressure scores.

The human resource management of organ donation coordinators in China is still in its infancy stage, plagued by such problems as unclear career orientation, poor management and unclear career planning. In March 2010, a tertiary public hospital was approved as a medical institution in a national pilot province for organ donation. In recent years, the hospital had kept exploring human resource management of coordinators and established a relatively complete management mode for organ donation coordinators. This mode featured the establishment of full-time recruitment positions, development of human resource management plans, refinement of job descriptions, establishment of performance evaluation plans, optimization of assessment and incentive mechanisms, and innovation of talent cultivation modes. The management practice had achieved certain results, ensuring the sustainable development of hospital organ donation operation, and providing a reference for the scientific and standardized development of organ donation and transplantation in China.